Mycobacteria are gram-positive bacteria with uncharacteristically complex cell envelopes that continue to elude our efforts to uncover their structures and properties. Understanding the structure and dynamics of the cell envelope is an essential prerequisite in creating antibiotics to combat the various diseases associated with mycobacteria, such as tuberculosis. However, the unusual complexity of the cell envelope components presents a challenge to both experimental and computational studies. To address this, we have been developing an easy-to-use website interface Mycobacterial Lipid Modeler in CHARMM-GUI and its incorporation into Membrane Builder to provide the essential components of the mycobacterial cell envelope lipids for modeling and simulation of mycobacterial inner and outer membranes with or without associated proteins.