Cell Production System Research Articles

Flexible Manufacturing by Application of RFID and Sensors in Robot Cell Manufacturing Systems

Cell production in which only a few human operators manufacture products by themselves is popular in Japan due to its flexibility. However, the decrease in the number of young persons in developed countries will make it difficult to do in the near future. Therefore, cell production using robots is going to be needed. A cell production system using a robot and automatic machines having special functions is developed. The robot transfers parts and tools for the automatic machines. A RFID is attached to each part, and the quick change in the functions of the automatic machines is executed as the kinds of products change. Adaptive tool setup scheduling for the change in operation times of the special function machines is executed and the improvement of product efficiency is analyzed. Many vision sensors are used for the adjustment of the position and orientation of the robot hand and parts, and distributed image processing for the hand and parts make rapid motion adjustment possible.

OS2-4 ロボット制御セル生産システムのリスクアセスメントに基づく安全機器の最適設置と作業者の安全の飛躍的向上について(0S2 制御システムの安全)

OS2-4 ロボット制御セル生産システムのリスクアセスメントに基づく安全機器の最適設置と作業者の安全の飛躍的向上について(0S2 制御システムの安全)

Cell cycle phase dependent productivity of a recombinant Chinese hamster ovary cell line

A Chinese Hamster Ovary cell line, CHO1-15(500), producing recombinant human tissue type plasminogen activator (tPA) via the dihydrofolate reductase (DHFR) amplification system, was studied in batch culture. In this system both DHFR and tPA are under the control of the strong constitutive viral SV40 early promoter. Employing the cumulative viable cell-hour approach, the specific productivity of tPA had maxima in the lag (0.065 pg cell(-1 )h(-1)) and early decline (0.040 pg cell(-1 )h(-1)) population growth phases. The viable population was assigned into four subpopulations (G1, S, G2/M phase, and Apoptotic cells) using flow cytometric analysis. As expected, intracellular DHFR was maximally expressed during the S cell cycle phase. The production of tPA, however, was found to be a direct linear function of the G1 phase, with a subpopulation specific productivity of 0.080 pg c-h(-1). Productivity maxima in the lag and early decline corroborate the flow cytometric data, indicative that this recombinant tPA production occurs primarily in the G1 cell cycle phase, not the S phase. This suggests that endogenous regulatory mechanisms are important controlling influences on the production of recombinant tPA in this ovarian cell line. Productivity from recombinant cell lines cannot be inferred from either the plasmid construct or the host cell alone. Elucidation of the relationship between expression of recombinant protein and the cell cycle phases of the host cell is a major component of the characterization of the animal cell production system. This information facilitates rational process design, including operating mode, modelling and control, and medium formulation.

The shift from belt conveyor line to work-cell based assembly systems to cope with increasing demand variation in Japanese industries

The increasing market turbulence has impelled leading manufacturers to search the development of alternative production systems supposed to enhance their responsiveness to the market. The purpose of this paper is to discuss the turnaround currently observed in some manufacturing industries in Japan, which are migrating to more human-centred production systems. The pattern of production organisation that has emerged from this trend has been called 'cell production system' and should not be confounded with cellular manufacturing system. This paper intends to elucidate the rationality behind the conversion into cell production approach and examines the major features of this production organisation pattern.

Motion and Arrangement Planning for Self-Moving Trays in Human Supporting Production Cell “Attentive Workbench”

We have proposed “Attentive Workbench (AWB),” a new cell production system in which an intelligent system supports human workers. Recognizing worker's condition and intention, the system supports workers from both physical and information aspects. This paper deals with physical assembly support using self-moving parts trays. The system delivers necessary assembly parts to workers and clears finished products quickly. On transporting large products, multiple trays are subjected to form a rigid body working as a large single tray. In order to realize these movement of trays in real-time, a planning method based on the priority scheme and heuristic rule is proposed. The present method is evaluated through simulations.

1P1-D31 ペトリネットによる多様な生産システムの表現

In the flexible production system, cooperation of an operator and a robot is strongly demanded. This study uses various types of robots in a cell production system and a line production system. For the expression and analysis of the cell-line combined system and multi-robot system, we adopt the Petri-net. It has a function to represent not only the cooperation of the robot and operator, but also the special technique such as "kanban" method of the production system.

505. Advances in Large-Scale Production of Recombinant Adeno-Associated Vectors

Recombinant adeno-associated viral vectors (rAAV) are one of the most promising vectors for human gene therapy due to their long term transduction of mitotic and post-mitotic cells. In our laboratory, a novel technology for the production of rAAV has been developed. This method consists of producing rAAV in insect cells using baculovirus expression vectors. This invertebrate cell production system greatly facilitates the production and development of rAAV-based therapeutics. Although this insect cell-based production system has the potential to alleviate one of the bottlenecks in the manufacturing of rAAV, the practical aspects of large-scale production and processing of vector remains unresolved.

1315 セル生産システムに向けた小型多機能工作機械の開発(GS-13,14 生産加工・生産システム(1),研究発表講演)

In cell production system, corresponding to the variety and variate production, the small and process consolidated machine tool is needed. In this study, a desktop size multifunctional machine tool is developed. And first, basic moving performance of the development machine was measured. In addition, power consumption and thermal deformation of this mini machine tool were measured.

Challenge of Transformation to Cell Production System with Ergonomic Approach in a Small and Medium Sized Enterprise Part 2

Challenge of Transformation to Cell Production System with Ergonomic Approach in a Small and Medium Sized Enterprise Part 2

A Study of Production Optimization by Cell Production System for Assembly

A Study of Production Optimization by Cell Production System for Assembly

Ex Vivo Expansion of Bone Marrow and Cord Blood Cells to Produce Stem and Progenitor Cells for Hematopoietic Reconstitution

Ex vivo expansion of a small volume of bone marrow offers an alternate approach to cellular support for repair of a hematopoietic system damaged by radiation exposure. Unpurified bone marrow cells cultured using frequent exchange of medium (perfusion) results in (1) the growth of an adherent layer that provides a supportive microenvironment for hematopoiesis and (2) the growth of hematopoietic stem and progenitor cells. Similar increases in stem and progenitor cells were also observed in cord blood cultures using frequent medium exchange methods. An automated clinical scale system, the AastromReplicell Cell Production System, has been developed to implement the biology of this ex vivo expansion process. Clinical use of ex vivo expanded bone marrow alone or in augmenting a low dose of mobilized peripheral blood stem cells has successfully reconstituted hematopoiesis. Ex vivo expanded cord blood cells combined with unmanipulated cord blood cells have also shown significant benefits in overall survival and engraftment in pediatric and adult patients, respectively. These results suggest that ex vivo expansion of hematopoietic cells provides a mechanism for generating cells capable of hematopoietic reconstitution.

Simulation approach in comparison of a pull system in a cell production system with a push system in a conventional production system according to flexible cost: A case study

Due to globalization of the world markets, industrial firms in an increasing competitive environment are required to reduce their production costs and to produce with superior quality at short delivery times in a flexible way. The firms in the automotive industry, which have a big share in the world industry, should produce cheaper, high-quality products in short-delivery times with flexible production systems in order to be able to meet customers changing needs. In this study, a case study to apply JIT philosophy to a company that is a sub-contractor of the automotive industry will be presented. This new system and the existing system are modeled and simulated by SIMAN after which an economic analysis is performed using the simulation results.

Clinical-scale human umbilical cord blood cell expansion in a novel automated perfusion culture system.

Use of umbilical cord blood (CB) for stem cell transplantation has a number of advantages, but a major disadvantage is the relatively low cell number available. Ex vivo cell expansion has been proposed to overcome this limitation, and this study therefore evaluated the use of perfusion culture systems for CB cell expansion. CB was cryopreserved using standard methods and the thawed unpurified cells were used to initiate small-scale cultures supplemented with PIXY321,flt-3 ligand, and erythropoietin in serum-containing medium. Twelve days of culture resulted in the optimal output from most CB samples. Frequent medium exchange led to significant increases in cell (93%), CFU-GM (82%) and LTC-IC (350%) output as compared with unfed cultures. As the inoculum density was increased from 7.5 x 10(4) per cm2 to 6.0 x 10(5) per cm2, the output of cells, CFU-GM, and LTC-IC increased. Cell and CFU-GM output reached a plateau at 6.0 x 10(5) per cm2, whereas LTC-IC output continued to increase up to 1.2 x 10(6) per cm2. Because the increase in culture output did not increase linearly with increasing inoculum density, expansion ratios were greatest at 1.5 x 10(5) per cm2 for cells (6.4-fold) and CFU-GM (192-fold). Despite the lack of adherent stroma, CB cultures expressed mRNA for many growth factors (G-CSF, GM-CSF, IL-1, IL-6, LIF, KL, FL, Tpo, TGF-beta, TNF-alpha, and MIP-1alpha) that were also found in bone marrow (BM) cultures, with the exception of IL-11 (found only in BM) and IL-3 (found in neither). Culture output was remarkably consistent from 10 CB samples (coefficient of variation 0.3) indicating that the procedure is robust and reproducible. Two commercial serum-free media were evaluated and found to support only approximately 25% of the culture output as compared with serum-containing medium. Implementation of optimal conditions in the clinical scale, automated cell production system (CPS) showed that the process scaled-up well, generating 1.7 x 10(7) CFU-GM (298-fold expansion) from 1.2 x 10(8) thawed viable nucleated CB cells (n = 3). The ability to generate >10(7) CFU-GM from <15 ml of CB within this closed, automated system without the need for extensive cell manipulations will enable clinical studies to test the safety and efficacy of expanded CB cells in the transplant setting.

Cell production system for assembly

The recent diversification of market needs and shortened product life have necessitated frequent model changes which robot assembly lines can no longer accommodate. This paper proposes automatic assembly by a cell production system as a possible solution to this problem. The system is a combination of intelligent assembly cells with a conveyor system or an automatic guided vehicle system. This not only requires very little start-up time but also has high dispersibility and expansibility, because each cell can function independently whereas a robot assembles several parts. A demonstration assembly cell proved to have high flexibility and capability for long unmanned operation.