Treatment Of Cheese Whey Research Articles

Sustainable biogas production via anaerobic co-digestion of cheese whey and cattle manure

In Mexico, specifically in Chiapas, the dairy cattle industry plays a significant role in cheese and milk production. However, the large quantities of cattle manure (CM) and cheese whey (CW) generated as byproducts pose environmental challenges if not managed properly. To address this issue, anaerobic digestion (AD) technology offers a sustainable solution by treating organic waste and producing biogas. This research study focuses on assessing the potential of CW and CM, both individually and in co-digestion, in an anaerobic environment. The study also evaluates biogas yield and composition using an up-flow anaerobic sludge blanket (UASB) reactor with different CW and CM mixtures. The findings indicate that the 30CM:70CW ratio exhibited the highest methane yield, surpassing other assays in co-digestion and mono-digestion. Furthermore, the UASB reactor showed that a mixture of 90CW:10CM produced 25.73 L of biogas per gram of volatile solids daily, comprising 60 % methane and 40 % carbon dioxide. This research demonstrates the potential for efficient and environmentally friendly treatment of CM and CW through optimized co-digestion and UASB technology, highlighting the opportunity to generate biogas while reducing waste.

Cheese whey generation, management and potential for biogas production in Mexico and the State of Jalisco

ABSTRACT Pollution of water bodies and process upset in treatment plants in Mexico by cheese whey discharges is a matter of concern where scant inspection and law enforcement may aggravate the situation. This study analyzes the current state of environmental regulatory compliance and the impacts of whey discharges with particular emphasis on the country’s leading dairy-producing state, Jalisco. Subsequently, cheese whey characteristics and current valorisation volume and uses are discussed in detail. From there, anaerobic digestion is presented as a key technology for cheese whey treatment mainly to comply with wastewater regulations, with recovery of biogas as bonus. From there, the volume of cheese whey discharged to the environment and potential equivalence in biogas and electricity production is calculated considering both the technologies commonly used in industrial applications and for farms and small producers. Also, the potential electricity generation and prevented atmospheric impact are calculated and framed against the current Mexican energy and emissions inventory. Finally, a review of past efforts to disseminate the use of anaerobic digesters to produce biogas from organic waste in Mexico is undertaken, followed by a discussion of the basic requirements for a strategy to sustainably manage and treat whey discharges.

Cultivation of a naturally resilient Chlorella sp.: A bioenergetic strategy for valorization of cheese whey for high nutritional biomass production

This study investigated Chlorella's capacity to treat cheese whey (CW) effluent and produce a high-nutritional value biomass, by using a systematic sequential experimental design. Physicochemical analysis of CW revealed its high pollution load, characterized by elevated levels of lactose, phosphorus, and nitrogen, as well as high turbidity due to the presence of whey solids. Screening experiments demonstrated that trace mineral addition and continuous air supply are essential factors for Chlorella biomass production in CW (>800 mg·mL−1). Furthermore, whey solids did not hinder Chlorella growth, with notable biomass production observed even in undiluted CW, demonstrating this microalga's ability to adapt metabolically to the complex environment. Laboratory-scale photobioreactor experiments confirmed Chlorella's ability to produce biomass in CW, outperforming controls (>800 mg·mL−1). Bioremediation potential assessment exhibited significant reductions in organic pollutants (>14 g·L−1 COD), nitrogen (>400 mg·L−1), phosphorus (>140 mg·L−1) and sodium (>650 mg·L−1). CW solids were also removed with Chlorella harvesting (>99 %). Harvested algal biomass was enriched with proteins (>40 g·100 g−1), polyunsaturated fatty acids (>9 % TFA) and pigments, offering potential applications in nutraceutical and pharmaceutical industries. Overall, this study highlights Chlorella's efficacy in CW treatment and biomass valorization, offering a sustainable solution for dairy wastewater management while producing valuable resources.

Tertiary treatment of dairy wastewater applying a microalga-fungus consortium

ABSTRACT This paper aimed to apply filamentous fungi (Penicillium oxalicum and Cunninghamella echinulata), the microalga Tetradesmus obliquus and their co-culture in advanced treatment (tertiary treatment) of cheese whey. The bioremediation process was carried out in agitated flasks and bubble column bioreactors with different concentrations of chemical oxygen demand (COD) (223–1663 mg L − 1), total nitrogen (TN) (13–61 mg L − 1), and total phosphorus (TP) (3–26 mg L − 1). The results obtained in shaken flasks showed a superiority of the consortium compared to the systems with separated species. In this sense, the treatment was carried out in a bubble column reactor, and the consortium formed by the microalga and the fungus C. echinulata showed a greater efficiency (at a light intensity of 100 µmol m − 2 s − 1), promoting by the symbiosis to reach removal efficiencies of up to 93.7, 78.8 and 93.4% for COD, TN and TP, respectively; meeting Brazilian and European standards for discharge into water bodies. In addition, no pH adjustment was required during the co-culture treatment, demonstrating the buffering effect of using these two types of microorganisms. Therefore, the use of the consortium formed by T. obliquus and C. echinulata as a remediator was highly promising to promote the advanced treatment of cheese whey.

Parchmentization process for low cost novel separator for cheese whey treatment in microbial fuel cell

Parchmentization process for low cost novel separator for cheese whey treatment in microbial fuel cell

Revaluation process of cheese whey through the cultivation of black soldier fly larvae (Hermetia illucens)

AbstractOrganic wastes, including cheese whey, are generated in greater quantities each year, which require management that has economic and social implications. Current treatments of cheese whey do not allow for its complete degradation or utilization. In this context, black soldier fly larvae (BSFL) promise to degrade a variety of wastes while generating and accumulating value‐added products such as protein, fat that can be transformed into biodiesel, and fertilizer can be obtained from their residues. In this work, the aim was to evaluate the cultivation of BSFL as a biological treatment for the revaluation process of cheese whey, as well as the composition of the larvae and residue (FRASS) obtained for the subsequent extraction of value‐added products such as fat or protein. It was observed that cheese whey did not affect the growth of the larvae, on the contrary, it increased it (final weight of = 0.252 ± 0.003 g). The larvae biomass was composed of mainly of lipids (42.48 ± 1.26% dry matter) and protein (34.98 ± 0.72% dry matter). A fatty acid profile was also performed, which confirms that, due to their composition (Saturated fatty acids = 262.91 ± 34.81 mg/g; monounsaturated fatty acids = 85.29 ± 19.64 mg/g; polyunsaturated fatty acids = 63.68 ± 11.33 mg/g), these accumulated lipids can be extracted for biodiesel production. This is the first approach to the treatment and utilization of cheese whey through insects.

Integrated production of hydrogen and methane in a dairy biorefinery using anaerobic digestion: Scale-up, economic and risk analyses

Anaerobic digestion has emerged as the most appealing waste management strategy in biorefineries. Particularly, recent studies have highlighted the energy advantages of waste co-digestion in industrial biorefineries and the use of two-stage systems. However, there are some concerns about moving the system from laboratory testing to industrial scale. One of them is the high level of investment that is required. Therefore, this study carried out a techno-economic analysis (scale-up and energy production, economic and risk analysis, and factorial design) to assess the feasibility of single- and two-stage systems in the treatment of cheese whey and glycerin for the production of hydrogen and methane. Scenarios (S1 to S9) considered thermophilic and mesophilic single and two-stage systems with different applied organic loading rates (OLRA). The analyses of scale-up and energy production revealed that S3 (a thermophilic single-stage system operated at high OLRA 17.3 kg-COD.m−3.d−1) and S9 (a thermophilic-mesophilic two-stage system operated at high OLRA 134.8 kg-COD.m−3.d−1 and 20.5 kg-COD.m−3.d−1, respectively) were more compact and required lower initial investment compared to other scenarios. The risk analysis performed by a Monte Carlo simulation showed low investment risks (10 and 11%) for S3 and S9, respectively, being the electricity sales price, the key determining factor to define whether the project in the baseline scenario will result in profit or loss. Lastly, the factorial design revealed that while the net present value (NPV) is positively impacted by rising inflation and electricity sales price, it is negatively impacted by rising capitalization rate. Such assessments assist in making decisions regarding which system can be fully implemented, the best market circumstances for the investment, and how market changes may favorably or unfavorably affect the NPV and the internal rate of return (IRR).

Evaluation of Arthrospira (Spirulina) platensis growth on cheese whey in the context of circular economy

Cultivation of microalgae using wastewater could be considered as a cost-effective treatment method, while providing the necessary nutrients to for the production of high-value biomass. The potential use of cheese whey for Arthrospira (spirulina) platensis cultivation could provide a sustainable solution aligned with circular economy principles for the treatment of dairy industry wastewater, in regions such as North Aegean (Greece). The aim of this research is to investigate the parameters that influence the growth of Arthrospira platensis biomass on whey rich solutions, as a substitute to chemical nutrient feeds, according to the practices of circular economy and sustainable development. Specifically, the effect of different whey dilution ratios and irradiation conditions on biomass productivity, nutrient consumption and COD removal were examined in batch experiments. Results show that the concentration of 10% (v/v) cheese whey exhibited the best performance in both experimental illumination sets; continuous and light/dark cycles. The highest biomass production (1.06 g l−1) was achieved in 14-day experiments at a pH 10.5 and with a photoperiod of 12 h dark/light. In the culture with light/dark cycle, approximately 85% COD removal and 70% NO3−−N consumption were measured. Overall, Arthrospira platensis cultures demonstrate a consistent growth performance in a wide range of conditions and can be considered as an efficient method of treating cheese whey wastewater. Concurrently, Arthrospira biomass production presents interest, due to the low cost associated with the unsterile nature of the cheese whey waste and the utilization of the whey waste as a nutrient for growth.

Application of nanotechnology in anaerobic digestion for biohydrogen production improvement from natural coagulation/flocculation sludge using metallic oxide nanoparticles

ABSTRACT The production of second-generation biohydrogen from organic waste by the dark fermentation process is a solution to reduce the immense volume of waste generated by the food industry. In this study, a coagulation sludge was recovered from the settling tank after treating cheese whey wastewater by natural coagulation/flocculation process. The sludge was used as a substrate for dark fermentation. In the biodigestion study, mixed culture from a biological treatment plant was used as inoculum. The whole process was set under defined conditions to favor hydrogen production. A series of Inoculum/Substrate (I/S) ratios (1/2, 1/3, 1/4, 1/5 and 1/6, g VS inoculum/g VS substrate) were studied in order to determine the optimum ratio for better biohydrogen production. The optimum (I/S) ratio was found to be 1/2 resulting in the highest biohydrogen volume of 237 mL/gVS. The production of biohydrogen as a function of the concentration of different nanoparticles has been studied. Several nanoparticles have been synthetized and tested: CuO, Fe2O3, TiO2. The production of biohydrogen has varied widely depending on the concentration and type of nanoparticles used. All the studied nanoparticles showed an evolution of biogas production. The maximum biohydrogen volume increase was obtained by TiO2 NPs at 0.5 mg/L of concentration noting more than 337% of a biohydrogen volume improvement (total produced volume 800 mL/gVS).

Power Generation from Cheese Whey Treatment by Anaerobic Digestion and Microbial Fuel Cell

Power Generation from Cheese Whey Treatment by Anaerobic Digestion and Microbial Fuel Cell

Integrating process modelling and sustainability assessment to improve the environmental and economic sustainability in the cheese industry

This paper combines process design and modelling with life cycle sustainability assessment to identify opportunities for improving the environmental and economic performance in the cheese industry. Considering both the production and consumption perspectives, the study considers first a range of improvement options in the cheese manufacturing process, followed by an assessment of the rest of the life cycle. For the manufacturing process, the focus is on energy efficiency and valorisation of waste with the following four options considered: Option 1 is a base case that reflects current manufacturing practice and therefore excludes energy recovery and waste utilisation; Option 2 includes heat integration applied to recover waste heat from the process; Option 3 considers the combination of heat integration with treatment of cheese whey via anaerobic digestion to produce biogas; in Option 4, heat integration is combined with treatment of whey via fermentation to produce bioethanol and animal feed. Cheddar cheese has been selected by way of example as one of the most consumed types of cheese in different countries, including the US and the UK. Using life cycle assessment and life cycle costing, the environmental and economic sustainability of cheese have been assessed for these options from farm gate to cheese-plant gate for the production perspective, and from cradle to grave for the consumption perspective. Option 4 is found to be the best alternative across all impacts and costs for both perspectives. For example, compared to the base case, the climate change impact and life cycle costs of cheese are reduced respectively by 148% and 158% for the production perspective, and by 3.4% and 8% for the consumption perspective. These reductions are mainly due to the co-production of bioethanol and animal feed. Production of biogas from whey in Option 3 has higher environmental impacts than the base case, making it the worst alternative. Taking the consumption of cheese at the UK level as an example, improvements in the manufacturing process via Option 4 could reduce the annual climate change impact of the food sector by around 209 kt CO 2 eq./yr and primary energy demand by 6.7 PJ/yr, while increasing the value added of cheese by up to 11%. These results will be of interest to cheese producers, food manufacturers, consumers and policy makers.

Indirect Monitoring of Anaerobic Digestion for Cheese Whey Treatment

Efficient monitoring is an open problem in the operation of anaerobic digestion processes, due to the lack of accurate, low-cost, and proper sensors for the on-line monitoring of key process variables. This paper presents two approaches for the indirect monitoring of the anaerobic digestion of cheese whey wastewater. First, the observability property is addressed using conventional and nonconventional techniques, including an observability index. Then, two model-based observer techniques, an extended Luenberger observer, a sliding mode observer, and a data-driven technique based on fractal analysis are formulated and discussed. The performance and capabilities of the proposed methodologies are illustrated on a validated model with experimental data of the anaerobic digestion of cheese whey. Experimental pH measurements are used for the data-driven approach based on fractal analysis. The experimental data sets correspond to experimental conditions (pH > 7.5 and temperature (T) = 40 °C) favoring volatile fatty acid (VFA) production (30 g/L) with simultaneously acceptable biogas production (3420 mL). Results also show that the proposed observers were able to predict satisfactory key process variables. On the other hand, the fractal analysis provides reliable qualitative trends of VFA production and chemical oxygen demand (COD) consumption.

Role of waste-based geopolymer spheres addition for pH control and efficiency enhancement of anaerobic digestion process.

In anaerobic digestion processes, pH has a vital role due to the direct impacts on the microbial community. An eco-friendly approach has been applied to control pH in anaerobic bioreactors, using waste-containing fly ash geopolymer spheres (GS) instead of powdered chemical compounds, to promote continuous alkalis leaching. The influence of GS porosity and concentration on the behavior of anaerobic sequential batch reactor treating cheese whey was evaluated. Results showed that the use of GS with the highest concentration and porosity promoted an increase in methane yield up to 30%, compared to the assay with powdered chemical compounds addition. In addition, GS boosted butyric acid production to the detriment of propionic acid, which favored methane production by a factor up to 1.2. This innovative approach indicates that GS addition can regulate pH in anaerobic digesters treating challenging wastewaters and, simultaneously, improve not only its efficiency but also the sustainability of the entire process.

Cheese whey co-digestion treatment in a tubular system: microbiological behaviour along the axial axis

This study carried out an anaerobic co-digestion treatment of cheese whey using cattle manure as a co-substrate. A co-digestion process was implemented in three tubular reactors, each one with a different organic load rate (0.5, 1.0 and 1.5 kg CODfed m−3digester day−1). A biochemical and microbiological analysis along the axial axis of each reactor showed that it was possible to obtain a phase separation with an organic load rate of 1.0 kg CODfed m−3digester day−1. The volatile fatty acid production phase takes place in the first reactor section and the consumption phase in the second section. Results from high-throughput sequencing analysis showed differences in the microbial communities between the samples of the three reactors for both eubacterial and archaeal compositions. In the reactor with intermediate organic loading rate, acetoclastic communities predominate, while at lower load, both acetoclastic and hydrogenotrophic communities are detected. Additionally, a microbial consortium stratification was observed.

Red mud-based inorganic polymer spheres: Innovative and environmentally friendly anaerobic digestion enhancers

Red mud-based inorganic polymer spheres were used as alternative pH regulators and process enhancers in sequencing batch anaerobic reactors treating cheese whey. This byproduct tends to quickly acidify under anaerobic conditions, and the common route to control pH and ensure suitable conditions for methane production involves the use of commercial alkaline raw materials. The spheres were synthesized using significant amounts of hazardous and toxic waste, red mud (50 wt% of solid components), whose recycling is challenging. The inorganic polymeric spheres, when compared to virgin alkaline raw materials, improved organic matter removal by 44%, prevented VFA accumulation (acidification degree less than 20%), maintained pH values in a range (6.5–7.2) to ensure maximum methanogenic activity by archaea microorganisms, and boosted the methane volume by ~90%. These promising results demonstrate the feasibility and performance advantages of using these innovative spheres instead of virgin raw materials, which is an important tool towards sustainable development.

Using Matlab's wavelet toolbox to compare electric signals outputted by microbial fuel cells

Motivation: microbial fuel cells (MFC) represents a wastewater treatment technology with the potential for a relevant electric energy generation. The monitoring of the electric current outputted generates time series of data. It was generated two time series using an experimental setup with a low-cost dual chamber microbial fuel cell treating cassava wastewater and, also, it was used the electric current data from a previously described experiment with this same setup treating cheese whey. All of this, innovatively, according our best knowledge, was studied using power spectral density, multifractal and wavelet coherence analysis. Results are promising and indeed point out this approach as an under-considered tool towards gaining knowledge about biofilms attached on anodes of a type of microbial fuel cell reactor.

Second Cheese Whey Treatment Using Zeolite under Continuous Flow Mode and Its Application on Wheat Growth

The efficiency of natural zeolite to treat second cheese whey (SCW) and remove ammonium from artificial wastewater (AWW) was examined. Since zeolite has been reported to improve nitrogen availability in soils, its effect on wheat plant growth was also examined. Continuing a previous study using batch reactors, results are presented concerning experiments in fixed-bed columns under continuous operation. Results from the continuous flow column experiments using AWW and zeolite (2.0–2.8 mm) indicated that low flow rates (4 mL/min and 8 mL/min) did not significantly affect zeolite adsorption ability, while maximum zeolite adsorption capacity reached 15.30 mg NH4+-N/g. Finally, the effect of zeolite saturated with NH4+-N on plant growth was examined. The application of saturated zeolite affected significantly wheat plant growth and resulted in faster growth and higher biomass production.

Dark fermentative biohydrogen production from synthetic cheese whey in an anaerobic structured-bed reactor: Performance evaluation and kinetic modeling

This study investigated the potential of dark fermentative hydrogen production from cheese whey in an acidogenic anaerobic structured-bed reactor (ASTBR). The reactor was operated at 25 °C with an organic loading rate (OLR) of 24 kg COD m−3 d−1 and a hydraulic retention time (HRT) of 24 h. The ASTBR had an average volumetric hydrogen production rate (VHPR) of 1.6 ± 0.7 L H2 L−1 d−1 and an average hydrogen yield (HY) of 1.4 ± 0.7 moL H2 mol−1 of consumed lactose. Batch experiments were performed with biomass from the ASTBR to characterize the main metabolic transformations involved in hydrogen production. An ADM1-based, unstructured kinetic model was developed and fitted to the obtained temporal profiles. The model allowed a satisfactory description of the process and indicated that hydrogen production from lactate and acetate could explain 74.5% of the total hydrogen volume produced. Because lactic-acid-producing bacteria are expected to ultimately colonize acidogenic reactors treating cheese whey, optimizing electron flow through this pathway may be a suitable strategy to enable long-term hydrogen production from cheese whey using mixed-culture fermentation.

Zeolite as a Potential Medium for Ammonium Recovery and Second Cheese Whey Treatment

The efficiency of natural zeolite to remove ammonium from artificial wastewater (ammonium aqueous solutions) and to treat second cheese whey was examined, aiming to recover nitrogen nutrients that can be used for further applications, such as slow-release fertilizers. Sorption experiments were performed using artificial wastewater and zeolite of different granulometries (i.e., 0.71–1.0, 1.8–2.0, 2.0–2.8, 2.8–4.0, and 4.0–5.0 mm). The granulometry of the zeolite had no significant effect on its ability to absorb ammonium. Nevertheless, smaller particles (0.71–1.0 mm) exhibited quicker NH4+-N adsorption rates of up to 93.0% in the first 10 min. Maximum ammonium removal efficiency by the zeolite was achieved at ammonium concentrations ranging from 10 to 80 mg/L. Kinetic experiments revealed that chemisorption is the mechanism behind the adsorption process of ammonium on zeolite, while the Freundlich isotherm model fitted the experimental data well. Column sorption experiments under batch operating mode were performed using artificial wastewater and second cheese whey. Column experiments with artificial wastewater showed high NH4+-N removal rates (over 96% in the first 120 min) for all granulometries and initial NH4+-N concentrations tested (200 and 5000 mg/L). Column experiments with second cheese whey revealed that natural zeolite can remove significant organic loads (up to 40%, 14.53 mg COD/g of zeolite) and NH4+-N (about 99%). For PO43−-P, the zeolite appeared to saturate after day 1 of the experiments at a removal capacity of 0.15 mg P/g of zeolite. Desorption experiments with water resulted in low NH4+-N and PO43−-P desorption rates indicating that the zeolite could be used as a substrate for slow nitrogen release in soils.

Evaluation of electro-Fenton method on cheese whey treatment: optimization through response surface methodology

Evaluation of electro-Fenton method on cheese whey treatment: optimization through response surface methodology