An experimental diagnostic based on a nanosecond-intensified charged coupled device camera and optical emission spectroscopy (OES) are proposed to observe the interfacial region between the plasma plume and liquid surfaces. The study is simulating the investigations of using non thermal atmospheric pressure plasma jets for the eradication of bacterial biofilms. The spatially and temporally resolved images are focused on the contact region in the open air between the nozzle of the reactor and liquid targets. The emission intensity from one discharge cycle is captured and accumulated over several cycles to investigate the formation and the kinetic evolution of the plasma during the interaction. Moreover, the emission intensity of the helium plasma jet and plasma behaviour with different samples are observed. It is found that some dynamic of the plasma discharge has changed during the interaction with different targets. The method can be used to improve the need of understanding of the physics and chemistry of plasma jet during the interaction with liquid surfaces. Different types of species that arrived to the samples were studied and compared with the free jet mode using OES.