When the arch rib of the mid-bearing through and lower-bearing through arch bridges undergoes out-of-plane deformation, it is usually subject to the resilience force provided by the flexible hanger, which is known as the “non-conservative force effect” of the suspender. In contrast to the static condition, in the dynamic scenario, the time-varying non-conservative force exerted by the flexible suspender becomes more complex due to dynamic changes in external load. Moreover, the difference in fundamental frequency and vibration period between the bridge system and arch rib may influence the stress distribution within the arch rib during ground motion. This paper investigates the impact of time-varying non-conservative forces on the dynamic stability of arch ribs in concrete-filled steel tube (CFST) bridges under seismic loads. Specifically, it examines the influence of different seismic waveforms, frequency disparities between bridge slabs and arch ribs, and suspender stiffness on the non-conservative effect. The results reveal significant disparities in the impact of non-conservative forces exerted by the suspender during seismic events with identical intensity but varying frequency characteristics. The influence of non-conservative forces on the dynamic stability of bridges escalates as deck stiffness increases, while it remains relatively unaffected by changes in suspender stiffness.