Appropriate nutrition is important for both health and optimization of sport performance; however, many athletes do not have sufficient nutrition knowledge to engage in ideal nutrition practices. Few studies have been conducted to investigate the efficacy of a nutrition education intervention on athletes’ nutrition knowledge, and even fewer have done so using a validated assessment tool. PURPOSE: To evaluate changes in nutrition knowledge of NCAA Division I women’s basketball players after a sport-focused nutrition education intervention using a validated nutrition knowledge survey. METHODS: NCAA Division I women basketball players (N=8) completed a validated nutrition knowledge survey (Calella et al., 2017) that evaluated both general and sport nutrition. Athletes then participated in six, once weekly, 20-minute nutrition education sessions led by a Registered Dietitian. Topics included: hydration, carbohydrates, protein, fats, meal planning, and micronutrients/supplements. At the end of six weeks, the survey was re-administered. Scores were summed as +1 for a correct answer and +0 for no answer, an incorrect answer, a double-answer, or the “I don’t know” option. Maximum possible score was 97. A paired-samples t-test was conducted to evaluate baseline versus follow-up scores. RESULTS: At baseline, athletes scored 40.5±18.0 out of 97 possible, 27.0±12.7 out of 64 possible, and 13.9±6.2 out of 33 possible for the total survey, general nutrition and sport nutrition sections, respectively. Significant average score increases were found for the total survey overall (62.2±11.7, p=0.004), within the general nutrition section (40.4±7.7, p=0.015), and within the sport nutrition section (21.9±5.9, p<0.001). CONCLUSION: The education intervention used in this pilot study succeeded in increasing nutrition knowledge of Division I women’s basketball players. Future research should include intake assessments to determine whether eating habits improve after nutrition education. This research was funded by the MSU College of Education via a Summer Research Fellowship.