Changes In Physiological Indices Research Articles

Impact of season and maturity stage on biochemical parameters of Averrhoa carambola fruits

The nutritional quality of fruits can be affected by changes in physiological indicators during fruit ripening, which is why it is important to identify the stage of maturity and the appropriate season for harvesting. Based on this observation, this study was carried out to evaluate the influence of the two different seasons and the stage of maturity on the nutritional parameters of Averrhoa Carambola. The results showed a significant effect (p<0.0001) of the two seasons and maturity stage on the biochemical parameters of A. carambola fruits. Ash contents and pH were significantly high in rainy seasons and at the ripe stage. Their values ​​were respectively (6.80±0.59%) and (3.54±0.27). Moisture and titratable acidity concentrations decreased significantly during maturation while total sugars, reducing and non-reducing sugars, and total soluble solids increased significantly during maturation. vitamin C and phenolic compounds were more abundant in the dry season and at the green stage. The values ​​were Vitamin C (15,869.64±0.03 mg/g), polyphenols (30.92±0.03 mg GAE/mg), flavonoids (30.59±0.28 mg EQ/g) and tannins (21-.02±0.01 mg GAE/g). This study suggests that there are differences in the biochemical parameters of starfruit between seasons depending on the maturity stage. The data provided useful information on the season and ideal stage of maturity for harvesting and their use in food products.

Expression and Functional Identification of SPL6/7/9 Genes under Drought Stress in Sugarbeet Seedlings.

Sugar beet is a significant sugar crop in China, primarily cultivated in arid regions of the north. However, drought often affects sugar beet cultivation, leading to reduced yield and quality. Therefore, understanding the impact of drought on sugar beets and studying their drought tolerance is crucial. Previous research has examined the role of SPL (SQUAMOSA promoter-binding protein-like) transcription factors in plant stress response; however, the precise contribution of SPLs to the drought stress response in sugar beets has yet to be elucidated. In this study, we identified and examined the BvSPL6, BvSPL7, and BvSPL9 genes in sugar beets, investigating their performance during the seedling stage under drought stress. We explored their drought resistance characteristics using bioinformatics, quantitative analysis, physiological experiments, and molecular biology experiments. Drought stress and rehydration treatments were applied to sugar beet seedlings, and the expression levels of BvSPL6, BvSPL7, and BvSPL9 genes in leaves were quantitatively analyzed at 11 different time points to evaluate sugar beets' response and tolerance to drought stress. Results indicated that the expression level of the BvSPL6/9 genes in leaves was upregulated during the mid-stage of drought stress and downregulated during the early and late stages. Additionally, the expression level of the BvSPL7 gene gradually increased with the duration of drought stress. Through analyzing changes in physiological indicators during different time periods of drought stress and rehydration treatment, we speculated that the regulation of BvSPL6/7/9 genes is associated with sugar beet drought resistance and their participation in drought stress response. Furthermore, we cloned the CDS sequences of BvSPL6, BvSPL7, and BvSPL9 genes from sugar beets and conducted sequence alignment with the database to validate the results. Subsequently, we constructed overexpression vectors, named 35S::BvSPL6, 35S::BvSPL7, and 35S::BvSPL9, and introduced them into sugar beets using Agrobacterium-mediated methods. Real-time fluorescence quantitative analysis revealed that the expression levels of BvSPL6/7/9 genes in transgenic sugar beets increased by 40% to 80%. The drought resistance of transgenic sugar beets was significantly enhanced compared with the control group.

Comparative transcriptomic profiles of Paulownia catalpifolia under different degrees of chilling stress during the seedling stage

BackgroundPaulownia, an ecologically and economically valuable plant species native to China, is notable for its excellent timber quality and strong adaptability. Among them, Paulownia catalpifolia displays the ability to survive in cold climate, a trait associated with northern China. Yet, the molecular information for its cold-tolerance has not been explored. This study was to investigate the changes in physiological indices and transcript levels of P. catalpifolia following cold exposure, which could provide evidence for revealing whether there were differences in the genetic basis of inducing physiological perturbations between moderate low temperature (MLT) and extreme low temperature (ELT).ResultsThe detection of physiological indices under diverse degrees of chilling stress showed similar patterns of alteration. Enhanced accumulation of osmoregulatory substances, such as soluble sugar and soluble protein, were more conducive under ELT compared to MLT in P. catalpifolia. Moreover, we observed leaf wilting symptoms distinctly after exposure to ELT for 48 h, while this effect was not obvious after MLT exposure for 48 h. Comparative transcriptomic analysis between MLT and ELT demonstrated 13,688 differentially expressed genes (DEGs), most of them appeared after 12 h and 48 h of treatment. GO and KEGG analyses elucidated prominent enrichment in aromatic-L-amino-acid decarboxylase activity term and carbohydrate metabolism pathways. Therefore, it was speculated that the DEGs involved in the above processes might be related to the difference in the contents of soluble protein and soluble sugar between MLT and ELT. Time series clustering analyses further highlighted several key genes engaged in the ‘Glycosyltransferases’, ‘Galactose metabolism’ and ‘Starch and sucrose metabolism’ pathways as well as the ‘tyrosine decarboxylase activity’ term. For instance, cellulose synthase-like A (CLSA2/9), raffinose synthase (RafS2), β-amylase (BAM1) and tyrosine/DOPA decarboxylase (TYDC1/2/5) genes, diverging in their expression trends between MLT and ELT, might significantly affect the soluble sugar and soluble protein abundance within P. catalpifolia.ConclusionBetween MLT and ELT treatments, partial overlaps in response pathways of P. catalpifolia were identified, while several genes regulating the accumulation of osmotic adjustment substances had disparate expression patterns. These findings could provide a novel physiological and molecular perspective for P. catalpifolia to adapt to complex low temperature habitats.

The Role of Liriodendron Dof Gene Family in Abiotic Stress Response.

The DOF (DNA-binding with one finger) transcription factors are exclusive to plants and play crucial roles in plant growth, development, and environmental adaptation. Although extensive research has been conducted on the Dof gene family in Arabidopsis, maize, and Solanum, investigations concerning the role of this gene family in Liriodendron remain unreported, leaving its biological function largely unknown. In this study, we performed a comprehensive genome-wide identification of the Dof gene family based on the Liriodendron genome, resulting in the discovery of a total of 17 LcDof gene members. Based on the results of phylogenetic analysis, the 17 LcDof proteins were classified into eight subfamilies. The motif analysis revealed the diverse nature of motifs within the D1 subfamily, which includes a distinct type of Dof transcription factor known as CDF (Cycling Dof Factor). We further characterized the chromosomal distribution, gene structure, conserved protein motifs, and cis-elements in the promoter regions. Additionally, utilizing transcriptome data from Liriodendron hybrids and conducting RT-qPCR experiments, we investigated the expression patterns of LhDofs under various abiotic stresses such as drought, cold, and heat stress. Notably, we found that several LhDofs, particularly LhDof4 and LhDof6, were significantly upregulated in response to abiotic stress. Furthermore, we cloned LhDof4 and LhDof6 genes and found that its encoding protein was mainly located in the nucleus by transient transformation in Liriodendron hybrids protoplast. Subsequently, we used LhDof6-overexpressing Liriodendron hybrid seedlings. We found that overexpression of LhDof6 enhanced the cold tolerance of the plants, increasing their survival rate at -20 °C. This result was further validated by changes in physiological indicators.

Physiological response of chestnuts (Castanea mollissima Blume) infected by pathogenic fungi and their correlation with fruit decay

The postharvest decay of chestnuts (Castanea mollissima Blume) limits their industrial utilization, and pathogenic fungi are the main cause for chestnut decay. In this study, the physiological changes and their correlation with the rotting degree of chestnuts were investigated during single or mixed infection by Fusarium proliferatum, Penicillium crustosum, and Alternaria alternata. During the infection period, the activities of cell wall degrading enzymes (CWDEs) and antioxidant enzymes firstly increased and then decreased, the contents of nutrients decreased, but the levels of physiological indexes increased. The mycelium and spores of pathogenic fungi colonized the intercellular space, and then the mycelium covered the entire tissue surface of chestnuts, leading to the disappearance of cell structure. Notably, the most prominent changes in physiological indexes and ultrastructure were observed in chestnuts infected with three pathogenic fungi. Furthermore, the rotting degree of chestnuts was positively correlated with the levels of CWDEs, antioxidant enzymes and malondialdehyde.

Physiological Effect of Gentle Stroking in Lambs.

The aim of the study was to determine changes in physiological indicators in lambs subjected to gentle stroking. The study included 40 three-week-old lambs (20 females of the synthetic prolific-meat (BCP) line and 20 females of the Świniarka (SW) breed). The animals were assigned to two control groups (n = 20) and two experimental groups (n = 20). Lambs from the experimental groups received treatment. Analyses consisted of determining the following parameters: heart rate (HR), saturation (S) and biochemical analysis. In the groups of sheep subjected to gentle massage, the analyses revealed a significant increase in the levels of cortisol, CAT, GST, GPx, among others, and a significant decrease in the levels of total protein, SOD, TAC, uric acid and Na+. At the same time, indicators of reduced stress levels were revealed, i.e., a significant increase in glycogen levels and a significant decrease in glucose and HR values. These results suggest that the introduction of gentle touch can induce positive states in lambs, and that the stress response may be transient and adaptive. Nevertheless, it is important to note that these interactions can be a source of stress, even if the behavioral response does not necessarily indicate stress.

Effects of Caffeine-Taurine Co-Ingestion on Endurance Cycling Performance in High Temperature and Humidity Environments.

Taurine (TAU) and caffeine (CAF), as common ergogenic aids, are known to affect exercise performance; however, the effects of their combined supplementation, particularly in high temperature and humidity environments, have not been studied. The combination of TAU and CAF will have a greater effect on endurance cycle performance and improve changes in physiological indicators during exercise compared with TAU or CAF supplementation alone and placebo. Single-blind crossover randomized controlled study. Level 1. Twelve university students majoring in physical education volunteered to receive 4 different supplement ingestions: (1) placebo (maltodextrin), (2) TAU, (3) CAF, (4) TAU + CAF. After a 7-day washout period, participants completed a time to exhaustion (TTE) test in the heat (35°C, 65% relative humidity). All experimental groups improved TTE compared with the placebo group. Peak and mean power of countermovement jump were significantly higher in the CAF group compared with the placebo group before the exhaustion exercise (P = 0.02, d = 1.2 and P = 0.04, d = 1.1, respectively). Blood lactate was significantly lower after the exhaustion test in the TAU group compared with the CAF (P < 0.01, d = 0.8) and TAU + CAF (P < 0.01, d = 0.7) groups. Core temperature in the TAU group was significantly reduced in the placebo group later in the exhaustion test (P < 0.01, d = 1.9). In high temperature and humidity environments, acute TAU, CAF, and combined supplementation all improved TTE and did not affect recovery from lower limb neuromuscular fatigue compared with placebo, with TAU having the best effect. Combined supplementation failed to exhibit superimposed performance. The results provide suggestions for the effects of TAU, CAF, and their combined intake on exercise performance in high temperature and humidity environments.

Calcium deficiency leads to fruit blackheart formation by disrupting glycometabolism and phenylpropanoid metabolism in wax gourd

Wax gourd (Benincasa hispida (Thunb.) Cogn) is a vegetable crop with a long storage period; however, blackheart disorder seriously compromises its storability and economic value. This study delves into the spatial-temporal process of blackheart disorder in wax gourd fruit at the physiological, transcriptional, and metabolic levels. The findings revealed decreased concentrations of total calcium (Ca), Ca-pectinate, and chelate-soluble pectin (CSP), and increased levels of total phenolics, flavonoids, and lignin during the development of blackheart disorder. These changes in physiological indicators in blackheart fruit were regulated by the expression of relevant genes, the accumulation of key metabolites, and increased enzyme activities in the glycometabolism and phenylpropane metabolism pathways. Moreover, transcriptome analysis revealed significant suppression in the expression of Ca channel and transport-related genes in blackheart fruit. Finally, there was a pronounced correlation between CSP, Ca-pectinate, phenolic acid, flavonoids, and monosaccharides. Weighted gene co-expression network analysis identified several transcription factors related to blackheart development were identified. Importantly, it has been discovered for the first time that the development of blackheart disorder in wax gourd results from pectin degradation and metabolic disturbance caused by Ca deficiency. These findings lay a theoretical foundation for devising strategies to manage and control blackheart in wax gourds.

Changes in Physiological Indices, Amino Acids, and Volatile Compounds in Vitis vinifera L. cv. Pinot Noir under UV-B Radiation and Water Deficit Conditions.

UV-B radiation and water deficit can challenge Pinot noir growth and fruit quality. The aim of this work is to determine the effects of UV-B and water deficit on the physiological indices, amino acids, and volatile compounds of Pinot noir vine and fruit. The results showed that both individual and combined treatments caused a decrease in the leaf SPAD, with the largest amplitude being observed in the combined treatment. Water deficit also decreased the leaf water potential and increased the juice δ13C‱ at harvest, which was the opposite of the latter under UV-B radiation. Interestingly, most of the physiological indices under combined stresses did not show significant changes compared with that under no UV-B and the well-watered control treatment. Moreover, the concentrations of amino acids and volatile compounds in the berries were determined at harvest. The amino acid contents were significantly increased by the combined treatment, particularly proline (Pro), aspartate (Arg), alanine (Ala), and threonine (Thr). There were slight increases in volatile compounds. This research substantially contributed to improve our scientific understanding of UV-B and water deficit responses in an important commercial species. In addition, it highlighted some future research to produce high-quality wines with the anticipated specific characteristics.

Емтихан стресінің әр-түрлі соматотиптері бар білім алушылардың алаңдаушылық деңгейіне және тыныс алу көрсеткіштеріне әсері

The article studied the level of situational and personal anxiety of students with different somatotypes, as well as external indicators of breathing: respiratory rate (RR), tidal volume at rest (TVR), vital capacity (VC), inspiratory reserve volume (IRV), expiratory reserve volume (ERV) under exam stress. Currently, the health of students requires a lot of attention, because students are one of the representative groups of the population of our country. The health of the younger generation is formed under the influence of biological and social factors. The ability to maintain the resistance of one’s body to exogenous factors and to adapt to changing environmental conditions depends on the state of physical development of students, the functioning of organs and systems. Adaptation to a complex of factors characteristic of higher education in a modern pedagogical environment is a complex and multi-level socio-psycho-physiological process, accompanied by significant tension in the body’s compensatory systems. Anxiety is a mental state of a person associated with increased emotional or physical stress, a tendency to experience anxiety and fear in specific social situations. The relevance of studying the factors of anxiety and emotional stability (tolerance) is determined, first of all, by the extremity and dynamics of the subject’s current life, which leads him to stress, chronic psycho-emotional tension and fear in space and time. Homeostasis of the respiratory system is a prerequisite for its normal functioning and is ensured by a number of physiological mechanisms, among which the conditioning function of the lungs occupies a particularly important place. The change in temperature and humidity of inhaled air to the state of the body is carried out due to complex processes of heat and mass transfer in the respiratory tract. Many aspects of this process in normal conditions and especially in pathologies of the respiratory system are controversial issues. Our study showed a decrease in the adaptive and physical abilities of first-year students with different somatotypes to learning conditions, and changes in physiological indicators after exam stress. During the study, first-year students with an asthenic somatotype had very high levels of personal and situational anxiety; this increase in anxiety indicates insufficient emotional adaptation to an extreme situation. A high level of anxiety threatens a person’s mental health and contributes to the development of neurotic conditions. A high level of constant anxiety is considered as a condition leading to the development of psychosomatic pathology. The article examines the indicators of the respiratory system in first-year students during the exam: in comparison with hypersthenic students and asthenic students, normosthenic students have significantly increased hemodynamic indicators, which indicates the influence of exam stress on a significant increase in respiratory rate, which is a large factor. Degree is related to the volume of breathing and, to a lesser extent, to the breathing frequency of the subjects.

Physiological and transcriptomic analyses provide preliminary insights into the autotoxicity of Lilium brownii.

Lilium brownii F. E. Brown ex Miellez var. viridulum Baker (Longya lily) is a variety of Lilium brownii F.E. Br. ex Miellez. We used HS-SPME and GC-MS to screened the tissues of L. brownii roots, stems, bulbs, and leaves and obtained 2,4-DTBP as an autotoxic substance for subsequent analysis. 2,4-DTBP was highly autotoxic in some treatment groups. Based on changes in physiological indicators, we carried out transcriptomic analysis to investigate the mechanisms of autotoxicity of substances on L. brownii and obtained 188,505 Unigenes. GO and KEGG enrichment analyses showed that L. brownii responded differently to different concentrations and treatment times of 2,4-DTBP. We observed significant changes in genes associated with ROS, phytohormones, and MAPK signaling cascades. 2,4-DTBP affects chloroplasts, the integrity of the respiratory electron transport chain, and ribosomes, causing L. brownii autotoxicity. Our findings provide a practical genomic resource for future research on L. brownii autotoxicity and evidence for the mechanism of action of autotoxic substances.

Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis

Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated with different nitrogen levels. On combining the changes in physiological indicators, high-nitrogen treatment was found to downregulate β-1,3-glucanase, reduce the decomposition of cell wall components, downregulate three proteins involved in starch metabolism, decrease the range of the amylose content and increase the range of the amylopectin, upregulate three proteins related to the lysosomal pathway, and enhance glutelin degradation. In addition, it upregulated three proteins related to flavonoid synthesis, which enhanced the stress response ability of rice, thereby contributing to the stability of biological macromolecules. The discovery of these key DEPs provides potential targets for further control over the deterioration of crop seed storage quality.

Enterococcus faecium supplementation prevents enteritis caused by Escherichia coli in goats.

The probiotic Enterococcus faecium is a gut microbe with immunomodulatory effects, which has been widely used to prevent diarrhoea in pigs and birds. Escherichia coli is a common pathogen that causes inflammatory bowel disease in animals. The aim of this study was to investigate the protective effects of E. faecium on enteritis in goats. Forty goats were randomly divided into 4 treatment groups: control, E. faecium, E. coli, and E. faecium+ E. coli. The changes of physiological indicators and diarrhoea scoring were evaluated on days -4, -2, 0, 2, 4, 6, and 8. The pathological examination, inflammatory cytokines mRNA expression and bacterial counts in jejunum and caecum were detected on day 4 and 8. The results showed that body temperature, respiratory rate, heart rate and leukocyte counts all increased from the 2nd to the 6th day after feeding with E. coli, and the diarrhoea score was significantly increased. However, E. faecium-pretreated goats had lower body temperatures and fewer leukocytes than E. coli-treated goats on day 2, as well as decreased diarrhoea scores. E. coli treatment caused histopathological damage and morphological changes in the jejunum and caecum, while pretreatment with E. faecium significantly alleviated these injuries. E. faecium pretreatment can reduce the load of E. coli and increase the prevalence of Lactobacillus, thereby balancing the microbiota in the intestine. Furthermore, E. coli-infected goats pretreated with E. faecium showed obvious inhibition of Toll-like receptor 4, interleukin (IL)-1β, IL-6, IL-8 and tumour necrosis factor-α mRNA expression in the jejunum and caecum compared to that in the E. coli treatment group. In conclusion, the addition of E. faecium to goat feed is beneficial for improving clinical symptoms, maintaining intestinal mucosa integrity, balancing the microbiota and decreasing inflammatory responses in E. coli-induced intestinal injury.

Examining Physiological Changes during Counter-Strike: Global Offensive (CS:GO) Performance in Recreational Male Esports Players

While the training of professional sports athletes and the factors determining sports success are based on well-established scientific research, esports training and markers of esports performance are not yet fully recognized or explored. Knowledge of the psychophysiological responses of the athlete’s body to esports competition is the necessary foundation for rational training management. The aim of the present study was to evaluate physiological stress while playing Counter-Strike: Global Offensive (CS:GO). Selected cardiovascular, hormonal, and biochemical indices were monitored to assess differences in stress responses between winners and losers. Twenty-two male players participated in the study (age 22.0 ± 2.0 years, CS:GO training experience 7.0 ± 2.2 years, training load 24.6 ± 11.5 h per week). Each player played two games during the CS:GO competition. The CS:GO tournament induced an increase in heart rate (HR), systolic blood pressure, and blood cortisol levels (C), and a decrease in the nonlinear heart rate variability (HRV) index based on the fractal correlation properties, called alpha1, of detrended fluctuation analysis (DFA-alpha1). In contrast, no changes were observed in blood levels of testosterone (T) and lactate (BLa). It was found that changes in physiological indices in players while playing CS:GO did not differentiate between winners and losers. The changes in the physiological parameters recorded during play indicate that CS:GO tournaments induce significant physiological arousal and can be considered a stressor.

Transcriptomic Analysis of the Effect of Pruning on Growth, Quality, and Yield of Wuyi Rock Tea.

Pruning is an important agronomic measure in tea plantation management. In this study, we analyzed the effect of pruning on gene expression in tea leaves from a transcriptomics perspective and verified the results of a transcriptomic analysis in terms of changes in physiological indicators of tea leaves. The results showed that pruning enhanced the gene expression of nine metabolic pathways in tea leaves, including fatty acid synthesis and carbohydrate metabolism, nitrogen metabolism, protein processing in the endoplasmic reticulum, and plant hormone signal transduction, thereby promoting the growth of tea plants and increasing tea yield. However, pruning reduced the gene expression of nine metabolic pathways, including secondary metabolites biosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, and sesquiterpenoid and triterpenoid biosynthesis, and lowered the content of caffeine, flavonoids, and free amino acids in tea plant leaves. In conclusion, pruning could promote the growth of tea plants and increase the yield of tea, but it was not conducive to the accumulation of some quality indicators in tea leaves, especially caffeine, flavonoids, and free amino acids, which, in turn, reduced the quality of tea. This study provides an important theoretical reference for the management of agronomic measures in tea plantations.

Assessment of a Coordinated Arrangement of Wearable Physiological Sensors for Stress Checking in Work Spaces by Utilizing Organic Markers

The objectives of this investigation were to create and evaluate a wearable physiological sensor system for detecting human stress using ECG, EDA, and EEG data, as well as to see if the changes in physiological indicators were correlated levels. The main elements of the study are listed below. This study included 15 healthy volunteers with an average age of 40.8 years, comprising seven men and six women. They underwent the well-known Maastricht Intense Stress Test, a procedure that is known to cause significant physical and psychological stress, while wearing three commercial sensors have been employed to keep track of their physiologic responses.Throughout salivary samples were taken at various points. The use of a Salivary concentrations of cortisol were correlated with the obtained physiological indicators using an algorithm called the Support Vector Machine (SVM) segmentation technique were both part of the statistical analysis. A significant ability to discriminate between stress and relaxation was shown by fifteen features obtained from measurements including heart rate variability, electro dermal activity, and electroencephalography signals. Based on these important features, the classification system produced results with an accuracy rate of 86% that was satisfactory.Furthermore, the correlation analysis revealed, with an R-squared value of 0.714, a considerable consistency between changes in physiological characteristics and the trends in salivary cortisol levels. The study successfully showed that the wearable sensor system's implementation caught human stress levels and measured the state of stress with accuracy. The design of adaptable and controllable systems, such as medical devices, targeted at inducing interventions to stop stress-related effects, is particularly affected by these findings.

Physiological and Molecular Responses of Apocynum venetum L. (Apocynaceae) on Salt Stress

Soil salinization is a crucial factor that impacts plant distribution and growth. Apocynum venetum, an ornamental plant with medicinal value, has shown remarkable salt tolerance. However, the specific mechanisms through which A. venetum responds to salt stress are not yet fully understood. To address this gap, we conducted a study where 10-week-old A. venetum seedlings were subjected to salt stress by irrigating them with a nutrient solution containing varying concentrations of NaCl (0, 100, 200, and 350 mmol·L−1). After the salt stress treatment, various growth indicators (such as plant height, root length, root fresh weight, root dry weight, leaf fresh weight, leaf dry weight, root water content, leaf water content, and root–leaf ratio) as well as physiological indicators (including SOD and CAT activities in both leaves and roots, soluble protein contents in leaves and roots, and chlorophyll and carotene contents in leaves) were determined. In addition, the gene expression profile of roots under salt stress was examined by transcriptome sequencing to explore the mechanism of salt response in A. venetum. Our results show that salt stress led to yellowing and wilting of A. venetum seedling leaves. Furthermore, the chlorophyll and carotenoid contents in the leaves of the 350 mmol·L−1 NaCl-treated group were significantly reduced. Although the leaf and root biomass gradually decreased with an increase in the salt concentration, the root–leaf ratio exhibited a decreasing trend. NaCl stress also caused significant changes in physiological indices in the A. venntum leaves and roots. The activities of superoxide dismutase (SOD) and catalase (CAT) increased in both leaves and roots of the 100 mmol·L−1 NaCl-treated group. The soluble protein content in both leaves and roots increased under the 200 mmol·L−1 NaCl stress. To screen changes in root gene expression, transcriptome sequencing and qRT-PCR were performed. GO and KEGG enrichment analyses revealed that salt stress primarily affects carbohydrate metabolism, MAPK signaling transduction, phytohormone signaling pathways, glyoxylate and dicarboxylate metabolism, and other pathways. This study provides a novel understanding of the growth and physiological response of A. venetum leaf and root to NaCl stress, as well as the changes in the transcription levels in A. venetum root. The results serve as a reference for future research on salt-tolerant mechanisms and molecular breeding of A. venetum.

Effectiveness of technology-based interventions compared with other non-pharmacological interventions for relieving procedural pain in hospitalized neonates: a systematic review.

The objective of this review was to evaluate the effectiveness of technology-based interventions for relieving procedural pain among hospitalized neonates compared with other non-pharmacological interventions. Neonates who require hospital care often experience acute pain during medical procedures. The current best practice for relieving pain in neonates is the use of non-pharmacological interventions, such as oral solutions or intervention-based human touch. Technological solutions (eg, games, eHealth applications, mechanical vibrators) have become more commonplace in pediatric pain management over recent years. However, there is a sizeable knowledge gap concerning how effective technology-based interventions are for relieving pain in neonates. This review considered experimental trials that include technology-based, non-pharmacological interventions for relieving procedural pain among hospitalized neonates. The primary outcomes of interest include pain response to a procedure measured by a pain assessment scale validated for neonates, behavioral indicators, and changes in physiological indicators. The search strategy aimed to identify both published and unpublished studies. PubMed MEDLINE (PubMed), CINAHL (EBSCOhost), Scopus, Cochrane Central Register of Controlled Trials, MedNar, and EBSCO Open Dissertations databases were searched for studies published in English, Finnish, or Swedish. Critical appraisal and data extraction were conducted by 2 independent researchers who adhered to JBI methodology. Meta-analysis could not be performed due to considerable heterogeneity in the studies; as a result, the findings are presented narratively. A total of 10 randomized controlled trials involving 618 children were included in the review. The staff members delivering the interventions and the outcome assessors were not blinded in all of the studies, which introduced a potential risk of bias. The presented technology-based interventions were diverse, including laser acupuncture, noninvasive electrical stimulation of acupuncture points, robot platform, vibratory stimulation, recorded maternal voice, and recorded intrauterine voice. In the studies, pain was measured using validated pain scales, behavioral indicators, and physiological variables. In the studies in which pain was assessed with a validated pain measure (N=8), technology-based pain relief was significantly more effective than the comparator in 2 studies, whereas no statistically significant differences were observed in 4 studies and the technology-based intervention was less effective than the comparator in 2 studies. The effectiveness of technology-based interventions in relieving neonatal pain, either as a standalone method or in combination with another non-pharmacological method, was mixed. Further research is needed to provide reliable evidence on which technology-based, non-pharmacological pain relief intervention is most effective for hospitalized neonates. [http://links.lww.com/SRX/A19].

Promoting Heme and Phycocyanin Biosynthesis in Synechocystis sp. PCC 6803 by Overexpression of Porphyrin Pathway Genes with Genetic Engineering.

Due to their unique biochemical and spectroscopic properties, both heme and phycocyanobilin are widely applied in the medical and food industries. Synechocystis sp. PCC 6803 contains both heme and phycocyanin, and is capable of synthesizing phycocyanin using heme as a precursor. The aim of this study was to uncover viable metabolic targets in the porphyrin pathway from Synechocystis sp. PCC 6803 to promote the accumulation of heme and phycocyanin in the recombinant strains of microalgae. A total of 10 genes related to heme synthesis pathway derived from Synechococcus elongatus PCC 7942 and 12 genes related to endogenous heme synthesis were individually overexpressed in strain PCC 6803. The growth rate and pigment content (heme, phycocyanin, chlorophyll a and carotenoids) of 22 recombinant algal strains were characterized. Quantitative real-time PCR technology was used to investigate the molecular mechanisms underlying the changes in physiological indicators in the recombinant algal strains. Among the 22 mutant strains, the mutant overexpressing the haemoglobin gene (glbN) of strain PCC 6803 had the highest heme content, which was 2.5 times higher than the wild type; the mutant overexpressing the gene of strain PCC 7942 (hemF) had the highest phycocyanin content, which was 4.57 times higher than the wild type. Overall, the results suggest that genes in the porphyrin pathway could significantly affect the heme and phycocyanin content in strain PCC 6803. Our study provides novel crucial targets for promoting the accumulation of heme and phycocyanin in cyanobacteria.

Crossover point and maximal fat oxidation training effects on blood lipid metabolism in young overweight women: a pilot study.

Purpose: To determine the effects of weight reduction schemes using the exercise intensities corresponding to maximal fat oxidation (FATmax) and crossover point (COP). The effects of different intervention protocols on blood lipid metabolism were compared to explore how fat can be consumed and used more efficiently and provide a theoretical basis for weight loss through exercise. Methods: This study included 30 young overweight women randomly divided into the COP, FATmax, and control groups. Participants in the COP and FATmax groups exercised for 45min four times a week for 8weeks after the individual treadmill exercise test. The control group did not perform any exercise. Results: After 8weeks of training, participants in the COP group significantly decreased weight (2.6 ± 3.3kg), body mass index (0.91 ± 1.26kg/m2), body fat percentage (1.21% ± 1.50%), and fat mass (1.90 ± 2.30kg) (p < 0.05). They also had significantly decreased hip circumference (4.8 ± 3.3cm), serum apolipoprotein B (ApoB) levels (15.48 ± 14.19mg/dL), and ApoB/apolipoprotein AI (ApoAI) ratios (0.47 ± 0.37) (p < 0.01). However, their serum ApoAI levels were significantly increased (14.18 ± 10.24mg/dL; p < 0.01). Participants in the FATmax group had significantly decreased hip circumference (2.4 ± 2.0cm), serum ApoB levels (14.49 ± 11.00mg/dL), and ApoB/ApoAI ratios (0.59 ± 0.30) (p < 0.01) but significantly increased serum ApoAI levels (29.53 ± 13.29mg/dL; p < 0.01). No significant changes in physiological indexes were observed in participants in the control group. Conclusion: Personalised exercise intervention positively affected central obesity, effectively improving blood lipid metabolism and fat oxidation, reducing cardiovascular disease risk in young overweight women. COP training improved weight and body composition better than the FATmax exercise, while the latter provided greater improvements in serum ApoAI levels.