Portion Of Channel Research Articles

Comparison of clinically relevant benchmarks and channel sampling methods used to assess manual cleaning compliance for flexible gastrointestinal endoscopes

The objectives of this study were to recommend sample collection method(s) based on relative soiling in patient-used gastrointestinal (GI) endoscopes and determine whether the published benchmarks for protein, bioburden, and adenosine triphosphate (ATP) remain relevant for pump-assisted manual cleaning. Patient-used gastroscopes, duodenoscopes, and colonoscopes were sampled before and after manual cleaning and assessed for protein, bioburden, and ATP levels. The biopsy port (BP) to distal end (D) sample was collected using 20 mL of sterile reverse-osmosis water. After a 200-mL flush, the umbilical (UM) to BP portion was sampled by flushing 40 mL from the UM to the D. The BP to D portion of the suction biopsy channel contained 83% of ATP residuals. Despite cleaning with brushing and a flushing pump, 25% of gastroscopes exceeded the ATP benchmark of 200 relative light units (RLU), whereas all duodenoscopes and colonoscopes had <200 RLU after cleaning. The protein and bioburden residuals after pump-assisted cleaning were consistently lower than existing benchmarks. Sampling the suction biopsy channel from BP to D detected the most residuals from patient-used GI endoscopes. The protein and bioburden benchmarks for pump-assisted cleaning can be lowered, but 200 RLU is still adequate for ATP.

Two-Way Networks: When Adaptation is Useless

Most wireless communication networks are two-way, where nodes act as both sources and destinations of messages. This allows for adaptation at or interaction between the nodes-a node's channel inputs may be functions of its message(s) and previously received signals allowing for potentially larger rates than those achievable in feedback-free one-way channels where inputs are functions of messages only. However, examples exist of channels where adaptation is not beneficial from a capacity perspective. We ask whether analogous results hold for several multiuser two-way networks. We first consider deterministic two-way channel models: the binary modulo-2 addition channel and a generalization of this, and the linear deterministic channel, which models Gaussian channels at high SNR. For these deterministic models, we obtain the capacity region for the two-way multiple access/broadcast channel (MAC/BC), the two-way Z channel, and the two-way interference channel (under certain partial adaptation constraints in some regimes). We permit all nodes to adapt their channel inputs to past outputs (except for portions of the linear high-SNR two-way interference channel where we only permit two of the four nodes to fully adapt). However, we show that the two-way fully or partially adaptive capacity region consists of two parallel one-way regions operating simultaneously in opposite directions, i.e., adaptation is useless. We next consider two noisy channel models: 1) the Gaussian two-way MAC/BC, where we show that adaptation can at most increase the sum-rate by (1/2) bit in each direction and 2) the two-way interference channel, where partial adaptation is shown to be useless when the interference is very strong. In the strong and weak interference regimes, we show that the nonadaptive Han and Kobayashi scheme utilized in parallel in both directions achieves to within a constant gap for the symmetric rate of the fully (for some regimes) or partially (for the remaining regimes) adaptive models. The central technical contribution is the derivation of new, computable outer bounds which allow for adaptation.

Pore directions for the expression of a Ca2+‐activated chloride channel

Pore directions for the expression of a Ca²⁺‐activated chloride channel

Cardioprotective Mechanism of Diazoxide Involves the Inhibition of Succinate Dehydrogenase

The adenosine triphosphate-sensitive potassium (KATP) channel opener, diazoxide, preserves myocyte volume homeostasis and contractility during stress via an unknown mechanism. Pharmacologic overlap has been suggested between succinate dehydrogenase (SDH) activity and KATP channel modulators. Diazoxide may be cardioprotective due to the inhibition of SDH which may form a portion of the mitochondrial KATP channel. To determine the role of inhibition of SDH in diazoxide's cardioprotection, this study utilized glutathione to prevent the inhibition of SDH. SDH activity was measured in isolated mitochondria exposed to succinate (control), malonate (inhibitor of succinate dehydrogenase), diazoxide, and varying concentrations of glutathione alone or in combination with diazoxide. Enzyme activity was measured by spectrophotometric analysis. To evaluate myocyte volume and contractility, cardiac myocytes were superfused with Tyrode's physiologic solution (Tyrode's) (20 minutes), followed by test solution (20 minutes), including Tyrode's, hyperkalemic cardioplegia (stress), cardioplegia + diazoxide, cardioplegia + diazoxide + glutathione, or glutathione alone; followed by Tyrode's (20 minutes). Myocyte volume and contractility were recorded using image grabbing software. Both malonate and diazoxide inhibited succinate dehydrogenase. Glutathione prevented the inhibition of succinate dehydrogenase by diazoxide in a dose-dependent manner. The addition of diazoxide prevented the detrimental myocyte swelling due to cardioplegia alone and this benefit was lost with the addition of glutathione. However, glutathione elicited an independent cardioprotective effect on myocyte contractility. The ability of diazoxide to provide beneficial myocyte homeostasis during stress involves the inhibition of succinate dehydrogenase, which may also involve the opening of a purported mitochondrial adenosine triphosphate sensitive potassium channel.

Exploring structure-function relationships between TRP and Kv channels

The molecular mechanisms underlying the activation of Transient Receptor Potential (TRP) ion channels are poorly understood when compared to those of the voltage-activated potassium (Kv) channels. The architectural and pharmacological similarities between the members of these two families of channels suggest that their structure-function relationships may have common features. We explored this hypothesis by replacing previously identified domains and critical structural motifs of the membrane-spanning portions of Kv2.1 with corresponding regions of two TRP channels, TRPM8 and TRPV1. Our results show that the S3b-S4 paddle motif of Kv2.1, but not other domains, can be replaced by the analogous regions of both TRP channels without abolishing voltage-activation. In contrast, replacement of portions of TRP channels with those of Kv2.1 consistently yielded non-functional channels. Taken together, these results suggest that most structural elements within TRP channels and Kv channels are not sufficiently related to allow for the creation of hybrid channels.

Mixing MARTINI: Electrostatic Coupling in Hybrid Atomistic–Coarse-Grained Biomolecular Simulations

Hybrid molecular dynamics simulations of atomistic (AA) solutes embedded in coarse-grained (CG) environment can substantially reduce the computational cost with respect to fully atomistic simulations. However, interfacing both levels of resolution is a major challenge that includes a balanced description of the relevant interactions. This is especially the case for polar solvents such as water, which screen the electrostatic interactions and thus require explicit electrostatic coupling between AA and CG subsystems. Here, we present and critically test computationally efficient hybrid AA/CG models. We combined the Gromos atomistic force field with the MARTINI coarse-grained force field. To enact electrostatic coupling, two recently developed CG water models with explicit electrostatic interactions were used: the polarizable MARTINI water model and the BMW model. The hybrid model was found to be sensitive to the strength of the AA-CG electrostatic coupling, which was adjusted through the relative dielectric permittivity εr(AA-CG). Potentials of mean force (PMFs) between pairs of amino acid side chain analogues in water and partitioning free enthalpies of uncharged amino acid side chain analogues between apolar solvent and water show significant differences between the hybrid simulations and the fully AA or CG simulations, in particular for charged and polar molecules. For apolar molecules, the results obtained with the hybrid AA/CG models are in better agreement with the fully atomistic results. The structures of atomistic ubiquitin solvated in CG water and of a single atomistic transmembrane α-helix and the transmembrane portion of an atomistic mechanosensitive channel in CG lipid bilayers were largely maintained during 50-100 ns of AA/CG simulations, partly due to an overstabilization of intramolecular interactions. This work highlights some key challenges on the way toward hybrid AA/CG models that are both computationally efficient and sufficiently accurate for biomolecular simulations.

Optical power and energy radiated by natural lightning

AbstractCalibrated measurements of the visible and near‐infrared radiation produced by both negative and positive cloud‐to‐ground (CG) lightning strokes have been made at distances of 5 to 32 km in southern Arizona (AZ) and the central Great Plains using a photodiode sensor with a flat spectral response between 0.4 and 1.0 µm. Time‐correlated video images (60 fps) of the channel development provided information about the types of strokes that were detected and reports from the U.S. National Lightning Detection Network indicated their locations, polarities, and estimates of their peak current. In our sample of negative strokes that were suitable for analysis, there were 23 first (or only) strokes (FS), 19 subsequent strokes that created new ground contacts (NGC), and 101 subsequent strokes that re‐illuminated a preexisting channel (PEC). We also analyzed 10 positive strokes (in nine flashes), and 73 of the larger impulses that were radiated by intracloud discharges (CPs). Assuming that these events can be approximated as isotropic sources and that the effects of atmospheric extinction are negligible, the peak optical power (Po), total optical energy (Eo), and characteristic widths of the sources (tcw = Eo/Po) have been computed. Median values of Po for negative FS, NGC, and PEC strokes were 1.8 × 1010 W, 1.1 × 1010 W, and 4.4 × 109 W, respectively. Median values of Eo were 3.6 × 106 J, 3.5 × 106 J, and 1.2 × 106 J, respectively. The median characteristic widths of negative FS, NGC, and PEC strokes were 229 µs, 244 µs, and 283 µs, respectively. Positive CG strokes produced a median Po, Eo, and tcw of 1.9 × 1010 W, 9.3 × 106 J, and 497 µs, respectively. Estimates of the space‐and‐time‐average power per unit length () in the lower portion of negative FS, NGC, and PEC channels had medians of 2.8 × 106 W/m, 3.2 × 106 W/m, and 1.4 × 106 W/m, respectively, and the median for four positive strokes was 8.8 × 106 W/m. Median values for the estimated peak electromagnetic power (PEM) radiated at early times in the strokes are 2.0 × 109 W, 2.5 × 109 W, 1.0 × 109 W, and 9.1 × 109 W for FS, NGC, PEC and positive strokes, respectively. CP events produced a median Po, Eo, and tcw of 2.0 × 109 W, 0.7 × 106 J, and 311 µs, respectively, and are in good agreement with aircraft and satellite measurements. The values of Po, Eo, and for negative CG strokes in AZ are significantly larger than prior measurements in Florida, likely because there is less atmospheric extinction in our dataset, and due to extinction, all the above values of Po, Eo, and are lower limits at the source.

Re: Video-assisted left inguinal lymphadenectomy for penile cancer

The concept of endoscopic inguinal dissection for penile cancer was proposed by Bishoff et al. from San Antonio, Texas, USA in 2003 with the dissection of 2 cadaveric models. After some modifications, the first case in clinical scenario was successfully operated at ABC Medical School, Sao Paulo, Brazil in 2003 by Tobias-Machado et al which nominate their procedure as VEIL (video endoscopic inguinal lymphadenectomy) (1). In order to achieve the maximum benefits of the minimally invasive approach, regarding mor-bidity reduction, the ABC Medical School technique was based in 10 major steps and we would like to emphasize 3 of them: distal lymphatic tissue ligation at the femoral triangle vertex, proximal control of visualized lymphatics with clips and ligation of the proximal portion of the lymphatic tissue at the deep portion of the femoral channel (2). We are concerned about these issues because in the presented video no lymphatic clipage was documented, the tissue was sectioned using only monopolar electro-cautery. This can increase the post-operative complications related to the lymphatic tissue, thereby reducing the benefits of minimally invasive procedure. It is fundamental to stress that no energy source (electric, ultrasonic, ligasure) can adequately seal lymphatic vessels. One other interesting observation is that monopolar energy can be used but control is needed when it is utilized near the skin to prevent an isquemic event. The present video demonstrates the dissection in the correct plane (under Scarpa´s fascia) to maintain some fat under the skin. A randomized comparison with open surgery in nonpalpable inguinal nodes patients showed a reduced overall complication rate of endoscopic technique (20 X 70%) specially related to skin events (0 x 40%) (3). The same number of nodes could be obtained comparing the approaches. Five year follow-up series showed cancer specific survival of 100% for negative inguinal nodes and 80% for positive nodes suggesting that VEIL is oncological effective (4). Veil is having a growing international acceptance but experience is yet small. Actually about 20 centers around the world performed the endoscopic procedure with success and very similar re-sults. Some centers are applying endoscopic surgery for other tumors as melanoma, vulvar and anal cancers. Actual results lead us to think that this technique has the potential to reduce post-operative morbidity (2-6). Nevertheless we would like to congratulate Britto et al who demonstrated the reproducibility of the method at the Federal University of Rio Grande do Norte, it could be consider especially impor-tant because VEIL can be of great value in a region which has a high incidence of penile cancer.

Validation of a Risk-Based Numerical Model for Predicting Deep-Draft Underkeel Clearance

AbstractThe channel analysis and design evaluation tool (CADET) is a numerical program to determine the optimum dredge depth for the offshore portions of entrance channels. A brief description of the theoretical basis and risk assessment in CADET is presented. Validation comparisons between CADET predictions and field measurements of wave-induced vertical motions at Ambrose Channel, New York, are presented. Prototype ship motions were measured with a differential global positioning system in April 1998 along three 488-m-long reaches. Comparisons were made with six ships, three tankers and three containerships, during inbound and outbound transits. In general, the CADET predictions matched the field measurements reasonably well, especially considering the wave heights were relatively small during the measurement period.

Operating parameters and observation modes for individual droplet analysis by inductively coupled plasma‐atomic emission spectrometry

Abstract Several operating parameters for single-droplet analysis by inductively coupled plasma‐atomic emission spectrometry were investigated and optimized. Two plasma observation modes, both of which measure the plasma side-on, were compared. In the “whole-vertical” mode, the entire vertical emission pattern of the center portion of the central channel was spatially integrated, whereas in the “lateral” mode emission from a thin horizontal slice of the vertical plasma image was measured. The limits of detection (LOD) as well as measurement precision attainable by these two observation modes were found to be practically identical. However, the lateral mode is preferred because emission is then more insensitive to a small drift in carrier-gas flow than in the vertical mode. Precision was found to degrade at carrier-gas flows that yield maximum sensitivities in both observation modes. As a result, the best precision and lowest LODs cannot be achieved under the same plasma operating conditions and a compromise is needed. In this study, precision was given a higher priority than LOD because each individual droplet is regarded as a new sample in single-droplet analysis and each such sample can be measured only once. For best precision, the observation region should be 3 mm downstream of the atomization site to avoid the adverse local plasma cooling effect of the vaporizing particle. Under optimized conditions, the best precision is about 3–4% and the absolute detection limits for eleven elements (Ag, B, Ca, Cd, Cu, Fe, Mg, Ni, Pb, Sr, and Zn) range from sub-single to hundreds of femtograms, which corresponds to 106 to 109 atoms for single-droplet analysis. In addition, a new synchronization trigger method for droplet analysis was developed. This method is based on Hα emission collected between the first and second lowest turns of the load coil. This trigger signal fires while the droplet is still intact, resides inside the lowest portion of the load coil, and is typically around 1 to 2 ms ahead of the atomization event. Jitter between this trigger signal and analyte emission is typically less than 100 μs, and is precise enough for correct timing for signal acquisition and integration.

Ultrahigh sensitive plasmonic terahertz detector based on an asymmetric dual-grating gate HEMT structure

Abstract We report on ultrahigh sensitive, broadband terahertz (THz) detectors based on asymmetric dual-grating-gate (A-DGG) high electron mobility transistors, demonstrating a record responsivity of 2.2 kV/W at 1 THz with a superior low noise equivalent power of 15 pW/√Hz using InGaAs/InAlAs/InP material systems. When THz radiation is absorbed strong THz photocurrent is first generated by the nonlinearity of the plasmon modes resonantly excited in undepleted portions of the 2D electron channel under the high-biased sub-grating of the A-DGG (as a quadratic nature of the product of local carrier density and velocity perturbations), then the THz photovoltaic response is read out at high-impedance parts of 2D channel under the other sub-grating biased at the level close to the threshold. Extraordinary enhancement by more than two orders of magnitude of the responsivity is verified with respect to that for a symmetric DGG structure.

Bax and Bcl-xL exert their regulation on different sites of the ceramide channel

The present study demonstrates the important structural features of ceramide required for proper regulation, binding and identification by both pro-apoptotic and anti-apoptotic Bcl-2 family proteins. The C-4=C-5 trans-double bond has little influence on the ability of Bax and Bcl-xL to identify and bind to these channels. The stereochemistry of the headgroup and access to the amide group of ceramide is indispensible for Bax binding, indicating that Bax may interact with the polar portion of the ceramide channel facing the bulk phase. In contrast, Bcl-xL binding to ceramide channels is tolerant of stereochemical changes in the headgroup. The present study also revealed that Bcl-xL has an optimal interaction with long-chain ceramides that are elevated early in apoptosis, whereas short-chain ceramides are not well regulated. Inhibitors specific for the hydrophobic groove of Bcl-xL, including 2-methoxyantimycin A3, ABT-737 and ABT-263 provide insights into the region of Bcl-xL involved in binding to ceramide channels. Molecular docking simulations of the lowest-energy binding poses of ceramides and Bcl-xL inhibitors to Bcl-xL were consistent with the results of our functional studies and propose potential binding modes.

The Subtype of GluN2 C-terminal Domain Determines the Response to Excitotoxic Insults

SummaryIt is currently unclear whether the GluN2 subtype influences NMDA receptor (NMDAR) excitotoxicity. We report that the toxicity of NMDAR-mediated Ca2+ influx is differentially controlled by the cytoplasmic C-terminal domains of GluN2B (CTD2B) and GluN2A (CTD2A). Studying the effects of acute expression of GluN2A/2B-based chimeric subunits with reciprocal exchanges of their CTDs revealed that CTD2B enhances NMDAR toxicity, compared to CTD2A. Furthermore, the vulnerability of forebrain neurons in vitro and in vivo to NMDAR-dependent Ca2+ influx is lowered by replacing the CTD of GluN2B with that of GluN2A by targeted exon exchange in a mouse knockin model. Mechanistically, CTD2B exhibits stronger physical/functional coupling to the PSD-95-nNOS pathway, which suppresses protective CREB activation. Dependence of NMDAR excitotoxicity on the GluN2 CTD subtype can be overcome by inducing high levels of NMDAR activity. Thus, the identity (2A versus 2B) of the GluN2 CTD controls the toxicity dose-response to episodes of NMDAR activity.

Assessing Stability of CERES-FM3 Daytime Longwave Unfiltered Radiance with AIRS Radiances

Abstract Clouds and the Earth’s Radiant Energy System (CERES) daytime longwave (LW) radiances are determined from the difference between a total (TOT) channel (0.3–200 μm) measurement and a shortwave (SW) channel (0.3–5 μm) measurement, while nighttime LW radiances are obtained directly from the TOT channel. This means that a drift in the SW channel or the SW portion of the TOT channel could impact the daytime longwave radiances, but not the nighttime ones. This study evaluates daytime and nighttime CERES LW radiances for a possible secular drift in CERES LW observations using spectral radiances observed by Atmospheric Infrared Sounder (AIRS). By examining the coincidental AIRS and CERES Flight Model 3 (FM3) measurements over the tropical clear-sky oceans for all of January and July months since 2005, a secular drift of about −0.11% yr−1 in the daytime CERES-FM3 longwave unfiltered radiance can be identified in the CERES Single Scanner Footprint (SSF) Edition 2 product. This provides an upper-bound estimation for the drift in daytime outgoing longwave radiation, which is approximately −0.323 W m−2 yr−1. This estimation is consistent with the independent assessment concluded by the CERES calibration team. Such secular drift has been greatly reduced in the latest CERES SSF Edition 3 product. Comparisons are conducted for the CERES window channel as well, and it shows essentially no drift. This study serves as a practical example illustrating how the measurements of spectrally resolved radiances can be used to help evaluate data products from other narrowband or broadband measurements.

An experimental test and models of drift and dispersal processes of pallid sturgeon (Scaphirhynchus albus) free embryos in the Missouri River

Free embryos of wild pallid sturgeon Scaphirhynchus albus were released in the Missouri River and captured at downstream sites through a 180-km reach of the river to examine ontogenetic drift and dispersal processes. Free embryos drifted primarily in the fastest portion of the river channel, and initial drift velocities for all age groups (mean = 0.66–0.70 m s−1) were only slightly slower than mean water column velocity (0.72 m s−1). During the multi-day long-distance drift period, drift velocities of all age groups declined an average of 9.7% day−1. Younger free embryos remained in the drift upon termination of the study; whereas, older age groups transitioned from drifting to settling during the study. Models based on growth of free embryos, drift behavior, size-related variations in drift rates, and channel hydraulic characteristics were developed to estimate cumulative distance drifted during ontogenetic development through a range of simulated water temperatures and velocity conditions. Those models indicated that the average free embryo would be expected to drift several hundred km during ontogenetic development. Empirical data and model results highlight the long-duration, long-distance drift and dispersal processes for pallid sturgeon early life stages. In addition, results provide a likely mechanism for lack of pallid sturgeon recruitment in fragmented river reaches where dams and reservoirs reduce the length of free-flowing river available for pallid sturgeon free embryos during ontogenetic development.

A Functional Nuclear Localization Sequence in the C. elegans TRPV Channel OCR-2

The ability to modulate gene expression in response to sensory experience is critical to the normal development and function of the nervous system. Calcium is a key activator of the signal transduction cascades that mediate the process of translating a cellular stimulus into transcriptional changes. With the recent discovery that the mammalian Cav1.2 calcium channel can be cleaved, enter the nucleus and act as a transcription factor to control neuronal gene expression, a more direct role for the calcium channels themselves in regulating transcription has begun to be appreciated. Here we report the identification of a nuclear localization sequence (NLS) in the C. elegans transient receptor potential vanilloid (TRPV) cation channel OCR-2. TRPV channels have previously been implicated in transcriptional regulation of neuronal genes in the nematode, although the precise mechanism remains unclear. We show that the NLS in OCR-2 is functional, being able to direct nuclear accumulation of a synthetic cargo protein as well as the carboxy-terminal cytosolic tail of OCR-2 where it is endogenously found. Furthermore, we discovered that a carboxy-terminal portion of the full-length channel can localize to the nucleus of neuronal cells. These results suggest that the OCR-2 TRPV cation channel may have a direct nuclear function in neuronal cells that was not previously appreciated.

A wideband multirate FFT spectrometer with highly uniform response

An integrating spectrometer based on a two-stage polyphase digital filter bank (PDFB) algorithm is described. The first PDFB operates on a time multiplexed wideband signal, with a bandwidth up to 1 GHz. The second PDFB is performed in parallel over couples of overlapping channels from the first filterbank. The design automatically deletes the unused portions of the first filterbank channels, providing a seamless, uniform channelization of the input band. The design has been implemented and tested on the VLBI DBBC platform, using a single Xilinx XC5VLX220 FPGA.

Stratigraphic, sedimentologic, and dendrogeomorphic analyses of rapid floodplain formation along the Rio Grande in Big Bend National Park, Texas

The channel of the lower Rio Grande in the Big Bend region rapidly narrows during years of low mean and peak flow. We conducted stratigraphic, sedimentologic, and dendrogeomorphic analyses within two long floodplain trenches to precisely reconstruct the timing and processes of recent floodplain formation. We show that the channel of the Rio Grande narrowed through the oblique and vertical accretion of inset floodplains following channel-widening floods in 1978 and 1990–1991. Vertical accretion occurred at high rates, ranging from 16 to 35 cm/yr. Dendrogeomorphic analyses show that the onset of channel narrowing occurred during low-flow years when channel bars obliquely and vertically accreted fine sediment. This initial stage of accretion occurred by both bed-load and suspended-load deposition within the active channel. Vegetation became established on top of these fine-grained deposits during years of low peak flow and stabilized these developing surfaces. Subsequent deposition by moderate floods (between 1.5 and 7 yr recurrence intervals) caused additional accretion at rapid rates. Suspended-sediment deposition was dominant in the upper deposits, resulting in the formation of natural levees at the channel margins and the deposition of horizontally bedded, fining-upward deposits in the floodplain trough. Overall, channel narrowing and floodplain formation occurred through an evolution from active-channel to floodplain depositional processes. High-resolution dendrogeomorphic analyses provide the ability to specifically correlate the flow record to the onset of narrowing, the establishment of riparian vegetation, the formation of natural levees, and ultimately, the conversion of portions of the active channel to floodplains.

An improved serpentine flow field with enhanced cross-flow for fuel cell applications

The paper describes a flow field design which is based on the improvement of the local cross-flow conditions in a split serpentine flow field. The layout of the flow field is such that the cross-flow is higher in the oxygen-depleted portion of the adjacent serpentine channel in a split serpentine flow field with more than one serpentine channels. The present arrangement offers the quadruple advantage of uniform reactant distribution over the entire cell active area; low overall pressure drop, thereby reducing the parasitic power losses; effective liquid water evacuation in the U-bends; and more oxygen replenishment in the oxygen-deficient portions of the serpentine channel. Computational fluid dynamics (CFD) and experimental analysis carried out on the proposed design with three serpentine channels confirm the benefits.

Pleistocene trace fossils in the Ironshore Formation, Little Cayman, British West Indies

Abstract Oosparite grainstones of the Pleistocene Ironshore Formation, Little Cayman, British West Indies, contain the trace fossils Conichnus conicus, Ophiomorpha nodosa, and Planolites beverleyensis. The dominance of vertically-oriented trace fossils, complexity of cross-stratifications, coarseness of the sediment channel fill, and presence of several rudstone layers suggest deposition close to the seaward portion of lagoonal channels where higher energy conditions prevailed.