Chemical Constituents Of Essential Oil Research Articles

Chemical constituents of essential oils from Thymus vulgaris and Cymbopogon citratus and their insecticidal potential against the tomato borer, Tuta absoluta (Lepidoptera: Gelechiidae)

This study aimed to determine the chemical composition and insecticidal activity of essential oils from Thymus vulgaris (thyme) and Cymbopogon citratus (lemongrass) against the devastating pest, Tuta absoluta. Essential oils were extracted by hydrodistillation and chemically analyzed by Gas chromatography coupled to mass spectrometry. Their knockdown (KD50) and larvicidal (LD50) activities were assessed through direct contact and fumigation toxicity assays. The major components of thyme oil were Thymol (21.53%), α-Pinene (Dextro) (17.43%) and o-Cymene (15.37%), while, Neral (34.48%), Geranial (34.37%) and β-Myrcene (12.84%) were prominent in lemongrass oil. Statistical analysis indicated that both oils exhibited similar knockdown and insecticidal efficiencies through direct contact and fumigation routes. The resultant biological parameters for lemongrass and thyme oils were KD50 values of 0.193 µL/mL and 0.592 µL/mL and LD50 values of 0.328 µL/mL and 0.608 µL/mL respectively for contact toxicity and KD50 of 1.290 µL/mL and 2.565 µL/mL and LD50 of 1.479 µL/mL and 3.046 µL/mL for lemongrass and thyme oils respectively for fumigant toxicity. The reference insecticide, Lynx (λ-cyhalothrin 15 g/L + acetamiprid 20 g/L) elicited no mortality through both routes after 4 hours of exposure. Both essential oils prolonged life cycle of insect pest through both routes as compared to the reference insecticide. However, greenhouse and open field trials are required to validate this approach prior to its implementation for T. absoluta control in tomato farms. To our knowledge, this study reports for the first time the insecticidal potential of lemongrass essential oil against T. absoluta.

Cholesterol-lowering activity of natural mono- and sesquiterpenoid compounds in essential oils: A review and investigation of mechanisms using in silico protein-ligand docking.

Mono- and sesquiterpenoids are the main chemical constituents of essential oils. Essential oils and their constituents have received increasing attention for lipid-lowering properties in both cell and animal models. Despite the chemical diversity of essential oil compounds, the effects of many of these compounds on cholesterol metabolism are highly similar. In this report, we review the literature regarding the effects of essential oils and their terpenoid constituents on cholesterol homeostasis, and explore likely mechanisms using protein-ligand docking. We identified 98 experimental and seven clinical studies on essential oils, isolated compounds, and blends; 100 of these described improvements either in blood cholesterol levels or in sterol metabolic pathways. Our review and docking analysis confirmed two likely mechanisms common to many essential oil compounds: (1) direct agonism of peroxisome-proliferator-activated receptors, and (2) direct interaction with sterol-sensing domains, motifs found in key sterol regulatory proteins including sterol regulatory element binding protein cleavage activating protein and HMG-CoA reductase. Notably, these direct interactions lead to decreased transcription and accelerated degradation of HMG-CoA reductase. Our work suggests that terpene derivatives in essential oils have cholesterol-lowering activity and could potentially work synergistically with statins, however, further high quality studies are needed to establish their clinical efficacy.

Assessing and integrating the transcriptome analysis with plant development, trichomes, and secondary metabolites yield potential in Mentha arvensisL

Mentha arvensisL. (corn mint) is well known for the production of menthol, a used commodity in flavouring industries, and provides natural fragrances. Glandular trichomes are responsible for producing specific secondary metabolites in vascular plants having species chemistry. Ten cultivars/varieties of M. arvensis, namely, Saksham, Kosi, Himalaya, Gomti, Sambhav, Kalka, Damroo, Kushal, and Shivalik, were used to study the developmental regulation of trichomes, essential oil yield, chemical constituents of essential oil and morphological parameters were estimated with gene expression using a randomized block design. Simultaneously, RNA sequence-based transcriptome analysis was done to reveal the transcription factors and differential gene analysis, which are responsible for the biosynthesis of essential oil as well as trichome development. Plant growth showed the maximum transition between 35 and 50 days stage, while essential oil and its metabolite bioconversion was observed in between 70 and 100 days stage. Glandular trichomes were maximally increased between 50, 70 days, and 100 days stage in var. Kosi followed by var. Saryu which has rapid growth in oil content. Menthol reductase activity was found to be a regulatory element during development, as it follows the inverse trend of menthol content and leads to menthol accumulation in subcuticular spaces. Transcriptional factors, cog, and nonredundant novel genes were identified. The composition of mintessential oils is regulated at multiple levels, including transcript abundance, catalytic properties of enzyme catalysts, and cell type-specific epigenetic processes.

Metabolic Profile, Bioactivities, and Variations in the Chemical Constituents of Essential Oils of the Ferula Genus (Apiaceae).

The genus Ferula is the third largest and a well-known genus of the Apiaceae family. It is categorized in the Peucedaneae tribe and Ferulinae subtribe of the Apiaceae family. At present, about 180 Ferula species have been reported. The genus is mainly distributed throughout central and South-West Asia (especially Iran and Afghanistan), the far-East, North India, and the Mediterranean. The genus Ferula is characterized by the presence of oleo-gum-resins (asafoetida, sagapenum, galbanum, and ammoniacum) and their use in natural and conventional pharmaceuticals. The main phytochemicals present in the genus Ferula are as follows: coumarin, coumarin esters, sesquiterpenes, sesquiterpene lactones, monoterpene, monoterpene coumarins, prenylated coumarins, sulfur-containing compounds, phytoestrogen, flavonoids and carbohydrates. This genus is considered to be a valuable group of medicinal plants due to its many different biological and pharmacological uses as volatile oils (essential oils). Numerous biological activities are shown by the chemical components of the essential oils obtained from different Ferula species. Because this genus includes many bioactivities such as antimicrobial, insecticidal, antioxidant, cytotoxic, etc., researchers are now focusing on this genus. Several reviews are already available on this particular genus, including information about the importance and the uses of all the phytochemicals found in the species of Ferula. Despite this, no review that specifically provides information about the biological activities of Ferula-derived essential oils, has been published yet. Therefore, the present review has been conducted to provide important information about the chemical profile, factors affecting the chemical composition, and biological activities of essential oils of the Ferula species.

Bioactivities and Chemical Constituents of Essential Oils Extracted from Caryopteris mongholica Bunge against Two Stored-Product Insects

This work investigated the chemical constituents of the essential oil (EO) extracted from different organs (whole plants, flower and fruits, leave and branches) of Caryopteris mongholica. At the same time, the fumigant, contact and repellent effect of the EO from C. mongholica against Lasioderma serricorne and Tribolium castaneum were evaluated. The main chemical components were identified from the EO included β-pinene (24.77 %), 4-terpineol (15.80 %), β-cis-ocimene (9.31 %), α-pinene (8.20 %), 2-isopropyltoluene (7.37 %). The results of bioassays indicated that EO was toxicity to T. castaneum than L. serricorne. The results also showed that possessed stronger repellent activity against T. castaneum than L. serricorne. The results provided a basis for the development and utilization in the future for the control of insects.

Variation in chemical constituents of essential oils of the fresh, dried and fermented leaves of Premna serratifolia

Variation in chemical constituents of essential oils of the fresh, dried and fermented leaves of Premna serratifolia

Chemical Composition and Antimicrobial Activities of the Essential Oil From the Leaves of Pterocephalus hookeri

Detailed chemical constituents of essential oil from the Pterocephalus hookeri leaves and its antimicrobial activities were investigated in this study. The essential oil, obtained by hydrodistillation, was characterized by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analyses. Among the 90 identified compounds, hexadecanoic acid (21.27%), phytol (8.03%), furfural (7.08%), oleic acid (5.25%), and phytone (4.56%) were the major components. In the antimicrobial assay, the essential oil showed strong inhibitory activities against Escherichia coli, Candida albicans, and Staphylococcus aureus with minimum inhibitory concentration values of 31.3, 62.5, and 125 µg/mL, respectively. To our knowledge, this is the first report concerning chemical composition and antimicrobial activities of the essential oil from Pterocephalus hookeri.

Screening of radical scavenging activity and chemical constituents of the essential oil from star anise by ultra‐fast GC electronic nose coupled with DPPH, OH, and ABTS assays

A sensitive and selective method of ultra-fast GC electronic nose (E-nose) coupled with chemical methodology was developed for both identifying the chemical constituents of essential oil from star anise (EOSA) and the screening of the radical scavenging activity of each compound in EOSA quickly without the isolation of monomers by comparing the change of the chromatographic peak area of every component in EOSA before and after the reaction with the free radicals. The results showed the main components of EOSA were anethole (36.42%), limonene (20.77%), α-terpinene (6.51%), and α-phellandrene(5.61%). Among all chemical components, p-menthatriene showed the strongest scavenging ability both in 1,1'-diphenyl-2-picrylhydrazine (DPPH) and hydroxyl (OH) radicals, and p-cymenene showed the strongest scavenging ability in 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulphonate) (ABTS) radical, with scavenging rates of 93.00 ± 0.56%, 79.50 ± 0.78%, and 70.80 ± 0.32%, respectively. The reliability and feasibility of using E-nose to identify chemical constituents of EOSA were verified by gas chromatography-tandem mass spectrometry(GC-MS/MS). Practical applications The essential oils are the complex mixtures and important constituents in natural edible spices. How to evaluate and screen the radical scavenging activity of each component in the mixtures is a very challenging project. The traditional chemical methods are mainly used to evaluate total radical scavenging activity and antioxidant activity of essential oils. The compounds in the mixture need to be separated into individuals in advance if we want to know the bioactivity of each component and their radical scavenging activity in the past. The paper has developed a simple and convenient ultra-fast GC E-nose method. This method can not only quickly identify the chemical components of essential oil from star anise, but also quickly evaluate the radical scavenging activity of each compound in the essential oil from star anise. The proposed method can be applied to almost any other essential oils and volatile mixtures.

Preparation and Chemical Analysis of Volatile Oil in Two Different leaf type of Ficus hirta Vahl.

To analyze the chemical constituents of volatile oil in two different leaf types of Ficus hirta Vahl. Steam distillation was used to collect the different leaf types of Ficus hirta Vahl., the volatile chemical components of extracts were analyzed using GC-MS,and the relative contents of various chemical components in the volatile oils of the two medicinal materials were calculated by area normalization method. The volatile oils of the Ficus hirta Vahl of three-lobed leaf type and five-lobed leaf type contained 38 and 39 compounds, respectively. The main components are coumarins, organic acids, aldehydes, esters, alcohols, etc. In addition, there are some Hydrocarbons, phenols, ketones, and the chemical composition and relative content of volatile oils of the two have large differences.The method can quickly and easily identify the chemical constituents of the volatile oils of two different leaf Ficus hirta Vahl., and there are significant differences in the chemical constituents in the volatile oils of the Ficus hirta Vahl. of three-lobed leaf type and five-lobed leaf type.

Correlation and path-analysis for morpho-economic traits and chemical constituents of essential oil in Corn mint (Mentha arvensis L.) accessions.

Mentha arvensis L . which belongs to Lamiaceae family and it is also know as cornmint or japanese mint.The plant as been used for medicinal and aromatic purpose since from ancient time. The present study was planned to estimate the variability among morpho-economic traits, to estimate magnitude of association between essential oil yield and its contributing traits and to determine the direct and indirect effects of various agro-morphic traits on essential oil yield. Experimental materials consist of twenty-nine accessions, which were evaluated for two consecutive years (2016-2017 and 2017-2018) in a randomized complete-block design (RCBD) with two replications. Data were collected on agro-morphic traits and essential oil yield. The results revealed that, the plant height was ranged from 72 cm (MASP-20) to 31cm (MASP-2), herb yield per plot was ranged from 1.83 kg/plot (MASP-13) to 4.36 kg/plot (MASP-12), and essential oil yield was varied from 0.118 kg/plot (MASP-1) to 0.698 kg/plot (MASP-12).The menthol percent was varied from 0.13% (MASP-15) to 73.19 % (MASP-24),menthyl acetate 25.52% (MASP-29) to 0.030% (MASP-18), pulegone 0 % to 75.00% (MASP-22), and limonene was varied from 0.24 %(MASP-29) to 24.89% (MASP-27).The significant and positive association was noticed between essential oil yield and herb yield per plot (0.82**; ** = significant at 1% probability level) and number of branches per plant (0.26*; *= significant at 5% probability level). Among the chemical constituents of essential oil, Limonene percent was positively and significantly correlated with pulegone percent. The path coefficient analysis revealed that herb yield per plot had the highest direct effect on essential oil yield (0.8104). Plant height, number of branches per plant, and herb yield per plot can be used as a selection criterion during selection to develop a high yielding menthol mint chemotypes.

GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS IN ESSENTIAL OILS OF LEAF OF EUODIA SUAVEOLENS SCHEFF

Objective: Essential oils extracted from the leaf of Euodia suaveolens have been reported. However, there is no published data on comprehensive report on the chemical constituents of the essential oils and the methods utilized to extract the essential oils from the plant. This research aimed to reveal and determine the chemical constituents of essential oils from the leaf of E. suaveolens.
 Methods: Essential oils of leaf of E. suaveolens were extracted by steam distillation and were analyzed utilizing Gas Chromatography-Mass Spectrometry (GC-MS) methods.
 Results: The GC-MS analysis revealed the presence of twenty-five different chemical constituents from the essential oils. The main chemical constituents of essential oils extracted from E. suaveolens’ leaves were as follows menthofuran (50.38 %), p-mentha-1,8-diene (14.34 %), limonen (10.99 %), evodone (5.55 %), α-curcumene (4.65 %), globulol (1.88 %), longipinenepoxide (1.66 %), and linalool (1.40 %). This present research found three compounds, namely p-mentha-1,8-diene, globulol, and longipinenepoxide that have never been reported by any researchers working with this plant.
 Conclusion: The results showed that E. suaveolens contains essential oils that are potential to be explored further and utilized as medicinal products against some ailments.

Comparison of Chemical Constituents of Essential Oil from Aerial Constituent of Mentha longifolia and Salvia multicaulis Obtained by Hydro distillation and Headspace Analysis

As an important phytochemical class, essential oils of aromatic plants are determined as a perfect resource of food additives and medicinal agents. The chemical composition of the essential oils is affected by many factors such as extraction techniques. The present study focused on a comparative study of the chemical constituent of essential oil acquired by hydrodistillation and that by headspace analysis. Salvia multicaulis: There was a major constituent in the essential oils which were obtained through the extraction by the HD approach. These compounds included α-pinene (15.4 %), 1,8-cineole (10.2 %), camphene (8.1 %), β- pinene (3.9 %), camphor (6.6 %), borneol (8.8 %), bornyl acetate (10.3% ) and (E) -caryophyllene (9.9 %). The HS managed to yield α-pinene (41.2 %), camphene (19.3% ), 1,8-cineole (11.1% ), β-pinene (5.0 %), myrcene (2.3 %), limonene (4.1 %) and camphor (3.6 %). Mentha longifolia: Outcomes of GC/MS analysis have identified 28 and 29 constituents in samples removed by HS and HD approaches; representing 99.7 % and 99.5 % of the oils. pulegone (64.0 ), 1,8-cineole (11.7 %), piperitenone (4.5 %), p-menth-3-en-8-ol (2.7% ), borneol (2.7 %), β-pinene (2.1 %), cis-isopulegone (2.0 %), α-pinene (1.5% ) and sabinene (1.5 %) were considered as huge parts of essential oil removed by HD and significant constituent of headspace constituent by HS procedures were pulegone (41.4 %), β-pinene (6.1 %), 1,8-cineole (21.7 %), α-pinene (5.7 %), p-menth-3-en-8-ol (5.0 %), sabinene (4.4 %), myrcene (3.8 %), limonene (2.2 %). In conclusion, it has been suggested that the headspace approach be used for essential oil extraction since it is simple, time-saving, and eco-friendly.

Efficacy of bioactive compounds and their association with different cowpea cultivars against their major stored pest.

Stored grain insects are controlled with fumigant insecticides which can select resistant insect populations and cause environmental and applicator contamination. Thus, resistant cultivars and chemical constituents of essential oils are an alternative to the almost exclusive use of these insecticides. The effects of the combination of cowpea cultivars Vigna unguiculata (L.) Walp. with chemical constituents of essential oils against Callosobruchus maculatus were determined. Four cowpea cultivars: BRS Tracuateua, BR 17 Gurgueia, Epace 10 and Sempre Verde (insect rearing) untreated were used in the experiments and combined with chemical constituents of essential oil: eugenol, geraniol and trans-anethole. The biological parameters observed were: total egg number and eggs per grain, egg viability (%), insects emerged and insects per grain, immature stage viability (%), instantaneous rate of growth (ri), insect dry weight (mg), grain weight loss (%) and egg-adult period. When comparing all biological parameters, the cultivars BRS Tracuateua and BR 17 Gurgueia were harmful to C. maculatus. In the toxicity tests, the results showed that LC30 and LC50 of the chemical constituents ranged from 54.77 to 103.48 ppm and 60.99 to 125.18 ppm, respectively. In most of the biological parameters, LC50 had adverse effects significantly higher than LC30 and BR 17 Gurgueia treated were harmful to C. maculatus. Overall, the findings showed that BR 17 Gurgueia combined with eugenol and geraniol more significantly affected the biological parameters of C. maculatus than when associated with trans-anethole, reducing egg number, insects emerged and egg viability. © 2020 Society of Chemical Industry.

GC-MS analysis of chemical constituents of volatile oil from stems and leaves of Schefflera arboricola Hagata

GC-MS analysis of chemical constituents of volatile oil from stems and leaves of Schefflera arboricola Hagata

The Synergistic Effect of Arbuscular Mycorrhizal Fungi and Pseudomonas fluorescens on Growth and Qualitative and Quantitative Yield of Thymus kotschyanus Essential Oil

In this study, to investigate the synergistic effect of Pseudomonas fluorescens and arbuscular mycorrhizal fungi on the qualitative and quantitative yield of Thymus kotschyanus essential oil, an experiment was conducted in a randomized complete block design with five treatments and three replications at the Alborz Research Complex, Research Institute of Forests and Rangelands. The treatments used included Pseudomonas fluorescens, P. fluorescens and Rhizophagus clarus, P. fluorescens and Funneliformis badium, P. fluorescens and Acaulospora laevis, control or without inoculation. After growth and harvesting stages, the essential oil was extracted and then analyzed by gas chromatography to identify the compounds. According to the results, the highest shoot height of Thyme was related to the P. fluorescens and A. laevis treatment, the highest vegetation cover belonged to the R. clarus and P. fluorescens treatment, and the highest number of branches and total biomass was recorded in the P. fluorescens and A. laevis treatment. The highest essential oil percentage belonged to the P. fluorescens treatment. The main phytochemicals of the essential oil were p-cymene, 1,8- cineol, δ-terpinene, borneol, geraniol, geranial, thymol, carvacrol, geranyl acetate, E-caryophyllene, and the highest content of geraniol, geranial, and geranyl acetate was detected in the P. fluorescens treatment. The highest content of thymol was in the A. laevis and P. fluorescens treatment, and the highest content of carvacrol was in the R. calalus and P. fluorescens treatment. The results of this study show that the inoculation of Pseudomonas fluorescens is a promising inoculum for the optimum production of medicinal plants. These microorganisms can also be used to enhance the performance and production of chemical constituents of essential oils and organic production.

Analysis of Essential Oils from Leaf of Syzygium hancei Merr. & Perry, Syzygium caryophyllatum (L.) Alston and Syzygium lineatum (DC.) Merr. & Perry from Vietnam

The essential oils obtained from the leaf of Syzygium hancei Merr. & Perry, Syzygium caryophyllatum (L.) Alston and Syzygium lineatum (DC.) Merr. & Perry were analysed using gas chromatography flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). The yields of the essential oils were 0.15, 0.18, and 0.15 (v/w) respectively, calculated on a dry weight basis. The main constituents of the oils were γ-guaiene (11.07 %), β-caryophyllene (9.11 %), cis-calamenene (7.46 %), α- copaene (6.97 %), trans-cadina-1,4-diene (5.09 %) were identified in the leaf of S. hancei. β-caryophyllene (42.53 %), (E)-β-ocimene (19.38 %) and α-humulene (5.37 %) were identified in the leaf of S. caryophyllatum. β- caryophyllene (64.53 %), α-pinene (6.14 %), α-selinene (4.76 %) and α-humulene (4.39 %) were identified in the leaf of S. lineatum. The chemical constituents of essential oils from the leaf of S. hancei and S. lineatum are being reported for the first time. The β-caryophyllene content found S. caryophyllatum in Vietnam was higher than in other region. S. lineatum with a large percentage of β-caryophyllene could be a source to exploit this compound.

Chemical composition of essential oil from the leaves of Amentotaxus yunnanensis H.L. Li in Ha Giang province

The essential oil from the leaves of the Amentotaxus yunnanensis collected in Hoang Su Phi, Ha Giang province was obtained by hydrodistillation using a Clevenger apparatus and the yield of the essential oil was 0.01% from air-dry material. By using GC/MS analysis 50 constituents were identified, accounting for 88.96% of the essential oil. The main constituents were α-pinene (21.91%), kaur-16-ene (13.03%), α-calacorene (9.42%), δ-cadinene (6.23%) and β-caryophyllene (4.9%). This is the first study on the chemical constituents of essential oils from the leaves of Amentotaxus yunnanensis in Vietnam.
 Keywords: essential oil, Amentotaxus yunnanensis, α-pinene, kaur-16-ene, α-calacorene, δ-cadinene.

Antimicrobial Activity and Chemical Constituents of Essential Oil from the Leaves of Alpinia globosa and Alpinia tonkinensis

This paper reports the chemical constituents and the antimicrobial activity of essential oils hydrodisitlled from the leaves of Alpinia globosa Hour (syn. Amomum globosum Lour.) and Alpinia tonkinensis Gagnep. The essential oils were analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS) techniques. The minimum inhibitory concentration (MIC) values were measured by the microdilution broth susceptibility assay. The absolute yields of the essential oils were 0.16 % (v/w, ± 0.01) and 0.21 % (v/w, ± 0.02), respectively for A. globosa and A. tonkinensis, calculated on a dry weight basis. The major constituents of A. biglobosa were β-pinene (12.1 %), α-gurjunene (10.5 %) and (Z)-13-docosenamide (9.0 %). The compounds occurring in higher amount in A. tonkinensis includes β-pinene (33.5 %), (E)-β-ocimene (9.6 %), γ-terpinene (9.2 %) and α-pinene (8.4 %). The essential oil of A. tonkinensis inhibited the growth of Saccharomyces cerevisiae (ATCC 16404) with MIC value of 25.0 μg/mL. In addition, both essential oils displayed antimicrobial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus subsp. aureus (ATCC 11632) and Fusarium oxysporum (ATCC 48112) with MIC value of 50.0 μg/mL. The chemical constituents and antimicrobial activity of the studied essential oils were being reported for the first time.

Chemical Composition and Antitermite Activity of Essential Oil from Artemisia absinthium Growing in Kashmir Valley of India

Artemisia absinthium is a herbaceous perennial plant with fibrous root and it belongs to the family Asteraceae. Due to having strong fragrance and pungent taste it has ability to defend itself from the herbivore animals. Essential oil extracted from leaves, stem and seeds of A. absinthium were analyzed for termite mortality as well as termite repellency. Chemical constituents of essential oil from all three parts were identified and compared by gas chromatography-mass spectroscopy. Total 16 compounds identified in the essential oil of leaves, stem and seeds in which boronyl acetate was present in major amount 26.59 %, 27.17 % and 26.63 % respectively. L-terpenen-4-ol was also identified in higher amount in leaves, stem and seeds; 18.24 %, 17.07 % and 17.3 % respectively. Leaf, stem and seed essential oil tested for termite mortality and repellency against Microcerotermes beesoni. Leaf essential oil showed significant and seed essential oil all were found to have moderate activity.

Antimicrobial Activity and Chemical Constituents of Essential Oils from the Leaf and Wood of Taxus chinensis (Rehder & E.H. Wilson) Rehder (Taxaceae) from Vietnam

This paper report the volatile compounds identified in the leaf and wood essential oil hyrdrodistilled from Taxus chinensis (Rehder & E.H. Wilson) Rehder (Taxaceae) from Vietnam. The chemical constituents of the oil were analysed by gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS). The yields of the yellow oils were 0.15 % and 0.11 % (v/w) respectively, calculated on a dry weight basis. The main constituents of the leaf oil were monoterpenes represented by α-pinene (24.2 %), sabinene (19.5 %), α-terpinyl acetate (12.8 %) and 1,8-cineole (11.7 %). However, α-pinene (20.0 %), photosantalol (10.2 %), caryophyllene oxide (8.9 %), spathulenol (7.6 %) and guaiol (6.8 %) were the representative compounds of the wood oil. The essential oils displayed antimicrobial activity against Enterococcus faecalis ATCC 299212 with MIC values of 16.0 and 64.0 μg/mL, and IC50 of 3.98 and 20.33 μg/mL, respectively. However, only the leaf oil inhibited the growth of Bacillus cereus ATCC 14579 with MIC and IC50 values of 64.0 and 19.78 μg/mL, while showing stronger action towards Candidan albicans ATCC 10231 with MIC of 128.0 μg/mL. Moreover, the wood oil exhibited stronger antimicrobial activity against Staphylococcus aureus ATCC 25923 (MIC 128.0 μg/mL, IC50 56.78 μg/mL) and displayed moderate antimicrobial action against Escherichia coli ATCC25922 with MIC and IC50 values of 256.0 and 87.78 μg/mL respectively.