Cerdocyon Research Articles

The behavioral response of the crab-eating fox to olfactory enrichment

The effect of olfactory stimuli as a means of environmental enrichment is underexplored in canids. The crab-eating fox is the most abundant wild canid in zoos, but few studies have addressed the reaction of this species to olfactory cues. This study sought to investigate the reaction to olfactory stimuli in crab-eating fox with the aim of increasing their well-being. Four potentially attractive olfactory stimuli (meat, cheese, rat urine, and egg) were presented outside the enclosures in five-minute sessions. The reactions of the crab-eating foxes were filmed and analyzed using the focal animal method. All behaviors were recorded pre (Basal), during (Exp), and post (Pos) stimulus. Behavioral responses were classified as positive (P +), negative (N-), and other (Ot). The average times recorded for each category of behavior in each phase were analyzed. Olfactory stimuli significantly increased P + responses by up to 18.2 times (p < 0.001) during Exp, concomitant with a 2.3-fold increase in N- responses (p < 0.03). The category of Ot behaviors declined from the Basal (p < 001) for the Exp and remained low in the Pos (p < 001) in relation to the Basal. Only the category of P + behaviors remained increased (p < 0.001) in relation to the Basal, while the category of N- behaviors returned to baseline values (p > 0.05) after the withdrawal of the stimulus. Olfactory enrichment, as investigated in other species of mammals, was effective and led to a sustained increase in well-being, but the exposure time must be balanced so as not to elicit negative behaviors. The method used is innovative, low cost, and flexible. This is the first published study of olfactory enrichment for crab-eating foxes.

Occurrence of antibodies to Leptospira spp and Toxoplasma gondii in captive wild animals in the Zoobotanical Park of Petrolina, PE, Brazil

OBJECTIVE: Leptospirosis and toxoplasmosis are cosmopolitan zoonoses affecting domestic and wild animals that can inhabit urban environments acting as reservoirs of infectious agents, increasing the risk of zoonotic diseases transmission to humans. The present study aimed to detect serum antibodies to Leptospira spp. and Toxoplasma gondii in captive wild animals from the Zoobotanical Park, located in the city of Petrolina, State of Pernambuco, Brazil. METHODS: Samples were collected from 72 wild animals, including 12 mammals, 26 birds, and reptiles. Serological diagnosis for anti-T. gondii antibody detection was performed on birds and mammals using the modified agglutination test (MAT Toxo). Anti-Leptospira spp. antibodies were detected in reptiles and mammals by microscopic agglutination test (MAT Lepto). RESULTS: The occurrence of T. gondii antibodies was 56.4% (22/39), with positive animals from the genera: Procyon cancryvorus (1/2), Ara ararauna (4/4), Patagioenas picazuro (5/5), Amazona aestiva (5/5), Aratinga acuticaudata (3/3), Tayassu tajacu (2/2), Nasua nasua (1/1) and Cerdocyon thous (1/1). Anti-Leptospira spp. antibodies were found in 4.4% (2/45) of the animals, with one out of the two Tamandua tetradactyla positive for the subgroup Australis and one out of the 23 Geochelone carbonaria positive for the subgroup Hebdomadis. CONCLUSION: Captive wild animals from zoos can take part in the life cycle of these agents, acting as reservoirs, thus assuming an important role in the epidemiological chain of these important zoonotic agents.

Molecular eco-epidemiology of Paracoccidioides brasiliensis in road-killed mammals reveals Cerdocyon thous and Cuniculus paca as new hosts harboring this fungal pathogen.

Wild animals infected with Paracoccidioides brasiliensis represent important indicators of this fungal agent presence in the environment. The detection of this pathogen in road-killed wild animals has shown to be a key strategy for eco-epidemiological surveillance of paracoccidioidomycosis (PCM), helping to map hot spots for human infection. Molecular detection of P. brasiliensis in wild animals from PCM outbreak areas has not been performed so far. The authors investigated the presence of P. brasiliensis through nested-PCR in tissue samples obtained from road-killed animals collected nearby a human PCM outbreak spot, Rio de Janeiro state, Brazil and border areas. Eighteen species of mammals were analyzed: Dasypus novemcinctus (nine-banded armadillo, n = 6), Cerdocyon thous (crab-eating fox, n = 4), Coendou spinosus (hairy dwarf porcupine, n = 2), Lontra longicaudis (Neotropical river otter, n = 1), Procyon cancrivorus (crab-eating raccoon, n = 1), Galactis cuja (lesser grison, n = 1), Tamandua tetradactyla (collared anteater, n = 1), Cuniculus paca (paca, n = 1), and Bradypus variegatus (brown-throated three-toed sloth, n = 1). Specific P. brasiliensis sequences were detected in the liver, spleen, and lymph node samples from 4/6 (66.7%) D. novemcinctus, reinforcing the importance of these animals on Paracoccidioides ecology. Moreover, lymph nodes samples from two C. thous, as well as lung samples from the C. paca were also positive. A literature review of Paracoccidioides spp. in vertebrates in Brazil indicates C. thous and C. paca as new hosts for the fungal pathogen P. brasiliensis.

Detection of anti-Toxoplasma gondii antibodies in wild free-living birds and mammals from the northwest region of São Paulo state, Brazil

Toxoplasmosis is a protozoonosis caused by an obligate intracellular parasite named Toxoplasma gondii, which can infect humans and a large number of homeothermic animal species with worldwide distribution. The present study aimed to detect anti T. gondii antibodies from serological samples of free living wild animals from the northwest region of São Paulo state, Brazil. Thirty-two samples (eight from birds and 24 from mammals) were analyzed by the modified agglutination test (MAT) using 5 cut-off points for birds and 25 for mammals. Seropositivity was observed in 25% (2/8) of birds, including the species Rupornis magnirostris (roadside hawk) and Caracara plancus (southern caracara), and 29.2% (7/24) animals were seropositive among mammals, including one hoary fox (Lycalopex vetulus), two maned wolves (Chrysocyon brachyurus), one black howler monkey (Alouatta caraya), two crab-eating foxes (Cerdocyon thous) and one gray brocket deer (Mazama gouazoubira). The results obtained with the present study indicate the exposure to T. gondii of free-living wild animals from the northwest region of São Paulo state and, therefore, that they probably play a role in the transmission and maintenance of T. gondii in the environment they inhabit. Thus, identification of the infection in several animal species in the region indicates the environmental contamination of the area. Studies of this nature may help to understand the importance of the prevention and control of this disease in Brazil.

Analysis of conflicts with wild carnivores in the Humid Chaco, Argentina

Interactions between humans and carnivores tend to be conflictual, especially due to predation on domestic animals. As certain landscape characteristics predispose the occurrence of carnivore attacks, spatial modelling of predation events can be particularly useful when developing management plans. In this study we determined the incidence of predation on the mortality of domestic animals by interviewing local inhabitants. In addition, we identified the spatial variables that explain the distribution of the conflicts and we created a two–scale model based on the Maxent algorithm. The results showed that Puma concolor (41.2 %) and the foxes Lycalopex gymnocercus and Cerdocyon thous (33.3 %) were the most conflictive species. Predation accounted for only 5.6 % of the causes of domestic animal mortality. The distribution models showed that the most probable variables for predicting conflicts were the distance from the roads, livestock density and the proportion of anthropized areas. High–risk areas represented 28 % of the study area and were distributed in broad patches around the protected areas and in the eastern sector of the area.

First record of plastic ingestion by Cerdocyon thous (Carnivora, Canidae) in northeastern Brazil

Notas sobre Mamíferos Sudamericanos es una revista de la Sociedad Argentina para el Estudio de los Mamíferos (SAREM). Publica información acotada donde se describen nuevas localidades de registro, datos sobre comportamiento y aspectos ecológicos de mamíferos neotropicales.

Rangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay

Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5–100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9–100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown.

Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology.

Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as definitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identification of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox = 46, crab-eating fox = 55) and feces (pampas fox = 84, crab-eating fox = 2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplification of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel real-time PCR TaqMan™ probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the first report of N. caninum DNA in pampas fox feces (n = 1), thus it was also detected from pampas fox tissues (n = 1). Meanwhile, T. gondii was found in tissues of pampas (n = 1) and crab-eating (n = 1) foxes and H. triffittae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.

Origin and main ramifications of coeliac artery in Cerdocyon thous.

Cerdocyon thous is the canid with the greatest geographical coverage in South America. The aim of this study was to describe the origin, skeletopy, length and main branches of the coeliac artery in C. thous. The dissections were performed on 14 cadavers of adult specimens, 6 males and 8 females, with a rostrosacral length average of 67.00 ± 4.7 cm and 62.09 ± 5.7 cm, respectively. The specimens were collected dead on highways on the banks of the Atlantic Forest (Rio de Janeiro) and the Pampa biome (Rio Grande do Sul) in Brazil. The cadavers were fixed and preserved in a formaldehyde solution until dissection. The coeliac artery was dissected, the length was measured "in situ" and its main branches were recorded. The coeliac artery emerged as a single artery in all dissected animals. The average length of the coeliac artery was 1.43 ± 0.17 cm in males and 1.39 mm ± 0.24 cm in females, with no significant difference in this measurement between sexes. The predominant skeletopy was at the level of the second lumbar vertebra (57.1%), positioned on average 1.43 cm cranially to the cranial mesenteric artery. In most individuals (92.9%), the classic trifurcation was formed: the coeliac artery branched into the hepatic, left gastric, and lienal arteries. Only 1 male animal presented a bifurcation formed between the hepatic artery and a gastrolienal trunk. These anatomical characteristics are similar to those of other species of the Canidae family, possibly due to their phylogenetic proximity.

Rangelia vitalii molecular and histological quantification in tissues comparing crab-eating foxes (Cerdocyon thous) and domestic dogs

Rangeliosis is a condition transmitted by the tick Amblyomma aureolatum and caused by the protozoan parasite Rangelia vitalii in canids. In domestic dogs, the disease causes a severe hemolytic disease, while in wild canids the piroplasm is often detected without any clinical abnormality. This study aimed to detect and quantify the number of copies of the R. vitalii Hsp70 gene (indirect parasite burden) in several organs of domestic and South American wild canids (Cerdocyon thous and Lycalopex gymnocercus) to elucidate distinct clinical presentations of rangeliosis in these species. A total of seven domestic dogs that died due to rangeliosis and 38 wild foxes were initially included, with all dogs presenting histological and molecular features of rangeliosis, while eight C. thous were positive at the molecular analysis for R. vitalii. Fragments of 22 organs collected from domestic (n = 7) and wild foxes (n = 8) were employed for histological and molecular quantification using real-time polymerase chain reaction aiming at the Hsp70 gene. Histologically, parasitophorous vacuoles were constantly detected in the dogs, while these were detected only in two C. thous. Parasitic burden was significantly higher in the digestive, cardiorespiratory, endocrine, genitourinary, and skeletal-muscle systems of domestic dogs when compared to wild foxes. In the hematopoietic system of wild canids, some organs, such as the lymph nodes and tonsils, presented significantly lower amounts of R. vitalii, while other organs (spleen, bone marrow, and blood) had results similar to those of domestic dogs. Additionally, the central nervous system of both domestic and wild canids presented a similar quantity of R. vitalii. The etiological agent is possibly maintained through an asexual reproductive process (merogony) in both domestic and wild species. Nonetheless, a limited or short-duration schizogony phase occurs in C. thous, which would designate this species as a possible reservoir host for the agent. Dogs, in contrast, would most likely act as accidental hosts, presenting a severe and more pathogenic schizogony phase, resulting in characteristic clinical and pathological rangeliosis.

Morphological and molecular diagnosis of diseases of free-ranging crab-eating foxes (Cerdocyon thous)

ABSTRACT The aim of this study was to investigate the occurrence of diseases in free-ranging wild canids that were roadkill on highways in the State of Espírito Santo, Brazil. PCR tests were performed for the detection of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. in the spleen. Morphological evaluation and identification of parasites was performed in the liver and lung. Twenty specimens of C. thous were necropsied at the Animal Pathology Sector of the Veterinary Hospital of the Universidade Federal do Espírito Santo. Tissue samples were processed for histopathological examination and polymerase chain reaction (PCR) analysis. There was no PCR amplification of genomic DNA sequences of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. using DNA extracted from the spleen as template. Histologically, lesions associated with parasitism by Platynosomum sp. and Angiostrongylus sp. were observed in the liver and lung, respectively. This is the first report of Platynosomum sp. and Angiostrongylus sp. parasitism in C. thous in the state of Espírito Santo, Brazil. Therefore, this study demonstrated parasitism of crab-eating foxes by Platynosomum sp. and Angiostrongylus sp. Importantly, no evidence of infection with Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. was obtained by PCR analysis.

Crithidia mellificae infection in different mammalian species in Brazil.

Crithidia mellificae, a monoxenous trypanosomatid considered restricted to insects, was recently reported to infect a bat. Herein, C. mellificae has been demonstrated to have a wider range of vertebrate hosts and distribution in Brazilian biomes than once thought. Parasites isolated from haemocultures were characterized using V7V8 SSU rDNA and glyceraldehyde 3-phosphate dehydrogenase genes. Coatis (Nasua nasua) in the Cerrado; marmosets (Callithrix sp.) and bats (Carollia perspicillata, Myotis lavali, M. izecksohni, Artibeus lituratus) in the Atlantic Forest; crab-eating foxes (Cerdocyon thous) and ocelot (Leopardus pardalis) in the Pantanal biomes were infected by trypanosomatids that displayed choanomastigote forms in haemoculture in Giemsa-stained slide smears. Molecular characterization and phylogenetic inference confirmed the infection of C. mellificae in these animals. Moreover, slight differences in C. mellificae sequences were observed. Crithidia mellificae growth curves were counted at 27°C, 36°C and 37°C, and the morphotypes were able to grow and survive for up to 16 days. Serological titers for C. mellificae were observed in nonhuman primates, demonstrating that this parasite is able to induce a humoral immune response in an infected mammal. These results showed that host specificity in trypanosomatids is complex and far from understood.

A neotropical canid as seed disperser in semiarid areas of Brazil

Seed dispersal consists of diaspore removal from parental plants and relocation to new locations where seeds can germinate and grow seedlings. Seeds that passage through an animal's digestive tract may have changes in percentages, speed, and synchronization of germination. Cerdocyon thous is an omnivorous canid from South America and we investigated its role as a dispersing agent, in semiarid areas, by analyzing feces in three areas of northeastern Brazil. Seeds found in feces and removed directly from fruit (control) had their viabilities evaluated in germination tests, where germinability, germination time of 50% of viable seeds (t50), the emergence velocity, and synchronization indices were determined. Of the evaluated species, the cactus Pilosocereus gounellei showed differences in the parameters between treatments. The passage of seeds of P. gounellei through the canid digestive tract decreased germinability and the germination speed and increased the t50. For the other species, there were no difference between treatments. Therefore, C. thous influenced the germination success and pattern of this cactus, although it did not influence the reproductive behavior of most species. This canid was considered an effective seed disperser in semiarid areas because it moved seeds from the parental plant to sites suitable for its germination.

Diaphragmatic hernia and unilateral renal agenesis in a crab-eating fox (Cerdocyon thous)

An approximately 3-month-old crab-eating fox (Cerdocyon thous) was found by environmental authorities in the State of Paraiba, Northeastern Brazil and referred to a wildlife care center. The fox was presenting respiratory distress and it was referred to the Veterinary Hospital of the Federal University of Paraiba (UFPB) for ancillary testing. Abdominal and thoracic ultrasound and radiographies were performed. These imaging tests indicated the fox had a possible diaphragmatic hernia and ectopic kidney. The imageology results were confirmed on necropsy, which revealed a postero-lateral focal discontinuity of the dorsal aspect of the diaphragmatic muscle with protrusion of the gastrointestinal tract into the thoracic cavity. The stomach and intestinal loops were filled with gas and obliterated the visualization of the heart and lungs. Additionally, only the right kidney was found, and no vestigial left kidney was identified. Congenital diaphragmatic hernias are not commonly observed in wildlife but should be considered as a potential differential diagnosis for acute onset of respiratory distress in young carnivores.

Molecular phylogenetic study in Spirocercidae (Nematoda) with description of a new species Spirobakerus sagittalis sp. nov. in wild canid Cerdocyon thous from Brazil.

The nematode family Spirocercidae Chitwood and Wehr, 1932, comprises three subfamilies, Spirocercinae Chitwood and Wehr, 1932; Ascaropsinae Alicata and McIntosh, 1933; and Mastophorinae Quentin, 1970, which occur worldwide. Spirocercids infect canids and can cause severe illness. The crab-eating fox, Cerdocyon thous (Linnaeus, 1766), is a canid that inhabits most of South America, including Brazil and is a host for several parasitic worms, in particular, nematodes. However, few reports or genetic data are available on the spirocercids found in this host. In the present study, we describe a new species of Spirobakerus Chabaud and Bain 1981, from the intestine of two crab-eating foxes from two different biomes in Brazil. Spirobakerus sagittalis sp. nov. presents a) unequal spicules, with a long, thin left spicule with a lanceolated shape at the tip; b) a pair of sessile papillae and a median unpaired papillae located anteriorly of the cloaca, and c) a tuft without spines at the tip of the tail in females. The molecular phylogenetic analysis indicated that S. sagittalis sp. nov. is basal to the other species of the subfamily Ascaropsinae, which was not recovered as monophyletic. Our phylogenies also indicated that Spirocercidae is paraphyletic, given that Mastophorinae did not group with Ascaropsinae and Spirocercinae. We provide the first molecular data on the genus Spirobakerus and expand the molecular database of the spirocercids. However, further studies, including the sequences of other spirocercid taxa, are still needed to infer the relationships within this family more accurately.

“When the felid’s away, the mesocarnivores play”: seasonal temporal segregation in a neotropical carnivore guild

Interspecific competition within a carnivore guild can result in segregation along dietary, spatial, and temporal scales. Species interactions and resulting avoidance behavior can change seasonally as landscape features and resource abundance may fluctuate. In this study, we examined a carnivore guild in the Pantanal wetland of Brazil to determine whether temporal niche partitioning was a mechanism for coexistence, and if this differed between the wet and dry season. We used camera trapping data to fit kernel density functions of time observations for five species of carnivores to determine activity patterns. We calculated the coefficient of overlap between all species-pair’s activity patterns. Our results found support for temporal segregation among this carnivore guild, with stronger segregation evident during the dry season. Jaguars and pumas showed large overlap in activity in both seasons, while all three mesocarnivores (ocelot, tayra, and crab-eating fox) showed temporal avoidance toward pumas. Mesocarnivores displayed segregating temporal patterns between pairs in both seasons. Temporal segregation is a mechanism for coexistence within this carnivore guild, suggesting increased competition between species especially during the dry season. To maintain carnivore populations, a broader knowledge of interspecific interactions and how this may affect species, utilization or avoidance of habitats is needed. Given the complexities of interspecific interactions among carnivores, conservation efforts should address the needs of the entire guild rather than focus on a single species.

Evaluation of hip joint laxity in crab-eating foxes (Cerdocyon thous)

ABSTRACT: Like canids, crab-eating foxes may probably be predisposed to similar orthopedic diseases of domestic dogs, such as hip dysplasia. However, for the adequate hip dysplasia diagnosis in wild animals, the normality characteristics of each species must be determined. This study aimed to estimate radiographic and computed tomographic (CT) values of hip joint laxity in healthy crab-eating foxes. Fifteen intact crab-eating foxes, eight males and seven females, ages 1 to 5 and mean body mass of 6.66kg were used. Norberg angle (NA) was calculated from ventrodorsal hip-extended radiographs. To calculate the dorsolateral subluxation (DLS) score, the center distance (CD) index, the lateral center edge angle (LCEA), and the dorsal acetabular rim angle (DARA), measurements obtained from transverse CT images were used. No statistically significant differences were observed between the right and left sides in the radiographic and tomographic parameters. The mean NA was 107.57°. The mean DLS score, the CD index, the LCEA, and the DARA were 60.79%, 0.16, 98.25° and 13.47°, respectively. The data obtained are helpful in characterizing mean values of the hip joint in healthy crab-eating foxes, and can contribute to the knowledge of the species.

Neotropical Carnivores: A Photo Gallery of the Data Set on Carnivore Distribution in the Neotropics

Photo 1. A Neotropical otter (Lontra longicaudis; Mustelidae) in Rio Negro, Mato Grosso do Sul state, Brazil. This semi-aquatic mustelid is threatened due to habitat destruction along rivers and coastal areas. This species has 1,979 records across nine countries in our data set. Photo credit: Júlia Emi F. Oshima. Photo 2. A young South American coati (Nasua nasua; Procyonidae) climbing a tree in Matão, São Paulo state, Brazil. This species occurs widely in South America, being found in anthropogenic and pristine landscapes. This species has 7,045 records across nine countries in our data set. Photo credit: Júlia Emi F. Oshima. Photo 3. A crab-eating fox (Cerdocyon thous; Canidae) in Miranda, Mato Grosso do Sul state, Brazil. This species is a habitat generalist with a wide geographic range. The crab-eating fox was the species with the largest number of records in our data set, with 11,556 records across seven countries. Photo credit: Júlia Emi F. Oshima. Photo 4. An ocelot (Leopardus pardalis; Felidae) in an agricultural landscape in the Southern Brazilian Amazon. This mesopredator was the most recorded felid in our data set with 9,889 records across 18 countries. Photo credit: Francesca Belem Lopes Palmeira. Photo 5. A jaguar (Panthera onca; Felidae) in an agricultural landscape in the Southern Brazilian Amazonia. This apex predator is the largest felid in the Neotropics. This species was recorded in 19 countries and accounted for 6,450 of the records in our data set. Photo credit: Francesca Belem Lopes Palmeira. These photographs illustrate the article “Neotropical Carnivores: a data set on carnivore distribution in the Neotropics” by Nagy-Reis et al. published in Ecology. https://doi.org/10.1002/ecy.3128

Long bone fractures in Cerdocyon thous: macroscopic and microstructural evaluation

Abstract The aim of the present study was to perform the macroscopic and microstructural morphological classification of long bone fractures of Cerdocyon thous. Eighteen cadavers of the species were necropsied, and subjected to radiographic and microscopical evaluation when long bone fractures were detected. Among the 18 cadavers, eight (44%) had fractures equally distributed (33.33%) in the femur, humerus, or tibia. More frequently (61.54%), the fractures were simple and affected the diaphysis, and in smaller proportions (23.08%) reached the physeal line. In diaphyseal and metaphyseal fractures, microscopical evaluation revealed cortical bone tissue, with longitudinal osteons that contained longitudinal and intermediate collagen fibres and lamellae with a delamination aspect. On the other hand, in epiphyseal fractures, trabecular bone tissue was more frequently observed, consisting of trabeculae with disorganised collagen fibres and absence of osteons. In both cases low activity, osteocytes, and low coverage of osteoblasts on the bone surface were noted. It was concluded that the frequency of fractures in the long bones of C. thous was 44%, with females being more predisposed. The findings support the hypothesis that fractures in such animals are caused by being run over by automobiles. The present study contributes significantly in alerting clinicians and surgeons to the types of fractures that C. thous is more predisposed to, its places of greatest occurrence, and its microstructure. Thus, there is a need for joint actions aimed at reducing the number of cases of wild animals being run over by automobiles.

Medium and large-sized mammals in dry forests of the Colombian Caribbean

Mammalian populations are fundamental at functional and ecological levels within ecosystems and are inconstant threat of reduction world wide and in the Neotropic. We evaluated population variables, habitat use, and threats to medium and large mammals (weight &gt; 1 kg) in the terrestrial part of the Tayrona National Natural Park (Santa Marta-Colombia). We carried out in direct annual observations with camera traps between 2012 and 2017, both in rainy and dry seasons. We estimated the richness, relative abundance (RAI), and functional diversity of the mammals present in the area. We recorded 15 species, distributed in seven orders (Carnivora, Rodentia, Pilosa, Cingulata, Lagomorpha, Didelphimorphia, and Cetartiodactyla), 11 families and 13 genera. The species with the highest capture frequency was Dasyprocta punctata (142, RAI = 0.85), followed by Cuniculus paca (56, RAI = 0.33), Odocoileus virginianus (51, RAI = 0.30), and Cerdocyon thous (41, RAI = 0.24). We observed a marked use and preference of habitat in the seasonal dry ever green forest. This finding coincides with the highest value of functional richness (S1= 293.88) for this ecosystem, probably due to its features, such as green vegetation and abundant water streams. Our results reveal stability of the species richness across the years studied, suggesting good health of the mammal populations in the studied area.