In the ballistic performance testing field of human body protection and armor protection, aiming at the demand of sub-caliber launch of assembled ammunition with the mass range of 4 ~ 16 g and velocity range of 280 ~ 500 m/s, establish the mathematical model of interior ballistic with MATLAB, calculate the interior ballistic state, and analyze the influence law of the change of charge parameters and projectile mass with the launch velocity. The results show that the propellant does not burn completely as the small-mass projectile leaves the barrel; under the same chamber volume, the projectile velocity increases with the charge mass, and this trend gradually decrease with the increasing chamber volume; under the same charge mass, the projectile velocity decreases with the increase of chamber volume, and this trend gradually increases with the increasing charge mass; under the same projectile mass, the projectile velocity decreases with the increase of charge mass, and this trend remains basically unchanged as the projectile mass increases. The analysis and calculation results are verified by the firing test. The results show that although there are differences between the actual charge quality and the calculation results, the obtained velocity changing trend is basically consistent with the trend revealed by the calculation results. The analysis and verification results can provide guidance for understanding the influencing factors affecting the launch of sub-caliber low-speed projectile, and have positive significance for improving the ballistic performance testing ability and promoting the development of the protection industry.