Cellulose TLC Research Articles

Biosynthese d'un mannolipide a partir de guanosine diphosphate mannose dans les microsomes d'hepatocytes de congre ( Conger vulgaris)

1. 1. Conger liver microsomes catalyse the formation of a glycolipid from GDP-mannose. 2. 2. The purification of this glycolipid by column chromatography on silicic acid and DEAE-cellulose, its analysis by TLC on silica-gel G and its hydrolytic characteristics show that it is a polyprenol-phosphate-sugar. 3. 3. After acid hydrolysis, the sugar moiety is purified by ion-exchange chromatography and analysed on cellulose TLC in two different solvants. The migration data are in agreement with a unique constituent which is mannose.

A double-labeling procedure for sequence analysis of picomole amounts of nonradioactive RNA fragments.

A double-labeling procedure for sequence analysis of nonradioactive polyribonucleotides is detailed, which is based on controlled endonucleolytic degradation of 3'-terminally (3H)-labeled oligonucleotide-(3') dialcohols and 5"-terminal analysis of the partial (3H)-labeled fragments following their separation according to chain length by polyethyleneimine- (PEI-)cellulose TLC and detection by fluorography. Undesired nonradioactive partial digestion products are eliminated by periodate oxidation. The 5'-termini are assayed by enzymic incorporation of (32p)-label into the isolated fragments, enzymic release of (32p)-labeled nucleoside-(5') monophosphates, two-dimensional PEI-cellulose chromatography, and autoradiography. Using this procedure, as little as 0.1 - 0.3 A260 unit of tRNA is needed to sequence all fragments in complete ribonuclease T1 and A digests, whereas radioactive derivative methods previously described by us1-4 required 4 - 6 A260 units.

The synthesis of a radioactive cytokinin with high specific activity

6-benzylaminopurine-[ p- 1H-benzyl] at a specific activity of 10 Ci/mmol was synthesized by reacting p-bromo-6-benzylaminopurine with carrier-free tritium gas in the presence of 10% Pd/C. A radiochemical purity of 97% was obtained by a one-step purification of the tritiated reaction product using cellulose TLC. This simple procedure yields the highly active cytokinin, 6-benzylaminopurine, with tritium at near maximum specific activity in a known, stable position.

The resolution of pyrimidine bases, nucleosides, and nucleotides by thin-layer chromatography

Nucleic acid research frequently necessitates the analytical resolution of nucleic acid derivatives. Thin-layer chromatography (tlc), for its simplicity, short development time, and superior resolving power, is often preferable to other methods (1–3). Although the literature contains a large number of methods that have been devised for the separation of purine and pyrimidine derivatives (4,5) no tlc technique has hitherto been described for the concomitant separation of bases, nucleosides, nucleoside 5′-monophosphates, nucleoside 3′-monophosphates, nucleoside diphosphates, and nucleoside triphosphates. The present communication deals with methods devised for the simultaneous separation of the above-mentioned pyrimidine derivatives. They enable the resolution of either the six uracil derivatives or the six cytosine derivatives, on commercial cellulose tlc sheets. Alternatively, the six pyrimidine derivatives can be separated on cellulose layers 0.75 mm thick. Since formic acid extracts of bacterial cells do not interfere with the separation, these methods can be used for the direct estimation of extracts of biological materials.

Phenolic acids as indicators of pollution with liquid manure. A method for their detection

Detection of a combination of 2 or 3 of the phenolic acids m-hydroxybenzoic acid, m-hydroxyphenylacetic acid and m-hydroxyphenylpropionic acid by a method described indicates whether a water sample contains liquid manure. The method using thin-layer chromatography on cellulose is designed on the basis of results from qualitative and quantitative determinations of the phenolic acids in liquid manure by paper chromatography and gas chromatography respectively. It was developed in order that illegal discharges of liquid manure could be detected. The phenolic acids is extracted with ethyl acetate from a volume of acidified sample equalling 1 mg of oxygen consumed (measured with potassium permanganate). The ethyl acetate is evaporated and the residue dissolved in ethanol. After spotting of a 1 μl, aliquot on a cellulose TLC plate the chromatogram is developed by capillary ascent with the solvent n-propanol: n-butanol: 25° o NH 3:water (4:4:1:1, by vol). The solvent front is allowed to advance 10 cm. This takes about 2·5 h. The air-dried plate is sprayed with a diazotized p-nitroaniline reagent to make the phenolic acids visible. The application of the method has been tested with different sources of waste water. The phenolic acids was detectable in 23 out of 28 samples of stored liquid manure. A degradation study, where liquid manure was diluted 100 times with stream water and then aerated, showed decomposition of the phenolic acids after a few days. However the addition of NaOH to samples prevents decomposition of the phenolic acids.

Identification of N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine and bufotenin by cellulose TLC

Identification of N,N-dimethyltryptamine, 5-methoxy-N,N-dimethyltryptamine and bufotenin by cellulose TLC

A method for the estimation of indolylacryloylglycine in urine

A method consisting of extraction, Sephadex G-10 column chromatography, two-dimensional cellulose TLC and UV spectrophotometry has been developed which allows a specific estimation of down to 2 μg indolylacryloylglycine per 100 mg creatinine.

Chlorophyll degradation products from processed pea purée

In addition to chlorophylls a and b and pheophytins a and b, three chlorophyll degradation products were found in pH-elevated, high temperature-short time processed pea purée after storage for 18 months at room temperature. The pigments, separated and purified by cellulose TLC, are shown to have chemical and spectral properties similar to various alteration products of pheophytins a and b and chlorophyll b. Possible structures of the three pigments are discussed.

Photographic detection and documentation of U.V.-absorbent and fluorescent nucleotides on cellulose TLC plates

Photographic detection and documentation of U.V.-absorbent and fluorescent nucleotides on cellulose TLC plates