Chicken Eggs Research Articles

Effect of the LIF gene on the cell cycle and apoptosis of ovarian granulosa cells in white Muscovy ducks

Leukaemia inhibitory factor (LIF), a member of the interleukin-6 (IL-6) family, is a multifunctional cytokine. The maturation-to-ovulation process of poultry follicles is determined by granulosa cell proliferation and differentiation. Granulosa cell apoptosis and degeneration lead to follicular atresia, which reduces the number of normally developing follicles and leads to a decrease in the poultry egg production rate, thus affecting the large-scale development of poultry breeding. In this study, the LIF gene overexpression vector pCDH-CMV-LIF and a siRNA that inhibits LIF gene expression were transfected into primary granulosa cells from white Muscovy duck ovaries for functional study. Compared with that in the control group, LIF gene expression was confirmed to be significantly decreased or increased in the transfection groups (P < 0.01). After LIF overexpression, the expression of the cell cycle-related genes CCND1, CDK-1 and PCNA was decreased (P < 0.05); apoptosis was promoted; the proapoptotic genes Bax and caspase-3 were significantly upregulated (P < 0.01); and the antiapoptotic gene Bcl-2 was significantly downregulated (P < 0.01). After LIF interference, the expression of the cell cycle-related genes CCND1, CCNE1, CDK-1 and PCNA and the antiapoptotic gene Bcl-2 significantly increased (P < 0.01), whereas the expression of the proapoptotic genes Bax, caspase-3 and caspase-9 significantly decreased (P < 0.01). In summary, the LIF gene is involved in regulating the biological function of ovarian granulosa cells in white Muscovy ducks. LIF gene expression promotes granulosa cell apoptosis and inhibits cell cycle progression. These experimental results provide insights into the follicular development mechanism of white Muscovy ducks.

Assessment of genotoxic potential of fragrance materials in the chicken egg assays.

The genotoxic and clastogenic/aneugeneic potentials of four α,β-unsaturated aldehydes, 2-phenyl-2-butenal, nona-2-trans-6-cis-dienal, 2-methyl-2-pentenal, and p-methoxy cinnamaldehyde, which are used as fragrance materials, were assessed using the Chicken Egg Genotoxicity Assay (CEGA) and the Hen's egg micronucleus (HET-MN) assay, respectively. Selection of materials was based on their chemical structures and the results of their previous assessment in the regulatory in vitro and/or in vivo genotoxicity test battery. Three tested materials, 2-phenyl-2-butenal, nona-2-trans-6-cis-dienal, and 2-methyl-2-pentenal, were negative in both, CEGA and HET-MN assays. These findings were congruent with the results of regulatory in vivo genotoxicity assays. In contrast, p-methoxy cinnamaldehyde, which was also negative in the in vivo genotoxicity assays, produced evidence of DNA damage, including DNA strand breaks and DNA adducts in CEGA. However, no increase in the micronucleus formation in blood was reported in the HET-MN study. Such variation in responses between the CEGA and HET-MN assay can be attributed to differences in the dosing protocols. Pretreatment with a glutathione precursor, N-acetyl cysteine, negated positive outcomes produced by p-methoxy cinnamaldehyde in CEGA, indicating that difference in response observed in the chicken egg and rodent models can be attributed to rapid glutathione depletion. Overall, our findings support the conclusion that CEGA and/or HET-MN can be considered as a potential alternative to animal testing as follow-up strategies for assessment of genotoxic potential of fragrance materials with evidence of genotoxicity in vitro.

Comprehensive risk assessment of lead concentrations in chicken, quail, and duck egg albumen and yolk using Monte Carlo simulations

This study conducted a comparative analysis of the concentration of the lead (Pb) in the albumen and yolk of eggs from domesticated chicken, quail, and duck, with a concurrent assessment of the potential carcinogenic and non-carcinogenic risks associated with the consumption of eggs sourced from Türkiye. A total of 78 poultry egg samples were gathered from breeding farms and farmers' markets situated in Şanlıurfa province. Lead concentrations were assessed through inductively coupled plasma optical emission spectrometry (ICP-OES). Human health risk assessment adheres to the guidelines set forth by the United States Environmental Protection Agency (US EPA), which primarily emphasizes estimated daily intake (EDI), international lifetime cancer risk (ILCR), target hazard quotient (THQ), and Monte Carlo simulation (MCS) as a probabilistic approach. One-way analysis of variance (ANOVA) was employed to compare Pb concentrations within egg yolks and albumens, as well as among various types of eggs. The levels of Pb found in the albumen of chicken, quail, and duck eggs were measured to be 0.31 ± 0.11, 0.43 ± 0.11, and 0.47 ± 0.16 μg kg−1, respectively. The concentrations of Pb in the yolks of chicken, quail, and duck eggs were found to be 0.54 ± 0.19, 0.28 ± 0.11, and 0.69 ± 0.21 μg kg−1, respectively. These concentrations were below the maximum permitted levels set by the FAO/WHO. The results indicated that Pb content in all tested eggs was safe for consumption, with exposure levels significantly below Joint FAO/WHO Expert Committee on Food Additives (JECFA) risk thresholds. The THQ values were less than one, indicating no non-carcinogenic risk. In addition, this study provides accurate and reliable data for policy makers to improve food safety measures and reduce potential public health risks.

Design: UI/UX Penjualan Telur Ayam di Cilacap dengan Metode R&amp;D

Nowadays, more and more e-commerce platforms are emerging, creating a highly competitive environment for both individuals and groups. User experience and interface are key factors in attracting users' attention and fulfilling various needs to survive in a crowded market. In this context, the author proposes the prototyping of a chicken egg sales application named EiTech Supply in Cilacap Regency. This application is designed to overcome the problems that chicken egg farmers often face, such as competition between regions and the entry of new farmers with very low prices to attract customers. With the EiTech Supply application, it is expected to reduce these problems and increase the competitiveness of chicken egg farmers both inside and outside the region.

BENEFITS OF TEAS AND PROBIOTIC PROFILE AT SPECIFIC STAGES OF EMBRYONIC DEVELOPMEN IN CHICKEN EGGS

Introduction: The Sharr Mountain is characterized by a highly expressed biological diversity, with a particular endemism and a wide spectrum of various types of flora characteristic of this mountainous massif. Plants with nutritional and health benefits can be considered species such as: mountain tea (Sideritis scardica) and St. John's wort (Hypericum perforatum), etc. These bioproducts are characterized by special importance both in terms of their presence, quality, and health benefits. Therefore, exploration of these species would have positive cost for consumption as forms of teas or plant extracts for commercial purposes, but also for health benefits and above all for the population of the country, the region, and beyond. Research Objective: This study aims to investigate the probiotics and the effect of plant extract teas such as mountain tea (Sideritis scardica) and St. John's wort (Hypericum perforatum) by applying a specific dose of extract to chicken embryos at different stages of ontogenesis. Materials and Methods: Fertilized chicken eggs were taken for the study and were incubated in an incubator for 21 days. The eggs were treated with species such as mountain tea (Sideritis scardica) and St. John's wort (Hypericum perforatum) at certain stages of embryonic development. The eggs were opened on the seventh, fourteenth, and twenty-first day. Macroscopic and microscopic changes at certain stages of embryonic development were analyzed using stereomicroscope.The effect of the extract of these teas was studied on the chicken embryos (Gallus domesticus), by applying a specific dose of extract to the chicken embryos at different stages of embryonic development during ontogenesis. Research Results: The dose given in the form of extract has resulted in positive benefits of these teas as effective probiotics during certain stages of embryonic development in chicken embryos. Conclusions: This research will explore the effect of the given dose of plant extracts at certain stages of embryonic development during ontogenesis to observe the benefits of dose administration at specific quantities and times to detect the positive effect of these teas.

MONITORING OF THE LIVING ENVIRONMENT THROUGH AIR POLLUTANTS IN THE POLOG FIELD OF THE REPUBLIC OF NORTH MACEDONIA

Introduction: The Republic of North Macedonia and especially the field pit of Polog in terms of geographical position and climatic aspect is considered as a place with different variables such as: temperature, humidity, radioactive exposure, hilly-mountainous relief. The Sharr mountain in the west and the dry mountain in the north, limit the Polog field pit in all directions, such as north-west and south-east, causing the living environment to deteriorate in the winter period due to air, water and land pollutants. With special emphasis, we highlight the winter season when we have an alarming state of air pollution, such as certain regions of the Republic of North Macedonia and especially the region of the field pit of Polog with the cities of Tetovo and Gostivar and the surrounding area. Recently, the city of Tetova and its surroundings is considered one of the most polluted cities in the country, region and the largest in the world, then this phenomenon of pollution necessarily leads us to unwanted consequences for the population that lives and operates in this region. As a result of environmental factors such as air pollution and other related factors such as: stress, temperature, food, social genes, mental state and other related factors can lead to disorders in the human body and lead to diseases that attack the nervous and cardiovascular systems , skeletal and thyroid. Many studies talk about the alarming condition of patients who are affected by these diseases, therefore our paper will focus on the effect of these factors above all on the actual state of pollutants in the Polog region of North Macedonia. The purpose of the study: This paper aims to investigate the potential causes that can affect the health of the population of the Polog field pit and especially the city of Tetova and its surroundings. As a potential pollutants of the living environment, the following can be considered: carbon monoxide, carbon dioxide, sulfur dioxide, urban combustion from automobiles, combustion of various physical substances, chemical components from factories, smoke from chimneys from burning oil and coal, combustion from wood in the form of pellets, etc. Material and working method: In the study, fertilized chicken eggs were taken and incubated in the incubator for 21 days of incubation. The eggs were treated with different doses of air pollutant extract at certain stages of embryonic development. Using the stereomicroscope with macroscopic and microscopic method were analyzed ontogenetic changes in certain stages of embryonic development. Research results: The results of the research will give a real insight into the situation of the level of pollution of the living environment in the city of Tetovo and its surroundings and in the field pit of Pollog. The monitoring of the living environment can be followed by the treatment of air pollutants in certain doses in the form of ampoule therapy, and the negative effect of the given dose on the hen's eggs will be observed. All eggs with the highest dose of injection had a stagnated embryonic development, whereas egss with lower dose of injections had underdeveloped embryos. Conclusion: From the results of the research we can conclude that the potential pollutants from air pollutants can lead to consequences for the population living in this region but also for the living world in general. These factors can lead to disorders of the nervous system, cardiovascular, and thyroid disorders and other respiratory diseases. This research will give a real insight into the pollution of the living environment and the possible consequences of this at a local, regional and wider level.

The effectiveness of biotin in therapy alopecia of various origins, pathologies of the skin and nails

Biotin deficiency is caused by inflammatory bowel diseases that impair the absorption of the vitamin, special dietary disorders with the consumption of raw eggs (excess avidin – a vitamin B7 blocker protein), magnesium deficiency, smoking, alcohol, treatment with broad-spectrum antibiotics, sulfonamides, and anticonvulsants. Hypovitaminosis B7 has also been noted in individuals with congenital genetic defects of the biotinidase gene or other genes involved in biotin metabolism. Deficiency of water-soluble vitamin B7 (vitamin H) – manifested by dry skin, seborrheic dermatitis, dermatitis around the eyes, nose, mouth, ears and groin, impaired nail growth, slow healing of skin cuts, atopic dermatitis, striations, splitting, brittle nails and alopecia (diffuse and androgenic form). Alopecia occurs when hair follicles die and leads to hair loss. The human proteome contains 51 proteins involved in biotin metabolism. In particular, D-biotin-dependent carboxylases play an important role in the metabolism of fatty acids, amino acids, carbohydrates, cell division and growth, incl. keratinocytes and hair follicle cells. The molecular mechanisms of the effects of D-biotin on the skin and its appendages may involve various growth factors: regulation of the signaling pathways of growth factors (IGF-1, FGF, KGF, HGF, VEGF, SIRT-1, Wnt and beta-catenin) has been shown. Hair follicle stem cells cause the cyclical growth of hair follicles. Growth factors are involved in the activation of stem cell growth by D-biotin; activation of the Wnt/β-catenin signaling cascade leads to the activation of cyclin D1 proteins (initiates DNA synthesis and leads to increased viability of hair follicles. The results of fundamental and clinical studies confirm the prospects of using biotin in dermatology for the treatment of diseases of the skin, hair and nails, incl. alopecia of various origins (androgenic, focal, diffuse). The results of the studies indicated that biotin was well tolerated, and there was no risk of hypervitaminosis even when taking megadoses (hundreds of milligrams).

THE EFFECT OF THE ANTIBIOTIC GENTAMICIN ON THE STAGES OF ONTOGENESIS IN CHICKEN EGGS

Introduction: Various studies have shown that the effects of the action of drugs, in addition to having positive effects, they can also provoke negative effects in different organs of the body, where by giving more doses in some cases it can exceed the therapeutic limit and become toxic and produce pathological effects in both experimental animals and humans. Uncontrolled use and drug overdose can lead to macroscopic histopathological changes and give teratogenic, mutagenic and carcinogenic effects. Purpose of the paper: This paper aims to study the effects of some drugs at different stages of embryonic development in chicken eggs. The research of this paper will consist of the toxic effect of drugs and macroscopic and microscopic histopathological changes in certain stages of embryonic development of chicken eggs. Material and method: In the study, fertilized chicken eggs were taken and incubated in the incubator for 21 days of incubation. The eggs were treated with the antibiotic gentamicin at certain stages of embryonic development. The eggs hatched on day thirteen, day fifteen, eighteen and day twentyone. Using the stereomicroscope with the macroscopic and microscopic method were analyzed ontogenetic changes in certain stages of embryonic development. Results: The given dose of the antibiotic gentamicin can have negative effects in certain stages of embryonic development in chicken eggs. The dose given in ampullary form in certain quantities to chicken eggs has inhibited and stagnated the processes of embryonic development at different stages compared to control eggs. Conclusion: The given dose of gentamicin can stagnate embryonic development in certain stages and can provoke changes in certain stages of embryonic development. This phenomenon encourages us to do even more detailed research in this direction to see the effect of the given dose and to analyze what consequences it can provoke overdoses at different stages of embryonic development and within the organism. The lethal dose applied at certain stages of ontogenesis may have lethal consequences for the chick embryo at certain stages of embryonic development.

Efektivitas Rebus Daun Sirih Merah dan Konsumsi Telur Ayam Rebus terhadap Luka Perineum pada Ibu Postpartum di PMB S Kabupaten Pandeglang Tahun 2024

Poor care of perineal wounds can cause infection which will slow down the healing process of perineal wounds. Efforts to prevent perineal wound infections can be provided with pharmacological therapy and non-pharmacological therapy. To provide midwifery care and determine the effectiveness of boiling red betel leaves and consuming boiled chicken eggs on perineal wounds in postpartum mothers. The research used is descriptive qualitative research. The target of the activity was 2 postpartum mothers who had perineal wounds who were given intervention 1 by giving boiled red betel leaves, and intervention 2 by giving boiled chicken eggs for 5 days. From the research results, it was found that postpartum mothers who experienced perineal wounds before and after being given intervention experienced accelerated healing of perineal wounds on the 4th day. There is an effect of boiled red betel leaves and boiled chicken eggs on accelerating the healing of perineal wounds in postpartum mothers. For postpartum mothers, consume boiled chicken eggs and red betel leaves to speed up the healing of perineal wounds.

Comparison of immunoglobulin Y antibody production in new and spent laying hens.

Immunoglobulin (Ig)Y, a specific type of Ig found in chicken eggs, has potential use in the diagnosis of human and animal diseases. This study assessed the feasibility of using spent laying hens to produce IgY. In addition, the effects of antigen injection on egg and antibody production in new and spent laying hens were compared. Hens were intramuscularly injected with three booster shots of antigens. IgY was extracted from egg yolks using polyethylene glycol 6000 precipitation followed by dialysis. Spent laying hens (83 weeks) consistently showed lower egg production than new laying hens (27 weeks) throughout the study. Post-immunization, a further decline in egg production was observed in spent laying hens, and egg production stopped after the second antigen injection. Eggs from spent laying hens were less dense than eggs from new hens. Despite lower IgY levels in eggs from spent laying hens, the heavy-to-light chain ratio remained consistent in both groups. Notably, IgY from spent and new laying hens demonstrated effective hemagglutination against cat erythrocytes in the A blood group. This study demonstrated the potential of using spent laying hens to produce IgY, with significant implications for future research, immunotherapy, and diagnostic applications, despite the observed reduction in egg production compared with new laying hens.

P21-03 Risk assessment of PFAS in home-produced chicken eggs in the Netherlands

P21-03 Risk assessment of PFAS in home-produced chicken eggs in the Netherlands

A comprehensive review on freeze-induced deterioration of frozen egg yolks: Freezing behaviors, gelation mechanisms, and control techniques.

Over the years, the production of eggs has increased tremendously, with an estimated global egg production of 9.7 billion by 2050. Further processing of shell eggs to egg products has gained growing popularity. Liquid egg yolks, an innovative form of egg replacement, still suffer from short shelf-life issues, and freezing has been applied to maintain freshness. An undesirable phenomenon called "gelation" was found during the production of frozen egg yolks, which has attracted numerous scholars to study its mechanism and quality control methods. Therefore, we comprehensively reviewed the history of the studies on frozen egg yolks, including the production procedure, the fundamentals of freezing, the gelation mechanism, the factors affecting gelation behaviors, and the techniques to control the gelation behaviors of frozen egg yolks. Reporting the production procedure and freezing fundamentals of frozen egg yolks will give readers a better understanding of the science and technological aspects of frozen egg yolks. Furthermore, a comprehensive summary of the mechanism of egg yolk gel formation induced by freeze-thawing and relevant control techniques will provide insights to researchers and manufacturers in the field of frozen egg processing.

Comprehensive lipidomics and flavoromics analysis reveals the formation mechanism of the unique flavor in quail egg yolks during thermal treatment

After thermal treatment, quail eggs pruduce an attractive flavor that is favored by consumers. In this study, the key aroma volatiles and their lipid precursors during thermal treatment were investigated by the electronic nose, GC-MS, GC-O-MS and UPLC-Q-Exactive HF-X. The results exhibited that the raw and fresh flavor of raw egg yolks came from 1-Octen-3-ol, 2-Ethyl-1-hexanol, 1-Decene, and 1-Undecene. 2-Methyl-3-octanone, Toluene, and some aromatic compounds worked synergistically to contribute to the aroma profile of boiled eggs. (+)-2-Bornanone, Octanal, 2-Methyl-butanal, Nonanal, (.+Dihydro-3-hydroxy-4,4-dimethyl-2(3 H)-furanone, 6,7-Dihydro-2,5-dimethyl-5 H-cyclopentapyrazine, (E)-2-methyl-6-(1-propenyl)-pyrazine, 2-Ethyl-3,5-dimethyl-pyrazine, 3,5-Diethyl-2-methyl-pyrazine, 2,5-Dimethyl-pyrazine were the key flavor compounds in the fried egg and gave it the popcorn and roasted flavors. The statistical analysis of the lipid profile revealed that brief, high-temperature heating (100 or 200 °C for 10 min) in typical boiled and fried quail eggs had minor effects on the lipid nutritional value. PE-related lipids, particularly those containing 18-carbon fatty acids, contributed to the aroma formation of fried quail eggs.Graphical

Study of Making Gluten-Free Fettuccine from Taro Flour (Colocasia esculenta L. Schoot)

We often encounter fettuccine pasta in cafes and restaurants, usually made from wheat flouras the main ingredient. Wheat flour is a food ingredient that contains gluten, which is a proteincompound from cereals that cause allergies and irritate the small intestine and can also causeceliac (autoimmune) disease in certain people. Several studies have substituted wheat flourfor making fettuccine with non-gluten taro flour. The formula obtained is still a mixture of taroflour and wheat flour. This research used 100% pure taro flour with additional ingredients oftwo types of egg yolk, namely chicken egg yolk and duck egg yolk. This research aimed todetermine the organoleptic results of fettuccine from 100% taro flour based on theformulation using the type of egg yolk and boiling time. This study used a completelyrandomized trial (CRD) design with four treatments and two repetitions. The types oftreatment in this study were the use of duck eggs with a boiling time of 35 seconds, the use ofboiled duck eggs for 1 minute, the use of boiled chicken eggs for 35 seconds, and the use ofboiled chicken eggs for 1 minute. The research results showed that fettuccine was successfullymade using 100% taro flour without being mixed with wheat, using additional egg yolk as abinder. A perfect assessment was obtained from the expert panelists for the duck egg yolkformula boiling for 1 minute. The type of egg yolk and boiling time affect the sample in termsof taste, color, and texture, but not aroma.

Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p&gt;0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.

ОПРЕДЕЛЕНИЕ ИНТЕНСИВНОСТИ ВЕНТИЛЯЦИИ ИНКУБАТОРА ПО ДИНАМИКЕ РАСХОДА ВОДЫ В УВЛАЖНИТЕЛЕ

It is known that the air composition in the incubator chamber is critically important for the development of bird embryos, since the embryo consumes oxygen and produces carbon dioxide during development. High embryonic mortality is observed with insufficient ventilation of the incubator chamber, however, excessive ventilation leads to unreasonable costs of electricity and water in the humidification system. Gas analyzers that allow precise regulation of gas exchange between the incubator chamber and the surrounding air are not used in tabletop incubators, and the problem of determining the optimal ventilation parameters for tabletop incubators remains relevant at present. The possibility of interpreting data on water consumption by humidification systems of tabletop incubators in order to determine the characteristics of oxygen and carbon dioxide gas exchange between the air in the incubator chamber and the environment was studied. Grumbach tabletop incubators, a carbon dioxide detector based on the ZGm053UK IR sensor, and an AT-8100 electrochemical oxygen detector were used. It was established that the dynamics of water consumption of the incubator humidification system correlates with the volume of gas exchange between the incubator and the environment. The danger of stopping the respiratory activity of embryos occurs in sealed incubators, while the marker of the absence of gas exchange is a constant water level in the humidification system. With the rate of water evaporation by the evaporator of at least 3 cm3 / day per 1 chicken egg, the difference in relative humidity in the incubator room and the incubator chamber of at least 10% and the difference in temperatures in the incubator and the incubator room of at least 10 ° C, the gas exchange of the incubator with the room provides a carbon dioxide level of no more than 3000 ppm, and an oxygen level of at least 20.6%. The air composition in the incubator room has a direct effect on the air composition in the incubator, however, the required ventilation level of the incubator room, providing satisfactory air quality, can be determined organoleptically.

A novel QbD HPLC approach for the concurrent analysis of amoxicillin, amprolium, and ethopabate in food samples and dosage forms

A simple, rapid, and sensitive reversed-phase HPLC method coupled with photodiode array detection (PDA) was developed for the simultaneous estimation of three co-administered antibacterial drugs, amprolium, ethopabate, and amoxicillin for the first time. An analytical quality-by-design (QbD) approach was adopted to optimize the developed approach. A two-level full factorial design (25 FFD) was implemented to optimize how variable factors affected chromatographic responses. On a Hypersil BDS C18 column, the chromatographic separation was carried out using isocratic elution at 40.1 °C. The mobile phase consisted of methanol: potassium phosphate buffer containing 5.76 mM heptane sulphonate (9.05 mM; pH 3.32, 44.8:55.2 % v/v) pumped at a flow rate of 0.8 mL/min and an injection volume of 20.0 μL. In less than 7 min, the suggested method was able to separate the ternary mixture with a challenging ratio of 1: 15.6: 1 for amoxicillin, amprolium, and ethopabate, respectively. The calibration curves showed excellent linearity over the concentration ranges of 0.50–30.0, 0.30–30.0, and 0.05–20.0 μg/mL, respectively. Recovery percentages ranged from 90.30 % to 111.13 % with %RSD less than 2 % were obtained upon spiking to chicken tissues, liver, and egg samples, indicating the suitability of the proposed method for determining of the studied drugs in complicated food matrices. Furthermore, the proposed method was successfully applied to determine the studied drugs in their commercial formulations. Full validation was carried out according to ICHQ2 (R1) guidelines. Finally, the method's greenness and ecofriendliness were evaluated using various metrics tools, including the Green Analytical Procedure Index (GAPI) and Analytical GREEnness (AGREE).

The effect of feeding a mixture of shellfish meal and fish oil on egg production and egg storability of Lohmann Brown laying hens

The aim of the research was to determine the effect of feeding a mixture of shellfish (SF) and fish oil (FO) on egg production performance and storability of Lohmann Brown hen eggs. The study used a completely randomized design consisting of 4 treatments and 4 replications, and each replication consisted of 40 Lohman Brown hens with the same laying age (45 weeks of age). The four treatments were mixed levels of shellfish flour with fish oil in feed, namely 0%, 1%, 2%, and 3%, as treatments P0, P1, P2, and P3. Egg samples were taken at the end of the study, then stored and observed for 4 weeks. The results showed that egg pH and haugh units (HU) in the P2 and P3 hen groups significantly (P&lt;0.05) increased. Meanwhile, egg shell weight and egg yolk color did not show significant differences (P&gt;005). Feeding SFFO to P2 and P3 hen groups increased (P&lt;0.05) egg weight, hen-day-production and feed efficiency (feed consumption/egg weight). It can be concluded that SFFO can be used as an ingredient in laying hens with an inclusion level of 20-30g/kg and can be used in chicken feed during the finishing phase. SFFO may affect the storability of Lohmann Brown hen eggs.

Manufacture and Characterization of Polymer Concrete Using Duck Egg Shell Powder, Areca Nut Fiber, and Epoxy Resin

Manufacture and Characterization of Polymer Concrete Using Duck Egg Shell Powder, Areca Nut Fiber, and Epoxy Resin

Osteogenic and angiogenic potential of molybdenum-containing mesoporous bioactive glass nanoparticles: An ionic approach to bone tissue engineering

Biomaterials intended for application in bone tissue engineering (BTE) ideally stimulate osteogenesis and angiogenesis simultaneously, as both mechanisms are of critical importance for successful bone regeneration. Mesoporous bioactive glass nanoparticles (MBGNs) can be tailored towards specific biological needs, for example by addition of ions like Molybdenum (Mo). While Mo has been shown to enhance osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells (BMSCs) as well as their ability to form and mature a primitive osseous extracellular matrix (ECM), there are contradictory findings regarding its impact on angiogenesis. In this study, the effects of Mo-MBGNs (mol%: 70 SiO2, 25 CaO, 5 MoO3) on viability, proliferation, osteogenic differentiation, ECM formation and angiogenic response of BMSCs were compared to undoped MBGNs (in mol%: 70 SiO2, 30 CaO) and a control group of BMSCs. Furthermore, a human umbilical vein endothelial cells tube formation assay and a chorioallantoic membrane-assay using fertilized chicken eggs were used to analyze angiogenic properties. Mo-MBGNs were cytocompatible and promoted the proliferation of BMSCs. Furthermore, Mo-MBGNs showed promising osteogenic properties as they enhanced osteogenic differentiation, ECM formation and maturation as well as the gene expression and protein production of relevant osteogenic factors in BMSCs. However, despite the promising outcome on osteogenic properties, the addition of Mo to MBGNs resulted in anti-angiogenic effects. Due to the high relevance of vascularization in-vivo, the anti-angiogenic properties of Mo-MBGNs might hamper their osteogenic properties and therefore might restrict their performance in BTE applications. These limitations can be overcome by the addition of ions with distinct pro-angiogenic properties to the Mo-MBGNs-composition. Due to their promising osteogenic properties, Mo-MBGNs constitute a suitable basis for further research in the field of ionic (growth factor free) BTE.