Background and objectives: Land use and land cover change (LULCC) directly affects the human living environment and economic society by influencing ecosystem services. This study explores the complex relationship between LULCC and ecosystem services in the Dongting Lake region of China.Methods: The analysis incorporates the transfer matrix analysis of LULCC, ecosystem service value (ESV) accounting, Mann–Kendall mutation test, gray correlation, and path analysis. This study explored the spatial and temporal characteristics of LULCC and ecosystem services and revealed the comprehensive impacts of LULCC on ecosystem services from the perspective of factor interactions.Findings: The findings reveal that open water (OW) area and farmable land are the dominant land use types transferring in and out of the study area, with significant interconversion observed among open water area, wetland (WL), arable land (AL), and forest land (FL). Water conservation, waste disposal, climate regulation, and biodiversity conservation emerge as the most crucial ecosystem services in the study area. The study identifies consistent patterns of abrupt changes in LULCC and ESV across six analyzed time periods. Forest land exhibits the most substantial direct influence on climate regulation, gas exchange, soil formation and protection, conservation of biodiversity, raw materials, and recreation and leisure. Additionally, forest land, wetland, arable land, and open water area contribute significantly to the total ESV, with forest land being the dominant factor influencing the spatial differentiation of ecosystem services in the Dongting Lake region.Conclusion: The research reveals the consistency of ecosystem services and LULCC trends in the Dongting Lake area. LULCC is a complex process influenced by multiple factors, in which the conversion between ecological and non-ecological land use has a significant impact, and the complex coupling and coordination among LULCC drivers together influence ecosystem services. Furthermore, LULCC alters the structure and scale of ecosystems, resulting in alterations in the ESV.