Speed Centrifugation Research Articles

Optimization of Centrifugation Speed and pH in Extraction of Uricase Enzyme from Goat Liver

The human body does not have an enzyme that can break down uric acid, so the accumulation of uric acid can cause disease. This problem can be overcome by uricolytic therapy by utilizing the activity of the uricase enzyme. In this study, the uricase enzyme was extracted from goat liver by optimizing the centrifugation speed and the extraction pH. The purpose of the optimization is to get maximum uricase activity. Uricase extraction to optimize centrifugation speed using borate buffer pH 8.5 then centrifuged at 7.000; 9.000; 11.000; 13.000 and 15.000 rpm with a temperature of 4oC. Furthermore, pH optimization was carried out using pH buffers 6, 7, 8, 9, 10 and 11 by centrifuging the optimum speed obtained. The crude extract obtained was further tested for its enzyme activity . The results showed that the highest uricase activity was achieved if the extraction was carried out at pH 8 using centrifugation at an optimum speed of 13,000 rpm. The higher uricase activity indicates that the extracted uricase concentration is increasing.

Pre-analytical processing of plasma and serum samples for combined proteome and metabolome analysis.

Metabolomic and proteomic analyses of human plasma and serum samples harbor the power to advance our understanding of disease biology. Pre-analytical factors may contribute to variability and bias in the detection of analytes, especially when multiple labs are involved, caused by sample handling, processing time, and differing operating procedures. To better understand the impact of pre-analytical factors that are relevant to implementing a unified proteomic and metabolomic approach in a clinical setting, we assessed the influence of temperature, sitting times, and centrifugation speed on the plasma and serum metabolomes and proteomes from six healthy volunteers. We used targeted metabolic profiling (497 metabolites) and data-independent acquisition (DIA) proteomics (572 proteins) on the same samples generated with well-defined pre-analytical conditions to evaluate criteria for pre-analytical SOPs for plasma and serum samples. Time and temperature showed the strongest influence on the integrity of plasma and serum proteome and metabolome. While rapid handling and low temperatures (4°C) are imperative for metabolic profiling, the analyzed proteomics data set showed variability when exposed to temperatures of 4°C for more than 2h, highlighting the need for compromises in a combined analysis. We formalized a quality control scoring system to objectively rate sample stability and tested this score using external data sets from other pre-analytical studies. Stringent and harmonized standard operating procedures (SOPs) are required for pre-analytical sample handling when combining proteomics and metabolomics of clinical samples to yield robust and interpretable data on a longitudinal scale and across different clinics. To ensure an adequate level of practicability in a clinical routine for metabolomics and proteomics studies, we suggest keeping blood samples up to 2h on ice (4°C) prior to snap-freezing as a compromise between stability and operability. Finally, we provide the methodology as an open-source R package allowing the systematic scoring of proteomics and metabolomics data sets to assess the stability of plasma and serum samples.

Measurement of DIC acquisition and evidence for a CO2 concentrating mechanism in Gephyrocapsa oceanica (Isochrysidales, Coccolithophyceae)

ABSTRACT The possession of a carbon concentrating mechanism (CCM) by a given phytoplankton species will in part determine its response to changes in the dissolved inorganic carbon concentrations in the global ocean. Earlier work on CCMs in the coccolithophore Emiliania huxleyi suggested that this species did not possess a CCM, though more recent studies indicate that a CCM is indeed present. Here we report work showing that another coccolithophore species, Gephyrocapsa oceanica, also possesses a functioning CCM, implying that increasing CO2 concentrations are unlikely to lead to enhanced growth rates. We discuss the protocol required for working with this species, noting that due to its liths being 10 or more times as massive as of those of Emiliania huxleyi, much lower centrifugation speeds (<400 × g) must be used to avoid damage to the cells.

Production and Characterization of Nano-Chitosan from Blood Clamshell (Anadara granosa) by Ionic Gelation

Nano-chitosan can be produced from blood clams (Anadara granosa) because they contain 14-35% of chitin. The production of nano-chitosan can be conducted by a bottom-up process using sodium tripolyphosphate (Na-TPP). The aims of this study are to produce nano-chitosan from blood clamshell and to study the factor affecting the particle size of nano-chitosan such as the ratio of chitosan to Na-TPP solution (v/v) and rotation speed of the centrifuge. The research shows that The chitin content on blood clamshell is 25.42%. The yield of chitosan from chitin is about 80.92%. The degree of deacetylation of chitin from blood clamshell reaches 63.18%. The effect of the ratio of chitosan to Na-TPP solution (R) and the rotation speed of centrifuge (N) on the particle size of nano-chitosan can be expressed by equation dp = 0.12 (R)0.714 (N)0.99.

Micellar solutions of Pluronic F127 for ocular drug delivery

AbstractThe goal of this research was to prepare drug‐encapsulated polymeric micelles for delivery to the posterior segment of the eye. Solutions of surface active PF127 at the critical micellar concentration (CMC) were prepared in deionized water (DI water) and phosphate buffer (PBS). The CMC of PF127 solution in DI water and PBS solution were found to 0.21 ± 0.04 and 0.16 ± 0.05% (w/v), respectively. In addition, the micellization of the PF127 was observed around 25 and 21°C in DI water and PBS solution, respectively by DSC analysis. The hydrodynamic diameter of the PF127 micelles were found to range between 3 and 4 nm with polydispersity indices ranging between 0.35 ± 0.22 and 0.96 ± 1.52.Curcumin (CUR), a natural dietary compound having an anti‐VEGF properties, was successfully loaded into PF127 copolymers by the thin‐film hydration method. The CUR‐loaded PF127 micelles were confirmed by several characterization techniques including DLS and HPLC. A detailed study of the effect of experimental conditions on both the particle size and drug encapsulation efficiency (%EE) of the CUR micelles was also carried out. CUR‐loaded PF127 micelles with small particle sizes (around 15 nm, PDI = 0.19 ± 0.13) and %EE around 10% were observed at high centrifugation speed. Also, increasing the copolymer‐to‐drug ratio resulted in higher %EE (&gt;90%). Additionally, the CUR‐loaded PF127 micelles were easy to reconstitute in water by manual shaking after lyophilization. These results suggest that PF127 micelles are a promising carrier for delivery of CUR to the eye. Future work will study in more detail about the physicochemical properties and the ability of these micelles to transport CUR across ocular barriers in ex vivo and in vivo trials.

Isolation of Cellulosic Nano Fibers from Delonix Regia Fruits by Mechano-chemical Route

Lignocellulosic fibres have acquired prominence over past few years among researchers due to its prevalence in nature, biodegradability and high specific strength, among other factors. In this paper, mechanochemical route is used to obtain cellulosic nano fibres (CNF) from raw Delonix Regia fruits. CNFs are extracted with chemical treatments including alkali treatment, bleaching, acid hydrolysis and mechanical treatment including ball milling, centrifugation and high speed homogenization. Characterization of the material produced is carried out using X-Ray Diffraction (XRD), Thermo-Gravimetric Analysis (TGA), Fourier Transform Infrared Spectroscopy (FTIR) and Nano Particle Size Analyzer (NPA). XRD results showed that the crystallinity index (CrI) improved from 52.24% to 73.59% for extracted cellulose whereas it got reduced slightly to 68.31% for CNF samples and the crystallite size extracted cellulose and CNF was estimated to be 6.9 nm and 5.1 nm respectively. TGA results proved that the extracted cellulose and CNF are thermally stable compared to raw DRFF samples. First degradation in TGA started near 70-80C which represents moisture loss. Second degradation temperature improved for cellulose and CNF from 173.6C for DRFF to 223.4C and 246.1C which showed improvement in thermal stability. Major weight loss in second degradation shifted from 298.3C to 346.8C and 348.5C for cellulose and CNF respectively. Ash content was slightly decreased for CNF and cellulose samples compared to DRFF. Typical characteristic IR bands of cellulose were observed at 3400 cm-1, 2800-2900 cm-1, 1643.6 cm-1,1447 cm-1, 1084.6 cm-1 and 673.8 cm-1 of O-H stretching vibrations, C-H stretching, O-H bending, CH2 bending of pyranose ring, C-O-C pyranose ring vibration and O-H out-of-plane bending respectively in extracted cellulose and CNF samples. Improvement in cellulose content was observed from FTIR results and found suitable for application in composite materials. The average particle size of CNF measured by NPA was 247.3 nm proving the abundance of material at nanoscale. The average zeta potential of CNF was estimated at -50 mV with negative sign representing hydroxyl group. From the results of zeta potential measurement, material was found to be electro-statically stable and can be utilized for nanocomposite fabrication.

Optimization of centrifugal casting process parameters by Taguchi method to reduce shrinkage porosity ratio of K417 superalloy

The process parameters were optimized by simulation and verification experiments using orthogonal experimental design and the Taguchi method to lessen the tendency of significant shrinkage porosity in the centrifugal cast ring parts of K417 nickel-based superalloy. Advanced Porosity Model (APM) in ProCAST was used to predict the shrinkage porosity of centrifugal castings, and the effects of centrifugal speed, pouring speed, pouring temperature and preheating temperature of the mold were investigated on the shrinkage porosity ratio of the castings. According to the results, the parameter that has the greatest influence on the shrinkage porosity ratio of centrifugal casting is the centrifuge speed, followed by the preheating temperature of the mold, and the pouring temperature and pouring speed have relatively small effects on it. The optimized parameters were proposed as follows: centrifugal speed of 500 r/min, pouring speed of 225 mm/s, pouring temperature of 1400[Formula: see text], and mold preheating temperature of 50[Formula: see text], which could effectively reduce the formation of shrinkage porosity of the K417 centrifugal casting rings.

Variation in Centrifugal Speed and Resultant Changes in Microstructure and Properties of ZrO2-Toughened Al2O3 Particle-Reinforced Iron Matrix Composites

Variation in Centrifugal Speed and Resultant Changes in Microstructure and Properties of ZrO2-Toughened Al2O3 Particle-Reinforced Iron Matrix Composites

Electrochemical and sensing properties of 2D-MoS2 nanosheets produced via liquid cascade centrifugation

In this paper, layered molybdenum disulfide (2D-MoS2) nanosheets of different size and number of layers were prepared via liquid cascade centrifugation (LCC) and their microstructural, morphological and electrochemical characteristics for the detection of dopamine (DA) and riboflavin (RF) were investigated. The microstructural and morphological characteristics of 2D-MoS2 nanosheets obtained at different centrifugation rates were evaluated by UV-Vis, Raman spectroscopy, and SEM analysis. The 2D-MoS2 nanosheets obtained showed a monotonic decrease of average lateral sheet size <L> and number of layers <N> with the increase of the centrifugation speed. Screen printed carbon electrodes (SPCE) were modified by drop casting on their surfaces 2D-MoS2 nanosheets from aqueous dispersions. The electrochemical properties of the modified 2D-MoS2/SPCE were assessed by electrical impedance spectroscopy and cyclic voltammetry in [Fe (CN)6]3−/4− redox probe. DA and RF were tested to investigate the role of <N> and <L> of MoS2 nanosheets in the electrochemical sensing of these two biomolecules. Results have shown no significant variations in the electrochemical parameters for DA sensing using the different modified electrodes. In contrast, the electro-oxidation of RF was found to be largely promoted on the 2D-MoS2-based electrode with smaller lateral size and minor number of layers, demonstrating the feasibility of tuning the electrochemical properties of 2D-MoS2 nanosheets by modifying their geometric features. The study proposes LCC as an alternative promising technique for preparing nanomaterials with enhanced performances for developing electrochemical sensors for biomolecules.

Technical Surfactant Effect on Precipitation Method of Glutinous Rice Flour Filler Size Reduction

Most of the materials used in dentistry contain fillers as part of their composition to modify their chemical and mechanical properties. Filler size reduction needs economic and environmentally friendly materials to control the viscosity on a simple precipitation method using surfactant. Technical surfactants with more affordable prices also could develop filler size reduction. The study aims to analyse different technical surfactant effects on the glutinous rice flour precipitation method of filler size reduction used as an organic filler in dental applications. Precipitation technique is used by preparing the mixture by adding glutinous rice solution of bi-distilled water or sodium hydroxide with different 4% technical surfactant (Span 80 and Tween 80) dropwise to absolute ethanol. Samples were centrifuged at 3000 and 6000 rpm, then washed and characterized using Scanning Electron Microscope (SEM), Fourier Transform Infra-Red (FTIR), X-ray Diffraction (XRD), and Dynamic Light Scattering (DLS). As the result of this study, a size reduction of 714.8 nm with spherical and rod-like shapes could be generated from glutinous rice flour with the addition of Span 80 technical surfactant in 3000 rpm centrifugation speed for organic filler materials using sodium hydroxide solvent. This study suggested an opportunity for the country’s available source improvement in determining dental materials viscosity and its reinforcement.

High false discovery rate of the Architect anti-HCV screening test in blood donors in Uganda and evaluation of an algorithm for confirmatory testing.

Adequate supplies of donor blood remain a major challenge in sub-Saharan Africa. This is exacerbated by a lack of confirmatory testing for transfusion-transmitted infections by blood transfusion services (BTS), leading to significant blood disposal owing to putatively high seroprevalence rates amongst Ugandan blood donors. We aimed to ascertain the false discovery rate of the Architect anti-hepatitis C virus (HCV) screening assay and categorize screen-reactive samples into three groups: presumed false positive, active and past infection, and develop an algorithm for confirmatory testing. A total of 470 screen-reactive HCV blood donations were retested using the Architect anti-HCV assay, an alternative antibody test (SD Biosensor) and a core antigen (cAg) test. signal-to cut-off (S/CO) ratios and pre-analytical factors (centrifugation speed, haemolysis check, time between collection and testing) were recorded. Based on the S/CO ratio evaluation, we propose a testing algorithm to guide supplemental tests. The false discovery rate of the Architect anti-HCV assay was 0.84 as 395/470 (84%) screen-reactive samples had no evidence of HCV infection (SD Biosensor and cAg negative) (presumed false positive), 38/470 (8.1%) were antigenaemic, and 32/470 (6.8%) had evidence of past infection. The median S/CO ratios of the presumed false-positive and active infection samples were 1.8 and 17.3, respectively. The positive predictive value of HCV positivity in samples with ratios above 12 was 91.8%. On retesting, 104/470 (22.1%) samples became negative. The Architect anti-HCV assay has a very high false discovery rate in Ugandan BTSs, leading to excessive blood disposal. Pre-analytical factors likely contribute to this. An introduction of confirmatory testing using an algorithm based on S/CO ratio evaluation could limit unnecessary blood wastage and donor deferral.

Enhancing Optical Performance for White Light-Emitting Diodes Using Quantum- Dots/Boron Nitride Hybrid Reflective Structure

Quantum dots (QDs) are usually used with phosphor to obtain white light-emitting diodes (WLEDs) due to their excellent lighting characteristics. For lighting effect improvement, the QDs layer and the phosphor layer are supposed to be separated to reduce reabsorption loss and enhance QDs stability. Based on the separated structure, a QDs/boron nitride hybrid reflective (QBHR) structure is proposed to enhance the optical performance of WLEDs in this study. QDs were electrostatically bonded on the boron nitride (BN) pellets and their composite particles were subsequently mixed with silicone for LED encapsulation by a spinning-coated process. It could be easily managed the performance of QBHR structure by controlling the composite particles’ concentration or centrifugal speed. The diffuse reflectance and photoluminescence (PL) intensity of QBHR structure with various thicknesses were analyzed. While applying with remote phosphor layer for WLEDs, the luminous efficacy of devices with QBHR structure increases with phosphor concentration, which are larger than that of QD/polydimethylsiloxane (QP) structures. It may be caused by the light extraction ability of the scattering BN particles and high color conversion efficiency (CCE) of QBHR structure. Finally, under the same correlated color temperature (CCT) of 3640 K, the luminous efficacy and CCE enhance by 14.2% and 19.5% using the QBHR structure, respectively. In addition, the QBHR structure shows better stability than the QP structure after aging for 387.75 h. In brief, the QBHR structure shows an effective and practical packaging method to produce high-performance WLEDs.

Experimental and numerical studies on the influence of centrifugal casting parameters on the solidification structure of Al-Cu alloy

A horizontal centrifugal casting experiment was designed to determine the change in the solidification structure of Al-Cu alloy casting and the underlying variation in temperature, then obtained interface heat transfer coefficient by inverse calculation. The continuous simulation of metal melt filling from the gate to copper mold cavity is realized. The influence of centrifugal speed, pouring temperature, and mold preheating temperature on the solidified structure was analyzed. Simulation results showed that increasing the centrifugal speed mainly enhance the solidification rate of the molten metal and then refined the solidified structure of the Al-Cu alloy. Increasing the pouring temperature and mold preheating temperatures coarsen the grain size of the casting, but the range of change is small.

The Effects of Lengths of Flavin Surfactant N-10-Alkyl Side Chains on Promoting Dispersion of a High-Purity and Diameter-Selective Single-Walled Nanotube.

Flavin with defined helical self-assembly helps to understand chemical designs for obtaining high-purity semiconducting (s)-single-walled carbon nanotubes (SWNT) in a diameter (dt)-selective manner for high-end applications. In this study, flavins containing 8, 12, 16, and 20 n-alkyl chains were synthesized, and their single/tandem effects on dt-selective s-SWNT dispersibility were investigated at isomolarity. Flavins with n-dodecyl and longer chain lengths (FC12, FC16, and FC20) act as good surfactants for stable SWNT dispersions whereas n-octyl flavin (FC8) exhibits poor dispersibility owing to the lack of SWNT buoyancy. When used with small-dt SWNT, FC8 displays chirality-selective SWNT dispersion. This behavior, along with various flavin helical motifs, prompts the development of criteria for ‘side chain length (lS)’ required for stable and dt-selective SWNT dispersion, which also explains lS-dependent dt-enrichment behavior. Moreover, SWNT dispersions with flavins with dodecyl and longer lS exhibit increased metallic (m)-SWNT, background absorption-contributing carbonaceous impurities (CIs) and preferential selectivity of s-SWNT with slightly larger dt. The increased CIs that affect the SWNT quantum yield were attributed to a solubility parameter. Furthermore, the effects of flavin lS, sonication bath temperature, centrifugal speed, and surfactant concentration on SWNT purity and s-/m-SWNT ratio were investigated. A tandem FC8/FC12 provides fine-tuning of dt-selective SWNT dispersion, wherein the FC8 ratio governs the tendency towards smaller dt. Kinetic and thermodynamic assemblies of tandem flavins result in different sorting behaviors in which wide dt-tunability was demonstrated using kinetic assembly. This study highlights the importance of appropriate side chain length and other extrinsic parameters to obtain dt-selective or high-purity s-SWNT.

Comparison of PSA to Moringa Oleifera Seed Protein as Sorbent in QuEChERS: A Response Surface Methodology Optimization for Extraction of Some Endocrine Disrupting Chemicals in Food

This work aimed at optimizing the QuEChERS method with PSA and then comparing it with Moringa Oleifera seed protein as a clean-up sorbent for the extraction of endocrine-disrupting chemicals. The response surface methodology approach was used in the optimization. A design of experiment (DoE) was used to investigate the effect of the sample mass (0.5–3 g), centrifuge speed (3400–4000 rpm) and time (5–20 min), the mass of N a C l and MgSO 4 1 − 3 g , and solvent extraction volume (5–10 mL). The analysis was done using GC-ECD and GC × GC TOFMS. The PSA method which was later replaced with Moringa Oleifera seed protein presented optimal values of 3 g of sample, 150 mg PSA, 4000 rpm for 6 min centrifuge conditions, including 2 g NaCl and 2 g MgSO 4 extracted in 10 mL methanol, respectively. Moringa Oleifera seed protein gave better selectivity, and the detection limits ranged between 0.16 and 1.77 μ g kg − 1 with RSD values ≤ 13.32 % , respectively. Moreover, recoveries were between 76.2 ± 0.85% and 105.2 ± 2.24%. Application of the developed method in food samples detected some EDCs. This study has shown that Moringa Oleifera seed protein is a promising alternative to PSA in the clean-up of food-related samples using the QuEChERS approach.

The non-Newtonian behavior of detergents during concentration is increased by macromolecules, in trans, and results in their over-concentration

Concentration of pure membrane proteins in detergent solution results in detergent concentration, albeit in unknown amounts. This phenomenon is observed in every lab working on membrane proteins, but has seldom been investigated. In this study, we explored the behavior of detergents mixed with membrane proteins during the step of sample concentration using centrifugal devices. We show that detergent over-concentrate with the presence of polymers, typically membrane or soluble proteins but also polysaccharides. The over-concentration of detergents depends on centrifugal force applied to the device. With the use of a specific dye, we observed the formation of a mesh on the concentrator device. Importantly, reducing the centrifugal speed allows to reduce the concentration of detergents when mixed to macromolecules, as tested with 3 different membrane proteins. All together, these results highlight the non-Newtonian behavior of detergents and provides a solid framework to investigators to improve drastically biochemical and structural studies of membrane proteins.

Effects of different specimen pretreatment methods on the measurement of thyrotropin stimulating hormone in serum.

The pre-analysis processing method of serum samples plays a very important role in the assurance of the quality of the entire test and the accuracy of the results. This study illustrates the importance of pretreatment methods of serum samples for the test results by comparing the effects of different pretreatment methods on the measurement of thyroid stimulating hormone (TSH) concentration in serum. In this study, the concentrations of TSH of 37 patients' serum, which were treated in six different ways including the reverse mixing times after blood collection, clotting time and conditions, centrifugal speed and time, were detected on Automatic Chemiluminescence Immunoassay Analyzer, and a comparative analysis of the different results was performed. For serum samples containing coagulants, the test results were significantly affected if the samples were not reversed mixing after collection. The abnormal results would be obtained with insufficient coagulation time, low reaction temperature, low centrifuge speed and insufficient centrifugation time. The pre-analysis processing of serum samples is the beginning of the entire inspection process. The quality of the entire inspection will not be guaranteed if the pre-analysis processing method is irregular. Therefore, clinical laboratories should pay more attention to the pretreatment process of samples to ensure the quality of the entire inspection process.

Second-generation Liquid Platelet Concentrates: A Literature Review.

Liquid or injectable platelet-rich fibrin (PRF) is a second-generation platelet concentrate that is completely autologous and free of external additives, like bovine thrombin and calcium chloride. Additionally, it is the only one to be obtained in a liquid form among the second-generation platelet concentrates. This allows for wide applications such as maximizing injections or mixing with biomaterials such as bone grafts or antibiotics. Since it was first introduced in 2015, several modifications of the original protocol have been proposed which aim at maximizing its biological and mechanical properties. This includes changes in centrifugation speed, time, and so on. The aim of this review is to summarize the various modifications of the injectable/liquid formation of PRF as well as to discuss the potential applications and future research direction.

A rapid method for isolation of bacterial extracellular vesicles from culture media using epsilon-poly-L-lysine that enables immunological function research.

Both Gram-negative and Gram-positive bacteria can release vesicle-like structures referred to as bacterial extracellular vesicles (BEVs), which contain various bioactive compounds. BEVs play important roles in the microbial community interactions and host-microbe interactions. Markedly, BEVs can be delivered to host cells, thus modulating the development and function of the innate immune system. To clarify the compositions and biological functions of BEVs, we need to collect these vesicles with high purity and bioactivity. Here we propose an isolation strategy based on a broad-spectrum antimicrobial epsilon-poly-L-lysine (ϵ-PL) to precipitate BEVs at a relatively low centrifugal speed (10,000 × g). Compared to the standard ultracentrifugation strategy, our method can enrich BEVs from large volumes of media inexpensively and rapidly. The precipitated BEVs can be recovered by adjusting the pH and ionic strength of the media, followed by an ultrafiltration step to remove ϵ-PL and achieve buffer exchange. The morphology, size, and protein composition of the ϵ-PL-precipitated BEVs are comparable to those purified by ultracentrifugation. Moreover, ϵ-PL-precipitated BEVs retained the biological activity as observed by confocal microscopy studies. And THP-1 cells stimulated with these BEVs undergo marked reprogramming of their transcriptome. KEGG analysis of the differentially expressed genes showed that the signal pathways of cellular inflammatory response were significantly activated. Taken together, we provide a new method to rapidly enrich BEVs with high purity and bioactivity, which has the potential to be applied to BEVs-related immune response studies.

Concentration of lycopene from watermelon juice using cross-flow filtration assisted with diafiltration

Watermelon is a well-known and high-consumed fruit in Vietnam, considered a rich source of bioactive compounds, especially lycopene. The cross-flow filtration coupled with diafiltration was processed to obtain the concentrated lycopene. The microfiltration process was conducted at a temperature of 50-60oC by using a ceramic α-Al2O3 membrane with a pore size of 0.08 µm. Permeate was removed while the collected retentate was fractionated into two parts for further experiments. The first part of the retentate was directly centrifuged. The effect of centrifugation time (2, 5, and 8 mins) and speed (1000, 3000, and 5000 rpm) on lycopene content were evaluated. The other part of the retentate was purified by the diafiltration process. The ratio of retentate: water was varied from 1:0.5 to 1:2 (w/w) to determine its influence on the lycopene content. The results showed that the suitable transmembrane pressure was selected at 5 bars. The membrane fouling phenomena was also observed by a rapid decrease in permeate flux in 40 min, followed by a gradual reduction. The suitable condition of centrifugation was processed at 3000 rpm in 5 mins. The centrifuged retentate in 60 mins of microfiltration contained a lycopene content of 146.63±2.76 µg/mL. The retentate: water ratio of 1:0.5 was noted as an effective ratio to purify the lycopene content, achieving 273.42±4.56 µg/mL. The physicochemical properties of the raw watermelon juice and retentate indicated a positive result from the retentate with enhanced total solid content, lycopene concentration as well as purity.