Changes In Modulus Research Articles

A molecular dynamics study on the size effects of Fe3O4 nanoparticles on the mechanical characteristics of polypyrrole/Fe3O4 nanocomposite

ABSTRACT Nanoparticle size effects on elastic properties of polymeric bio-nanocomposites were studied using atomistic molecular dynamics (MD) simulations. Spherical magnetite nanoparticles (MNPs) were placed at the centre of amorphous polypyrrole (PPy) polymer in three models with different sizes. Three models of nanocomposites with the same particle volume fractions with different sizes were considered. The Lennard-Jones 6-12 potential modelled the interface interaction between polymer and particles. Initially, the obtained mechanical properties for pristine PPy and magnetite in the bulk state showed an acceptable agreement with experimental data. Subsequently, simulation results illustrated that incorporating MNP into the PPy matrix improves the elastic modulus of the matrix by 28-58%, and more importantly, decreasing the size of the nanoparticle from 2.40 to 1.80 nm in the system leads to increasing Young's modulus of the nanocomposite from a 3.20 to 3.94 GPa. Furthermore, the atomic investigation demonstrated that this change in elastic modulus is due to the change in interatomic interaction between nanoparticle and polymer when the nanoparticle size changes. Finally, the comparison between the results of MD and micromechanical analysis shows that as the size of the nanoparticle in nanocomposites increases, the elastic properties of nanocomposites converge with the results obtained by the micromechanical approach.

Facile fabrication of high-strength biocomposite through Mg2+-enhanced bonding in bamboo fiber

The emerging interests in high-performance biocomposites grows significantly driven by their superior environmental sustainability. This study proposes a unique biocomposite strategy by implementing an acetic and ball-milled treatment to disrupt the bamboo cell wall structure, thereby facilitating further processing by effectively increasing the active sites and specific surface area in the bamboo fiber. The fibers are subsequently carboxymethylated to introduce carboxyl groups which facilitate physical bonding between the fibers and Mg2+ ions that are added to the system. These ions form metal-coordination bonds with the carboxyl groups, acting as ion bridges that significantly strengthen the inter-fiber bonding. The resulted biocomposite exhibits impressive mechanical properties, including a high tensile strength (94.24 MPa) and flexural strength (104.14 MPa), not only that, changes in elastic modulus also highlight changes in fiber bonding, the flexural modulus is 21.29 GPa and the tensile modulus is 7.01 GPa. Moreover, it maintains a low water uptake capacity of only 6.8 % despite being submerged for 12 h. The thermal conductivity and fire retardancy have also been improved. The synergic bonding ability between the cellulose and lignin in the fibers, coupled with the glue-free thermoforming process, enhances the material performance and renders it fully recyclable, thus reducing environmental pollution and providing cost-effective engineering materials to society.

Relationship between changes in muscle stiffness after a comfortable massage and the massage pressure

IntroductionThis study aimed to determine whether massage pressure on the target muscles (biceps brachii muscle [BB] and the medial head of the gastrocnemius muscle [MG]) is related to the massage effect (reducing muscle stiffness). MethodNine healthy participants participated in this study. A physiotherapist massaged the upper arms and lower legs of participants on a rigid desk in a laboratory. Massage was delivered for 10 min with a 3-min rest. The shear modulus (i.e., the muscle stiffness), assessed by shear wave elastography, was measured at various time points (before [PRE], immediately after [POST], and 5 [POST-5], 10, 15, and 20 min after the massage). The massage pressure data (N) were obtained only during massage by force plate sensors. ResultsThe BB shear modulus was significantly reduced POST massage. The MG shear modulus significantly reduced POST massage and remained clearly reduced until POST-5. There was a negative correlation between the total massage pressure and the % change in the shear modulus in both muscles. DiscussionSince the spindle (BB) and pennate (MG) muscles have structural differences, our results suggest that these differences may affect the pattern of changes in the shear modulus in response to massage. ConclusionMassage pressure is related to the massage effect (reducing muscle stiffness), and its relationships to POST are not related to the differences in the morphologies of the spindle (BB) and pennate muscles (MG). However, differences in the morphologies of the spindle and pennate muscles may cause differences in the duration of the massage effects.

Fatigue prediction criteria for rubber antivibration design with temperature effects

It is desirable to obtain a fatigue criterion that can be correlated to different rubbers with both mechanical and temperature effects. In this paper, we investigated the possibility to correlate the S-N base function to temperature environments. The published experimental data consisting of temperatures ranging from 23-90 ° C for rubber samples with 50 and 45 Shore hardness were used to validate the existing S-N base function. Concomitantly, a new S-N base function was derived by adding two temperature functions from the test data. In the two criteria, both the thermal expansion and the shear modulus change with temperature were considered. The results have shown that for the 50 Shore hardness rubber, approximately 94% of the points predicted using the existing function and 100% of the points using the new function fell within a scatter band of 1.5. For 45 Shore hardness rubber at 90°C, all predicted points using the existing function fell along the upper limit line of a scatter band of approximately 2.0. On the contrary, all predicted points at 90 ° C fell within a scatter band of 1.35 using the new function. Therefore, this potential novel approach could be used in both the design stages of antivibration rubber components.

Stress relaxation behavior of graphene-epoxy nanocomposites: Effects of graphene fraction, strain levels, and temperature

In this study, strain level and temperature-dependent stress relaxation behavior of neat epoxy and graphene-epoxy nanocomposites are experimentally investigated. Tests are performed for neat epoxy, graphene-epoxy nanocomposite with content of 0.1 wt% and 0.5 wt% graphene. To determine the effect of strain levels on relaxation behavior, three constant strain levels are chosen: 3.16%, 7.15%, and 35.5%. Those strain levels are chosen to correspond to different zones on the stress–strain curves as: viscoelastic region, yield region, and visco-plastic region. Results have shown that with the addition of 0.1 wt% graphene, relaxation modulus improved 41% at 3.16% constant strain. The effect of graphene is less effective at high strain values: for 7.15% strain, the change in relaxation modulus is 32%, and at 35.5 %strain, the change is 21.25%. In addition, tests are performed for three temperature values: 23, 65, and 120°C to determine the effect of temperature on the relaxation behavior. As expected, nano composition has an improved effect on the stress relaxation behavior. An increasing temperature increases the stress relaxation values. At room temperature (for 7.15% constant strain level) the drop in relaxation modulus for neat epoxy is 32%, this value drops to 22% with the addition of 0.1 wt% graphene. At 65°C, the rate of decrease in modulus with the addition of graphene is almost unchanged. At 120°C, the relaxation performance of the material slightly decreases. Therefore, as a novel result of the research, it is determined through the conducted experiments that increasing temperature lessens the improvement effect of graphene composition on the stress relaxation behavior.

Evaluation of drying shrinkage effects on the elastic properties of porous sandstones using a modified micromechanical model

SUMMARY The effects of pore fluids on the elastic properties of sedimentary rocks can be broadly categorized into two mechanisms: variations in pore compressibility due to the physical properties of the fluids and alterations in the stiffness of the rock frame resulting from rock–fluid interactions. Particularly, as rock–fluid interactions alter the stiffness of contacts between mineral grains, changes in the fluid properties around grain contacts can induce volumetric deformation of the rock as well as in variations of the associated elastic coefficients. Even though many previous studies have explored the influence of swelling of clay minerals, the relationships between changes in elastic moduli and deformation in rocks, which hardly contain swellable minerals, remain to date enigmatic. In this paper, to evaluate quantitatively these effects, drying rates, strains and ultrasonic velocities of small cylindrical Berea sandstone samples were measured as their water saturation was decreased by evaporative drying. The measurements clearly showed drying shrinkage and drastic increases in shear and compressional moduli for all the samples under an almost fully dried condition. Previous studies considered that the alteration in surface energy of grain minerals between wet and dry states affected the contact stiffness between them. Some of them used micromechanical model, uniting the Digby grain contact model with the effective medium theory, to interpret the changes in elastic moduli observed in the non-swellable sandstones during water adsorption on the grain surface. The conventional micromechanical model assumes that a mineral grain is a pure sphere and that the number of contacts between the grains is one. However, grains in a sedimentary rock are generally not purely spherical, and the contact surface is composed of several adhesive asperities. We therefore modified the conventional model by introducing the curvature radius and the number of asperities per contact surface. The modified model well reproduced the shear moduli under wet conditions using the strain and moduli measured under dry conditions. On the other hand, the predictions of the compressional moduli using the model were partially in agreement with the experimental results. Therefore, we attempted to qualitatively interpret the relationship by combining the model with the viscoelastic effect associated with wave-induced fluid flow. The deformation and changes in the elastic moduli of rocks resulting from multiple pore fluids within them can be better understood by the present combined model.

Biopolymer composites based on ultrasound/plasma‐modified fiber waste and polybutylene adipate terephthalate (PBAT): Physicochemical and functional performance

AbstractA strategy for a circular economy is to reuse and modify agricultural wastes through green methodologies, which can be employed as high‐value raw materials to produce sustainable materials. Thus, polybutylene adipate terephthalate (PBAT) composites containing plasma (P) and ultrasound/plasma (UP) modified Agave fibers (AF) were prepared by the melt mixing process. The effect of content (10% and 20%) and type of fiber on the processing, structural, mechanical, and water barrier performance of PBAT composites was assessed. FTIR analysis exhibited the presence of O‐Si‐O groups in all composites containing modified AF, indicating the presence of nanometric hexamethyldisiloxane (HMDSO) coating on the fiber surface. The addition of modified AF into PBAT polymer promoted higher torque levels and specific energy during the composites processing, indicating higher matrix‐fiber interactions that hindered the free flow of the polymeric chains. Nevertheless, SEM, DSC, and crystallinity results revealed that PBAT‐AFUP composites exhibited more homogeneous surfaces and ordered structures that promoted higher entanglement between modified fibers and the PBAT matrix and restricted the binding sites for water interaction as changes in tensile modulus (from 90 to 163 MPa) and water contact angle (from 63 to 77°) indicated. These findings show that modified Agave fibers are a sustainable raw material that can be easily processed to produce soft and hydrophobic PBAT‐based composites.Highlights The fiber type determined the functional performance of the PBAT composites The modified fibers increased the entanglement of composite chains UP‐modified fibers improved the water barrier performance of the PBAT matrix

Inhibition of Phlpp1 preserves the mechanical integrity of articular cartilage in a murine model of post-traumatic osteoarthritis

ObjectivePhlpp1 inhibition is a potential therapeutic strategy for cartilage regeneration and prevention of post-traumatic osteoarthritis (PTOA). To understand how Phlpp1 loss affects cartilage structure, cartilage elastic modulus was measured with atomic force microscopy (AFM) in male and female mice after injury. MethodsOsteoarthritis was induced in male and female Wildtype (WT) and Phlpp1-/- mice by destabilization of the medial meniscus (DMM). At various timepoints post-injury, activity was measured, and knee joints examined with AFM and histology. In another cohort of WT mice, the PHLPP inhibitor NSC117079 was intraarticularly injected four weeks after injury. ResultsMale WT mice showed decreased activity and histological signs of cartilage damage at 12 but not 6 weeks post-DMM. Female mice showed a less severe response to DMM by comparison, with no histological changes seen at any timepoint. In both sexes the elastic modulus of medial condylar cartilage was decreased in WT mice but not Phlpp1-/- mice after DMM as measured by AFM. By 6 weeks, cartilage modulus had decreased from 2MPa to 1MPa in WT mice. Phlpp1-/- mice showed no change in modulus at 6 weeks and only a 25% decrease at 12 weeks. The PHLPP inhibitor NSC117079 protected cartilage structure and prevented signs of OA 6 weeks post-injury. ConclusionsAFM is a sensitive method for detecting early changes in articular cartilage post-injury. Phlpp1 suppression, either through genetic deletion or pharmacological inhibition, protects cartilage degradation in a model of PTOA, validating Phlpp1 as a therapeutic target for PTOA.

Effect of sand fineness modulus on SCC and SCLC properties

Due to the advantageous characteristics of self-compacting concrete (SCC) and self-compacting lightweight concrete (SCLC), the advancement of the SCC is considered a remarkable engineering feat. Fine aggregate is one of the main parameters of SCC; however, its effect on SCC and SCLC properties is somewhat restricted due to the limited extent of in-depth research conducted in this field. To investigate the effect of changes in sand fineness modulus (FM) on the fresh and hardened properties of SCC and SCLC, an experimental investigation with twelve mixtures was designed. Two series of mixtures were used, each with two coarse aggregate types: Light Expanded Clay Aggregate (LECA) and natural gravel. Each series included different sand FM (2.3, 2.7, and 3.1), with the incorporation of 7% micro-silica (MS) as a cement substitute in some designs. The fresh properties of the concrete were evaluated through tests such as Slump Flow, V-Funnel, U-box, L-Box, and J-Ring, in addition to the Static Segregation Column test. For evaluating the hardened properties, 192 specimens were tested, including compressive, tensile, and flexural specimens. Moreover, fracture energy was also investigated. As the sand FM increased, it was expected that the fresh properties of concrete might decrease due to coarser aggregate volume, and the hardened properties would improve under these conditions. However, the results showed that increasing the sand FM improved the fresh and hardened properties of SCC and SCLC. By increasing the sand FM, the flowability, filling ability, and passing ability of both concrete improved, but on the other hand, elevating the sand FM reduced the segregation resistance of the mixes. In contrast, the hardened properties exhibited contrasting outcomes with increasing the sand FM. Increasing the sand FM improved the compressive strength by about 20% in both concrete types.

Enabling resonant ultrasound spectroscopy in extreme environments

Elastic moduli are fundamental thermodynamic susceptibilities that connect to thermodynamics, electronic structure, and mechanic properties. Thus, determining the changes of elastic moduli as a function of time or temperature is a powerful tool to study several thermodynamic and materials phenomena. Resonant Ultrasound Spectroscopy (RUS) determines elastic constants with high accuracy and precision from a single measurement of the mechanical resonances of a sample. Conventionally, the quantitative extraction of elastic moduli with RUS assumes free boundary conditions but lead to unstable positioning of the sample making it incompatible with extreme environments like high magnetic fields. In practice, even holding the sample produces contact forces that deviate from free-boundary conditions shifting resonant frequencies. In this talk, we show that, we can reduce the free-boundary conditions (by gluing/adhering the sample to the transducer), while still being able to obtain the full elastic tensor by a simple model of the sample-transducer interaction. This means elastic constants can be determined to within the uncertainty of conventional RUS, but with significant improvements in sample stability and control of sample orientation. We show the results of studying of several magnetic and structural phase-transitions using this new method.

Impact of water absorption on the creep performance of epoxy/microcrystalline cellulose composites

AbstractRecently, considerable effort has been made to study cellulose/epoxy composites. However, there is a gap when it comes to understanding the post‐conditioning anomalous effect of moisture uptake on their mechanical and dynamic‐mechanical properties, and on their creep behavior. In this work, up to 10.0 wt% microcrystalline cellulose (MCC) was incorporated into epoxy resin by simple mixing and sonication. Epoxy/MCC composites were fabricated by casting in rubber silicone molds, and rectangular and dog‐bone test specimens were produced. The moisture uptake, dynamic mechanical, chemical, tensile, and creep behavior were evaluated. The incorporation of MCC increased the water diffusion coefficient. The changes in storage modulus and glass transition temperature, combined with Fourier‐transform infrared spectroscopy analysis, evidenced that water sorption in epoxies causes both plasticization and additional resin crosslinking, although the latter is prevented by the addition of MCC. The creep strain of the composites increased by 60% after conditioning, indicating that plasticization induced by water sorption plays an important role in the long‐term properties of the composites.

Mechanical and Thermal Analysis of Duroplastic Matrix Composites over a Range of Temperatures.

It is commonly acknowledged that polymer composites in service are often subjected to not only intricate mechanical loads but also harsh environmental conditions. The mechanical and thermal properties of five particular composites are explored here. The composites are composed of laminates of glass cloth type "E" sheet infilled with a duroplastic matrix. This is a thermoset polymer-epoxy resin with different molecular weights. The composites were fabricated by IZOERG company, which is based in Poland. The final articles were 1.5 mm thick by 60 cm long and 30 cm wide, with the glass layers arranged parallel to the thickness. Young's modulus and tensile strength were measured at room temperature. Using the thermal analysis of dynamic mechanical properties (DMTA), the values of the storage modulus and the loss modulus were determined, and the damping factor was used to determine the glass transition temperature (Tg). It was revealed that the nature of changes in the storage modulus, loss modulus, and damping factor of composite materials depends on the type of epoxy resin used. Thermal expansion is a crucial parameter when choosing a material for application in cryogenic conditions. Thanks to the TMA method, thermal expansion coefficients for composite materials were determined. The results show that the highest value of the coefficient of thermal expansion leads the laminate EP_4_2 based on brominated epoxy resin cured with novolac P. Duroplastic composites were characterized at cryogenic temperatures, and the results are interesting for developing cryogenic applications, including electric motors, generators, magnets, and other devices.

The relationship between estimates of puborectalis muscle shear modulus made with shear wave elastography and electromyography in healthy men

Aims:This study aimed to: (i) describe a method to estimate shear modulus of the male puborectalis muscle using shear wave elastography (SWE); (ii) investigate the relationship between changes in shear modulus and electromyography (EMG) of the left and right puborectalis muscle; (iii) investigate whether measures made at rest differ before and after a maximal voluntary contraction; and (iv) investigate whether rest measures are consistent over repeated submaximal contractions. Materials and Methods:Eleven males with no history of pelvic symptoms participated. Puborectalis muscle SWE was recorded using a transperineal ultrasound. Puborectalis EMG was recorded with a custom electrode stabilized to the anal mucosa with suction. Measures were made during rest and contractions at 10, 20, 30, 50 and 75% of maximal voluntary contraction (EMG). Correlation between normalized EMG and shear modulus data over contraction intensities was calculated (left; right). Amplitude of each measure at rest was compared before and after maximum voluntary contraction and before each submaximal contraction. Results:Group data showed strong linear correlations between shear modulus and EMG for the left r2=0.71 [SD 0.16; 95% CI 0.61−0.81], and right r2=0.56 [0.33; 0.35−0.76] puborectalis and there was no difference in the correlation between sides (p = 0.17), or between rest values of EMG or shear modulus before and after maximal and submaximal contractions (all p>0.31). Conclusions:This study confirms the viability of SWE for measurement of the puborectalis muscle in men and validity of changes in SWE as an estimate of shear modulus/stiffness of this muscle. Healthy men returned to rest values after contraction at different intensities.

Torsional actuation and vari-stiffness characteristics of SMA/basalt hybrid braided composite tubes

Shape memory alloy hybrid composite (SMAHC) tubes have the potential to actively control the torsional stiffness of structures. In this work, shape memory alloy (SMA) wires orthogonally braided with basalt fiber were heated by electricity to generate the torsional torque of the SMAHC tube, and then the torsional stiffness of the tube was measured. The macroscopic finite element model of the SMAHC tube was established to assist in the analysis of the torsional stiffness variation before and after heating. According to the experimental results, the actuating torsional ability was affected by the phase transformation recovery force of SMA. The active control of torsional stiffness depends on the change of SMA modulus, the magnitude and direction of SMA wires’ recovery force, and the thermodynamic properties of matrix at different temperatures. Then an evaluation model for the torsional stiffness control effect was proposed. These research results can be used for the adaptive control of the stiffness and vibration characteristics of rotating composite structures, and provide a novel and effective method for the control of structural torsional characteristics with lightweight effects.

Thermal effect on the modal characteristics of the rotor of a typical cryogenic turbine for helium refrigerator

The helium turbine is a critical component of the cryogenic system for nuclear fusion devices. To ensure the turbine's steady functioning and avoid resonance, it is essential to understand and clarify the influence of key factors on its modal characteristics. This paper investigates the influence of thermal effect, fluid-structure interaction and rotational effects on the intrinsic frequency of a helium turbine used in the Comprehensive Research Facility for Fusion Technology (CRAFT) cryogenic system. The effects of coefficient of thermal expansion, thermal conductivity and Young's modulus on the intrinsic frequency of the rotor are explored, considering these thermal factors that vary with temperature. The results indicate that thermal effect has the greatest influence on the intrinsic frequency of the rotor over rotational effectand fluid-structure interaction. Lower-order modes are more sensitive to changes in thermal conductivity, while higher-order modes are more sensitive to changes in Young's modulus at low temperatures. The intrinsic frequency of the rotor is more sensitive to changes in the thermal factors of the shaft rather than the thermal factors of the impellers.

Wearable bioadhesive ultrasound shear wave elastography.

Acute liver failure (ALF) is a critical medical condition defined as the rapid development of hepatic dysfunction. Conventional ultrasound elastography cannot continuously monitor liver stiffness over the course of rapidly changing diseases for early detection due to the requirement of a handheld probe. In this study, we introduce wearable bioadhesive ultrasound elastography (BAUS-E), which can generate acoustic radiation force impulse (ARFI) to induce shear waves for the continuous monitoring of modulus changes. BAUS-E contains 128 channels with a compact design with only 24 mm in the azimuth direction for comfortable wearability. We further used BAUS-E to continuously monitor the stiffness of in vivo rat livers with ALF induced by d-galactosamine over 48 hours, and the stiffness change was observed within the first 6 hours. BAUS-E holds promise for clinical applications, particularly in patients after organ transplantation or postoperative care in the intensive care unit (ICU).

Study on cross-section distortion behaviors for tube bending at varying geometry parameters

Tube bending is often plagued by the critical forming defect of cross-section distortion, which increases flow resistance, affects the strength and stiffness of bent-tube, and thus directly influences the usage performance of bent-tube components. To achieve accurate tube bending forming, the distortion behaviors for cross-section during tube bending should be clarified. A finite element (FE) model accounting for the change in elastic modulus was employed for addressing the distortion behaviors of the cross-section for high strength 0Cr21Ni6Mn9N (0Cr21Ni6Mn9N-HS) tube in NC rotary draw bending at varying geometry parameters. The findings indicate that accounting for the change in elastic modulus can aggrandize the distortion ratio of the cross-section, but has no significant impact on the distortion behaviors for the cross-section. The cross-section ovality behaviors of the 0Cr21Ni6Mn9N-HS tube at various geometry parameters for NC rotary draw bending have been clarified, and the ranges of sound parameters have been determined.

Homogenization of Inconel 625 based periodic auxetic lattice structures with varying strut thickness

The purpose of this research is to analyze the mechanical behavior of auxetic re-entrant-based metamaterials with properties similar to Inconel 625 using homogenization techniques. Through a thorough analysis, this study investigates the displacement patterns exhibited in various materials throughout a range of thicknesses. The examination also includes analyzing how the Young’s modulus changes with varying strut thickness after homogenization. This detailed investigation provides information on the stiffness and deformation response of the material. This research advances our knowledge of the complex mechanical properties of re-entrant-based auxetic metamaterials that resemble Inconel 625 by interpreting these displacement and Young’s modulus patterns.

Changes in Milk Protein Functionality at Low Temperatures and Rennet Concentrations

This study aimed to evaluate the influence of low-concentration rennet on the chemical, rheological characteristics, and protein fractions of skim milk (SM) at 4 ± 1 °C. Skimmed milk (SM) was divided into four lots of 500 mL, and diluted rennet (1:10,000) was added at different levels at 4 ± 1 °C. The treatments included control (no rennet), T1 (0.001 mL/rennet), T2 (0.01 mL rennet), and T3 (0.1 mL rennet) treatments, which were incubated for 24 h. The sampling was performed at 0, 1, 2, 6, 12, and 24 h, and the SM after incubation time was heated to 73 °C/16 s to denature the rennet enzyme. Skim milk samples (SMS) (control and rennet-added samples) were evaluated for proximate composition, capillary gel electrophoresis (CGE), hydrodynamic diameter, zeta potential, and rheology at 0, 1, 2, 6, 12, and 24 h. Foaming ability, foaming stability, water-holding capacity (WHC), oil emulsifying activity (OEA), and emulsion stability (ES) were performed at 0, 12, and 24 h of incubation time. There was a significant (p < 0.05) increase in non-proteins by 0.50% and in non-casein nitrogen by 0.81% as incubation progressed. The results showed that aggregation or curd was not formed during storage time. The CGE data indicated that increasing the rennet concentration had a significant (p < 0.05) effect on decreasing κ-CN, and breakdown increased at higher levels of rennet usage. There was a significant (p < 0.05) increase in the hydrodynamic diameter and a decrease in the zeta potential values in rennet-added samples at the end of the incubation time (24 h). The rheological results showed no changes in the storage modulus (G′), loss modulus (G″), or viscosity values. Increasing the rennet amount and storage time led to a significant (p < 0.05) decrease in the foaming ability and foaming stability and a significant (p < 0.05) increase in the oil emulsifying activity and emulsion stability of rennet-added SMS. This study concluded that milk protein functionality can be changed without aggregating or curd formation, and rennet milk can be processed.

Tendon biomechanical properties are altered by storage duration but not freeze-thaw temperatures or cycles.

Tendon allograft and xenograft processing often involves one or more steps of freezing and thawing. As failure strength is an important graft consideration, this study aimed to evaluate effects on failure properties when varying freeze-thaw conditions. Kangaroo tendons, a potential xenograft source, were used to evaluate changes in ultimate tensile strength (UTS), failure strain and elastic modulus after exposure to different freezer-storage temperatures (-20°C vs.-80°C), storage durations (1, 3, 6, 9, or 12 months), number of freeze-thaw cycles (1, 2, 3, 4, 5, or 10), or freeze-thaw temperature ranges (including freezing in liquid nitrogen to thawing at 37°C). Tendons stored for 6 or more months had significantly increased UTS and elastic modulus compared with 1 or 3 months of storage. This increase occurred irrespective of the freezing temperature (-20°C vs.-80°C) or the number of freeze-thaw cycles (1 vs. 10). In contrast, UTS, failure strain and the elastic modulus were no different between storage temperatures, number of freeze-thaw cycles and multiple freeze-thaw cycles across a range of freeze and thaw temperatures. Common freeze-thaw protocols did not negatively affect failure properties, providing flexibility for graft testing, storage, transportation and decellularisation procedures. However, the change in properties with the overall storage duration has implications for assessing the consistent performance of grafts stored for short versus extended periods of time (<6 months vs. >6 months), and the interpretation of data obtained from tissues of varying or unknown storage durations.