Chicken Eggshell Membrane Research Articles

Nutrien dan Morfologi Membran Kerabang Telur Ayam Kampung sebagai Bahan Baku Produksi Kolagen

Collagen derived from the eggshell membrane, is an increasingly popular functional material. Native chicken is one of the leading local commodities whose industrial waste has the potential to be used as collagen raw material. This study aimed to evaluate the nutrients and morphology of the eggshell membrane of native chicken eggs as raw material for collagen production. The parameters observed were morphology using electron scanning microscopy, mineral composition, and collagen nutrient content (water and protein). SEM observations showed that the eggshell membrane of native chicken eggs was fibrous. The meshed fiber form represents the extracellular matrix present in collagen proteins. The eggshell membrane contains Cl, K, Ca, P, and Mg macrominerals at 1.696%, 1.796%, 14.30%, 0.437%, and 0.146%, respectively. Microminerals I and Mo were also found at 0.00034% and 0.00014%, respectively. The moisture content of the superior native chicken eggshell membrane decreased by 13.05% after the collagen extraction (p<0.05), with a crude protein content of 25.32% after the extraction. It was concluded that the eggshell membrane of native chicken eggs produces fibrous morphological characteristics that are meshed and contain high minerals so that they have the potential to be used as raw materials for collagen production in various applications.
 
 Keywords: collagen, eggshell, eggshell membrane, native chicken, morphology

Synthesis and Assessment of Antimicrobial Composites of Ag Nanoparticles or AgNO3 and Egg Shell Membranes.

Engineering research has been expanded by the advent of material fusion, which has led to the development of composites that are more reliable and cost-effective. This investigation aims to utilise this concept to promote a circular economy by maximizing the adsorption of silver nanoparticles and silver nitrate onto recycled chicken eggshell membranes, resulting in optimized antimicrobial silver/eggshell membrane composites. The pH, time, concentration, and adsorption temperatures were optimized. It was confirmed that these composites were excellent candidates for use in antimicrobial applications. The silver nanoparticles were produced through chemical synthesis using sodium borohydride as a reducing agent and through adsorption/surface reduction of silver nitrate on eggshell membranes. The composites were thoroughly characterized by various techniques, including spectrophotometry, atomic absorption spectrometry, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy, as well as agar well diffusion and MTT assay. The results indicate that silver/eggshell membrane composites with excellent antimicrobial properties were produced using both silver nanoparticles and silver nitrate at a pH of 6, 25 °C, and after 48 h of agitation. These materials exhibited remarkable antimicrobial activity against Pseudomonas aeruginosa and Bacillus subtilis, resulting in 27.77% and 15.34% cell death, respectively.

Therapeutic Application of an Ag-Nanoparticle-PNIPAAm-Modified Eggshell Membrane Construct for Dermal Regeneration and Reconstruction.

Current therapeutic treatments for the repair and/or replacement of damaged skin following disease or traumatic injury is severely limited. The chicken eggshell membrane (ESM) is a unique material: its innate physical and mechanical characteristics offer optimal barrier properties and, as a naturally derived extract, it demonstrates inherent biocompatibility/biodegradability. To further enhance its therapeutic and clinical potential, the ESM can be modified with the thermo-responsive polymer, poly(N-isopropylacrylAmide) (PNIPAAm) as well as the incorporation of (drug-loaded) silver nanoparticles (AgNP); essentially, by a simple change in temperature, the release and delivery of the NP can be targeted and controlled. In this study, ESM samples were isolated using a decellularization protocol, and the physical and mechanical characteristics were profiled using SEM, FT-IR, DSC and DMA. PNIPAAm was successfully grafted to the ESM via amidation reactions and confirmed using FT-IR, which demonstrated the distinctive peaks associated with Amide A (3275 cm−1), Amide B (2970 cm−1), Amide I (1630 cm−1), Amide II (1535 cm−1), CH2, CH3 groups, and Amide III (1250 cm−1) peaks. Confirmation of the incorporation of AgNP onto the stratified membrane was confirmed visually with SEM, qualitatively using FT-IR and also via changes in absorbance at 380 nm using UV-Vis spectrophotometry during a controlled release study for 72 h. The biocompatibility and cytotoxicity of the novel constructs were assessed using human dermal fibroblast (HDFa) and mouse dermal fibroblast (L929) cells and standard cell culture assays. Metabolic activity assessment (i.e., MTS assay), LDH-release profiles and Live/Dead staining demonstrated good attachment and spreading to the samples, and high cell viability following 3 days of culture. Interestingly, longer-term viability (>5 days), the ESM-PNIPAAm and ESM-PNIPAAm (AgNP) samples showed a greater and sustained cell viability profile. In summary, the modified and enhanced ESM constructs were successfully prepared and characterized in terms of their physical and mechanical profiles. AgNP were successfully loaded into the construct and demonstrated a desirable release profile dependent on temperature modulation. Fibroblasts cultured on the extracted ESM samples and ESM-PNIPAAm demonstrated high biocompatibility in terms of high cell attachment, spreading, viability and proliferation rates. As such, this work summarizes the development of an enhanced ESM-based construct which may be exploited as a clinical/therapeutic wound dressing as well as a possible application as a novel biomaterial scaffold for drug development.

Parameter Screening and Optimization for a Polycaprolactone-Based GTR/GBR Membrane Using Taguchi Design.

Our objective was to determine and optimize the significant parameters affecting mechanical properties and mean fiber diameter (MFD) of a novel GTR/GBR membrane composed of polycaprolactone (PCL) and chicken eggshell membrane (ESM). For this, we prepared electrospun membrane specimens (n = 16) with varying concentrations of PCL, ESM, nano-hydroxyapatite (HAp), and altered electrospinning parameters as generated by DOE++ software. After the determination of MFD and mechanical properties for all specimens, Taguchi orthogonal array L8 design was used to screen significant factors affecting the MFD and mechanical properties. PCL wt%, ESM wt%, HAp wt%, applied voltage (AV), flow rate (FR), and spinneret-collector distance (SCD) were the independent variables investigated. The response variables analyzed were MFD, tensile strength (TS), and elastic modulus. ANOVA outlined ESM wt%, HAp wt%, AV, FR, SCD, and an interactive effect between PCL wt% and AV to be the significant factors affecting modulus values of an electrospun PCL/ESM membrane (p < 0.05). Furthermore, concentrations of PCL and ESM were the significant factors affecting MFD (p < 0.05) and there were no significant factors affecting the TS values. Optimization using DOE++ software predicted that the maximal TS of 3.125 MPa, modulus of 278.168 MPa, and MFD of 882.75 nm could be achieved.

KARAKTERISASI SIFAT ELEKTROKIMIA ELEKTRODA KARBON AKTIF BERBASIS LIMBAH SABUT KELAPA MUDA MENGGUNAKAN SEPARATOR MEMBRAN KULIT TELUR AYAM

The supercapacitor is an energy storage device with an electrochemical characteristic that can provide high energy and power density. The supercapacitor cell consists of a current collector, electrode, electrolyte, and separator. This study used young coconut fiber biomass as a supercapacitor electrode, H 2 SO 4 as an electrolyte, 316L stainless steel as a current collector, and chicken eggshell membrane as a separator. The fabrication of electrode carbon made from young coco fiber begins with the pre-carbonization process, chemical activation using 0.5M KOH, carbonization process using N 2 gas at a temperature of 600 °C, and physical activation using CO 2 gas at a temperature of 750 °C. Analysis of electrochemical characteristics using cyclic voltammetry method resulting in a specific capacitance value of 197.05 F/g for a scan rate of 1 mV/s, 157.215 F/g for a scan rate of 2 mV/s, and 129.42 F/g for scan rate of 5 mV/s.

Development of Bioactive Glass-Collagen-Hyaluronic Acid-Polycaprolactone Scaffolds for Tissue Engineering Applications.

In this work, bioactive glass (BG) particles synthesized by a sol-gel method, hyaluronic acid (HYA) and collagen (COL) extracted from chicken eggshell membrane (ESM), and as-purchased polycaprolactone (PCL) were used to obtain a novel bioactive scaffold using the gel-pressing technique. Two composite mixtures in weight percent were obtained and identified as SCF-1 and SCF-2, and were characterized by using FTIR, XRD, and SEM techniques. Subsequently, the composite materials applied as coatings were evaluated in simulated body fluid solutions using electrochemical techniques. The results of bioactivity and biodegradability evaluations, carried out by immersing in simulated body fluid and phosphate-buffered saline solution, showed that the SCF-1 sample presented the best biocompatibility. In accordance with the potentiodynamic results, the 316L-SS and the SCF-1-coated SS showed a very similar corrosion potential (E corr ), around −228 mV, and current density (i corr ) values in close proximity, while the SCF-2-coated SS showed more positive E corr around −68 mV and lower i corr value in one order of magnitude. These results agree with those obtained by electrochemical impedance spectroscopy, which show a corrosion mechanism governed by activation and finite diffusion through the porous layer. In addition, results were complemented by dynamic compression testing under oscillating forces to identify the developed scaffolds’ response under external forces, where the SCF-1 scaffold presented a maximum compression. The degradation resistance, bioactivity, and mechanically obtained measurements provided interesting results for potential further studies in tissue engineering.

Anti-Aging Face Cream Preparations Made from Collagen Membrane Chicken Eggshell (Gallus gallus domesticus) Combined with Bee Honey (Apis mellifera)

Handling waste such as chicken egg shells requires innovation to be able to provide added value as a sustainable product. This study aims to extract collagen from chicken egg shell membranes combined with bee honey as an anti-aging face cream. This material has a very high model accuracy value (97.89%) which has the potential to inhibit aging due to Matrix metalloproteinase-1 (MMP-1). This research was carried out by extracting eggshell membrane collagen, making cream combined with bee honey, analyzing product properties, and interpreting virtual data. The results show that in 100 g of face cream formulation with active ingredients of chicken eggshell membrane collagen extract 4.1206% and bee honey 5.0251%; 77.0926% water; 0.0062% ash; 1,8690% protein; 0.0058% fat, the viscosity value was 65.94 dPas, and the pH was 7.08. Thus, this product did not irritate the skin of the experimental animals. The average value of attribute assessment (such as aroma, consistency, texture, stickiness, homogeneous sensation, color, appearance, itching, erythema, washing power, response after washing) was well received by the panelists with a score of 1-3,9 and the highest total achievement bacteria 25 CFU/g and yeast-mold 16 CFU/g met the skin moisturizer quality requirement (SNI 16-4399-1996) which should not exceed 102 CFU/g.&#x0D; Key words: collagen; Matrix metalloproteinase-1; honey; extraction; organoleptic

Collagen face spray: facial moisturizer from chicken egg shell membrane to prevent premature aging

Skin is often exposed to various factors, so the premature aging process become one of the public’s concerns. One of the efforts to maintain skin beauty and prevent premature aging is to fulfil the intake of collagen in the skin. Collagen is a protein, which one is found at chicken egg shells membrane. So far, chicken egg shells are only seen as waste, pollute the environment and are not used properly. Therefore, this innovation to making face spray cosmetic products appears to be a new breakthrough in the utilization and waste treatment. It can function to moisturize and nourish the skin, soothe facial skin, anti-aging, relieves redness on sensitive skin and can give a smooth impression on makeup. The method used in this research is an experiment, the results based on anatomical pathology tests using microscopic 400x magnification with Sirius red staining obtained 52.8% collagen content in egg shell membrane, and pH test obtained value of pH 5.4 which meets the requirements for the safe value for the skin pH 4.5-6.5 and the results of the organoleptic test on collagen face spray product are odorless, colorless, and the material is in the form of a liquid face spray cosmetic.

Beneficial effect on rapid skin wound healing through carboxylic acid-treated chicken eggshell membrane

At the initial stage of wound healing, growth factors stimulate tissue regeneration by interacting with the extracellular matrix (ECM), leading to rapid wound repair and structural support. Chicken eggshell membrane (ESM) is a low-cost and highly functional ECM biomaterial for tissue regeneration. However, natural ESM has limitations for tissue engineering purposes because it is difficult to control the size, shape, and biocompatibility of the surfaces. To overcome this, blends of synthetic materials and natural ESMs, such as soluble eggshell membrane protein, are combined for biomaterial applications. Unfortunately, it is difficult to pattern fibrous structure. Here, we modified the natural chicken ESM through weak acid treatment to promote wound healing and skin regeneration without loss of fibrous structure. Treatment of citric acid and acetic acid reacted the amine or amide group with carboxyl groups (R-COOH) and achieved hydrophilicity for adherence of proliferating regenerative cells. Our in vitro study revealed that the modified ESM scaffolds significantly promoted human dermal fibroblasts adhesion, viability, proliferation, and cytokine secretion, compared with natural ESM. In addition, the modified ESM accelerated skin regeneration and enhanced the wound healing process even at early stages in an in vivo rat wound model. Collectively, the modified ESM performed best for promoting skin regeneration, cytokine secretion, epidermal cell proliferation, and controlling the inflammatory response both in vitro and in vivo.

Application of Neutron-Irradiated &lt;sup&gt;6&lt;/sup&gt;Li (n, α) &lt;sup&gt;3&lt;/sup&gt;H Reaction to a Protein-Based Fibrous Non-Woven Fabric Biopolymer: Radiolabeling of Cross-Linked Natural Fibrous Chicken Eggshell Membranes and Their Whole-Body Tissue Distribution after Oral Ingestion in Mice

Application of Neutron-Irradiated &lt;sup&gt;6&lt;/sup&gt;Li (n, α) &lt;sup&gt;3&lt;/sup&gt;H Reaction to a Protein-Based Fibrous Non-Woven Fabric Biopolymer: Radiolabeling of Cross-Linked Natural Fibrous Chicken Eggshell Membranes and Their Whole-Body Tissue Distribution after Oral Ingestion in Mice

Mildly Processed Natural Eggshell Membrane Alleviates Joint Pain Associated with Osteoarthritis of the Knee: A Randomized Double-Blind Placebo-Controlled Study.

Poor joint health is a significant burden to society. Millions of people suffer from some form of joint-related disorder or disease, most often osteoarthritis (OA). It was hypothesized that chicken eggshell membrane (EM) is effective in the regeneration of cartilage and/or immunomodulation (oral tolerance), and as such relieves pain and stiffness in joints commonly affected in arthritis. We tested this hypothesis in a double-blind, placebo-controlled EM intervention study. Of 150 male and female volunteers, 40–75 years of age and diagnosed with knee OA, 75 were randomly assigned to the EM intervention group and 75 to the placebo group. During 12 weeks, subjects received a daily capsule containing either 300 mg of EM or a placebo. The main primary dependent variable consisted of self-reported pain ratings on a Numerical Rating Scale Pain (NRS-P) 6 weeks after study start. As secondary dependent variables served NRS-P scores collected after 12 weeks, and Knee injury and self-reported Osteoarthritis Outcome Scores (Knee injury and Osteoarthritis Outcome Scores [KOOS]). NRS-P scores decreased for both groups at approximately the same rate, but only EM relieved self-reported pain scores obtained with the KOOS questionnaire starting 1 week after initiation of treatment. This effect was significant for two of five KOOS category scores, that is, “Pain” and “Daily Life” functioning, aggregate pain, and functioning scores composed of complaint ratings for a wide variety of daily activities. These scores showed long-lasting improvement, and demonstrated that EM extract successfully reliefs knee OA pain and contributes to daily life functioning.

Eggshell membranes coated chitosan decorated with metal nanoparticles for the catalytic reduction of organic contaminates

This study focusses on the effect of chitosan coating with eggshell membranes for the reduction of different organic pollutants. Chickens eggs were collected from the local market and utilized to extract the enrich eggshell membranes (ESM). The chicken eggshell membranes are abundant waste material which is inexpensive and illustrates remarkable physiognomies for many possible applications. Fresh fibers/strips coated by chitosan (CS) were prepared by mixing the eggshell membranes with CS solution (2 wt%/v) in different proportions i.e., 10 %, 30 %, 50 %, 60 %, 70 %, 80 %, and 90 %. These strips were then templated with copper and iron metal nanoparticles by putting them in their metal ions aqueous solution to adsorb the metals ions and were then reduced to zero-valent metal nanoparticles (MNPS) by using NaBH4 aqueous solution. These prepared materials (MNPS@ESM-CS) were characterized by using XRD, XPS, FE-SEM, and EDS to confirm the successful preparation of MNPs over the surface of ESM coated with CS. Afterwards, these prepared materials were investigated as a catalyst for the reduction of different organic pollutants, such as 4-nitroaniline (4-NA), 4-nitrophenol (4-NP) and methylene blue (MB) dye. The catalytic efficiency of ESM was enhanced 5.7-fold by adding only 20 % CS solution. It was observed that Cu@ESM-CS-80 % took 7 min for reduction of 4-NA, 6 min for 4-NP, and 7 min for MB dye. The reusability of the catalytic strip was also investigated for four cycles and found efficient and can be easily recovered by simply pulling it from the reaction mixture.

Turning waste into treasure: chicken eggshell membrane derived fluorescent carbon nanodots for the rapid and sensitive detection of Hg2+ and glutathione.

Herein, a green, economical, and waste-utilization approach is reported for the synthesis of water-soluble carbon nanodots (C-Dots) with a high fluorescence quantum yield of 19.5%. As a common protein-rich waste, eggshell membrane was selected as a cost-effective and ideal precursor to prepare C-Dots using the microwave method. The as-prepared C-Dots showed a maximum emission at 375 nm with an excitation wavelength at 235 nm. The fluorescent C-Dots were adopted as a sensitive probe for the rapid detection of Hg2+ and glutathione (GSH) based on the fluorescence off and on (turn-off-on) strategy. This was ascribed to the fact that Hg2+ could effectively quench the fluorescence of the C-Dots and GSH was able to prevent fluorescence quenching owing to the specific binding between Hg2+ and GSH. The designed method exhibited a high sensitivity and selectivity towards the detection of Hg2+ and GSH. Under the optimized conditions, the method showed a good linear relationship with Hg2+ concentration in the range from 100 nM to 50 μM with a detection limit of 32.0 nM. For GSH detection, it displayed a linear range from 50 nM to 10 μM with a detection limit of 9.8 nM. Moreover, this method was successfully applied to detect GSH in human serum samples. The eggshell derived fluorescent C-Dots pave the way for economical environmental and biological analyses.

3D printing‐assistedcombinatorial approach for designing mechanically‐tunable and vascular supportive nanofibrous membranes to repair perforated eardrum

AbstractPerforation of eardrum or tympanic membrane (TM) is a common clinical condition, which occurs due to infection or injury of the eardrum, and could results in varying degrees of conductive hearing loss among all ages. In this study, the authors report the combinatorial approach of designing mechanically‐tunable and vascular supportive nanofibrous membranes by 3D printing‐assisted electrospinning (e‐spin) using polycaprolactone (PCL) and gelatin with different mass ratios suitable to repair a perforated eardrum. The physicochemical, mechanical, and biological properties of the membranes were characterized. The results show that the membrane has nanofibrous morphology with fibers are of varying size (400–600 nm in diameter) depending on processing conditions. The wettability and mechanical properties of the membrane can be tuned by regulating the gelatin content. Moreover, a biomimetic repair strategy inspired by chicken eggshell membrane, often used in wound dressings, was also presented for study and results show that the suture retention strength of the fabricated membrane can meet clinical translational requirements to promote TM healing. The vascular cell responsiveness of PCL/gelatin nanofibrous membrane was evaluated using human umbilical vein endothelial cells (HUVECs) and the results showed satisfactory biocompatibility, vascular cell responsiveness, and cell proliferation. The findings of this study demonstrate that the combinatorically engineered PCL/gelatin nanofibrous membrane has great potential for repairing perforated eardrum.

Effect of Membrane Removal on the Production of Calcium Oxide from Eggshells via Calcination

In the pursuit of a circular economy and against a background of viable alternative uses of avian eggshells and eggshell membranes, this research investigated the effect of domesticated chicken eggshell membrane separation on the calcination process and quality of Calcium Oxide (CaO) produced. Acetic acid was used to leach the eggshells for 60 min to aid separation of the shell membrane. X-ray diffraction, red green blue colour model, and thermogravimetric analyses were used to characterize the calcined shells. Leached and separated shells produced CaO of comparable quality and crystallinity to unseparated shells after calcination. Results showed that while leaching and membrane separation did not affect calcination temperature, residual ash from the membrane left impurities in the unseparated shells after calcination. It was concluded that leaching and separating the membranes from eggshells prior to calcination serves to upcycle eggshells by facilitating improved CaO production and membrane value extraction.

New approaches of curing and degradation on epoxy/eggshell composites

Epoxy resin suitable for structural composite based on bisphenol A diglycidyl ether (DGEBA) was mixed with anhydride methyl tetrahydrophthalic (MTHPA) as curing agent. As catalyzer two systems were investigated, the synthetic 2,4,6-tris(dimethylaminomethyl)phenol (DEH 35), and the natural based on chicken eggshell (E) and chicken eggshell membrane (M). The influence of DEH 35, E and M on the thermal stability and curing behaviors were evaluated by thermogravimetry (TGA), differential scanning calorimetry (DSC), and Fourier transform infrared spectroscopy (FTIR). Increasing the heating rates “S” curing plots were displayed to higher temperatures, whereas under higher catalyzer content the curing started at lower temperatures. Based on FITR spectra, consuming and development of main chemical groups connected with epoxy curing were precisely followed, corroborating TG and DSC data. Proper curing rate, thermal stability and degradation rates were reached in epoxy upon EM and M addition suggesting improved cross-linked compounds. Summing up, this work presents the curing kinetics and degradation analyses of epoxy/eggshell composites, thermodynamics and kinetics parameters are reported as well as the main molecular groups involved with phase transitions, which can help to understand and control both mechanisms.

A Randomized, Double-Blind, Placebo-Controlled, Prospective Clinical Trial Evaluating Water-Soluble Chicken Eggshell Membrane for Improvement in Joint Health in Adults with Knee Osteoarthritis.

Osteoarthritis (OA) is a chronic condition that impacts quality of life and functionality for which consumers often seek dietary supplements to provide some relief. The purpose of this double-blind, placebo-controlled clinical trial was to assess the safety and efficacy of a water-soluble chicken eggshell membrane hydrosylate (WSEM) dietary supplement (BiovaFlex®) 450 mg daily on knee function, mobility, and general health and well-being in 88 adults with OA randomized into intervention (n = 44) or placebo (n = 44) groups. Outcomes were assessed periodically over 12 weeks, including the Western Ontario McMaster Osteoarthritis Index (WOMAC), the six-minute walk test (6MWT), knee range of motion (ROM) testing, and safety. Normalized analysis (improvement over baseline) showed that the poorest initial performers benefited the greatest from the WSEM by day 5 in the 6MWT, with the rest of the population showing significant improvement over placebo by week 12. The normalized WOMAC Stiffness score was also significantly improved over placebo by day 5 (P < .05). Without normalization, no statistically significant improvements were seen in WOMAC, 6MWT, and ROM testing. The Product was also found to be safe in this study. In conclusion, daily consumption of WSEM significantly enhanced average individual physical capacity (walking distance and ability), reduced stiffness by the fifth day of supplementation with the greatest benefit seen by the most compromised individuals, and was maintained over 12 weeks. A WSEM dietary supplement may offer a safe option for relief from symptoms and increased mobility for those with OA.

Development of eggshell membrane as an alternative for black triangle reconstruction following orthodontic tooth movement

Improper tissue alterations can occur following orthodontic treatment. One of such alterations is the opening of the interdental papilla, the so-called “black triangle” (BT), which can affect smile appearance and lead to functional problems. Use of hyaluronic acid (HA) to treat BT is an innovation that has significant results. The newest sources of HA can be extracted from the chicken eggshell membrane (ESM), which might have the potential to treat BT as effectively as commercial HA. The aim of this study was to investigate the effectiveness of ESM containing HA in treating BT. Thin-layer chromatography (TLC) was used to detect HA isolated from ESM, while Fourier-transform infrared spectroscopy (FTIR) analysis was performed to confirm the formation of HA. A total of 18 Cavia cobaya were equally divided into two groups, treatment (10% HA injection) and control (phosphate-buffered saline), to confirm the efficacy of the materials in vivo. Parameters obtained from clinical observations of the differences between ligature-papilla distances (LPD) and socket-papilla distances (SPD) measurement was compared with independent t-tests. The FTIR analysis showed that HA was successfully identified from the ESM samples. This was indicated by the adsorption –OH band at 3419 cm−1, the–COOH band at 1634 cm−1, and the C–O–C saccharide unit at 1049 cm−1. Moreover, HA was also detected from ESM under UV light at 254 nm using thin layer chromatography (TLC) analysis. Local injection of 10% HA induced an augmentation effect of the interdental papilla compared to that in the control group (P<.05). The results of this study reveal that ESM containing HA has the potential effect to regenerate the interdental papilla construction after orthodontic tooth movement.

Solubilized eggshell membrane supplies a type III collagen-rich elastic dermal papilla

Signs of aging in facial skin correlate with lifespan and chronic disease; however, the health of aging skin has not been extensively studied. In healthy young skin, the dermis forms a type III collagen-rich dermal papilla, where capillary vessels supply oxygen and nutrients to basal epidermal cells. Chicken eggshell membranes (ESMs) have been used as traditional medicines to promote skin wound healing in Asian countries for many years. Previously, we designed an experimental system in which human dermal fibroblasts (HDFs) were cultured on a dish with a solubilized ESM (S-ESM) bound to an artificial phosphorylcholine polymer; we found that genes that promoted the health of the papillary dermis, such as those encoding type III collagen, were induced in the S-ESM environment. The present study found that a gel with a ratio of 20% type III/80% type I collagen, similar to that of the baby skin, resulted in a higher elasticity than 100% type I collagen (p < 0.05) and that HDFs in the gel showed high mitochondrial activity. Thus, we decided to perform further evaluations to identify the effects of S-ESM on gene expression in the skin of hairless mice and found a significant increase of type III collagen in S-ESM. Picrosirius Red staining showed that type III collagen significantly increased in the papillary dermis after S-ESM treatment. Moreover, S-ESM application significantly improved human arm elasticity and reduced facial wrinkles. ESMs may have applications in extending lifespan by reducing the loss of tissue elasticity through the increase of type III collagen.

Recycling different eggshell membranes for lithium-ion battery

In this work, several eggshell membranes and their properties as membrane separators for lithium-ion batteries are investigated. The crystalline phases, morphologies and electrochemical properties of eggshell membranes are characterized by XRD, FE-SEM, CV, EIS, and charge/discharge test. As a result, among eggshell membranes, the chicken eggshell membrane shows the higher amount of protein fiber with the smaller surface area. The battery assembled with chicken eggshell membrane indicates the substitutive option for the traditional separator with the capacity of 90 and 87 mAh g−1 at 1st and 150th cycles, respectively.