Abstract
The aim of this study was to identify Candida species isolated from women diagnosed with recurrent vulvovaginal candidiasis (RVVC) and their partners; and to evaluate the fluconazole (FLZ) susceptibility of the isolates. In a period of six years, among 172 patients diagnosed with vulvovaginal candidiasis, 13 women that presented RVVC and their partners were selected for this investigation. The isolates were obtained using Chromagar Candida medium, the species identification was performed by phenotypic and molecular methods and FLZ susceptibility was evaluated by E-test. Among 26 strains we identified 14Candida albicans, six Candida duobushaemulonii, four Candida glabrata, and twoCandida tropicalis. Agreement of the isolated species occurred in 100% of the couples. FLZ low susceptibility was observed for all isolates of C. duobushaemulonii (minimal inhibitory concentration values from 8-> 64 µg/mL), two C. glabrataisolates were FLZ-resistant and all C. albicans and C. tropicalis isolates were FLZ-susceptible. This report emphasises the importance of accurate identification of the fungal agents by a reliable molecular technique in RVVC episodes besides the lower antifungal susceptibility profile of this rare pathogen C. duobushaemulonii to FLZ.
Highlights
Vulvovaginal candidiasis (VVC) is a very common infection that affects a great number of women at reproductive age and the frequent cause of taking gynecological medical consultation
From July 2005 to August 2011, 2,026 female patients ranging from 18-65 years old were evaluated at Gynecologic Services of three private and two public Services in São Paulo city, São Paulo state, Brazil
C. albicans (14), Candida duobushaemulonii (6), C. glabrata (4), and C. tropicalis (2) isolates were identified by phenotypic characteristics and confirmed by molecular methods
Summary
Vulvovaginal candidiasis (VVC) is a very common infection that affects a great number of women at reproductive age and the frequent cause of taking gynecological medical consultation. Candida albicans is the most common causal agent but non-albicans species have been identified (Richter et al 2005, Sobel 2007). C. glabrata isolates present lower azoles susceptibility than other species (Richter et al 2005, Sobel 2007).
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