Chicken Eggs Research Articles

Research Note: Comprehensive proteomic, phosphoproteomic, and N-glycoproteomic analysis of chicken egg yolk plasma

Chicken egg yolk plasma (EYP), the supernatant fraction of egg yolk obtained by water dilution and centrifugation, is a rich source of various bioactive substances and a significant bearer of yolk-emulsifying properties. This study utilized proteomics to conduct a comprehensive and in-depth analysis of both common and modified EYP proteins (phosphorylated proteins and N-glycosylated proteins). Total of 208 proteins were identified in EYP, including 42 phosphorylated proteins with 137 phosphorylation sites and 150 N-glycoproteins with 332 N-glycosylation sites. Among the phosphorylation sites, tyrosine accounted for 80.6%, while the N-glycosylation sites predominantly featured "N-X-T" motifs, accounting for 58.7%. Functional enrichment analysis revealed that most proteins were involved in regulating enzyme activity and inhibition with a particular focus on modulating peptidase activity. Notably, vitellogenins-2 (30 phosphorylation sites, 9 N-glycosylation sites) and apolipoprotein B (10 phosphorylation sites, 56 N-glycosylation sites) were the 2 proteins with the most modification sites. Additionally, EYP was found to contain the highly N-glycosylated complement proteins C3 and C4. These findings provide new insights into the protein composition of EYP and its roles in chicken embryo development and immune defense, offering a theoretical foundation for the application of EYP in various fields.

EFFECTS OF SPRAYING CEMELE PEPPER (Capsicum annuum L.) EXTRACTS ONTO HATCHING EGGS OF ATAK-S LAYER HENS ON HATCHING RESULTS, SERUM GLUTATHIONE AND MALONDIALDEHYDE LEVELS

Alternative natural products have been used in hatching since chemicals used in the incubation of chicken eggs have toxic effects on the embryo, the practitioners and the environment. In this study with completely randomized design (CRD) the effects of a registered local pepper (Cemele) extract at different dosages in the incubation process of hatching eggs was examined. The chloride group disinfectant (T1), ethanol (T2), 2% pepper extract (T3), and 4% pepper extract (T4) were sprayed on to disinfect eggs before the incubation process. On the second, eighth, and eighteenth incubation days of the groups, the total mesophilic aerobic bacteria (TMAB) loads of the eggshell were examined, and the lowest load values were found at T4. In this study, the highest hatching efficiency (H) and the highest fertilised hatching rate (HF) were 87.52±2.88% and 81.66±1.44%, respectively, in T4. Early embryonic mortality (EEM) was not observed at T4, while the highest value was 7.11±1.35% at T2. The highest mid embryonic mortality (MEM) rate was 10.91±0.34% in T3 while the lowest rate was 7.11±1.35% in T4. The late embryonic mortality (LEM) rates were 14.22±2.69% in T2, 12.47±1.17% in T1, 12.66±2.69% in T3 and 5.30±0.17% in T4. The lowest malposition rate 8.92±0.01% and the lowest malformation rate 8.83±0.12% were observed in T4. At the end of this study, body weights, lengths, tona, and pasgar scores of the hatched chicks were calculated. The highest body weight and length values were observed as 43.57±1.37 g and 16.47±0.45 cm in T4 birds, respectively. The highest tona score was 98.10±1.27 in T4 and the lowest tona score was 93.65±1.22 in T1 birds. Pasgar score values of the chicks were 9.69±0.16 in T4, 9.38±0.17 in T2, 9.31±0.18 at T3 and 9.16±0.16 in T1, respectively. The MDA values of birds were 8.63±0.05, 0.70±0.10, 0.28±0.01 and 0.25±0.01 nmol mL-1 in T1, T2, T3 and T4, respectively while the highest GSH value was 32.39±0.66 M in T4, and the lowest value 11.15±1.03M in T2. In conclusion, the use of Cemele pepper extract had positive results in the incubation process of hatching eggs. Key Words: Incubation process, disinfectant, chlorine dioxide, ethanol, cemele pepper.

PENGARUH KETERJANGKAUAN HARGA, KUALITAS PRODUK, LOKASI, DAN PROMOSI TERHADAP KEPUTUSAN PEMBELIAN

The purpose of this research is to find out how price affordability, product quality, location and promotion influence the decision to purchase Calvin Farm duck eggs. Data processing was carried out using the SPSS 25 program and the sample in this study was obtained using the Slovin formula, which resulted in 100 respondents. Respondents in this research were consumers of Calvin Farm duck eggs. Based on the results of the analysis and discussion in the research that has been carried out, namely based on partial tests, it is stated that Price Affordability (X1), Product Quality (X2), Location (X3), and Promotion (X4) have a significant positive influence on Egg Purchase Decisions (Y). Calvin Farm ducks. Based on the simultaneous test, it is stated that price affordability, product quality, location and promotion together have a significant positive effect on the decision to purchase Calvin Farm duck eggs.

Optimization of Drum Drying Parameters for Chicken Egg White Flour Using Response Surface Methodology

Optimization of Drum Drying Parameters for Chicken Egg White Flour Using Response Surface Methodology

Detection of Antibiotic Resistance Genes in Escherichia coli Isolated from Chicken Eggs

Total 150 chicken eggs were randomly collected from farms, households, and markets of Rajshahi and Naogaon districts. Isolation and identification of E. coli was done by using cultural and staining characteristics, and biochemical properties. Antibiotic sensitivity assay was also performed for the isolated E. coli by using disc diffusion method. Characterization and detection of antibiotic resistance genes in E. coli isolates were done through PCR analysis. Prevalence of E. coli was 30.00% in chicken eggs in Rajshahi and Naogaon districts. Results of antibiotic sensitivity patterns of the isolated E. coli showed 57.78%, 33.33%, 31.11%, 28.89%, 26.67%, 17.78%, 15.56%, 13.33%, 13.33%, and 11.11% resistance to ciprofloxacin, doxycycline, oxytetracycline, ceftriaxone, amoxycillin, erythromycin, levofloxacin, enrofloxacin, gentamycin, and neomycin, respectively. The isolates also showed 94.44%, 82.22%, 80.00%, 77.78%, 66.67%, 60.00%, 55.56%, 48.89%, 46.67%, and 33.33% sensitive to neomycin, enrofloxacin, erythromycin, levofloxacin, gentamycin, ceftriaxone, oxytetracycline, doxycycline, amoxicillin, and ciprofloxacin, respectively. All 45 phenotypic identified isolates were confirmed as E. coli by PCR analysis. Genotypic identified isolates of E. coli were further characterized by PCR to detect antibiotic resistance genes. Out of 45 E. coli isolates 37 (82.22%) showed tetracycline resistance (tetA) gene, 32 (71.11%) showed tetracycline resistance (tetB) gene, and interestingly all 45 (100%) isolates showed quinolone group resistance (gyrA) gene by PCR analysis. J. of Sci. and Tech. Res. 5(1): 135-142, 2023

Contribution of veterinary sector to antimicrobial resistance in One Health compendium: an insight from available Indian evidence.

The application of antibiotics in the poultry and veterinary sectors is very common practice in India. Owing to the seriousness of antimicrobial resistance (AMR), the present study has illustrated the overall scenario of AMR in the poultry and veterinary sectors in India through an in-depth scoping review and key informant interview (KII). In the poultry sector, most of the studies reviewed have reported resistant bacteria isolated from chicken meat, eggs, cloacal swabs, and fecal samples, and only a few have reported the presence of resistant bacteria in and around the environment of poultry farms. The major resistant bacteria that have been reported are E. coli, Salmonella spp., S. aureus, Campylobacter jejuni, and K. pneumoniae. These bacterial isolates exhibited resistance to various antibiotics, such as azithromycin (21.43%), tetracycline (11.30-100%), chloramphenicol (4.76-100%), erythromycin (75-83.33%), ciprofloxacin (5.7-100%), gentamicin (17-100%), amikacin (4.76%), cotrimoxazole (42.2-60%), trimethoprim (89.4%), ceftriaxone (80%), and cefotaxime (14.29-70%). Like the poultry sector, different antibiotics are also used for treating clinical and subclinical bovine mastitis, which is one of the major problems plaguing the dairy sector. Several AMR bacterial strains, such as E. coli, Staphylococcus aureus, S. epidermidis, and Klebsiella pneumoniae, have been reported by many researchers and showed resistance against tetracycline (74%), oxytetracycline (47.37%), ciprofloxacin (51%), streptomycin (57.89%), cephalosporin (100%), and trimethoprim (70%). The KIIs have revealed several reasons behind these AMR scenarios, of which the growing need for the production of food animals and their products with inadequate infrastructure and a lack of proper knowledge on farm management among the farmers are the major ones. Though several government legislations and policies have been laid down, proper implementation of these policies, strict surveillance on antibiotic application in the poultry and veterinary sectors, awareness generation among farmers, and infrastructure development can help minimize the development and transmission of AMR bacteria within and from these sectors.

Influence of Corona Virus Disease (COVID-19) on Consumption and Purchasing Patterns of Poultry Products from Three Selected Districts in Sri Lanka

The study reported herein investigated the effects of COVID-19 pandemic on purchasing and consumption patterns of poultry products among the communities in Kandy, Gampaha and Galle districts of Sri Lanka. A pre-tested questionnaire was used to collect data from 629 respondents. The questionnaire explored different socio-economic and health factors affecting on consumption and purchasing frequencies of chicken meat, meat products and eggs before and during the pandemic. The results revealed that the pandemic has made an impact on consumption frequencies of chicken or processed chicken products. Weekly purchasing amount of chicken or processed chicken products has been declined during the pandemic. Many respondents increased their egg consumption during the pandemic. Stocking poultry products during the pandemic has also increased. The study concluded that though the purchasing frequencies and the amount of chicken or processed chicken products have been changed consequently to the pandemic, neither the purchasing method nor purchasing brand has been changed. The pandemic had no influence on buying patterns for eggs but has contributed to increased egg consumption frequency among the public.

In-Ovo Antiviral Activity of Hibiscus sabdariffa against Newcastle Disease Virus

Newcastle disease is a highly contagious viral infection affecting poultry and wild birds. The causative agent is Avian paramyxovirus 1 (APMV-1), causing significant economic losses despite vaccination efforts due to its high mortality rate. Hibiscus sabdariffa was identified at Modibbo Adama University Yola, and laboratory assays were performed at the National Veterinary Research Institute Vom. The study explores the antiviral effects of extracts from H. sabdariffa calyx against a virulent strain of Newcastle Disease Virus (NDV) using embryonated chicken eggs (ECE). Standard methods were employed for cytotoxicity assay, embryo infective dose 50 (EID50) determination, and therapeutic antiviral assays. Methanol was used for extraction and phytochemical analysis, revealing various bioactive compounds like cardiac glycosides, alkaloids, flavonoids, terpenes, and phenols. Toxicity assay showed cytotoxicity at concentrations over 25 mg/ml, but therapeutic antiviral assays demonstrated virus replication inhibition at concentrations as low as 5 mg/ml. These findings suggest the potential of H. sabdariffa calyx extracts as safe and effective treatments for NDV, with promising therapeutic antiviral properties. Further pharmaceutical research is recommended to explore their use in developing novel Newcastle Disease treatments.

Assessment of the presence of multidrug-resistant Escherichiacoli, Salmonella and Staphylococcus in chicken meat, eggs and faeces in Mymensingh division of Bangladesh

The emergence of bacteria that is resistant to several drugs of clinical importance poses a threat to successful treatment, a phenomenon known as multidrug resistance that affects diverse classes of antibiotics. The purpose of this study was to evaluate the prevalence of multidrug-resistant Escherichiacoli, Salmonella spp. and Staphylococcusaureus in chicken egg, meat and faeces from four districts of Bangladesh. A total of 120 chicken samples were collected from different poultry farms. Conventional culture and molecular detection methods were used for identification of bacterial isolates from the collected samples followed by antibiotic susceptibility test through the disc diffusion method, finally antibiotic resistant genes were detected by PCR. E. coli, Salmonella spp. and Staphylococcusaureus were detected in meat, egg and faecal samples. Antimicrobial susceptibility results revealed isolates from faeces were 100 % resistant to amoxicillin, while all S.aureus and Salmonella sp. from faeces were resistant to doxycycline, tetracycline and erythromycin. Salmonella spp. isolates from eggs indicated 100 % resistance to erythromycin, amoxycillin, while E.coli were 100 % resistant to erythromycin. E.coli and S.aureus from meat were 100 % resistant to amoxicillin and erythromycin. However, Salmonella spp. from eggs were 100 % susceptible to doxycycline, gentamicin, levofloxacin and tetracycline. The mecA and aac(3)-IV genes were only found in S.aureus and E.coli, respectively. The Sul1,tetB, and aadA1 were highest in Salmonella spp. and S.aureus, while the sul1,tetA and blaSHV were higher in E.coli. Isolates from all samples were multidrug resistant. These findings indicate a high risk of transmission of resistance genes from microbial contamination to food of animal origin. The study emphasizes the need for effective biosecurity measures, responsible antibiotic use, and strict regulations in poultry production to prevent the spread of antibiotic resistance.

Changes of Potent Odorants in Salted Duck Egg Yolk before and after Roasting.

As the second most widely consumed eggs, duck eggs are made into preserved eggs, salted duck eggs, and roasted duck eggs to extend their shelf-life. To investigate the differences in potent odorants (POs) between salted duck egg yolk (SDEY) and roasted duck egg yolk (RDEY), the volatiles in SDEY and RDEY were extracted through solvent extraction coupled with solvent-assisted flavor evaporation and were assayed with gas chromatography-mass spectrometry-olfactometry. A total of 45 volatiles were identified in two samples, 24 odor-active compounds (OACs) were screened, and more OACs were in RDEY. The flavor-dilution (FD) factors of OACs were obtained by aroma extract dilution analysis and ranged from 3 to 6561. Twenty-two OACs with FD factors ≥ 9 were quantitated, and the results indicated the concentrations of OACs in yolk increased greatly after salted duck eggs were roasted. Based on the concentrations and thresholds, odor activity values (OAVs) were determined; 17 odorants with OAVs ≥ 1 were determined as POs. Acetoin was the most PO in SDEY; there were more POs in RDEY, including 2-ethyl-3,6-dimethylpyrazine, acetoin, 2-acetyl-3-methylthiophene, dihydro-4-hydroxy-2(3H)-furanone, etc. The outcomes obtained have reference values for making better use of duck eggs in the food industry.

Decision Making on The Organoleptic Quality of Salted Egg Products using Analytical Hierarchy Process and Simple Additive Weighting

Chicken and duck eggs are highly nutritious food products that are popular among the Indonesian community. However, their perishable nature leads to a decline in quality if not further processed. The salting process is a simple preservation method that can also enhance the flavor of salted egg products. To produce salted egg products with high organoleptic quality preference, a combination of Analytical Hierarchy Process (AHP) and Simple Additive Weighting (SAW) is implemented as a quality decision-making method based on: (1) the experts viewpoints on the importance level of organoleptic criteria, and (2) the scores of organoleptic tests for the criteria of texture, taste, and appearance. In this study, chicken and duck eggs were processed into salted eggs using a salting mixture made of a blend of scouring ash and table salt with a mass ratio of 5:4 and 5:6, resulting in product combinations coded as CE-5/4, CE-5/6, DE-5/4, and DE-5/6. Data processing results on the importance level from the experts viewpoints using the AHP method resulted that the criteria for texture, taste, and aroma had final priority weights of 0.244, 0.617, and 0.139, respectively. Final decision-making using the SAW method indicated that the highest to lowest final preference scores were obtained by products CE-5/4, DE-5/4, DE-5/6, and CE-5/6, with the final preference scores of 0.979, 0.963, 0.931, and 0.906, respectively. The results demonstrated that AHP and SAW were successfully implemented to assist in making decisions regarding the quality of salted egg products based on their organoleptic characteristics. Keywords: salted egg, organoleptic, decision-making, AHP, SAW

Effects of mulberry leaf enrichment with Lepidium sativum L. seed powder suspension on the economic parameters of Bombyx mori L

The phytochemicals of high nutritional and functional properties in Lepidium sativum L. (garden cress) seeds have nominated their seed powder (regardless of the concentration used) for enrichment of mulberry leaves in order to enhance Bombyx mori L. larval feeding, and consequently to gain ground in sericulture industry. As expected, B. mori larval feeding on L. sativum-enriched mulberry leaves showed not only a remarkable increase in mean values of certain economic parameters of B. mori, such as cocoon weight, cocoon shell weight, pupal weight, and egg yield, compared with the control group, but also showed a phenomenal increase in egg counts (on average, ca. 958–1256 eggs laid per female moth) and a significant increase in egg size (measured as egg surface area and egg volume). Male or female moth larval diet has significantly influenced the reproductive performance or fitness of both sexes of B. mori in terms of large-sized moths (measured as forewing, hind femur, and hind tibia lengths) and highly fecund moths (i.e., increased fecundity and spermatophore counts per female moth, and large-sized eggs). On the basis of B. mori female moth reproductive index, the female moths from L. sativum-fed larvae proved to have a lower reproductive index compared to their corresponding value for females of the control group, indicating more efficient utilization of larval resources for B. mori reproduction. Quantification of the three main physiological resources viz., protein, lipid and carbohydrate in the internal reproductive tract of B. mori female moths at death has nominated the female moth abdomens, or simply their bodies, as being a reasonable natural source of protein, lipid, and carbohydrate, to be involved in certain manufactures (e.g., pet feed formulations) instead of discarding them as a source of environmental pollution. Evidently, the L. sativum seed powder is of considerable interest because it remarkably improves the performance of such an economically important insect, B. mori. This is the first study for evaluating the efficacy of L. sativum seed powder in sericulture field to enhance B. mori productivity parameters.

Exploring Cross-Reactivity Among Hen, Duck, and Quail Eggs in Children with Hen’s Egg Allergy

Exploring Cross-Reactivity Among Hen, Duck, and Quail Eggs in Children with Hen’s Egg Allergy

In vitro analysis of antiviral immune response against avian influenza virus in chicken tracheal epithelial cells.

Avian influenza virus (AIV) infections first affect the respiratory tract of chickens. The epithelial cells activate the host immune system, which leads to the induction of immune-related genes and the production of antiviral molecules against external environmental pathogens. In this study, we used chicken tracheal epithelial cells (TECs) in vitro model to investigate the immune response of the chicken respiratory tract against avian respiratory virus infections. Eighteen-day-old embryonic chicken eggs were used to culture the primary chicken TECs. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC) analysis of epithelial cell-specific gene makers were performed to confirm the characteristics, morphology, and growth pattern of primary cultured chicken TECs. Moreover, to investigate the cellular immune response to AIV infection or polyinosinic-polycytidylic acid (poly (I:C)) treatment, the TECs were infected with the H5N1 virus or poly (I:C). Then, immune responses were validated by RT-qPCR and western blotting. The TECs exhibited polygonal morphology and formed colony-type cell clusters. The RT-qPCR results showed that H5N1 infection induced a significant expression of antiviral genes in TECs. We found that TECs treated with poly (I:C) and exposed to AIV infection-mediated activation of signaling pathways, leading to the production of antiviral molecules (e.g., pro-inflammatory cytokines and chemokines), were damaged due to the loss of junction proteins. We observed the activation of the nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways, which are involved in inflammatory response by modulating the release of pro-inflammatory cytokines and chemokines in TECs treated with poly (I:C) and pathway inhibitors. Furthermore, our findings indicated that poly (I:C) treatment compromises the epithelial cell barrier by affecting junction proteins in the cell membrane. Our study highlights the utility of in vitro TEC models for unraveling the mechanisms of viral infection and understanding host immune responses in the chicken respiratory tract.

Enhancing Selectivity with Molecularly Imprinted Polymers via Non-Thermal Dielectric Barrier Discharge Plasma.

Molecularly imprinted polymers (MIPs) are synthetic polymers that mimic the functions of antibodies. Though MIPs are promising tools in various areas, achieving high selectivity in MIPs can be difficult. To improve selectivity, various approaches have been implemented; however, the role of polymerization methods or synthetic techniques in enhancing the selectivity of MIPs has not been studied and remains a crucial area for further research. MIPs are typically prepared from free radical reactions. Recently, we found that Dielectric Barrier Discharge (DBD) plasma can be used to initiate the polymerization of vinyl monomers. The DBD plasma method allows the monomers to associate with the template molecules and initiate polymerization with minimal disruption to the positioning of the monomers. We hypothesize that this could be a preferred method to prepare MIPs over the traditional radical reaction that may cause a disturbance of the pre-associated monomers on the templates for the polymerization. Chicken egg white serum albumin (CESA) was used as the template protein for the MIPs. Our results show that in all test conditions, approximately twofold improvement in selectivity was achieved, which is the primary performance metric for MIPs. This enhancement was evident across all categories, including MIPs prepared from various monomer combinations.

Inactivation of Highly Pathogenic Avian Influenza Virus with High-temperature Short Time Continuous Flow Pasteurization and Virus Detection in Bulk Milk Tanks

Infections of dairy cattle with clade 2.3.4.4b H5N1 highly pathogenic avian influenza virus (HPAIV) were reported in March 2024 in the U.S. and viable virus was detected at high levels in raw milk from infected cows. This study aimed to determine the potential quantities of infectious HPAIV in raw milk in affected states where herds were confirmed positive by USDA for HPAIV (and therefore were not representative of the entire population), and to confirm that the commonly used continuous flow pasteurization using the FDA approved 72 °C (161°F) for 15 s conditions for high−temperature short time (HTST) processing, will inactivate the virus. Double-blinded raw milk samples from bulk storage tanks from farms (n = 275) were collected in four affected states. Samples were screened for influenza A using quantitative real-time RT-PCR (qrRT-PCR) of which 158 (57.5%) were positive and were subsequently quantified in embryonating chicken eggs. Thirty-nine qrRT-PCR positive samples (24.8%) were positive for infectious virus with a median titer of 3.5 log10 50% egg infectious doses (EID50) per mL. To closely simulate commercial milk pasteurization processing systems, a pilot-scale continuous flow pasteurizer was used to evaluate HPAIV inactivation in artificially contaminated raw milk using the most common legal conditions in the US: 72 °C (161°F) for 15 s. Among all replicates at two flow rates (n = 5 at 0.5 L/min; n = 4 at 1 L/min), no viable virus was detected. A mean reduction of ≥5.8 ± 0.2 log10 EID50/mL occurred during the heating phase where the milk is brought to 72.5 °C before the holding tube. Estimates from heat-transfer analysis support that standard U.S. continuous flow HTST pasteurization parameters will inactivate >12 log10 EID50/mL of HPAIV, which is ∼9 log10 EID50/mL greater than the median quantity of infectious virus detected in raw milk from bulk storage tank samples. These findings demonstrate that the US milk supply is safe when pasteurized.

Quantitative metabolomic analysis of yolk granules from different poultry eggs

A systematic comparison of the nutrient composition of yolk granules from different poultry egg sources may provide a reference for the development of nutritionally balanced foods with high protein, low fat, and low cholesterol content. The metabolomic profiles of chicken, duck, and quail egg yolk granules were analysed quantitatively using UPLC-MS/MS. A total of 820 metabolites were identified, with amino acids and their metabolites (232), glycerophospholipids (121), and organic acids and their derivatives (96), representing the three most abundant metabolite classes in egg yolk granules. The quantitative results demonstrated that amino acids and their metabolites, as well as glycerophospholipids (GP), exhibited significant differences among the three egg yolk granules. A total of 13 coenzymes and vitamins were identified in the three egg yolk granules, with the majority belonging to the B vitamins and their derivatives. A total of 11 bile acids were identified in the three egg yolk granules, including four primary and seven secondary bile acids. A total of 20 significantly different metabolites were preliminarily identified as potential markers for the differentiation of poultry egg yolk granules. This information provides crucial insights into the nutritional composition of egg yolk granules from diverse sources.

Experimental immunotherapy of association infections of the gastrointestinal tract of newborn calves using yolk melange enriched with specific Y class immunoglobulins

Mass gastrointestinal diseases of newborn calves manifested by symptoms of diarrhea are caused by various etiological factors and most often occur in the form of mixed infections. Mixed infections have a more severe diarrhea than mono-infections. The economic consequences of such infections due to the high prevalence, frequent mortality and loss of productivity during further exploitation of the animal can be significant. The most common causative agents of gastrointestinal diseases of newborn calves of viral etiology is rotavirus and coronavirus infections. Mixed (viral-bacterial) infection can lead to 100% mortality of the diseased calves. Specific vaccination of springer cows against gastrointestinal pathogens in calves, which is performed to increase the number of pathogen-specific immunoglobulins in the colostrum at calf birth, and ingestion of colostrum by the calves during the first 4 hours of life are the most important strategies to prevent severe diarrhea. But as practice shows, this is not always enough. The purpose of this work was to study the effectiveness of immunoprophylaxis of associative infections of the gastrointestinal tract of newborn calves with the use of melange prepared from chicken eggs previously immunized with the vaccine "Rotagal" (Pharmagal-BIO s.r.o., Slovakia), used for the prevention of colibacillosis, rotavirus and coronavirus infections of cattle. As a result of the study, it was established that the addition of yolk melange rich in specific immunoglobulins of Y class to the colostrum in the first hours of life of the calves has a pronounced prophylactic effect, facilitates the course of the infectious process in case of the disease, saves costs for the treatment of sick animals, increases survival rate, contributes to the acceleration of live weight gain. The use of an immunobiological preparation based on transovarial immunoglobulins has a positive effect on the indicators of nonspecific resistance of newborn calves compared to the calves with early postnatal disease and antibiotic treatment.

Increased nanosphere size in the cuticle layer of Japanese quail egg by mutation in the myostatin gene

Cuticle quality can affect food safety by protecting poultry eggs from bacterial infection in the modern poultry industry. However, genetic factors related to cuticle nanostructure are not much reported due to limited bird models. In the current study, the genome-edited quail targeting myostatin (MSTN) gene was used to investigate the effect of MSTN mutation on the cuticle nanostructure and quality. To analyze nanostructure of the cuticle layer of the MSTN mutant and wild-type (WT) quail eggs, scanning electron microscope (SEM) images was taken. Thickness of the cuticle layer did not differ between the MSTN mutant and WT groups, but the size of the nanospheres in the surface of the cuticle layer was increased by MSTN mutation. In addition, increased size of the nanospheres in the MSTN mutant group was also shown in the upper region of the cross-sectional cuticle layer. Notably, both groups showed similar small-sized nanospheres in the lower region of the cuticle layer and the size was increased as they ascended to the upper region. The data suggested that MSTN mutation increased the size of the nanosphere in the upper region of the cuticle layer at a late phase rather than increasing the size of nanospheres in the lower region of the cuticle layer at an early phase of cuticle formation. However, the number of Escherichia coli attached to the surface did not differ between the two groups indicating no association between nanosphere size and bacterial attachment in quail eggs. The current study demonstrated a new function of the MSTN gene on regulation of cuticle nanostructure, for the first time. These results advanced our knowledge on the association between genetic factors and cuticle nanostructure and can be served as a reference to study the mechanism of cuticle formation in the future study.

Kualitas internal telur ayam ras yang direndam larutan daun kelor dengan lama penyimpanan selama 21 hari

This study aims to determine the quality of broiler chicken eggs soaked in Moringa leaf solution. This research was conducted by immersing purebred chicken eggs in Moringa leaf solution for 1 day and using a Completely Randomized Design (CRD) method with a sample of 80 purebred chicken eggs consisting of 4 treatments where each treatment consisted of 4 replications and each replication consists of 5 samples. Each treatment of the experimental design used in this study was P0 (without treatment), P1 (addition of 20% Moringa leaf solution), P2 (addition of 30% Moringa leaf solution), P3 (addition of 40% Moringa leaf solution). The variables observed were egg Kuningindex, egg white index and air voids.The results of the study showed that soaking chicken eggs in Moringa leaf solution at P3 (soaking in 40% Moringa leaf solution) had a real effect on the egg white index. The results of the extension evaluation carried out in the Pesona Mutiara Farmer Women's Group,