Rheological Changes Research Articles

The impact of calcined mud on the rheological properties of eco-friendly concretes using the equivalent mortar method (CEM)

In order to harmonise technical and environmental aspects, people today focus not only on concrete's technical characteristics but also on environmental imperatives. Thus, the emphasis is on the development of concrete and cements that offer ecological and sustainable advantages. Commonly, people use various natural materials as cement additives, demonstrating their effectiveness by replacing a significant portion of the cement in the hardened state of concrete. This substitution leads to a substantial and remarkable reduction in carbon dioxide emissions. However, when it comes to the physical properties of the material in its fresh state, challenges may arise. Indeed, the rheological changes induced by these additives may hinder the on-site use of this eco-concrete. The main purpose of this study is to find out how adding minerals to environmentally friendly concrete changes its rheological parameters (yield stress, plastic viscosity) and fresh properties (slump, flow time). We will approach this using the concept of mortar equivalent to concrete (CEM). We developed five formulations of CEM’s by substituting cement with calcined mud (CM) in binary mixing systems at a temperature of 20 °C. The results showed that adding binary cements with calcined mud had a negative effect on the fresh properties and rheological parameters. However, when substitution happens at a low rate, it behaves almost like control mortar (OPC).

Irregular Eccentric Wellbore Cementing: An Equivalent Circulation Density Calculation and Influencing Factors Analysis

In the field of cement, if the formation cannot be given sufficient pressure to maintain stability during construction, pressure control failure may occur, leading to the leakage of liquids and gasses from the formation to the wellbore. In addition, irregular wellbore diameter and casing eccentricity are important factors that are easily overlooked and affect the prediction of ECD (Equivalent Circulation Density) calculation. This results in major accidents and ecological disasters, further impacting the global environment. This study focuses on a well in the eastern oilfields of China, and based on a rheological experiment of high temperature and high pressure, an irregular eccentric wellbore model is established according to the measured wellbore diameter and eccentricity data to calculate the ECD of the whole cementing process. Then, a data set is constructed and analyzed using the random forest method to quantitatively evaluate influencing factors such as displacement, rheology, density, and eccentricity on the bottomhole and wellbore ECD. Results find that the density of cement slurry and drilling fluid has the most significant impact on the maximum ECD, with the impact reaching 0.3142 and 0.2902, respectively, and the main factors that affect the minimum ECD are the density and rheological changes in the drilling fluid, reaching 0.7014 and 0.2846. These research findings will contribute to the precise control of wellbore pressure during cementing operations, further ensuring the safety of cementing operations, and laying a technical foundation for the automation and intelligentization of subsequent cementing operations.

Using Rheology and Image Processing to Study the Effects of Cellulose Nanocrystal Sedimentation.

The effect of sedimentation on lyotropic liquid crystalline dispersions is both an interesting subject in colloidal science and is of practical importance for understanding changes that can occur during dispersion storage. This research explored how the seemingly subtle changes in average length resulting from a single sedimentation step affected the rheological properties and self-assembly of aqueous dispersions of sulfated cellulose nanocrystals. Sedimentation of a primarily isotropic aqueous cellulose nanocrystal dispersion for 1 month at ambient conditions resulted in an isotropic top phase and a biphasic bottom phase, which were separated for further study. Both atomic force microscopy measurements and intrinsic viscosities determined via Fedor's equation showed preferential fractionation of longer cellulose nanocrystals (CNCs) to the bottom phase. The effects of this fractionation on dispersion rheology and phase behavior were studied by preparing a range of concentrations from each phase. As expected, the concentration for the isotropic-biphasic phase transition was significantly lower for the bottom phase than for the top phase or parent dispersion. Rheological changes were generally subtle, but significant differences were seen in the storage modulus at concentrations approaching rheological gelation. Most notably, quantitative image processing showed that even this simple, single-step fractionation process had a significant impact on the relative proportion of tactoids and chiral helices with planar and homeotropic anchoring in self-assembled cellulose nanocrystal films. These results highlight the impact that changes in polydispersity due to sedimentation can have on the self-assembly of nanomaterial mesogens.

Influence of chemical admixtures on buildability and deformation of concrete for additive manufacturing

In this study, the buildability of three-dimensionally (3D) printed (3DP) concrete that uses coarse aggregate was evaluated in the fresh state according to the contents of three chemical admixtures (high-range water-reducing agent (HRWRA), viscosity-modifying agent (VMA), and setting-time modifying agent), and the correlation between fluidity changes and buildability was analyzed. Polycarboxylate HRWRA and VMA contents were varied to control the flowability and static yield stress, which affect the workability. In addition, the SMA content was varied, and slump tests and table flow tests were performed at 30-minute intervals up to 6.5 h after mixing to measure flowability. Simultaneously, static yield stress was measured to analyze rheological changes, and penetration resistance and green strength tests were performed to analyze the main factors influencing buildability over time. The highest buildability was observed when the VMA content was 1.5 %. The static yield stress of fresh concrete could be ≤3000 Pa to guarantee adequate 3DP concrete transport performance while that at 1 h after mixing could be ≥2000 Pa to secure buildability. When the penetration resistance of fresh concrete is ≥80 kPa, the deflection of concrete may be <10 %. Through this, buildability can be approximately judged based on penetration resistance.

Could oxygen gradient ektacytometry help to detect sickle cell trait carriers at risk for kidney disorders or exercise-related complications?

The study of Ellsworth etal. (Br J Haematol, 2024) demonstrated the usefulness of oxygen gradient ektacytometry technique to better identify the physiological parameters that could increase the risk of sickling of red blood cells (RBCs) from sickle cell trait (SCT) carriers. Oxygen gradient ektacytometry combined with pH and osmolality modulations could help in identifying SCT carriers at risk for kidney disorders or exercise-related complications. Other factors than the percentages of haemoglobin S are probably involved in the propensity of RBCs from SCT carriers to sickle during deoxygenation. Commentary on: Ellsworth etal. Hypertonicity and/or acidosis induce marked rheological changes under hypoxic conditions in sickle trait red blood cells. Br J Haematol 2024; 205:1565-1569.

Hypertonicity and/or acidosis induce marked rheological changes under hypoxic conditions in sickle trait red blood cells.

Deformability and sickling of red blood cells (RBCs) from individuals with sickle cell trait (SCT) was evaluated under harsh biophysical conditions that mimic certain vascular beds invivo. RBC deformability in osmotic-gradient ektacytometry was decreased in HbAS (SCT) compared to HbAA (wild-type) RBCs at supraphysiological osmolalities. RBC deformability was also measured by oxygen-gradient ektacytometry. Whereas RBC sickling was not observed under isotonic and neutral pH conditions, hypertonicity and acidosis alone or in combination induced reversible sickling of SCT RBC. These data suggest that hyperosmolality and/or acidosis enhance hypoxia-induced sickling of SCT RBC.

Interrogating an along-strike variation in the evolution and rheology of a large continental strike-slip fault zone

Along-strike variations in lithology, temperature, fluid activity, etc. can induce rheological changes in strike-slip faults that may be recorded by different fault rocks and fabrics. This article interrogates localized formation of granite-derived mylonite in a large strike-slip fault zone in which ultramylonite is the principle granite-derived fault rock to better understand this rock record of faulting. Microtextures show that rate-limiting deformation mechanisms in mylonite were dislocation creep in quartz and diffusion creep in very-fine-grained feldspar aggregates. Microtextures also show that mylonite formation is fully compatible with continuous viscous deformation whereas coeval ultramylonite along strike formed after whole-rock cataclasis at the brittle-viscous transition. Differential stresses determined from quartz aggregates in ultramylonites are 40–150% greater than stresses in mylonites. Hence, mylonites represent a local weak sector within this otherwise relatively strong fault zone. Mylonite formation is correlated with syndeformational chemical alteration as well as quartz microstructures and mineral assemblages indicating elevated deformation temperatures. Not all mylonite samples record significant chemical alteration. Therefore, mylonite formation likely records locally elevated temperatures. These results illustrate how a local shift in deformation conditions can affect the evolution and rheology of a large strike-slip fault zone, and how fault rocks record these processes.

Dilute Polymer Droplets Show Generalized Wetting Dynamics via an Average Viscosity.

Despite the prevalence of non-Newtonian fluids in various practical applications, comprehensive dynamic wetting models are lacking. Existing models often oversimplify complex rheological behavior, limiting our ability to predict wetting dynamics. This work introduces and experimentally validates a generalized model for the dynamic wetting of non-Newtonian shear-thinning fluids (dilute polymer solutions) on solid substrates. We experimentally analyzed 12 different shear-thinning fluids using both a power-law model and Carreau-Yasuda model. The data clearly show that the dynamic contact angle can be generalized using an average viscosity to capture rheological changes during droplet spreading. The average viscosity was defined using the fluid's constitutive model over shear rates relevant to the spreading process. Using a small droplet approximation, we propose and validate a semianalytical spreading model to predict the basal radius of non-Newtonian droplets. The model agrees well with the experimental data. Additionally, the average viscosity was used to define a spreading time scale, which is capable of collapsing the spreading of different non-Newtonian fluids onto a master spreading curve. This work offers significant potential for predicting the dynamic shape and spreading of non-Newtonian fluids with complex rheologies in a range of applications and industrial processes.

Self-assembly of mixed-linkage glucan hydrogels formed following EG16 digestion

Mixed-linkage glucans are major components of grassy cell-walls and cereal endosperm. Recently identified plant endo-β-glucanase from the EG16 family cleaves MLGs with strong specificity towards regions with at least four sequential β(1,4)-linked glucose residues. This activity yields a low molecular-weight MLG with a repeating structure of β(1,3)-linked cellotriose that gels rapidly at concentrations as low as 1.0 % w/v. To understand the gelation mechanism, we investigated the structure and behavior using rheology, microscopy, X-ray scattering, and molecular dynamics simulations. Upon digestion, the material's rheological behavior changes from typical polymeric material to a fibrillar network behavior seen for e.g. cellulose nanofibrils. Scanning electron microscopy and confocal microscopy verifies these changes in micro- and nanostructure. Small-angle X-ray scattering shows in-solution self-assembly of MLG through ~10 nm elemental structures. Wide-angle X-ray scattering data indicate that the polymer association is similar to cellulose II, with dominant scattering at d-spacing of 0.43 nm. Simulations of two interacting glucan chains show that β(1,3)-linkages prevent the formation of tight helices that form between β(1,4)-linked d-glucan chains, leading to weaker interactions and less ordered inter-chain assembly. Overall, these data indicate that digestion drives gelation not by enhancement of interactions driving self-assembly, but by elimination of unproductive interactions hindering self-assembly.

Antimicrobial adhesive self-healing hydrogels for efficient dental biofilm removal from periodontal tissue

ObjectivesOral biofilms, including pathogens such as Porphyromonas gingivalis, are involved in the initiation and progression of various periodontal diseases. However, the treatment of these diseases is hindered by the limited efficacy of many antimicrobial materials in removing biofilms under the harsh conditions of the oral cavity. Our objective is to develop a gel-type antimicrobial agent with optimal physicochemical properties, strong tissue adhesion, prolonged antimicrobial activity, and biocompatibility to serve as an adjunctive treatment for periodontal diseases. MethodsPhenylboronic acid-conjugated alginate (Alg–PBA) was synthesized using a carbodiimide coupling agent. Alg–PBA was then combined with tannic acid (TA) to create an Alg–PBA/TA hydrogel. The composition of the hydrogel was optimized to enhance its mechanical strength and tissue adhesiveness. Additionally, the hydrogel’s self-healing ability, erosion and release profile, biocompatibility, and antimicrobial activity against P. gingivalis were thoroughly characterized. ResultsThe Alg–PBA/TA hydrogels, with a final concentration of 5 wt% TA, exhibited both mechanical properties comparable to conventional Minocycline gel and strong tissue adhesiveness. In contrast, the Minocycline gel demonstrated negligible tissue adhesion. The Alg–PBA/TA hydrogel also retained its rheological properties under repeated 5 kPa stress owing to its self-healing capability, whereas the Minocycline gel showed irreversible changes in rheology after just one stress cycle. Additionally, Alg–PBA/TA hydrogels displayed a sustained erosion and TA release profile with minimal impact on the surrounding pH. Additionally, the hydrogels exhibited potent antimicrobial activity against P. gingivalis, effectively eliminating its biofilm without compromising the viability of MG-63 cells. SignificanceThe Alg–PBA/TA hydrogel demonstrates an optimal combination of mechanical strength, self-healing ability, tissue adhesiveness, excellent biocompatibility, and sustained antimicrobial activity against P. gingivalis. These attributes make it superior to conventional Minocycline gel. Thus, the Alg–PBA/TA hydrogel is a promising antiseptic candidate for adjunctive treatment of various periodontal diseases.

Fire resistance of phosphoric acid activated metakaolin geopolymer

Fire resistance is a key advantage of geopolymer (GP) materials. While most research focuses on alkali-activated GPs, this study examines the fire resistance of phosphoric acid-activated GPs with varying phosphorus-to-aluminum (P/Al) molar ratios. By activating metakaolin with different ortho-phosphoric acid (H3PO4) solutions, it was found that GPs with P/Al > 0.59 exhibited intumescence during fire tests, resulting in a 110 °C lower backside temperature (310 °C) compared to non-intumescent GPs (420 °C). Physico-chemical changes were analyzed before and after fire tests using DMA, TGA NMR, EPMA, and XRD. The study revealed dehydration, crystallization, and rheological changes, with NMR showing transitions from Al(VI) to Al(IV), full condensation of phosphorus species, and Si-O-Al to Si-O-Si bonds. Intumescence was linked to GP softening between 100 and 160 °C, achievable only with a P/Al ratio ≥ 0.74, with no additional benefits from excess phosphorus.

Lawsonite eclogite records subduction rheology and slab detachment, Faro, Yukon-Tanana Terrane, Yukon Territory, Canada

Lawsonite eclogites represent cold subduction in the rock record, and new localities provide rare natural field laboratories to investigate deep deformation processes. We present a new lawsonite eclogite locality near Faro, Yukon Territory, Canada, the first reported lawsonite eclogite locality of the Yukon-Tanana Terrane. The discovery indicates that mid- to Late Permian subduction in the Yukon-Tanana Terrane subducted cold material in an oceanic subduction zone, in contrast to most previous models. We define a pressure-temperature-deformation (P-T-d) pathway for the lawsonite eclogites using a combination of microstructures, mineral compositional mapping, crystal orientation mapping, and phase equilibrium modeling. Metamorphism in the Faro eclogites followed a clockwise pathway through the lawsonite eclogite facies, reaching peak pressures of 22−24 MPa at 525−550 °C. Rocks then exhumed to 14.5−16 MPa during heating to 630−655 °C. Crystallographic preferred orientations (CPOs) in omphacite developed dominantly by dissolution-precipitation creep and oriented grain growth concurrently with CPOs developed in glaucophane by a combination of dislocation- and dissolution-precipitation creep with oriented grain growth. We link this fabric and CPO development in omphacite and amphibole to lawsonite dehydration, slab decoupling, and initiation of exhumation of the eclogites, providing evidence that lawsonite dehydration plays a significant role in changes in subduction zone rheology.

Rheological behavior during thermoplastic deformation of metallic glasses: A molecular dynamic simulation

Nanoimprint is a low-cost and effective method to manufacture nanostructures for metallic glasses with a precision replication of the mould shape and control of size. However, the atomic structure of the materials changes during the processing, which affects the practical application and the performance of the nanostructures. This crucial issue has not been addressed as thoroughly as it deserves. Here, we investigate the rheological behaviors of metallic glasses under the deformation with different temperatures and strain rates via molecular dynamic simulation. We find an abnormal rheological mode change in the non-Newtonian region, which is attributed to the evolution of short- and medium-range ordered structures. Mechanical deformation leads to the destruction and regeneration of atomic short-range ordered structures at all strain rate ranges. Their total amount and distribution remain at a similar level when the strain rate is low. When the strain rate exceeds a critical value, the deformation accelerates the relaxation by shortening the β-relaxation, resulting in the decrease of the total amount of short-range ordered structure and reorganization in the medium-range ordered structure. Furthermore, the results show a significant inheritability from the sample during deformation to the cooled-down sample, demonstrating the influence of deformation history on the properties of materials manufactured with the nanoimprint. The deformation during the nanoimprinting could reduce the ultimate strength and increase the plasticity of the materials, which provides a potential method to precisely turn the properties of metallic glasses by controlling the rheological process in the nanoimprint possessing.

Use of optical absorption and scattering properties to monitor the change of chemical characteristics, particle structure and viscoelasticity during apple puree processing

This work explored the optical absorption (μa) and reduced scattering (μs') properties at 400–1050 nm (Vis-NIR) and 900–1650 nm (SWIR) of apple puree at different heating times to evaluate their changes in composition and structure. The optical absorption and scattering coefficients of μa and μs' decreased with increasing heating time. The specific μs' coefficients at 432 nm, 455 nm, 1183 nm and 1304 nm were related to particle structural changes. The partial least squares regression (PLSR) models based on μs' coefficients in the combined Vis-NIR and SWIR regions provided satisfactory estimations of puree particle area (Rp2 = 0.90) and particle perimeter (Rp2 = 0.85), whereas the μs' coefficients in the Vis-NIR region predicted puree viscosity (Rp2 = 0.83), elasticity modulus (Rp2 = 0.81) and viscous modulus (Rp2 = 0.78). Consequently, optical properties can be a potential tool to determine structural and rheological changes of apple puree.

Rheological Changes in Bio-Based Filaments Induced by Extrusion-Based 3D Printing Process.

In this work, the authors investigated the impact of extrusion-based printing process on the structural characteristics of bio-based resins through rheological measurements. Two commercially available filaments made from unfilled and wood-filled polylactide (PLA) polymers were considered. Three-dimensional specimens were prepared by printing these filaments under various operating conditions, i.e., changing the extruder temperature and printing rate, and examined using time sweep tests. Specific cycle rheological testing was conducted on pelletized filaments to simulate temperature changes in the printing process. The rheological characteristics of unprocessed materials, in terms of storage (G') and loss (G″) moduli, were found to be slightly affected by temperature changes. For a pure polymer, the G' slope at a low frequency decreased over time, showing that the polymer chains evolved from a higher to a lower molecular weight. For wood-filled materials, the G' slope rose over the testing time, emphasizing the formation of a percolated network of structured filler within the matrix. On the other side, the rheological parameters of both materials were strongly impacted by the printing extrusion and the related conditions. At lower nozzle temperatures (200 °C), by decreasing the printing speed, the G' and G″ curves became increasingly different with respect to unprocessed resin; whereas at higher nozzle temperatures (220 °C), the influence of the printing speed was insignificant, and all curves (albeit distant from those of unprocessed matrix) mainly overlapped. Considerations on degradation kinetics of both materials during the printing process were also provided by fitting experimental data of complex viscosity with linear correlation over time.

A food for elders with dysphagia: Effect of sodium alginate with different viscosities on the gel properties of antarctic krill composite shrimp surimi

This study investigated the effects of sodium alginate (SA) with different viscosities on the physicochemical properties and structure of composite shrimp surimi gels made from Antarctic krill and Litopenaeus vannamei. Changes in texture, rheology, water status, thermal stability, chemical bonding, zeta potential, and microstructures of the gels were analyzed to explore their feasibility as food products for individuals with dysphagia. The addition of SA addition increased the chewiness, absolute value of zeta potential, WHC, and thermal stability of the composite shrimp surimi gels. Concurrently, it decreased the gel strength and the content of chemical bonds, such as ionic and hydrogen bonds, with these changes being more significant at higher SA viscosities. The water distribution analysis showed that increasing SA viscosity increased the content of immobilized water within the gels and stabilized the water. From the perspective of zeta potential and microstructure, higher SA viscosities significantly increased the absolute value of zeta potential, enhanced the electrostatic repulsion and spatial potential resistance, and resulted in larger microstructural gaps, which improved the moisture-trapping capacity and WHC of the gels. The microstructure was more homogeneous and continuous at an SA viscosity of 800 mPa s. International Dysphagia Dietary Standardization Initiative (IDDIS) testing showed that gels with added SA can be classified into Tier 6 dysphagia diets. These findings provide insights for developing nutritious dysphagia-suitable foods for elders.

Rheological properties of thermally treated and digested sludge: Implications for energy requirements of pumps and agitators

Understanding sludge rheology and optimizing equipment performance is crucial for energy efficiency in wastewater treatment plants (WWTPs). This study examined sludge rheology after thermal hydrolysis pre-treatment (THP) at 60, 80, and 120 °C for 2 h, followed by anaerobic digestion (AD) at 37 °C for 20 days, and assessed impacts on pump and agitator performance. Post-treatment, sludge showed reduced viscosity and improved flowability, indicated by changes in Herschel-Bulkley parameters, enhancing pump and agitator efficiency, particularly at 120 °C. These rheological improvements were correlated to the solubilization of sludge components after THP and solids reduction after AD, highlighting the interconnectedness of rheology and treatment outcomes. Despite high heat demands, an energy balance showed that THP scenarios, especially at 120 °C, had lower energy requirements for pumps and agitators, leading to energy savings without increased heat consumption. These findings underscore the influence of rheological changes in improving energy efficiency in WWTPs.

Entanglements of Macromolecules and Their Influence on Rheological and Mechanical Properties of Polymers.

Flexible macromolecules easily become entangled with neighboring macromolecules. The resulting network determines many polymer properties, including rheological and mechanical properties. Therefore, a number of experimental and modeling studies were performed to describe the relationship between the degree of entanglement of macromolecules and polymer properties. The introduction presents general information about the entanglements of macromolecule chains, collected on the basis of studies of equilibrium entangled polymers. It is also shown how the density of entanglements can be reduced. The second chapter presents experiments and models leading to the description of the movement of a single macromolecule. The next part of the text discusses how the rheological properties change after partial disentangling of the polymer. The results on the influence of the degree of chain entanglement on mechanical properties are presented.

Morphology, viscoelastic properties and the mechanical performance of highly toughened polyamide 6/acrylonitrile–butadiene–styrene/polylactic acid ternary blends

AbstractIn this research, the morphology, rheology, and mechanical performance of polyamide 6/polylactic acid/acrylonitrile–butadiene–styrene (PA6/ABS/PLA) ternary blends were studied. The optimum ABS percentage in PA6/ABS binary blend to achieve highly toughened blend was determined around 35 wt.%. The spreading coefficient (Sij) predictions showed that each of PLA and ABS can form separate phase in the PA6 matrix. The evaluation of the interfacial interactions in the ternary blends revealed a higher tendency of PLA to interact with PA6 chains (Sij:−1.20) rather than ABS (Sij:−0.20) which is in comply with higher work of adhesion of PLA/PA6 than PLA/ABS and PA6/ABS. By adding PLA to the binary blends of PA6/ABS, it was found that the ABS domain size decreased due to rheological change. The localization of each component in the ternary blends observed in SEM analysis was in agreement with spreading coefficient predictions. Morphological evaluations revealed higher interfacial interactions between PA6 and PLA in the blends with up to 20 wt.% PLA. The optimized blend ratio of ternary blends showed higher complex viscosity at low shear rates and higher values than the mixing rule prediction. The optimum flexural modulus of 2 GPa and impact resistance of 47 kJ/m2 were achieved at 20 wt.% PLA in PA6/ABS/PLA ternary blend.Highlights The optimum PA6/ABS blend ratio to achieve high toughness was determined. The phase localization in the blends predicted via Spreading Coefficient. The interaction of PA6 and ABS enhanced by the addition of PLA in the blends. The mechanical properties of blends are correlated with rheological behavior. The flexural modulus of blends was higher than Halpin–Tsai model predictions.

Establishment and evaluation of a premenstrual syndrome rat model induced by progesterone-withdrawal and emotional-stimulation

Premenstrual syndrome(PMS) lacks a highly consistent and feasible animal model that aligns with diagnostic and therapeutic standards in both traditional Chinese medicine(TCM) and western medicine, resulting in a lack of reliable experimental carriers for studying its pathogenesis and pharmacological effects. This study aims to systematically analyze the biological implications of PMS from the perspective of the &quot;disease-syndrome-symptom&quot; correlation and establish preparation and evaluation methods for an improved animal model of this disease. Firstly, clinical symptom gene sets related to the Qi stagnation syndromes due to liver depression and blood stasis in PMS in both modern medicine and TCM diagnostic standards were collected through GeneCards, DisGeNET, Mala-Cards, and the System of Foundational Diagnostic Association(SoFDA) database, as well as published literature. Based on the interaction information between genes, a &quot;disease-syndrome-symptom&quot; correlation network of PMS was established. Based on data mining results, an improved rat model of PMS was prepared by combining chronic restraint stress with the classical progesterone-withdrawal mo-del to simulate emotional depression caused by external environmental stimuli during the clinical onset process, inducing pathological damage from both physiological and emotional dimensions. The evaluation of the improved model before and after modification included open field experiment scores, organ indices, ovarian pathological changes, serum levels of estradiol(E_2), follicle-stimulating hormone/luteinizing hormone(FSH/LH), 5-hydroxytryptamine(5-HT), dopamine(DA), norepinephrine(NE), as well as coagulation parameters and hemorheology indexes. By calculating the degree, betweenness, and closeness centrality of nodes in the &quot;disease-syndrome-symptom&quot; correlation network, 163 core genes with topological importance were identified. Further biological function mining results indicated that core genes in PMS mainly participated in the regulation of the &quot;nervous-endocrine-immune&quot; system and pathways related to circulatory disorders. Mapping analysis of clinical phenotype symptom gene sets suggested significant correlations between core genes in PMS and depressive symptoms and pain symptoms caused by blood stasis. Compared with the simple progesterone withdrawal model, rats subjected to combined injections and restraint stress showed more significant abnormalities in open field experiment scores, ovarian tissue pathology, serum neurotransmitter levels of 5-HT and DA, as well as serum hormone levels of E_2 and FSH/LH. The modified modeling conditions exacerbated the pathological changes in blood rheology, coagulation function, and red blood cell morphology in model rats, confirming that the improved rat model could characterize the &quot;nervous-endocrine-immune&quot; system disorder and circulatory system disorders in the occurrence and progression of PMS, consistent with the clinical diagnostic and therapeutic standards of both TCM and western medicine. The establishment of the improved rat model of PMS can provide a reliable experimental carrier for elucidating the pathogenesis of PMS and discovering and evaluating therapeutic drugs. It also provides references for objectively reflecting the clinical characteristics of PMS in TCM and western medicine and precision treatment.