Changes In Serum Lipoproteins Research Articles

The “Post-heparin Pattern” in Routine Serum Protein Immunoelectrophoresis

For some time following intravenous administration of heparin to non-fasting normal subjects, routine immunoelectrophoretic preparations show characteristic changes in serum lipoproteins. These alterations are of two types: (1) marked anodal mobility increase, the alpha-2 (beta) component locating in the alpha-1 region while the alpha-1 appears as a pre-albumin; (2) conformational alterations of the immunoprecipitin arcs comprising elongation of the alpha-2 (beta) arc, and splitting of the alpha-1 arc into two distinct entities. Familiarity with these patterns should minimize the possibility of interpretative errors with specimens from patients receiving heparin.

Changes in serum lipoproteins during metamorphosis of the bullfrog, Rana catesbeiana

Serum lipoproteins of the bullfrog, Rana catesbeiana, were studied during metamorphosis. Adult bullfrog has essentially one lipoprotein, designated β-lipoprotein. This β-lipoprotein migrates during electrophoresis to β-globulin region and it has a low hydrated density such that it exhibits floatation in a solvent of density 1.063. On the other hand, tadpole serum has one more lipoprotein, designated as α-lipoprotein, in addition to the β-lipoprotein. The α-lipoprotein migrates to the α-globulin region in zone electrophoresis and corresponds to the so called high density lipoprotein judging from ultracentrifugal behavior. Serum α-lipoprotein disappears and β-lipoprotein content decreases during metamorphosis.

Rapid serum lipoprotein changes in spider monkeys on short-term feeding of high cholesterol-high saturated fat diet.

SummarySerum lipoprotein changes resulting from high cholesterol (0.5% and 2.0%)-high saturated fat diets have been studied in spider monkeys (Ateles geoffroyi). Rapid increase in β-lipoprotein, significant elevation in α-lipoprotein, and marked variations in individual responses to cholesterol intake were observed during these dietary periods.

Changes in serum lipoproteins after parenteral feeding with emulsified fats

Changes in serum lipoproteins after parenteral feeding with emulsified fats

Physical-chemical changes in serum lipoproteins during incubation of human serum

Incubation of human serum for 24 h at 37° resulted in a marked shift in the ultracentrifugal distribution of the high-density lipoprotein (HDL) fraction. The serum concentrations of the F° 1.20 0–3.5 (HDL 3) decreased while those of the F° 1.20 3.5–9.0 (HDL 2) increased. Inhibition of serum fatty acid transferase activity by p-hydroxymercuribenzoate did not inhibit the incubation-induced change in HDL distribution. A significant reduction in the protein content of the HDL, isolated from incubated serum, was detected. These data suggest that the shift in HDL distribution may result from an initial dissociation of protein from HDL 3 followed by an association of relatively lipid-rich lipoprotein residues to give HDL 2-type lipoproteins. Small order increases in S° f rates of the major peak of the S° f 0–20 fraction were also observed, during incubation of serum, in the presence as well as in the absence of transferase activity. The magnitude of the increase in s f 5 rate was found to be directly related to the level of S° f 20–400 lipoproteins in the serum and to be greater in the presence of transferase activity.

Changes in serum lipoproteins after a large fat meal in normal individuals and in patients with ischemic heart disease

A fat-tolerance test has been used to measure alterations in lipid transport in normal individuals and in patients with ischemic heart disease. Serum lipoproteins and cholesterol were measured after the ingestion of 120 Gm. of butterfat. Greater elevations of the triglyceride containing Sf 20–100 and Sf 100–400 fractions were noted in patients with heart disease and in normal hypercholesterolemic individuals. The implications of these differences are discussed with relation to the presence of a basic metabolic difference in these individuals

Changes in serum lipo-proteins in psoriasis.

If psoriasis is a disease of systemic lipid metabolism, some deviation from the normal should be found in the serum lipoprotein levels of psoriatic patients. It has been shown that the lipid constituents in the serum, including phospholipids, neutral fats, triglycerides, cholesterol, and cholesterol esters, are carried primarily as lipid-protein complexes called lipoproteins. 1,2 The best method at present for studying these relatively unstable compounds is by the technique of ultracentrifugation. This study was undertaken to determine what changes, if any, occur in serum lipoprotein fractions of patients with psoriasis when treated by various means. In recent publications it has been demonstrated that patients with psoriasis show no consistent variation from the normal with regard to serum lipoproteins. No significant abnormalities were found by Lea et al. 3 in the serum lipoproteins of patients with psoriasis, and these findings were confirmed in our own preliminary report. 4 The possibility remains,

The effect of 1-thyroxine on serum lipoprotein and cholesterol concentrations.

ABSTRACT The effect of intravenous administration of 6 mg. per week of l-thyroxine for a period of sixteen weeks on low-density and high-density serum lipoproteins, total serum cholesterol, body weight, pulse and blood pressure was studied in a group of 13 institutionalized ambulatory schizophrenic patients. S°f0–12 and S°f12–20 serum lipoprotein concentrations were significantly reduced, but no effect on S°f20–100 and S°f100–400 was observed. Total serum cholesterol as well as high-density serum lipoprotein concentrations were also significantly reduced. The reuction in low-density serum lipoprotein concentrations was found to be highly correlated with initial (pre-treatment) levels, resulting in greater decreases in serum lipoprotein concentrations the higher the initial level. Body weight and diastolic blood pressure decreased significantly and pulse rate increased. l-Thyroxine is an effective hypolipoproteinemic substance, producing a pattern of changes in serum lipoprotein and total serum cholesterol...

Serum Lipoprotein Changes During Fasting in Man

Serum Lipoprotein Changes During Fasting in Man

Serum lipoprotein changes during fasting in rabbits.

Serum lipoprotein changes during fasting in rabbits.