Abstract
Nano-chitosan can be produced from blood clams (Anadara granosa) because they contain 14-35% of chitin. The production of nano-chitosan can be conducted by a bottom-up process using sodium tripolyphosphate (Na-TPP). The aims of this study are to produce nano-chitosan from blood clamshell and to study the factor affecting the particle size of nano-chitosan such as the ratio of chitosan to Na-TPP solution (v/v) and rotation speed of the centrifuge. The research shows that The chitin content on blood clamshell is 25.42%. The yield of chitosan from chitin is about 80.92%. The degree of deacetylation of chitin from blood clamshell reaches 63.18%. The effect of the ratio of chitosan to Na-TPP solution (R) and the rotation speed of centrifuge (N) on the particle size of nano-chitosan can be expressed by equation dp = 0.12 (R)0.714 (N)0.99.
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