Changes In Skin Thickness Research Articles

Discretization of Thin-Walled Sections with Variable Wall Thickness

At the stage of designing thin-walled aircraft structures, to simplify calculations, their cross sections are idealized. To do this, the section with the skin and the longitudinal elements reinforcing it is replaced (when determining normal stresses) by a discrete one, consisting of concentrated areas at characteristic points. In this case, the equality of the moments of inertia of the initial and discrete sections is preserved. Such idealization is used in the calculation of thin-walled rods for normal and shear stresses (Wagner model). For sections consisting of a system of rectangular strips of constant thickness, discretization allows to set approximate values of normal and shear stresses and accurately find the locations of the singular points of the bending center (in an open contour) and the center of rigidity (in a closed one). The discrete model of a strip consists of three lumped areas: two at the edges and one in the center. The paper proposes to extend the discrete model to sections in which the skin thickness changes according to a linear law. In addition to the rectangular strip, it is possible to use elongated triangles and trapezoids, replaced by three and four concentrated areas, respectively. The possibility of using a discrete model for calculating some thin-walled sections of open and closed contours is considered. The section of an open contour is studied - the problem of transverse bending without torsion of a channel having flanges with a linearly variable thickness. Differences in the flows of tangential forces calculated from the exact and discrete models are shown. The coincidence of the results in determining the position of the bending center according to two models was established. When studying the application of a discrete model to a closed contour, its simplified option is proposed. The problem of transverse bending without torsion and finding the center of rigidity in a section with a contour line in the form of a trapezoid with front and rear walls of constant thickness and upper and lower skins and a similar section with a contour in the form of a rectangle was considered. Differences in the flows of tangential forces calculated by exact and discrete models are established. For a closed section in the form of a rectangle, the decrease in the moment of inertia for torsion due to the redistribution of material in the cross section was studied separately. It has been established that when finding the position of the center of rigidity, the discrepancy between the results of the exact and discrete models in sections with geometric parameters close to real ones was less than 1% for a rectangular contour, and 4% for a trapezoidal contour. The results indicate the possibility of extending the application of the discrete model of thin-walled cross section to the design calculations of thin-walled rods with variable skin thickness, representing practical structures.

Assessment of Skin by High Frequency Musculoskeletal Ultrasound in Egyptian Patients with Systemic Sclerosis

Abstract Background Fibrotic involvement of the skin is a cardinal feature of systemic sclerosis (SSc). The extent of skin involvement is associated with internal organ involvement with severe disease course and poor prognosis. SSc skin involvement is characterized by three phases in temporal sequence: edematous, fibrotic, and atrophic. A palpation-based semi- quantitative score, the modified Rodnan skin score (mRSS) is widely used for the assessment of skin involvement. It is a quick, noninvasive clinical assessment that correlates with histological grading of skin fibrosis. However, it lacks the sensitivity to differentiate between clinically indistinguishable pathological phases such as inflammatory edema vs. established fibrosis. More objective approaches to measure skin involvement imaging have been developed in the past decades.In our study, we review the use of ultrasound for the assessment of early changes in skin thickness and echogenicity in patients with SSc, focusing. mainly on high frequency ultrasound. Aim of the Work to evaluate the role of high frequency ultrasound (HFUS) of skin in assessment of early changes in skin thickness and echogenicity in patients with SSc. Patients and Methods Our study is a cross-sectional, observational study involving homogenous groups consisting of 23 SSc patients and 21 healthy age and sex matched controls. Patients who fulfilled the 2013 ACR/EULAR Classification Criteria for SSc. Results DT was evaluated by both mRSS and HFUS at 5 skin areas in both patients and control. We found statistically significant difference between the two studied groups as regards in area 1 longitudinal (P = 0.017) and area 1 in transverse (P = 0.038), tendency to significance in area 2 (P = 0.054). Subclinical dermal involvement was detected by HFUS even in the skin areas in patients who had a normal local MRSS ( These areas were not planned to be assessed by high frequency ultrasound but added to the five areas to be assessed to evaluate for ultrasound detected subclinical involvement of skin in SSc patients ). In particular, DT was highly significantly greater in patients with area with Rodnan zero score at dorsum of hand than in healthy subjects (longitudinal and transverse) (P < 0.001, 0.005 ) respectively. Similar changes at the level of echogenicity occurred in determination of the stage of the disease, with increased skin thickness and decreased echogenicity occurring early and during skin disease activity due to edema; and decrease skin thickness and increased echogenicity occurring late with skin atrophy Conclusion that high frequency ultrasound is a useful tool in assessment of skin pathologic changes (even subclinical changes) in terms of thickness and echogenicity in patients with SSc both at disease onset and during follow up of response to therapy and disease stage. Moreover, it could be a useful screening tool with definite cutoff values at certain areas that differentiate normal from pathologic skin thickness.

Observational Study of the Changes in Skin Thickness Among Children with Atopic Dermatitis Treated with Topical Mometasone Furoate 0.1% Cream

Background: Atopic dermatitis (AD) affects up to 20% of children worldwide and, as of now, it has no cure. The primary treatment for AD in children involves the use of topical corticosteroids. Objectives: Our objective was to evaluate the potential for skin atrophy caused by the application of mometasone furoate 0.1% cream in children with moderate to severe AD. Methods: This study was a single-center, prospective cohort study involving 22 children aged 6-12 years with moderate to severe AD. The children applied topical mometasone furoate 0.1% cream for 6 weeks in a non-occlusive manner. We conducted dermoscopic and ultrasonographic assessments of skin atrophy at 0, 2, and 6 weeks into the treatment. Results: All patients exhibited an improvement in skin erythema. At the beginning of the study, 91% of the patients had mild to moderate erythema, while 9.1% had severe erythema. After 6 weeks of using topical mometasone, 27.3% had mild erythema, and 72.7% had completely resolved erythema (P

Large Deflection Model and Optimal Design for a Morphing Wing Leading-Edge Skin

Morphing leading edge has great potential to enhance the flight efficiency of a vehicle due to its smooth and seamless surface. To improve the computational efficiency of leading-edge bending deformation, this paper developed a parametric method for modeling the large planar deflection of the flexible skins. Based on the chain beam constrained model, the curvature of the skin is approximated by introducing the parameter of the initial inclination angle. The correctness of the model is verified through tests and finite element analysis. Based on this model, the number and position of driving points, the magnitude of driving force, and the thickness of the skin were optimized with the accuracy of leading-edge bending deformation as the optimization target. The results show that the accuracy of leading-edge bending deformation gradually improves as the number of drive points increases and the driving force range expands. The solution’s accuracy with the driving point’s target position as the driving variable is significantly higher than that of the solution with the driving force magnitude as the variable. The change in skin thickness can provide a local improvement in accuracy for the part of the curvature that has a large variation.

Skin response to lipopolysaccharide-induced sepsis based on histology

Introduction: The skin is the largest organ of the human body. Sepsis is a serious disease and causes multi-organ damage, with a high cause of death. however, the mechanism by which sepsis can damage the skin structure is not clear. This study aimed to investigate skin damage in a lipopolysaccharide-induced endotoxemia (LPS) model widely used in mice. Methods: This study used an experimental design with a control group that was randomized solely for the post-test. White mice (Mus musculus), the study's population, made up this group. LPS injection for 4 hours (LPS+4h, n=4), LPS injection for 8 hours (LPS+8h, n=4), and control group (Ctrl group, n=4) were randomly assigned to mice. The SPSS ver. 25 statistical analysis program was used. One-Way ANOVA was used to compare more than three sets of data, and Tukey's multiple comparison test was used to assess the results. The p-value of 0.05 was used to determine if the difference was significant. Results: Based on this study, LPS injection increased the leukocyte concentration significantly (p-value<0.05) in the 4 h and 8 h vs control group. LPS-induced sepsis decreased body weight significantly (p-value<0.05). The morphology of skin thickness in the control group was normal, according to the results of the histopathologic study of the area stained with hematoxylin and eosin. However, the thickness was decreased in mice after 4 hours and 8 injections of LPS significantly (p-value<0.05). Conclusion: LPS-induced septic mice cause damage to the skin, and changes in skin thickness due to the inflammatory process due to sepsis.

High-frequency ultrasound biomicroscopy findings of the skin of dogs with atopic dermatitis.

The high-frequency ultrasonographic appearance of skin of dogs with atopic dermatitis (cAD) has not been described. To compare high-frequency ultrasonographic findings among lesional, macroscopically nonlesional skin of dogs with cAD, and the macroscopically nonlesional skin of healthy dogs. Additionally, to determine whether there is any correlation between the ultrasonographic findings in lesional skin and local Canine Atopic Dermatitis Extent and Severity Index, 4th iteration (CADESI-04) or its domains (erythema, lichenification, excoriations/alopecia). As a secondary aim, six cAD dogs were re-evaluated after management intervention. Twenty dogs with cAD (six were re-examined after treatment) and six healthy dogs. In all dogs, ultrasonographic examination was performed on the same 10 skin sites, using a 50 MHz transducer. Wrinkling of skin surface, presence/width of subepidermal low echogenic band, hypoechogenicity of dermis and thickness of the skin were evaluated and scored/measured blindly. Dermal hypoechogenicity was more common and severe in lesional compared to macroscopically nonlesional skin of dogs with cAD. In lesional skin, presence/severity of wrinkling of skin surface and of dermal hypoechogenicity were positively correlated with presence/severity of lichenification, while severity of dermal hypoechogenicity was positively correlated with local CADESI-04. A positive correlation between the change in skin thickness and the change in the severity of erythema during treatment was noted. High-frequency ultrasound biomicroscopy may be useful for the evaluation of skin of dogs with cAD and for evaluating the progression of skin lesions during treatment.

The Dynamic Changes in Skin Thickness of Forehead during Tissue Expansion.

In addition to providing extra flap size, the tissue expansion process also brings changes in flap thickness. This study aims to identify the changes in the forehead flap thickness during the tissue expansion period. Patients undergoing forehead expander embedment from September 2021 to September 2022 were included. The thickness of the forehead skin and subcutaneous tissue were measured with ultrasound before and 1, 2, 3, and 4 months after expansion. Twelve patients were included. The average expansion period was 4.6 months, and the mean expansion volume was 657.1 mL. The thickness of skin and subcutaneous tissue in the central forehead changed from 1.09 ± 0.06 to 0.63 ± 0.05 mm and from 2.53 ± 0.25 to 0.71 ± 0.09 mm, respectively. In the left frontotemporal region, skin and subcutaneous tissue thickness changed from 1.03 ± 0.05 to 0.52 ± 0.05 mm and 2.02 ± 0.21 to 0.62 ± 0.08 mm. On the right side, skin and subcutaneous tissue thickness changed from 1.01 ± 0.05 to 0.50 ± 0.04 mm and 2.06 ± 0.21 to 0.50 ± 0.05 mm. This study measured the dynamic changes in the thickness of the forehead flap during expansion. The thickness of the forehead flap decreased the fastest in the first 2 months of expansion, and the changes in skin and subcutaneous thickness slowed down in the third and fourth months and tended to a minimum value. Additionally, the thickness of subcutaneous tissue decreased greater in magnitude than the dermal tissue.

Measurement of Skin Thickness Using Ultrasonography to Test the Usefulness of Elastic Compression Stockings for Leg Edema in Pregnant Women.

Background: One of the most common treatments for leg edema during pregnancy is the use of compression stockings. The purpose of this study was to evaluate the objective effectiveness in pregnant women, by measuring the changes of skin thickness using ultrasonography. Methods: Pregnant women were diagnosed with leg edema using the pitting edema method at 36 weeks of gestation. Twenty-four pregnant women (48 legs) with leg edema spent time without wearing elastic stockings at 36–37 weeks of gestation. Then, they wore elastic stockings for one week at 37–38 weeks of gestation. We measured the grade of edema (from 0 to 3) and the skin thickness of the lower leg by portable ultrasonography at 36, 37, and 38 weeks of gestation (a before-and-after study). Results: In 24 pregnant women, thigh edema was not detected in any of the 48 legs before or after the use of elastic stockings. All 48 legs in 24 pregnant women had physiological lower leg edema, but not thigh edema. The average grade of pitting edema in each lower leg significantly decreased after using the stockings (36 weeks, 1.77 ± 0.85; 37 weeks, 1.79 ± 0.77; 38 weeks, 1.04 ± 0.74, p < 0.0001). In addition, the skin thickness of the lower legs was significantly decreased after the use of elastic stockings (36 weeks, 7.47 ± 2.45 mm; 37 weeks, 7.93 ± 2.83 mm; 38 weeks, 7.15 ± 2.35 mm, p < 0.0001). Conclusions: The wearing of elastic compression stockings on the lower legs is objectively effective for improving leg edema in pregnant women.

Shore hardness is a more representative measurement of bulk tissue biomechanics than of skin biomechanics.

To support the effective use of Shore hardness (SH) in research and clinical practice this study investigates whether SH should be interpreted as a measurement of skin or of bulk tissue biomechanics. A 3D finite element model of the heel and a validated model of a Shore-00 durometer were used to simulate testing for different combinations of stiffness and thickness in the skin and subcutaneous tissue. The results of this numerical analysis showed that SH is significantly more sensitive to changes in skin thickness, relatively to subcutaneous tissue, but equally sensitive to changes in the stiffness of either tissue. Indicatively, 25% reduction in skin thickness (0.3 mm thickness change) or in subcutaneous tissue thickness (5.9 mm thickness change), reduced SH by 7% or increased SH by 2% respectively. At the same time, 25% reduction in skin stiffness (10.1 MPa change in initial shear modulus) or of subcutaneous tissue (4.1 MPa change in initial shear modulus) led to 11% or 8% reduction in SH respectively. In the literature, SH is commonly used to study skin biomechanics. However, this analysis indicates that SH quantifies the deformability of bulk tissue, not of skin. Measurements of skin thickness are also necessary for the correct interpretation of SH.

Drug Delivery through the Psoriatic Epidermal Barrier-A "Skin-On-A-Chip" Permeability Study and Ex Vivo Optical Imaging.

Psoriasis is a chronic inflammatory disease with unmet medical needs. To clarify potential therapeutic targets, different animal models have been developed. In the current study, imiquimod-induced psoriasiform dermatitis was used for monitoring the changes in skin thickness, transepidermal water loss, body weight, blood perfusion and drug permeability for a topical cream formulation of caffeine, both in wild type and in knock out mice. Morphological characterization of control and diseased tissues was performed by scanning electron microscopy and two-photon microscopy. The chemically induced psoriatic group showed increased skin permeability for the model drug during disease progression. In wild type and TRPA1 KO mice, however, enhanced skin thickness and hyperkeratosis blocked further increase of drug penetration at the late phase (96 h). These results indicate that topical drug therapy can be more effective in early phases of plaque development, when skin thickness is lower. Although paracellular connections (tight junctions) are looser in the advanced phase, hyperkeratosis blocks drug delivery through the transappendageal routes. Novel drug formulations may have the potency for effective drug delivery across the epidermal barrier even in the advanced phase. For development of more effective topical drugs, further research is proposed to explore drug penetration both in healthy and diseased conditions.

An analysis of skin thickness in the Dezhou donkey population and identification of candidate genes by RNA-seq.

The aim of the present study was to analyze the main factors that have a significant impact on skin thickness, and to further identify the genes and signaling pathways regulating skin growth by RNA-seq in Dezhou donkeys. Skin samples from different body regions of 15 slaughtered donkeys were obtained to study variations in skin thickness over the bodies. Skin thickness data for another 514 donkeys was obtained by minimally invasive skin sampling from the back, and measurements of the donkeys' body size traits and pedigree data were also collected. These data were used to analyze changes in skin thickness and estimate genetic parameters. In addition, transcriptomic analysis was conducted on the skin tissues of individuals from two groups with significant differences in skin thickness. Our results showed that skin thickness over the bodies ranged from 1.08 to 4.36mm. The skin from the back was the thickest and had the highest correlation with that of other regions of the body. The skin thickness decreased from the back to the side of the ventral abdomen, and the skin thickness on the limbs increased from the proximal end to the distal end. The results also showed that the skin from the same body regions of jacks was thicker than that of jennies in the same age group. The skin thickness of jennies increased from birth to the age of 2 and then clearly decreased after 2years of age. The estimated heritability of skin thickness was 0.15, and the genetic correlations between skin thickness and body size traits were negligible. Transcriptome analysis showed that the thick-skin group had 65 up-regulated genes and 38 down-regulated genes compared with the thin-skin group. The differentially expressed genes were highly enriched in epidermal development and cell adhesion molecule signaling pathways. We identified the candidate genes responsible for variations in skin thickness in the Dezhou donkey, including KRT10, KRT1, CLDN9, MHCII and MMP28. These results contribute to a better understanding of the growth and development of donkey skin, reveal the molecular mechanism responsible for donkey skin thickness and suggest directions for genetic selection in the Dezhou donkey population.

Stromal vascular fraction improves the durability of autologous fat temple augmentation—A split-face randomized study using ultrasound biomicroscopy

Autologous lipotransfer aims to restore aging-associated volume loss, but with low predictability owing to 20-90% first-year loss of transferred fat. Enrichment by adipose-derived stem cells within the stromal vascular fraction (SVF) aims to improve volume retention through their differentiation potential and paracrine actions exerted by secreted trophic and angiogenic factors. Assessing studies lacked split-face designs, and used multitudes of enrichment ratios, preparation techniques and evaluation methods ending in contradictory reports regarding enrichment advantage. To test whether enriching the autologous fat graft with SVF will increase its residual volume as compared to non-enriched graft. A standardized enrichment protocol and ratio and objective assessment were employed. In a split-face design, and after random assignment, bilateral temple augmentation using non-enriched versus SVF-enriched autologous lipotransfer were compared in middle-aged females otherwise healthy non-pregnant or breast-feeding females abstaining from esthetic or weight-controlling procedures. Temple volume scale (TVS), skin layers' thickness measured by ultrasound biomicroscopy (UBM), visual analog scale for patients' satisfaction, and side effects were blindly assessed at 1 week, 3 months, and 6 months. In the included 15 females, TVS was significantly lower (0.5±0.5 versus 1.1±0.7, P=0.0001), and% hypodermal augmentation was significantly higher (70.92±58.09 versus 18.93±19.33, P=0.001) on the SVF-enriched side at 6 months. Patient satisfaction was similar bilaterally (P=1), as were sequelae frequencies as lumping, edema, and ecchymosis. SVF enrichment of transferred fat significantly improved its residual volume at 6 months; a conclusion that needs further validation. UBM was an informative objective tool for the following temple skin thickness changes. Trial registration clinical trials.gov (NCT03965936).

Effect of changes in Skin Thickness on pain-relief Transcutaneous Electrical Nerve Stimulation (TENS).

Transcutaneous Electrical Nerve Stimulation (TENS) suppresses chronic pain by stimulating deep nerves near the fascia from electrodes on the skin's surface. TENS has different effects on patients of different ages due to the variation of the thickness of skin layers when one becomes older.In this paper, we aim to optimize the stimulation effectiveness of TENS for patients of different ages through investigation of TENS stimulations of three different skin types categorized by age, Young, Old, and Older. In this investigation, the skin layer (stratum corneum, epidermis layer, dermis layer) in each model was created, and the thickness was varied. The effect of sin wave stimulation at 1 Hz, 100 Hz, and 10 kHz on the nerve stimulation effect near the fascia was examined.It is found that besides the well-known effect of stratum corneum, the thickness of the dermis layer significantly affects the stimulating effect. In addition, by using a lumped circuit model, it is showed that the change in the current path causes a mitigation in the stimulation effect in the dermis layer.

Peculiarities of age-related morphological changes in the dermal-hair cover of the Pamir yak ecotype bred in the mountain zone of Northern Tajikistan

In the process of animal adaptation to changing environmental conditions, the hair cover, which is known to perform a heat-protective function primarily, plays a significant role. The hair cover of animals undergoes changes depending on the season of the year and the natural and climatic zone in which the animals live. It has its peculiarities in different species (breeds). The nature and features of the hair cover of animals are often used by scientists to compile zoological systematics, assess the health and constitution types of animals, their acclimatisation ability, etc. In this article, the authors reviewed age-related changes in weight, size, thickness and tightness of animal skins (changes in skin thickness from the ridge to the floor) and features of the hair cover of the skin’s yaks of Northern Tajikistan. The authors also noted that in all animals, the weight of hides increases in proportion to the rise in live body weight. The absolute importance of skins, up to the age of half a year of yaks, is almost two times higher than cattle. In yaks at one- and three years old, the thick- ness of skins at the standard point in these animals is somewhat comparable. But the thickness of the pelts in the collar and area of hiding yak next to the floor remains higher by about 25-40%. Yaks are known to have three main categories of hair: long hairs (in the abdominal region of the hide and on the tail), short hairs (on the rump, neck and back) and down inches (in all parts of the animal’s fur). Long coats are also the thickest. The hair length of newborn yaks is 187.60±2.66 mm, of one-year-old yaks 452.50±2.52 mm, and of six-year-old yaks, it reaches 475 mm. The authors determined the total amount of hair of different categories in the three topographic parts of the body: in the rump, the sum of hair is 1545 hair units; in the collar part, the sum of hair is 1590 hair units; in the abdominal area of the body the sum of hair is 1770 hair units. The collar and rump do not have long hairs, unlike the abdomen. Downy hairs are characteristic of all parts of the hide but predominate in the scruff and collar. The dynamics of age-related changes in the hair cover of yaks have been determined.

ImmuneDEX: updated genomic estimates of genetic parameters and breeding values for Australian Angus cattle

Context Immune competence is a proxy trait for general disease resistance and is based on combined measures of an animal’s ability to mount both a cell-mediated immune response (Cell-IR) and an antibody-mediated immune response (Ab-IR). On the basis of previously described arithmetic, we combined these measures into a single proxy trait for immune competence, named ImmuneDEX (IDEX). Aims Using a population of 3715 Australian Angus steers (n = 2395) and heifers (n = 1320) with genotypes for 45 364 single-nucleotide polymorphisms, we provide the latest genomic estimates of heritability and genetic correlations for IDEX and the components Cell-IR and Ab-IR immune competence phenotypes. Accuracy and bias of genomic predictions of breeding values are also presented and discussed. Methods Measures of Cell-IR, Ab-IR and IDEX were analysed jointly in a tri-variate genomic restricted maximum-likelihood model that contained the fixed effects of contemporary group with 80 levels, the linear covariates of age at measurement and change in skin thickness at control site, and the random polygenic (genomic estimated breeding value, GEBV) and residual effects. Following Method LR procedures, we estimate accuracy, bias and dispersion of genomic predictions using a cross-validation scheme based on five year-of-birth cohorts. Key results We report genomic restricted maximum-likelihood model estimates of heritability of 0.247 ± 0.040 for Cell-IR, 0.326 ± 0.059 for Ab-IR, 0.275 ± 0.046 for IDEX. While a small positive genetic correlation (rg) was estimated between Cell-IR and Ab-IR (rg = 0.138 ± 0.095), strongly positive estimates were obtained between IDEX and Cell-IR (rg = 0.740 ± 0.044) and between IDEX and Ab-IR (rg = 0.741 ± 0.036). Averaged across the five validation sets, the accuracy of GEBV for Cell-IR, Ab-IR and IDEX was 0.405, 0.443 and 0.411 respectively. Also, some significant bias or dispersion can be expected depending on the cohort used as the validation population. Conclusions Consistent with previous findings, immune competence phenotypes are moderately heritable and accurate GEBV can be generated to allow the selection of cattle with an improved ability to mount a general immune response. Implications Our analyses suggest that ImmuneDEX will provide a tool to underpin long-term genetic strategies aimed at improving the immune competence of Australian Angus cattle in production systems, which, in turn, is expected to reduce the incidence of disease and our reliance on antibiotics to treat disease.

The Pharmacokinetics and Skin Changes in Rabbit on Selamectin Solution

Background: It found that many antiparasitic drugs have a good treatment effect on the pets skin diseases. whether selamectin solutions have a bad effect on skin tissues needs more research. Objective: In order to investigate the effect of selamectin solution on skin changes in rabbit. Method: the skin of rabbit was given different doses of selamectin solution, then the quantity of per unit area of hair follicles and sebaceous glands and the morphology structure of them and the changes of skin thickness, epidermis thickness and dermis thickness were researched through routine paraffin sections and H. E, Evg, Masson’s, Pas, Toluidine blue staining and Image Pro Plus6.0. The concentration of blood and urine were measured by High Performance Liquid Chromatography after administration. Results: The thickness of skin, per unit of hair follicles and sebaceous glands all had an obviously increasing trend with the increasing of doses. 1 day later, the blood and urine concentration of the selamectin and the metabolite avermectin all were in highest. Many selamectin solution were excluded in the form of prototypes and many were in avermectin. There were a higher blood concentration and metabolite avermectin until 72h. Conclusion: The skin appearance changes were insignificant while the skin thickness and accessory organs increased obviously in microscope after given more selamectin. In 24h, the blood and urine concentration all were in highest. This study, which discussed the influences by selamectin solution in rabbit skin and the pharmacokinetics, was to provide the basis for the deeper study of Antiparasitic drugs effects on pet skin diseases.

Photodynamic Therapy Using HMME for Port-Wine Stains: Clinical Effectiveness and Sonographic Appearance.

This study aims at exploring the clinical efficacy and sonographic changes of photodynamic therapy (PDT) using Hematoporphyrin Monomethyl Ether (HMME) for the treatment of port-wine stains (PWS). Forty-five patients with PWS were recruited between March 2017 and June 2018 from the Department of Dermatology of The Third Affiliated Hospital of Soochow University. Five cases were of the pink type, thirty-nine cases were of the purple-red type, and one case was of the thickened type. All patients received three treatment sessions of PDT. After covering normal skin outside the treated area, patients received an intravenous injection of 5 mg/kg HMME within 20 minutes. The affected areas were exposed to a 532 nm LED light and were kept vertically at a distance of 10 cm. The irradiation energy density was set between 80 and 110 J/cm2 in 15-minute sessions. Intermittent power density adjustment was performed at a rate of 5 mW/cm2, and the treatment was withheld when the endpoint reaction appeared. Three follow-ups were performed before and after treatment, respectively, and the efficacy, thickness, and density of skin before and after treatment were evaluated with high-frequency ultrasound. The overall efficacy rate was 97.78% in forty-five cases after treatment for three sessions. Efficacy was related to age (P = 0.029) and lesion severity (P < 0.001). There were significant differences in the efficacy between the groups of <18 years old, 18-29 years old, and >29 years old (P = 0.029). A marked decrease in the numbers of distorted enlarged blood vessels per unit of the lesion was observed under high-frequency ultrasound. There were significant differences in skin thickness and skin density before and after treatment (F = 14.528, 5.428, P < 0.001). The swelling was reported to varying degrees in the treated areas in 23 patients with cheek lesion and in 6 frontal lesions. Hyperpigmentation after inflammation was observed in four patients that faded spontaneously after two months. In conclusion, photodynamic therapy for the treatment of PWS using HMME is effective and safe with few adverse reactions. Moreover, monitoring the changes in skin thickness and density of lesion tissue using high-frequency ultrasound can objectively evaluate the clinical efficacy of HMME photodynamic therapy and provide the basis for the formulation of individualized photodynamic therapy.

The Efficacy of Hyaluronidase in Early Surgery of Nasal Bone Fracture.

A nasal bone fracture is one of the most common facial injuries and is often treated by closed reduction. Typically, 2 to 3 weeks are needed for patients to return to daily life because the operation is performed after swelling around the fracture site is reduced. This study aimed to investigate that hyaluronidase injection could reduce swelling, perform early operation and return to daily life accelerated.From January 2017 to December 2017, 181 patients with nasal bone fracture were analyzed. 60 patients underwent hyaluronidase injection and massage to reduce edema, then performed surgery within 2 to 4 days. The remaining patients were treated conservatively (massage alone); they then underwent surgery. Ultrasonography was used to measure changes in skin thickness, and the treatment duration, outcome, and patient satisfaction were compared.The duration from injury to surgery was short in the early operation group, and the period of recovery and return to ordinary life was significantly shorter than in the conventional group. The difference in skin thickness after hyaluronidase injection and massage was 0.8 mm in the early operation group; there was no significant difference in the conventional group. There was no statistically significant difference in satisfaction between the 2 groups, but the mean satisfaction was higher in the early operation group.In patients with nasal bone fracture after facial trauma, hyaluronidase injection, and massage led to reduced edema. This might improve patient satisfaction by allowing earlier operation and earlier return to daily life.

Transient receptor potential ankyrin 1 (TRPA1) positively regulates imiquimod-induced, psoriasiform dermal inflammation in mice.

Transient receptor potential ankyrin 1 (TRPA1), a membrane protein ion channel, is known to mediate itch and pain in skin. The function of TRPA1, however, in psoriasiform dermatitis (PsD) is uncertain. Herein, we found that expression of TRPA1 is highly up‐regulated in human psoriatic lesional skin. To study the role of TRPA1 in PsD, we assessed Psoriasis Severity Index (PSI) scores, transepidermal water loss (TEWL), skin thickness and pathology, and examined dermal inflammatory infiltrates, Th17‐related genes and itch‐related genes in c57BL/6 as wild‐type (WT) and TRPA1 gene knockout (KO) mice following daily application of topical IMQ cream for 5 days. Compared with WT mice, clinical scores, skin thickness change and TEWL scores were similar on day 3, but were significantly decreased on day 5 in IMQ‐treated TRPA1 KO mice (vs WT mice), suggesting reduced inflammation and skin barrier defects. Additionally, the relative area of epidermal Munro's microabscesses and mRNA levels of neutrophil inducible chemokines (S100A8, S100A9 and CXCL1) were decreased in the treated skin of TRPA1 KO mice, suggesting that neutrophil recruitment was impaired in the KO mice. Furthermore, mast cells, CD31+ blood vascular cells, CD45+ leukocytes and CD3+ T cells were all reduced in the treated skin of TRPA1 KO mice. Lastly, mRNA expression levels of IL‐1β, IL‐6, IL‐23, IL‐17A, IL‐17F and IL‐22 were decreased in TRPA1 KO mice. In summary, these results suggest a key role for TRPA1 in psoriasiform inflammation and raising its potential as a target for therapeutic intervention.

Respiratory Motion Sensor Measuring Capacitance Constructed across Skin in Daily Activities.

In this work, a respiratory sensor is studied, measuring the capacitance constructed by attached electrodes on the abdomen. Based on previous findings, that skin thickness changes caused by respiration provides the signal, the fitting condition of the electrode on the skin is stabilized using a 7-μm-thick dressing film. This film can be comfortably worn for a long time, while maintaining the electrode’s position on the skin. This stabilized setup enables the detection of, not only respiration, as the cyclic capacitance change, but also of minute body volume changes over the daytime, as a change in the base line indicates the quality of the sensor signal. For this demonstration, the respiration signal is measured during the daily activity of exercise and 6-min walks.