Defects have a significant effect on the dynamic fracture characteristics of the medium. In this paper, the dynamic fracture experiment of specimens with bias precracks is designed by utilizing the digital laser dynamic caustics system, and the effect of defect eccentricity on the dynamic fracture behavior is studied. Research shows that crack propagation can be divided into four stages: crack initiation stage, attraction stage, repulsion stage, and specimen fracture stage. The change of defect eccentricity has no obvious effect on the crack propagation behavior in the crack initiation stage and penetration stage but has a significant effect on the attraction stage and specimen fracture stage. In the process of the interaction between defect and crack, mode I stress intensity factor decreases at first and then increases. The decrease of mode I stress intensity factor reduces with the increase of defect eccentricity. The value of mode II stress intensity factor changes from negative to positive. With the increase of defect eccentricity, the symbol of mode II stress intensity factor no longer changes. The fractal dimension and the deflection angle of crack trajectory both decrease with the increase of defect eccentricity. In addition, a numerical simulation of the experiment is conducted by ABAQUS, which provides results that are in good agreement with the experimental results.