Objective: To evaluate, by diaphanization, the cleaning ability three different instruments on walls of flattened root canals. Methods: Thirty lower incisors, which had their root canals filled with India ink after coronal access and determination of the working length, were selected. The samples were randomly divided into three groups, of 10 teeth each, according to the instrument used during preparation: Group 1– hand files instrumentation; Group 2– instrumentation with ProTaper Universal rotary files; Group 3 – instrumentation with iRace rotary files. After instrumentation, the teeth were diaphanized and evaluated for cleaning ability, by analyzing the amount of dentin walls in which Indian ink was not removed. Results: When performing the Kruskal-Wallis test (p>0.05) there were not significant statistical differences between groups, as well as for the arithmetic average of the scores at cervical, middle and apical thirds. Conclusion: None of the instruments used for root canal preparation of flattened root canals was able to perform a complete cleaning of the dentin walls.