Chao Phraya Research Articles

An eDNA detection of captive-bred Mekong Giant Catfish in the Chao Phraya River basin for further environmental impacts assessment

The Mekong Giant Catfish, Pangasianodon gigas, is of great cultural importance for people in Southeast Asia. Over the past century, the population of the species has dramatically declined. Several conservation measures have been taken such as establishing laws to ban catching, conserving the known spawning ground and setting up captive breeding programmes. Interestingly, the captive-bred Mekong Giant Catfish now seems to outnumber the wild population. Detecting the presence of the species is essential but conventional approaches have several limitations. Environmental DNA (eDNA) analysis was used here to detect the Mekong Giant Catfish in the Chao Phraya River basin and reservoirs in the basin. The results from this study clearly show that the eDNA of the Mekong Giant Catfish were found concentrated in the large reservoirs. The concentration of the eDNA of the Mekong Giant Catfish was found to vary among the sites which range from around 31.0–116.8 copy numbers per ml. The Mekong Giant Catfish's eDNA was not detected in water collected from the upstream rivers of the reservoirs but found in the downstream samples. The results reflect those reports of catching the species by locals in the areas. The eDNA detection was proved here to be one of the most efficient, rapid and sensitive methods for the Mekong Giant Catfish monitoring and is now ready to be used if need be.

Spatiotemporal Flood Risk Determination and Management for a Large River Basin

The Chao Phraya River Basin (CPRB) of Thailand faces flooding almost every year. The severest flood occurred in the CPRB in 2011 with the highest property damage costs (46.5 billion USD) and the highest casualty (813 deaths). The objectives of this study were thus to (1) determine flood risk indices and categorize them into four risk zones (low, moderate, high, and very high) across 994 sub-districts in the CPRB during the six rainy months (May–October); and (2) propose specific measures for flood risk management for each of the categorized risk zones. The flood risk indices were assessed as the product of two hazard variables (flood levels and monthly cumulative precipitation) and the vulnerability variable (land uses). The findings revealed spatiotemporal variations in flood risk. Spatially, the sub-districts deemed to be in the high or very high flood risk zone were mainly located close to the Chao Phraya River (CPR), where the flood levels reached 1.1 – 4 m in depth; whereas the sub-districts detected in the low or moderate flood risk zone were located further away from the CPR. Temporally, more sub-districts were detected in the high or very high risk zone in September when heavy rainfalls were observed. Specific measures are proposed herein to manage flood risk regarding the categorized zones during three periods. The preventive and mitigation measures should be prepared before flooding; emergency responses should be practically implemented during flooding; and the recovery after flooding should cover both infrastructural and environmental damage and mental/physical illnesses amongst the affected people. Intensive measures are recommended for the sub-districts located in both the high and very high risk zones. These measures may be properly loosened for the sub-districts located in the low and moderate risk zones.

First Report on Nucleolar Organizer Regions (NORs) Polymorphism and Constitutive Heterochromatin of Moonlight Gourami, Trichopodus microlepis (Perciformes, Osphronemidae)

Nucleolar organizer regions (NORs) polymorphism, constitutive heterochromatin and chromosomal analysis of Moonlight gourami, Trichopodus microlepis in Thailand were firstly reported. Specimens were collected from the Chao Phraya and Mekong Basins, Thailand. The mitotic chromosomes were directly prepared from kidney tissues of ten males and ten females. Conventional staining, Ag-NOR banding and C- banding techniques were applied to stain the chromosomes. The results shown that the diploid chromosome number of T. microlepis was 2n=46 and the fundamental number (NF) was 46 in both males and females. The karyotype consisted of 46 telocentric chromosomes classifying as 14 large and 32 medium. No heteromorphic sex chromosome was observed in T. microlepis. The results also exhibited that the interstitial nucleolar organizer regions (NORs) were clearly observed at the long arm of the chromosome pair 7. This is the first report on NORs polymorphism in T. microlepis that a heteromorphic NOR type in one female had a single NOR-bearing chromosome of the chromosome pair 7, whereas 10 males and nine females had two NOR-bearing chromosomes of the chromosome pair 7 with a homomorphic NOR type. Constitutive heterochromatins located at all centromeres of all chromosome pairs. The karyotype formula of T. microlepis is 2n (46) = Lt14 + Mt32.

Effects of Dam Construction in the Wang River on Sediment Regimes in the Chao Phraya River Basin

The Wang River is one of the major tributaries of the Chao Phraya River (CPR) system in Thailand as the key riverine sediment source supplying the Chao Phraya Delta that has experienced severe shoreline retreat in the past six decades. Historical and observed river flow and sediment data measured during 1929–2019 were used to assess the variation in total sediment load along the Wang River and evaluate the effects of three major dam constructions on sediment supplied from the Wang River to the CPR. Results indicated that sediment loads increased toward downstream. Variation in long-term total sediment load (TSL) along the river suggested that construction of the Kiew Lom Dam in 1972 did not cause a reduction in sediment yield in the Wang River Basin because it impounded less than 20% of the average annual runoff, while the Mae Chang and Kiew Koh Ma Dams caused downstream sediment reduction. These three dams are located in the upper and middle river basins, and their effects on sediment load in the Wang River are ameliorated by additional sediment supplied from the lower basin. Results confirmed that construction of these three major dams in the Wang River did not greatly impact sediment supply from the Wang River to the CPR system. The dam site and sediment load variation along the river are the primary factors controlling the impact of the dam construction.

The metal age of Thailand and Ricardo's Law of Comparative Advantage

After several decades of archaeologists interpreting Thailand's metal age development using top-down approaches drawn from 1980s archaeological theory, it has become evident they do not work for this region. When the metal assemblages from Ban Chiang and related sites in northeast Thailand were studied with a systematic methodology, alternative perspectives emerged for how to better interpret the resilient, peaceful prehistoric societies dating from approximately 2100 BCE – 300 CE. A testable model is proposed applying “Ricardo's Law of Comparative Advantage” that focuses on “regional exchange systems.” Exchange networks existed in the middle Mekong and Chao Phraya basins from the pre-metal period through the metal age and underpinned heterarchical political and economic systems whereby goods and valuables were exchanged among decentralized networks of communities.

Do non‐native ornamental fishes pose a similar level of invasion risk in neighbouring regions of similar current and future climate? Implications for conservation and management

Abstract Global trade in non‐native ornamental species coupled with high connectivity among countries is well known to result in worldwide biological invasions, which pose challenges for the conservation and management of biodiversity. There are few studies aimed at implementing management strategies that have examined differences in the potential invasiveness of non‐native species between neighbouring political regions within the same ecoregion. To compare the potential risk of invasiveness of non‐native ornamental fishes with high commercial value in the river basins of two neighbouring regions of East and Southeast Asia, 32 extant and horizon species were screened with the aquatic species invasiveness screening kit (as‐isk) for the lower Pearl River basin (South China) and the Chao Phraya River basin (Thailand). Both regional (i.e. basin level) and combined risk‐ranking thresholds were determined by receiver operating characteristic curve analysis. Of the 32 species screened, 14 were categorized as posing a high risk and seven were categorized as posing a medium risk of being invasive in both regions, under current and future climate conditions. These species have a history of invasiveness and the climate of their native ranges is similar to that of the two study regions. Pterygoplichthys pardalis received the highest risk score in both regions. The risk ranks of 11 species differed between the two regions, based on either the combined or regional thresholds, and this was partly related to the different risk of invasiveness between the two regions, coupled with cognitive subjectivity among the assessors. The results of the present study suggest that the invasion of non‐native ornamental fish species could pose similar threats to biodiversity in neighbouring regions. This will serve to inform policy makers of neighbouring countries in the development of coordinated, mutually beneficial regulations and management strategies to enhance the conservation of native species.

Detection of potentially pathogenic Arcobacter spp. in Bangkok canals and the Chao Phraya River.

The management of pathogenic bacteria in waterways is a public health issue. Here, we investigated the concentrations of potentially pathogenic bacteria, Arcobacter spp. and Campylobacter spp., and Escherichia coli, by quantifying species-specific genes in surface water samples from canals and the Chao Phraya River from June 2017 to June 2018 in Bangkok, Thailand. We assessed the relationship between the specific bacterial concentrations, water quality, and seasonal changes. Arcobacter spp. were detected at high density in all samples and showed seasonal fluctuations according to analyses based on 16S rDNA and the invasion gene ciaB. High levels of 16S rDNA and dut gene of E. coli were detected in the polluted drainage canals. A high correlation was observed between E. coli and chemical and biochemical oxygen demand (COD and BOD), suggesting that untreated domestic wastewater was the source of the E. coli. In contrast, Arcobacter spp. were detected with high density even in water samples with relatively low COD, suggesting that Arcobacter spp. are more likely than E. coli to survive in the water environment. The analysis of 16S rDNA and ciaB gene sequence analyses indicated that the Arcobacter spp. isolated from the drainage canals were A. butzleri and A. cryaerophilus.

Projecting Relative Sea Level Rise under Climate Change at the Phrachula Chomklao Fort Tide Gauge in the Upper Gulf of Thailand

This study aims to project future sea-level rise (SLR) at the Phrachula Chomklao Fort (PCF) tide gauge station in the Upper Gulf of Thailand (UGoT) using the outputs of 35 climate models under two greenhouse gas concentration scenarios: representative concentration pathway 4.5 (RCP4.5) and RCP8.5. The Linear Scaling method was found to be better than Variance Scaling and Quantile Mapping methods for removing biases in raw Global Circulation Models (GCMs) sea level data. Land subsidence, induced by excessive groundwater abstraction, was found to contribute significantly to SLR during the observed period the PCF gauging station; hence, the effects of land subsidence had to be removed from relative sea level before bias correction. The overall increase in SLR is projected to be 0.94–1.05 mm/year under RCP4.5 and 1.07–1.18 mm/year under RCP8.5 for the twenty-first century in the UGoT. The results suggest that future SLR due to climate change will not be as severe in the study region compared to average global projections. However, land subsidence can amplify future SLR. It is therefore important to regulate groundwater abstraction in the future so that SLR can be restricted. It is even more relevant in the UGoT as the raw water intake from the Chao Phraya River for municipal water supply to Bangkok is close to the estuary, and SLR in the future can pose additional challenges for the water utility.

Integrating Strategic Environmental Assessment to Climate Change Adaption in the Chao Phraya River Basin: Case Study Flood Management Plans in Ayutthaya

Traditionally, flood management has concentrated on providing protection against floods using technical measures, but there is currently an international shift towards a more integrated system of flood risk management, whereby flood risk is defined as the probability of flooding multiplied by the potential consequences. Climate change is a great challenge to sustainable development and the Millennium Development Goals (MDGs) in Thailand. The main purpose of this paper is to highlight the challenges associated with the current situation and projected impacts of climate change on the disasters and the human environment in Thailand, to review and explore the potential of Strategic Environmental Assessment (SEA), and to propose SEA in making informed decisions relevant to the implementation of the new adaptation framework in a flood management plan. Thus, current measures on how Thailand is responding to the recent impacts of climate change in river basin planning are presented. It is imperative that an appropriate environmental assessment tool, such as SEA be employed in making rational decisions regarding adaptation frameworks. SEA offers a structured and proactive environmental tool for integrating of climate change adaption into formulating Policies, Plans, and Programs (PPPs) among relevant sectors.

Evidence of cryptic diversity in freshwater Macrobrachium prawns from Indochinese riverine systems revealed by DNA barcode, species delimitation and phylogenetic approaches.

The diversity of Indochinese prawns in genus Macrobrachium is enormous due to the habitat diversification and broad tributary networks of two river basins: the Chao Phraya and the Mekong. Despite long-standing interest in SE-Asian decapod diversity, the subregional Macrobrachium fauna is still not yet comprehensively clarified in terms of taxonomic identification or genetic diversification. In this study, integrative taxonomic approaches including morphological examination, DNA barcoding, and molecular species delimitation were used to emphasize the broad scale systematics of Macrobrachium prawns in Indochina. Twenty-seven nominal species were successfully re-verified by traditional and molecular taxonomy. Barcode gap analysis supported broad overlapping of species boundaries. Taxonomic ambiguity of several deposited samples in the public database is related to inter- and intraspecific genetic divergence as indicated by BOLD discordance. Diagnostic nucleotide positions were found in six Macrobrachium species. Eighteen additional putative lineages are herein assigned using the consensus of species delimitation methods. Genetic divergence indicates the possible existence of cryptic species in four morphologically complex and wide-ranging species: M. lanchesteri, M. niphanae, M. sintangense, and some members of the M. pilimanus group. The geographical distribution of some species supports the connections and barriers attributed to paleo-historical events of SE-Asian rivers and land masses. Results of this study show explicitly the importance of freshwater ecosystems in Indochinese subregions, especially for the Mekong River Basin due to its high genetic diversity and species composition found throughout its tributaries.

Mapping biodiversity hotspots of fish communities in subtropical streams through environmental DNA

Large tropical and subtropical rivers are among the most biodiverse ecosystems worldwide, but also suffer from high anthropogenic pressures. These rivers are hitherto subject to little or no routine biomonitoring, which would be essential for identification of conservation areas of high importance. Here, we use a single environmental DNA multi-site sampling campaign across the 200,000 km2 Chao Phraya river basin, Thailand, to provide key information on fish diversity. We found a total of 108 fish taxa and identified key biodiversity patterns within the river network. By using hierarchical clustering, we grouped the fish communities of all sites across the catchment into distinct clusters. The clusters not only accurately matched the topology of the river network, but also revealed distinct groups of sites enabling informed conservation measures. Our study reveals novel opportunities of large-scale monitoring via eDNA to identify relevant areas within whole river catchments for conservation and habitat protection.

New freshwater mussels from two Southeast Asian genera Bineurus and Thaiconcha (Pseudodontini, Gonideinae, Unionidae)

The Mekong and Chao Phraya rivers harbor a species-rich freshwater mussel assemblage containing a large radiation of the Pseudodontini species. Members of the genera Bineurus Simpson 1900 and Thaiconcha Bolotov et al., 2020 primarily inhabit small and medium-sized tributaries of these rivers. Here, we present an integrative taxonomic review of these genus-level clades. We show that Bineurus contains four species: B. mouhotii (Lea, 1863), B. exilis (Morelet, 1866) stat. rev., B. anodontinum (Rochebrune, 1882) stat. rev., and B. loeiensis sp. nov. In its turn, Thaiconcha comprises three species: T. callifera (Martens, 1860), T. munelliptica sp. nov., and T. thaiensis sp. nov. Two species, Pseudodon ovalis Morlet, 1889 and P. thomsoni Morlet, 1884, are considered here as questionable taxa. These findings further highlight that Southeast Asia represents a significant evolutionary hotspot of freshwater mussels, which requires further international collaborative research and conservation efforts.

Effect of hot wind on insects in longan (Dimocarpus longan Lour.) orchard during off-season production in the Chao Phraya Delta

Effect of hot wind on insects in longan (<i>Dimocarpus longan</i> Lour.) orchard during off-season production in the Chao Phraya Delta

Consequence of Seawater Intrusion on Soil Properties in Agricultural Areas of Nonthaburi Province, Thailand

Seawater intrusion associated with climate change and sea level rise (SLR) has been postulated for the last decade that causes negative impact on worldwide environmental resources. In Thailand, however; the pertaining information to its effect on soil properties in agricultural areas has not been clearly reported yet. Hence, this study aimed to investigate the seawater intrusion effect on soil properties in agricultural areas of Nonthaburi Province, Thailand. Five study locations, which anticipated to be affected by seawater intrusion, were selected based on the different distances from the Chao Phraya River. Soil morphological properties at depth of 0-15 and 15-30 cm were recorded, besides disturbed soil samples at depth of 0-15, 15-30, 30-60, 60-90 and 90-120 cm were collected to determine the soil physical and chemical properties. Additionally, the soil electrical conductivity (ECe) and sodium adsorption ratio (SAR) analyses demonstrating soil salinity and sodicity were monthly conducted from March 2018 to February 2019 and their annual average values were calculated. The results revealed that all soils were deep to very deep, consisted of silty clay, clay textures, and its field soil pH varied from 6.0-8.0. Soil saturated hydraulic conductivity rating exhibited that it was very slow to moderate. ECe and SAR values of these soils ranged from 0.21-4.38 dS m-1 and 8.29-41.89 consecutively, which trended to increase from its annual average during March and April 2019 as well as July and August 2019. These results suggested that seawater intrusion could be presumptively regarded as a co-factor affecting the variability of soil salinity and sodicity occurring at the interval of insufficient freshwater content for forcing seawater. The implementation strategies for freshwater management and planning are required to prevent future potential seawater intrusion.

Intensification of rice cultivation in the floodplain of the Chao Phraya delta

Intensification of rice cultivation in the floodplain of the Chao Phraya delta

Abundance and characteristics of microplastics in freshwater and treated tap water in Bangkok, Thailand.

Thailand is one of the largest contributors to plastic waste in the oceans. Since mismanaged plastic waste can enter the ocean via inland waterways, microplastic contamination in freshwater used for tap water production has been raised as a potential issue. This study was conducted to determine the abundance and characteristics of microplastics in freshwater and treated tap water in Bangkok. Freshwater and treated water were collected from the eastern and western water supply systems in the dry and wet seasons. The source waters of the eastern and western water supply systems are the Chao Phraya River and Maeklong River, respectively. Approximately 0.40-2.40 particles/L microplastics was determined in the freshwater samples. No significant differences in microplastic abundance with respect to water supply system or season were found. Approximately 82.1% of the total microplastics found in the freshwater were smaller than 300µm in size. The percentage contribution of various microplastic size ranges decreased with increasing size. The majority of microplastics found in freshwater were fragments (90.2%), colorless (64.3%), and made of polyethylene polymer (45.1%). Only the eastern water supply system was capable of removing microplastic particles (27.7%) from freshwater. The identification of microplastics larger than 100µm in size in the treated tap water indicated potential contamination from microplastics in the treatment system, particularly after the sand filtration unit. The major characteristics of the microplastics found in treated tap water were similar to those found in freshwater, with 85.6% fragments, 85.1% colorless, and 47.9% made of polyethylene polymer.

Daily flow simulation in Thailand Part II: Unraveling effects of reservoir operation

Study regionUpper region of the Greater Chao Phraya River (GCPR) basin in Thailand. Study focusThe upper GCPR basin is highly regulated by multipurpose reservoirs, which obviously have altered the natural streamflow. Understanding quantitative effects of such alteration is crucial for effective water resource management. Therefore, this study aims to assess how reservoir operation affects the water balance, daily flow regime and extreme flows in this basin. For this purpose, we reconstructed streamflow in the naturalized (no reservoir) and baseline operation scenarios using the (∼1 km resolution) distributed model. To overcome data scarcity, we ran the model with global data and parameterization. A target storage-and-release-based reservoir operation module was applied in the baseline operation scenario. The model results were analyzed in comparison to observations in a wet year, a dry year, and the period 1989–2014. New hydrological insights for the regionThe reservoir operation resulted in more evaporation. It inverted the natural flow seasonality and smoothed the daily flow regime with decreasing high flows, increasing mean flows and low flows, greater baseflow contribution, and lower flashiness. It prevented or mitigated many historical extreme flow incidents. The annual flood peaks and minimum flows were markedly mitigated in terms of both magnitudes and frequencies, but their timing became more variable and difficult to predict. Altogether, the results highlighted the importance of effective decision making for real-time operation, which remain challenging in practice.

Genetic diversity of the tire track eel Mastacembelus favus in Southeast Asia inferred from microsatellite markers

The tire track eel, Mastacembelus favus, is an economically important freshwater fish in mainland Southeast Asia. Understanding the spatial population structure is important to design an effective and efficient management of the species. The aim of this study was to investigate the genetic diversity and structure of M. favus populations. In total, 519 individuals from 30 locations across its distribution range were genotyped using a set of eight polymorphic microsatellite loci. Results showed that populations in Mekong and Chao Phraya exhibited higher genetic variability than those from Gulf of Thailand and Malay–Thai Peninsula. Clustering results revealed two evolutionarily isolated groups geographically separated at Northeastern Cambodia; the more widespread group was further divided into two subclusters. The spatial genetic variation and structure of its populations are attributed to the evolutionary history and population dynamics of the species. The study provides important information for the management and conservation of M. favus in Southeast Asia.

Daily flow simulation in Thailand Part I: Testing a distributed hydrological model with seamless parameter maps based on global data

Study regionUpper region of the Greater Chao Phraya River (GCPR) basin in Thailand. Study focusThis study presents a (∼1 km resolution) distributed hydrological model, wflow_sbm, with global spatial data and parameterization for estimating daily streamflow in the upper GCPR basin, with the aim to overcome in situ data scarcity often occurring in Southeast Asia. We forced the model with the MSWEP V2 precipitation and eartH2Observe potential evapotranspiration datasets. Seamless distributed parameter maps based on pedotransfer functions (PTFs) and literature review were applied to bypass calibration. Only the KsatHorFrac parameter determining the lateral subsurface flow was calibrated. A target storage-and-release-based reservoir operation module (ROM) was implemented to simulate reservoir releases. We compared the simulated daily streamflows obtained from different PTFs and evaluated the model performance in the period 1989–2014. New hydrological insights for the regionThe global-data-driven wflow_sbm model can reconstruct daily streamflow in the upper GCPR basin, especially for natural catchments (KGE = 0.78). The ROM can capture the seasonal variability of reservoir releases, but not very accurately at the daily timescale (KGE = 0.43) since the actual reservoir operations are too complex. Different PTFs and KsatHorFrac values only introduce little uncertainty in the streamflow results. Therefore, the proposed model provides an opportunity for streamflow estimation in other ungauged or data-scarce basins in Southeast Asia. Nonetheless, the difficulty in the reservoir system modeling reflects the necessity of better understanding of human intervention on daily streamflow.

Mapping biodiversity hotspots of fish communities in subtropical streams through environmental DNA

Tropical and subtropical freshwater habitats are among the most biodiverse ecosystems worldwide, containing a characteristic fauna and high numbers of endemic species. However, exploitation of organisms, global climate change, pollution and the introduction of invasive species are severely threatening this diversity. Implementation of appropriate conservation and protection measures in tropical freshwater systems depends on comprehensive knowledge of state and change in biodiversity, which however, has been barely feasible due to logistic, technical and taxonomic challenges abound in tropical and subtropical ecosystems. Here we use a single environmental DNA (eDNA) multi-site sampling campaign distributed evenly through the 200,000 km2 Chao Phraya river basin, Thailand, to provide key information on freshwater fish diversity. We found a total of 108 fish taxa and identified key biodiversity patterns within the river network with respect to alpha- and beta-diversity patterns. By using a hierarchical clustering, we grouped the fish communities of all sites across the catchment into distinct clusters. Mapping these clusters over the catchment not only accurately matched the topology of the river network, but also revealed distinct groups of sites which should each be considered of high conservation value. Our study demonstrates a key application of large-scale monitoring (via eDNA) to identify distinct areas within a catchment for conservation and habitat protection.