AIM : Comparative evaluation of apical sealing ability of three different endodontic sealers : An in vitro study Materials and Methods:- The study was conducted on 60 samples on single rooted teeth using three different sealers MTA fillapex, Apexit Plus, Bio C sealer. On the prepared samples access cavities and biomechanical preparation was performed using endodontic rotary system. The teeth were randomly divided into three groups of 15 each with control group having empty canal. All root surfaces except the apical 2 mm was covered with two layers of nail varnish and sticky wax and all samples were immersed in an aqueous solution of 2% methylene blue dye for 72 hours. Roots were longitudinally split using a diamond disc . STATISTICAL ANALYSIS : The data was statistically analysed with One way ANOVA test and post hoc tukey test to compare the apical sealing ability between the experimental groups RESULTS : In our study , least sealing ability was observed Apexit plus followed by MTA Fillapex and Bio C sealer.CONCLUSION : Bio C sealer showed considerably good results compared to MTA Fillapex and Bio C sealer