Cellulosic Waste Research Articles

Characterization of Mycelium Biocomposites under Simulated Weathering Conditions.

Expanded polystyrene (EPS) remains a popular packaging material despite environmental concerns such as pollution, difficulty to recycle, and toxicity to wildlife. The goal of this study is to evaluate the potential of an ecofriendly alternative to traditional EPS composed of a mycelium biocomposite grown from agricultural waste. In this material, the mycelium spores are incorporated into cellulosic waste, resulting in a structurally sound biocomposite completely enveloped by mycelium fibers. One of the main criteria for shipping applications is the ability of a material to withstand extreme weather conditions. Accordingly, this study focused on evaluating a commercially available mycelium material before and after exposure to various weathering conditions, including high and low temperatures at different humidity levels. Fourier transform infrared spectroscopy (FTIR) was performed to examine any transformations in the mycelium structure and composition, whereas scanning electron microscopy (SEM) was used to reveal any changes in the morphology. Similarly, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyses were conducted to evaluate the thermal behavior, whereas mechanical properties were measured by using shore hardness and Izod Impact testing. Although some irreversible changes were observed due to the exposure to high temperatures, the material exhibited good thermal stability and impact resistance. FTIR analysis demonstrated small changes in the biocomposite structure and protein rearrangement as a result of weathering, whereas SEM revealed some cracking in the cellulose substrate. A combination of low temperatures and humidity resulted in significant moisture absorption, as indicated by TGA and DSC. This in turn decreased the hardness of the fibers by nearly 2-fold; however, the impact strength of the entire biocomposite remained unchanged. Overall, these results provide important insight into the structure-property relationships of mycelium-based materials.

Microorganisms of solid waste as an opportunity for waste disposal and increasing environmental sustainability in the south of Kazakhstan

The study is devoted to the problem of processing the organic waste that is generated as a result of paper, textiles and other industries production as well as food waste. The growth of economic activity in Kazakhstan has resulted in a significant challenge with regard to industrial waste management. The accumulation of waste on the territory of the country has reached 31.72 billion tonnes, comprising approximately 2.5 billion tonnes of hazardous waste, 50 million tonnes of phosphorus-containing waste, over 2.5 million tonnes of lead-zinc waste and more than 120 million tonnes of solid domestic waste. The study object was the Shymkent-Kokys polygons. According to the research carried out, it was determined that the titer of microorganisms of the studied groups is 1–10 CFU/g in the soils selected around the garbage in the area of the Shymkent landfill. The titer of microorganisms in the soil horizons was high at a depth of 20–30 cm and the titer were 109 cells/mL. The structure of the soil microbiome obtained around the Shymkent Waste Landfill consists of actinomycetes, micromycetes, heterotrophic bacteria, nitrifying, nitrogen-fixing bacteria, enterobacteria, as well as algae and protozoa. It was found that strains KPA1, KPA2 Pseudomonas sp. strains KPA3, KPA4, KPA5 Bacillus sp. isolated from the soils of the Shymkent-Kokys landfill are able to recycle domestic waste with a high content of cellulose and organic substances up to 95%–97%. The findings can be used to develop more effective organic cellulosic waste management strategies and improve the environmental sustainability of various industries.

Eco-sustainable and flexible SERS platform based on waste cellulose decorated by Ag nanoparticles

This paper presents an innovative and environmentally friendly technology for the fabrication of low-cost SERS (Surface-Enhanced Raman Spectroscopy) sensors based on flexible substrates made of cellulose fibers reclaimed from waste. The substrates are decorated with nanostructured silver (Ag) thin films produced by pulsed laser deposition (PLD). In this process, the deposition conditions (laser fluence, gas pressure, target-substrate distance, deposition time, etc.) were optimized to enhance the SERS response. Different types of paper with different textures were tested, as it was also observed that the paper roughness significantly influences SERS efficiency. The samples were characterized using UV–Vis absorption spectroscopy, SEM microscopy, and surface profilometry to evaluate both the paper and the deposited films' morphologies. The SERS activity was assessed by detecting Rhodamine 6G in aqueous solutions drop-casted on the sensors, with concentrations ranging from 10−2 M to 10−10 M. Measurements were carried out using a handheld instrument equipped with dual excitation laser lines centered at 785 nm and 833 nm. The observed lower detection limit of 10−10 M was achieved across all paper types tested. These results demonstrate the potential of integrating smart, eco-friendly materials in the fabrication of chemical sensors for sustainable advancement in environmental monitoring and safety. The materials not only exhibit excellent sensing capabilities but also minimize ecological footprints through renewable sourcing and eco-friendly production processes. While the deposition protocol is well-established for other substrates, this study marks the first exploration of its use on biomass-derived substrates.

A readily accessible quaternized cellulose filter paper with high permeability for IgG separation

Anion-exchange chromatography (AEC) is recognized as a highly effective approach for the purification of immunoglobulin G (IgG). This study introduces an innovative strategy that employs waste cellulose filter paper in the production of AEC media. A quaternized cellulose fiber membrane (CFM-QCS) was successfully fabricated that cellulose fibers were as a structural framework, glutaraldehyde (GA) as a crosslinking agent, and quaternary chitosan (QCS) as a modifying agent. Morphological and chemical characterization revealed that GA and QCS were uniformly crosslinked on the surface of the cellulose fibers, resulting in excellent mechanical properties in both dry and wet states. Benefiting from its 3D network scaffold structure, CFM-QCS demonstrated a high adsorption capacity for bovine serum albumin (BSA), with static and dynamic adsorption capacities of 605.15 mg/g and 88.63 mg/ml, respectively. After treated with extreme conditions and 10 cyclic adsorption and elution, the adsorption capacity of CFM-QCS remains almost unchanged, highlighting its excellent stability. Additionally, a CFM-QCS packed chromatography column exhibited high flux of 10.38 L/h at 0.1 MPa, which can efficiently separate IgG from a mixed solution in the presence of BSA and IgG by gravity-driven. This work presents a straightforward approach for preparing high-performance ion-exchange chromatography membranes for IgG separation.

Valorization of Cellulosic Waste from Artichoke for Incorporation into Biodegradable Polylactic Acid Matrices.

This study presents the development of ecological compounds using polylactic acid (PLA) and artichoke flour with the aim of obtaining materials with properties like commercial PLA. PLA biocomposites with different concentrations of green artichoke (HV) and boiled artichoke (HH) (1, 3, 5, 7, 10 and 20% by weight) were manufactured through an extrusion and injection process. Structural, mechanical, physical and color tests were carried out to analyze the effect of lignocellulosic particles on the biopolymeric matrix. The Shore D hardness, elongation at break and heat deflection temperature (HDT) of the PLA/HV and PLA/HH samples showed similar values to pure PLA, indicating that high concentrations of both fillers did not severely compromise these properties. However, reductions in the tensile strength, impact strength and Young's modulus were observed, and both flours had increased water absorption capacity. FTIR analysis identified the characteristic peaks of the biocomposites and the ratio of the groups regarding the amount of added filler. The SEM revealed low interfacial adhesion between the polymer matrix and the filler. This study represents a significant advance in the valorization and application of circular economy principles to agricultural waste, such as artichoke waste. PLA/HV biocomposites make a substantial contribution to sustainable materials technology, aligning with the goals of the 2030 agenda to reduce environmental impacts and promote sustainable development.

Metabolic pathway analysis of the biodegradation of cellulose by Bacillusvelezensis

A green strategy that using Bacillus velezensis to degrade cellulose in bamboo forest waste was demonstrated. The cultivation conditions of microorganism, identified as Bacillus velezensis by 16S rDNA, were optimized by response surface methodology (RSM). Results showed that with a temperature of 35 °C, a pH of 6 in a Luria-Bertani (LB) medium, and a rotation speed of 140 r/min, the bamboo forest waste powder was added to the purified bacteria at a dose of 1 % (w/v), the cellulose degradation rate reached the highest value of 33.12 %. Besides, the fourier-transform infrared (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and liquid chromatography-mass spectrometry (LC-MS) indicated that cellulose could be effectively degraded, with possible products including cellobiose, uridine diphosphate (UDP)-glucose, D-glucuronic acid, etc. The metabolic pathways involved in cellulose degradation by this strain include the pentose phosphate, starch, sucrose, and glucuronic acid pathways.

Nanofabrication of Biochar from Cellulosic Waste for Bio-Sensing Application of Waste Water Treatment: Process, Challenges and Future Update

Nanofabrication of Biochar from Cellulosic Waste for Bio-Sensing Application of Waste Water Treatment: Process, Challenges and Future Update

Safe and promising bioconversion of rice cellulosic waste into algal biomass

Safe and promising bioconversion of rice cellulosic waste into algal biomass

Novel Recycling, Defibrillation, and Delignification Methods for Isolating α-Cellulose from Different Lignocellulosic Precursors for the Eco-Friendly Fiber Industry.

Alpha-cellulose, a unique, natural, and essential polymer for the fiber industry, was isolated in an ecofriendly manner using eleven novel systems comprising recycling, defibrillation, and delignification of prosenchyma cells (vessels and fibers) of ten lignocellulosic resources. Seven hardwood species were selected, namely Conocorpus erectus, Leucaena leucocephala, Simmondsia chinensis, Azadirachta indica, Moringa perigrina, Calotropis procera, and Ceiba pentandra. Moreover, three recycled cellulosic wastes were chosen due to their high levels of accumulation annually in the fibrous wastes of Saudi Arabia, namely recycled writing papers (RWPs), recycled newspapers (RNPs), and recycled cardboard (RC). Each of the parent samples and the resultant alpha-cellulose was characterized physically, chemically, and anatomically. The properties examined differed significantly among the ten resources studied, and their mean values lies within the cited ranges. Among the seven tree species, L. leucocephala was the best cellulosic precursor due to its higher fiber yield (55.46%) and holocellulose content (70.82%) with the lowest content of Klasson lignin (18.86%). Moreover, RWP was the best α-cellulose precursor, exhibiting the highest holocellulose (87%) and the lowest lignin (2%) content. Despite the high content of ash and other additives accompanied with the three lignocellulosic wastes that were added upon fabrication to enhance their quality (10%, 11%, and 14.52% for RWP, RNP, and RC, respectively), they can be considered as an inexhaustible treasure source for cellulose production due to the ease and efficiency of discarding their ash minerals using the novel CaCO3-elimination process along with the other innovative techniques. Besides its main role for adjusting the pH of the delignification process, citric acid serves as an effective and environmentally friendly additive enhancing lignin breakdown while preserving cellulose integrity. Comparing the thermal behavior of the ten cellulosic resources, C. procera and C. pentandra exhibited the highest moisture content and void volume as well as having the lowest specific gravity, crystallinity index, and holocellulose content and were found to yield the highest mass loss during their thermal degradation based on thermogravimetric and differential thermal analysis in an inert atmosphere. However, the other resources used were found to yield lower mass losses. The obtained results indicate that using the innovative procedures of recycling, defibrillation, and delignification did not alter or distort either the yield or structure of the isolated α-cellulose. This is a clear indicator of their high efficiency for isolating cellulose from lignocellulosic precursors.

Revealing the sodium storage mechanism of cellulose separator-derived hard carbon anode materials for sodium-ion batteries

Hard carbon (HC) shows significant promise as a material for sodium-ion batteries (SIB). Cellulose-derived carbon is considered one of the most promising high-performance anode materials for sodium-ion batteries. In this study, waste cellulose separator-derived HC anode materials were rationally developed using the pre-oxidation-carbonization pyrolysis method. The as-prepared HC possessed advantageous characteristics for Na+ storage, including a unique fibrous structure, a reasonable space layer, and a small specific surface area (SSA). The optimal sample demonstrated a substantial reversible capacity of 361.29 mAh/g at 0.1C, an impressive high-rate performance with a reversible capacity of 272.93 mAh/g at 10C, and an initial coulombic efficiency of 84.85 %. Following 500 cycles at 1C, the capacity retained 96.38 % of its initial value. The sodium storage process was identified as the “adsorption-insertion-pore filling mechanism” through ex-situ XRD and in-situ Raman experiments. Moreover, the as-prepared HCs demonstrated better rate and cycle capability when evaluated as anodes in full-cell sodium-ion batteries (SIBs). This research provides valuable insights into the rational design of high-performance HC anodes for SIBs and other applications.

Chemical recycling of waste cellulose denim fabric and re-dyeing process

Chemical recycling of waste cellulose denim fabric and re-dyeing process

Alkali pretreatment-assisted catalytic pyrolysis of waste cellulose acetate for selective preparation of levoglucosenone

With the increasing demand for cellulose acetate (CA) in industrial and daily applications, the disposal of its waste has emerged as an environmental concern. In this study, a novel and sustainable method for value-added utilization of waste CA was proposed, which involved NaOH pretreatment and subsequent catalytic pyrolysis using phosphoric acid-activated carbon (PAC) to prepare levoglucosone (LGO). The effects of pretreatment temperatures and NaOH concentrations on the physicochemical characteristics of CA were thoroughly investigated. Furthermore, the pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) experiments were conducted to methodically assess the impacts of both pretreatment and pyrolysis conditions on the production of LGO. The results indicated that NaOH pretreatment could effectively hydrolyze the ester bonds in CA, leading to the removal of acetyl groups and a decrease in thermal stability. At a pretreatment temperature of 40 °C, a NaOH concentration of 0.5 mol/L, a catalyst/sample ratio of 1/3, and a pyrolysis temperature of 400 °C, the LGO yield could reach 11.52 wt%, much higher than that obtained from catalytic pyrolysis of CA (2.70 wt%). This study provides an efficient method for treating waste CA and demonstrates its potential for conversion into high-value chemicals, laying a solid foundation for the green recycling of waste plastics.

Development of a method for producing carbon sorbents by thermochemical pyrolysis of liquid cellulose waste

Development of a method for producing carbon sorbents by thermochemical pyrolysis of liquid cellulose waste

MXene assisted simple recycle of waste cellulose fiber with alginate fiber into fireproof and electromagnetic interference shielding composite

Textile wastes rapidly grow into a challenge when a significant portion of them are processed by landfilling or incineration, leading to hazardous solid and volatile pollutants. This work investigated the utilization of wasted cellulose fiber by recycling it with calcium alginate fiber into a fireproof composite filler as a circular economy strategy, followed by the modification with MXene dispersion to further enhance its fire resistance and offer it the electromagnetic interference shielding ability. A comprehensive investigation elucidated the flame retardant mechanism of the composite felt via studying its combustion behavior, the microstructural morphology of residue char, and the gasous compounds produced. The results indicated that this composite felt generated a large number of nonflammable gas species and fibrous residue char, serving as a natural barrier to impede the fuel supply and suppress heat diffusion, thereby endowing the composite felt with an outstanding fire retardant performance and reduced carbon footprint. Compared to other textile waste recycling processes, the opening-carding-needling punch technique employed in this study was more energy-efficient and environmentally friendly. The recycling of waste cellulose fiber into functional fireproof composites not only extended the practical applications of waste resources but also mitigated the negative environmental impact of textile disposal.

Lipid production from corn straw by cellobiohydrolase and delta-6 desaturase engineered Mucor circinelloides strains under solid state fermentation

Previously, we constructed engineered M. circinelloides strains that can not only utilize cellulose, but also increase the yield of γ-linolenic acid (GLA). In the present study, an in-depth analysis of lipid accumulation by engineered M. circinelloides strains using corn straw was to be explored. When a two-stage temperature control strategy was adopted with adding 1.5% cellulase and 15% inoculum, the engineered strains led to increases in the lipid yield (up to 1.56 g per 100 g dry medium) and GLA yield (up to 274 mg per 100 g dry medium) of 1.8- and 2.3-fold, respectively, compared with the control strain. This study proved the engineered M. circinelloides strains, especially for Mc-C2PD6, possess advantages in using corn straw to produce GLA. This work provided a reference for transformation from agricultural cellulosic waste to functional lipid in one step, which might play a positive role in promoting the sustainable development of biological industry.

Possibilities of producing lightweight aggregates from silica-clay raw material and cellulosic waste

The subject of the work is the characterization of lightweight aggregates intended for construction using the example of silica-clay raw material from south-eastern Poland (Dylągówka-Zapady deposit). The primary objective of the research is to assess the possibility of managing the accumulated organic waste, including cellulose pulp. This storage is problematic for processing plants and local government bodies and incurs high costs annually. Therefore, based on the patent (Kukułka et al. Method of producing raw meal, dried clay granules, and fired clay granules and the production line of raw meal, dried clay granules, and baked clay granules. Patent RP no P.429284.), an attempt was made to confirm the assumptions of the technology of fired clay granules, which can be used for zootechnical, agricultural, and construction purposes. The presented work assessed the physicochemical transformations in the clay-silica raw material after adding a significant amount of cellulose waste (10%, 20%, and 30%) in the temperature range of 25–1300 °C were assessed. Thermal methods such as thermodilatometry, Hot-Stage Microscopy with image analysis (IA-HSM) and thermal DSC/TG analysis with gas analysis were used for this purpose. Based on the first method, a temperature range was established in which sintering occurs without an apparent effect of densification. Therefore, the range of 850–950 °C was used to obtain fired granules, which were tested as a material for lightweight aggregates.

Synthesis and characterization of biocompatible hydrogel from textile–garment waste for personal hygiene products

The objective of the present study is to synthesise cellulose-based hydrogel from cellulosic waste that is produced in the garment and textile industries. Carboxymethyl cellulose was synthesized via carboxymethylation subsequent to the separation of cellulose. The progress of reaction was monitored by measuring the degree of carboxymethyl cellulose substitution. Carboxymethyl cellulose was graft co-polymerized with acrylic acid and acrylamide in the presence of N,N-methylene-bis-acrylamide to develop a superabsorbent hydrogel. The generated hydrogel was put through several mechanical and physical tests, as well as FTIR, XRD, TGA and SEM investigations, to evaluate its real formation and characteristics. The prepared hydrogel demonstrated favorable swelling properties, as well as excellent adsorption of ions and dyestuffs. The maximum water absorption capability of the superabsorbent material obtained from textile and garment waste was 182 g/g. Furthermore, the prepared hydrogel exhibits exceptional biocompatibility, biodegradability, environmental friendliness, and non-toxicity. These qualities make it an exceptional material for use in personal care items and protective gear.

Upcycling of tetra pack waste cellulose into reducing sugars for bioethanol production using Saccharomyces cerevisiae

Bioethanol production from waste materials offers a promising avenue for sustainable energy and waste management. In this study, fermentable sugars derived from tetra pack waste cellulose were bio-transformed into bioethanol using Saccharomyces cerevisiae. Tetra pack waste (180 g) yielded tetra pack cellulosic pulp (TPCP) of 145 g, after removing the different layers representing 80.56 ± 0.32% of the original weight. Cellulase from Bacillus sp. RL-07, with a cellulolytic potential of 6.98 ± 0.36 U/ml, released 32.72 ± 0.12 mg/ml of reducing sugars, achieving 44.60 ± 0.56% saccharification of TPCP under optimized conditions. Subsequent fermentation of the broth (1 L) with tetra pack cellulosic pulp hydrolysate (TPCPH) (50% v/v), containing 5.12 g of reducing sugars, by S. cerevisiae yielded 1.42 g of bioethanol per g of reducing sugars under optimized conditions, with a volume productivity of 0.24 g/l/h and a purity of 96.42% was confirmed by GC/MS analysis.The results of this study underscore the viability of utilizing tetra pack waste for bioethanol production, offering a sustainable solution for waste management while alleviating energy deficits and reducing environmental pollution. These findings align with objectives aimed at fostering sustainable progress and development.

Biodegradation of Cellulosic Wastes and Deinking of Colored Paper with Isolated Novel Cellulolytic Bacteria

Biofuels are the cheapest source of energy, and the continuous decline of traditional sources of energy with the increasing population leads to looking for alternatives to reduce the consumption of traditional sources of energy. Bioethanol production from lignocellulosic wastes and cellulosic wastes is not a new approach for fuel production but a cheap and accessible way for the production of fuel. Bacillus is one of the major species that can act as a source of diversified enzymes. In this study, it was emphasized on screening and isolation of a novel, characterization, and best catalytic action on both celluloses and proteins in the presence of different carbon and nitrogen sources. It was observed the effective catalytic breakdown of cellulose with the crude enzyme to glucose allowed fur for fermentation with Saccharomyces, ultimately leading to the generation of alcohol. The study aims to isolate the microbes that can produce cellulases and enzymes and could be used for biodegradation to produce ethanol in the reaction. The maximum enzyme activity was achieved at 3.112 UI with optimized pH and temperature, and the maximum conversion of sugars into alcohol was about 70% in the newspaper, cartons, colored paper, and disposable paper cups. An essential observation was the decolorization of the origami craft paper within 24 hours. The study was involved in enhancing the maximum Enzyme activity of cellulases from different cellulosic raw materials. Hence, it was achieved by JCB strain, optimization of pH, temperature, and acids for the biodegradation. The presence of peaks at 3200 and 2900 was a confirmation of ethanol bonds in the biodegradation reaction mixtures.

Upcycling of cellulosic textile waste with bacterial cellulose via Ioncell® technology

Currently the textile industry relies strongly on synthetic fibres and cotton, which contribute to many environmental problems. Man-made cellulosic fibres (MMCF) can offer sustainable alternatives. Herein, the development of Lyocell-type MMCF using bacterial cellulose (BC) as alternative raw material in the Ioncell® spinning process was investigated. BC, known for its high degree of polymerization (DP), crystallinity and strength was successfully dissolved in the ionic liquid (IL) 1,5-diazabicyclo[4.3.0]non-5-enium acetate [DBNH][OAc] to produce solutions with excellent spinnability. BC staple fibres displayed good mechanical properties and crystallinity (CI) and were spun into a yarn which was knitted into garments, demonstrating the potential of BC as suitable cellulose source for textile production. BC is also a valuable additive when recycling waste cellulose textiles (viscose fibres). The high DP and Cl of BC enhanced the spinnability in a viscose/BC blend, consequently improving the mechanical performance of the resulting fibres, as compared to neat viscose fibres.