We developed a genetically encoded homoFRET biosensor to measure cellular nicotinamide adenine dinucleotide phosphate redox state (Apollo-NADP+). This sensor is based on the homo-dimerization of glucose-6-phosphate dehydrogenase and responds to NADPH/NADP+ redox state with changes in steady-state fluorescence anisotropy associated with homologous Forster resonance energy transfer (homoFRET). We now aim to use this sensor in a tissue. However, tissue scatter has a significant affect on steady-state anisotropy imaging, which may prohibit use of the sensor. To determine whether Apollo-NADP+ is suited for imaging in 3D tissues, we are investigating the effect of scatter on fluorescence anisotropy using tissue phantoms and living tissue. Thus far, we imaged monomeric and tandem-dimer fluorescent protein constructs suspended in a light scattering solution and showed these controls can be distinguished despite a significant affect on their fluorescence intensity. We also expressed our biosensor in living neural-progenitor cell colonies to discern whether stimulant responses in 3D cell cultures are consistent with those observed in monolayer cell cultures. We will subsequently explore the use of our biosensor in in vivo tissue imaging by expressing our sensor in zebrafish. Overall, this project aims to determine whether anisotropy-based sensors such as Apollo-NADP+ can be translated to study living tissue.