In this paper, we describe an Operational Modal Analysis (OMA) of the Bridge over the Tocantins River, a composite road-rail bridge located in the city of Maraba, state of Para, northern Brazil. The bridge is part of the Carajas Railway, which is used by VALE Company to transport iron ore from “Serra dos Carajas”, the largest mineral reserve of the planet. The bridge has a length of 2310m, being the second longest road-rail bridge in Brazil. The study focused on the bridge central span (with 77 m of length) and two adjacent spans (with 44 m of length). We employed Piezo-electric accelerometers to measure the dynamic response of the bridge under ambient excitation, which consisted of the passage of loaded and unloaded trains, road traffic, wind and river current. For modal identification, to avoid the influence of the train mass over the bridge system, we only considered acceleration signals of road traffic and signals corresponding to intervals after the passage of loaded trains, i.e., the study disregarded the intervals during which the trains were over the monitored spans. We obtained modal parameters through two identification methods: one in the time domain and other in the frequency domain. We also performed a comparative analysis between the experimental modal parameters, and the numerical results obtained with a finite element model provided by a third-part company.