Cell Enlargement Research Articles

The apple transcription factor MdbHLH4 regulates plant morphology and fruit development by promoting cell enlargement

The bHLH family, the second largest transcription factor (TF) family in plants, plays a crucial role in regulating plant growth and development processes. However, the biological functions and mechanisms of most bHLH proteins remain unknown, particularly in apples. In this study, we found that MdbHLH4 positively modulates plant growth and development by enhancing cell expansion. Overexpression (OE) of MdbHLH4 resulted in increased biomass, stem and root length, leaf area, and larger areas of pith, xylem, and cortex with greater cell size compared with wild-type apple plants. Conversely, RNA interference (RNAi)-mediated silencing of MdbHLH4 led to reduced xylem and phloem as well as smaller cell size compared to wild-type apple plants. Ectopic expression of MdbHLH4 in tomatoes resulted in enlarged fruits with impaired color appearance, decreased accumulation of soluble solids, and decreased flesh firmness along with larger seeds. Subsequent investigations have shown that MdbHLH4 directly binds to the promoters of MdARF6b and MdPIF4b, enhancing their expression levels. These findings suggest that MdbHLH4 potentially regulates plant cell expansion through auxin and light signaling pathways. These study results not only provide new insights into the roles of bHLH transcription factors in regulating plant growth and development but also contribute to a deeper understanding of their underlying mechanisms.

Symmetry-Broken Chern Insulators in Twisted Double Bilayer Graphene.

Twisted double bilayer graphene (tDBG) has emerged as a rich platform for studying strongly correlated and topological states, as its flat bands can be continuously tuned by both a perpendicular displacement field and a twist angle. Here, we construct a phase diagram representing the correlated and topological states as a function of these parameters, based on measurements of over a dozen tDBG devices encompassing two distinct stacking configurations. We find a hierarchy of symmetry-broken states that emerge sequentially as the twist angle approaches an apparent optimal value of θ ≈ 1.34°. Nearby this angle, we discover a symmetry-broken Chern insulator (SBCI) state associated with a band filling of 7/2 as well as an incipient SBCI state associated with 11/3 filling. We further observe an anomalous Hall effect at zero field in all samples supporting SBCI states, indicating spontaneous time-reversal symmetry breaking and possible moiré unit cell enlargement at zero magnetic field.

Sub-inhibitory concentrations of ceftriaxone induce morphological alterations and PIA-independent biofilm formation in Staphylococcus aureus

The exposure of bacteria to sub-inhibitory concentrations of antibiotics is of biological significance since it can occur in vivo under many circumstances, including low-dose treatment, poor adherence to a regimen, poor drug penetration, drug-drug interactions, and antibiotic resistance of the pathogen. In this study, we investigated the effects of subinhibitory concentrations of four antibiotics: ampicillin, ceftriaxone, gentamicin, and norfloxacin, which are commonly used in clinical settings and on cell morphology and biofilm formation in Staphylococcus aureus as one of the leading causes of nosocomial and biofilm-associated infections. Nine clinical S. aureus biofilm-producing isolates and two known biofilm-producing reference strains, S. aureus ATCC 29213 and S. aureus ATCC 6538, were used in this study. Sub-MICs of beta-lactam antibiotics (ampicillin and ceftriaxone) significantly induced biofilm formation in S. aureus ATCC 29213 and S. aureus ATCC 6538 and in six clinical isolates out of the nine selected isolates when compared with the antibiotic-free control group (P < 0.05), with an approximately 2- to 2.5-fold increase. Gentamicin and norfloxacin induced biofilms in S. aureus ATCC 29213 and S. aureus ATCC 6538, while gentamicin and norfloxacin induced biofilms only in three and two of the nine tested isolates, respectively (P < 0.05). The chemical nature of the biofilm matrix produced by half the MIC of ceftriaxone in the six isolates that showed increased biofilm was all non-polysaccharide in composition (PIA-independent). Gene expression of biofilm-encoding genes atl and sarA in biofilms of the two tested strains (S. aureus ATCC 6538) and clinical strain (S. aureus 16) showed a significant upregulation after exposure to half MIC of ceftriaxone. Additionally, the bacterial cell morphological changes in planktonic cells caused by half MIC of ceftriaxone were evaluated by scanning electron microscopy, which demonstrated a significant cell enlargement when compared with the antibiotic-free control (P < 0.05), and some deformed cells were also noticed. In S. aureus clinical isolates, sub-MICs of ampicillin, ceftriaxone, gentamicin, and norfloxacin may stimulate substantial production of biofilm, which could have important clinical significance and make infection treatment challenges. Further, in vivo research is needed to fully comprehend how sub-MIC of antibiotics can affect biofilm formation in clinical settings. Additionally, more research is required to reveal the clinical implications of the morphological alterations in S. aureus brought on by exposure to ceftriaxone at concentrations below its MIC.Graphical

The Dsup coordinates grain development and abiotic stress in rice

DNA damage is a serious threat to all living organisms and may be induced by environmental stressors. Previous studies have revealed that the tardigrade (Ramazzotius varieornatus) DNA damage suppressor protein Dsup has protective effects in human cells and tobacco. However, whether Dsup provides radiation damage protection more widely in crops is unclear. To explore the effects of Dsup in other crops, stable Dsup overexpression lines through Agrobacterium-mediated transformation were generated and their agronomic traits were deeply investigated. In this study, the overexpression of Dsup not only enhanced the DNA damage resistance at the seeds and seedlings stages, they also exhibited grain size enlargement and starch granule structure and cell size alteration by the scanning electron microscopy observation. Notably, the RNA-seq revealed that the Dsup plants increased radiation-related and abiotic stress-related gene expression in comparison to wild types, suggesting that Dsup is capable to coordinate normal growth and abiotic stress resistance in rice. Immunoprecipitation enrichment with liquid chromatography-tandem mass spectrometry (IP-LC-MS) assays uncovered 21 proteins preferably interacting with Dsup in plants, suggesting that Dsup binds to transcription and translation related proteins to regulate the homeostasis between DNA protection and plant development. In conclusion, our data provide a detailed agronomic analysis of Dsup plants and potential mechanisms of Dsup function in crops. Our findings provide novel insights for the breeding of crop radiation resistance.

RF-ShCNN: A combination of two deep models for tumor detection in brain using MRI

The tumor in the brain is the reason for jagged cell enlargement in the brain. Magnetic resonance imaging (MRI) is a common scheme to identify tumor existence in the brain. With these MRIs, the medical practitioner can examine and detect the abnormal growth of tissues and corroborate if the brain is influenced by a tumor or not. Due to the appearance of artificial intelligence models, the discovery of brain tumor is performed by adapting different models which thereby help in making decisions and selecting the most suitable diagnosis for patients. The main motivation of this work is to reduce the death rate. If they are not adequately treated, the survival rate of the patient decreases. The correct diagnoses help patients receive accurate treatments and survive for a long time. This paper develops a hybrid model, namely the Residual fused Shepherd convolution neural network (RF-ShCNN) for discovering tumor in the brain considering MRI. Thus, the Adaptive wiener filtering is adapted to filter image-commencing noise. Thereafter, Conditional Random Fields-Recurrent Neural Networks (CRF-RNN) are adapted for segmentation followed by the mining of essential features. Lastly, the features employed in RF-ShCNN for making effective brain tumor detection by means of MRI. Thus, the RF-ShCNN is built by unifying the deep residual network and Shepherd convolution neural network. The hybridization is done by adding a regression layer wherein the regression is fused with Fractional calculus (FC) to make effective detection. The RF-ShCNN provided better accuracy of 94%, sensitivity of 95% and specificity of 94.9%.

Genome homeostasis defects drive enlarged cells into senescence

Cellular senescence refers to an irreversible state of cell-cycle arrest and plays important roles in aging and cancer biology. Because senescence is associated with increased cell size, we used reversible cell-cycle arrests combined with growth rate modulation to study how excessive growth affects proliferation. We find that enlarged cells upregulate p21, which limits cell-cycle progression. Cells that re-enter the cell cycle encounter replication stress that is well tolerated in physiologically sized cells but causes severe DNA damage in enlarged cells, ultimately resulting in mitotic failure and permanent cell-cycle withdrawal. We demonstrate that enlarged cells fail to recruit 53BP1 and other non-homologous end joining (NHEJ) machinery to DNA damage sites and fail to robustly initiate DNA damage-dependent p53 signaling, rendering them highly sensitive to genotoxic stress. We propose that an impaired DNA damage response primes enlarged cells for persistent replication-acquired damage, ultimately leading to cell division failure and permanent cell-cycle exit.

Too big not to fail: Different paths lead to senescence of enlarged cells

In this issue of Molecular Cell, Crozier etal.,1 Foy etal.,2 Manohar etal.,3 and Wilson etal.4 show how excessive cell growth caused by a temporary G1 arrest leads to permanent cell cycle exit at different stages of the cell cycle.

Repurposing calcium-sensing receptor activator drug cinacalcet for ADPKD treatment

ADPKD is characterized by progressive cyst formation and enlargement leading to kidney failure. Tolvaptan is currently the only FDA-approved treatment for ADPKD; however, it can cause serious adverse effects including hepatotoxicity. There remains an unmet clinical need for effective and safe treatments for ADPKD. The extracellular Ca2+-sensing receptor (CaSR) is a regulator of epithelial ion transport. FDA-approved CaSR activator cinacalcet can reduce cAMP-induced Cl− and fluid secretion in various epithelial cells by activating phosphodiesterases (PDE) that hydrolyze cAMP. Since elevated cAMP is a key mechanism of ADPKD progression by promoting cell proliferation, cyst formation and enlargement (via Cl− and fluid secretion), here we tested efficacy of cinacalcet in cell and animal models of ADPKD. Cinacalcet treatment reduced cAMP-induced Cl− secretion and CFTR activity in MDCK cells as suggested by ∼70 % lower short-circuit current (Isc) changes in response to forskolin and CFTRinh-172, respectively. Cinacalcet treatment inhibited forskolin-induced cAMP elevation by 60 % in MDCK cells, and its effect was completely reversed by IBMX (PDE inhibitor). In MDCK cells treated with forskolin, cinacalcet treatment concentration-dependently reduced cell proliferation, cyst formation and cyst enlargement by up to 50 % without affecting cell viability. Cinacalcet treatment (20 mg/kg/day for 7 days, subcutaneous) reduced renal cyst index in a mouse model of ADPKD (Pkd1flox/flox;Ksp-Cre) by 20 %. Lastly, cinacalcet treatment reduced cyst enlargement and cell proliferation in human ADPKD cells by 60 %. Considering its efficacy as shown here, and favorable safety profile including extensive post-approval data, cinacalcet can be repurposed as a novel ADPKD treatment.

First fossil record of Trichosteleum (Bryophyta: Sematophyllaceae) from mid-Miocene Zhangpu amber in Asia

The evolutionary history of terrestrial moss in Asia during the Cenozoic is poorly interpreted in largely because of very limited fossil evidence. The middle Miocene Zhangpu amber is the sole resin source of moss fossils in China, which shows a diverse moss population during the mid-Miocene Climatic Optimum. In this study, we reported the discovery of a gametophyte fragment of moss fossil in a well-preserved amber inclusion from the middle Miocene of Zhangpu, Southeast China. The observation methods involved light microscopy and synchrotron radiation–based X-ray microcomputed tomography analysis on the amber inclusions. The fossil has lanceolate leaves with involute margins, long linear laminal cells with unipapillae, well-developed and enlarged alar cells, and an absence of costae. The fossil is assigned to the extant Trichosteleum and represents the only known occurrence of a fossilized Trichosteleum member in Asia. This finding enhances our comprehension on the moss diversity within Zhangpu amber and establishes a first record of Trichosteleum in Asia.

A transcriptional activator effector of Ustilago maydis regulates hyperplasia in maize during pathogen-induced tumor formation

Ustilago maydis causes common smut in maize, which is characterized by tumor formation in aerial parts of maize. Tumors result from the de novo cell division of highly developed bundle sheath and subsequent cell enlargement. However, the molecular mechanisms underlying tumorigenesis are still largely unknown. Here, we characterize the U. maydis effector Sts2 (Small tumor on seedlings 2), which promotes the division of hyperplasia tumor cells. Upon infection, Sts2 is translocated into the maize cell nucleus, where it acts as a transcriptional activator, and the transactivation activity is crucial for its virulence function. Sts2 interacts with ZmNECAP1, a yet undescribed plant transcriptional activator, and it activates the expression of several leaf developmental regulators to potentiate tumor formation. On the contrary, fusion of a suppressive SRDX-motif to Sts2 causes dominant negative inhibition of tumor formation, underpinning the central role of Sts2 for tumorigenesis. Our results not only disclose the virulence mechanism of a tumorigenic effector, but also reveal the essential role of leaf developmental regulators in pathogen-induced tumor formation.

Lead exposure aggravates Aβ1-42-induced microglial activation and copper ion accumulation in microglial cells

To investigate the effect of lead (Pb) exposure on Aβ1-42-induced microglial activation and copper ion accumulation in microglial cells and explore the regulatory mechanism of Pb-induced aggravation of Alzheimer's disease (AD)-like pathology. Cultured microglial BV2 cells were treated with different concentrations of Aβ1-42, lead acetate or their combination for 12 h, and the changes in cell viability and morphology were evaluated. Immunofluorescence assay was performed to detect iNOS and oxidative stress level in the treated cells, and the release of inflammatory factors was detected using ELISA. Western blotting and inductively coupled plasma-mass spectrometry (ICP-MS) were used to detect the expressions of CTR1 and ATP7A proteins and copper content in the cells. Treatment with 15 and 20 μmol/L Aβ1-42 for 12 h significantly lowered the viability of BV2 cells. Treatment with Aβ1-42 at 10 μmol/L for 12 h obviously increased the release of iNOS, TNF-α and IL-6 in the cells (P<0.05), and its combination with 15 or 20 μmol/L lead acetate more strongly lowered BV2 cell viability (P<0.05). Compared with 10 μmol/L Aβ1-42 treatment alone, 10 μmol/L Aβ1-42 combined with 10 μmol/L lead acetate for 12 h caused more obvious microglial activation, as manifested by enlarged cell bodies, increased cell protrusions and elongation, enhanced release of iNOS, TNF-α, IL-6, IL-1β and ROS, and increased intracellular copper ion accumulation and expression of copper transporter CTR1 (P<0.05). Compared with the conditioned medium from activated BV2 cells, which caused obvious injuries in hippocampal neuron HT22 cells (P<0.001), the medium from BV2 cells treated with NAC and the copper ion chelating agent TM caused milder injuries in HT22 cells (P<0.05). Lead exposure aggravates neuronal damage caused by Aβ1-42-treated microglial cells by increasing copper ion accumulation, oxidative stress, and inflammatory factor release to trigger microglial activation.

Phosphate recovery using activated sludge cyanophycin: Adsorption mechanism and utilization as nitrogen-phosphorus fertilizer

Recovery of biopolymers is an important way of resource recovery in wastewater treatment plants. However, their recycling and application pathways need to be further developed. This study explored the application potential of cyanophycin granule polypeptide (CGP) extracted from activated sludge in recycling phosphate from wastewater and used for nitrogen-phosphorus fertilizer. The results showed that the adsorption behavior of phosphate by CGP was well described by Elovich and Langmuir-Freundlich models, which reflects the uneven energy distribution on the surface of CGP and belongs to multimolecular layer adsorption. The maximal adsorption capacity of CGP to phosphate was 53.739 mg P/g, and the effects of anions and macromolecules on adsorption are negligible. The guanidyls in CGP were involved in binding phosphates, and the adsorption energies of CGP-HPO42– and CGP-H2PO4– calculated by DFT are –0.645 and –0.179 eV, respectively. Moreover, CGP absorbed phosphate significantly promoted the germination and growth of Brassica napus, and altered communities of rhizosphere rare species. Transcriptomic results of Brassica napus showed that nitrate uptake and arginine synthesis were down-regulated and arginine metabolism was up-regulated, which may be attributed to the degradation of CGP to arginine and ammonium in soil and uptake by plant. Nitrogen and phosphate released by CGP degradation promoted plant hormone signal transduction, thereby accelerating cell enlargement and plant growth. These results indicate that CGP can effectively recover phosphate and be utilized as a nitrogen-phosphorus fertilizer.

Climate response of a glacial relict conifer across its distribution range is invariant in space but not in time

Climate change impacts on forest trees will be particularly severe for relict species endemic to the subalpine forest, such as Pinus cembra in the Alps and Carpathians. Most current knowledge about the response of this species to climate comes from tree-ring width analysis. However, this approach cannot perform in-depth and highly time-resolved analysis on the climate influence on specific growth processes and xylem functions. We analyzed xylem anatomical traits from six sites covering most of the longitudinal range of this species. Associations between climate and cell number, lumen area and cell wall thickness were computed for the 1920–2010 period using climate records aligned to degree-day temperature sum thresholds. The anatomical chronologies were clearly distinct between the Alps and Carpathians. However, climate responses were similar for all sites, suggesting common species-specific response mechanisms. Temperature showed a positive correlation with both cell number and cell wall thickness. Cell lumen size exhibited an early positive association, followed by strong negative association with temperature and a positive one with precipitation. This highlights that the cell enlargement process was negatively related to high temperature at high elevation, where meristematic processes are rather supposed to be constrained by low temperatures. Therefore, long-term climate warming can have negative consequences on the xylem potential to transport water at all investigated sites. Moreover, in the last 30 years, we observed a slight anticipation of some responses and a decrease in climate sensitivity of some xylem parameters. Our findings provide evidence of temporally unstable but spatially consistent climate response of Pinus cembra from the Alps to the Carpathians. The low diversity in xylem phenotypic responses to climate suggests that future warming could extensively and evenly affect the species throughout its entire distribution.

Chemistry and electrochemistry of CeO&lt;sub&gt;2&lt;/sub&gt;-based interlayers: Prolonging the lifetime of solid oxide fuel and electrolysis cells

Research and development of solid oxide fuel cells (SOFCs) and solid oxide electrolysis cells (SOECs) are currently of paramount importance in terms of realizing hydrogen energy and carbon emission reduction programs, which many countries have committed to. Although, there are many outstanding results in the fabrication and characterization of SOFCs and SOECs with promising oxygen-ionic and proton-conducting electrolytes, conventional zirconia electrolytes are still widely used not only in a lab-scale setup, but also in the form of enlarged cells and stacks, with the experimental operation of the latter during 10 000–100 000 h. To ensure good performance stability and microstructural integrity of such multilayered cells, a special attention should be paid to the chemical activity of functional materials toward their interaction with each other, especially in long-term focus. The literature analysis has shown that many undesirable processes occur in SOFCs and SOECs with the classical pairs of zirconia electrolytes and strontium-containing electrodes, including element segregation and interdiffusion, insulating phase formation, microscopic defect appearance, and delamination. Some of these processes can be efficiently eliminated by using so-called interlayers designed from doped ceria materials. Due to their numerous beneficial functions, such interlayers have several synonymous names: blocking, barrier, buffer, or protecting layers. Herein, we review the recent progress and achievements in the fundamental and applied researches dealing with the ceria interlayers and their impact on chemistry and electrochemistry of solid oxide cells based on classical zirconia electrolytes as well as promising oxygen-ionic and proton-conducting analogs. &lt;br&gt; The bibliography includes 405 references.

Affimer-mediated locking of p21-activated kinase 5 in an intermediate activation state results in kinase inhibition

Kinases are important therapeutic targets, and their inhibitors are classified according to their mechanism of action, which range from blocking ATP binding to covalent inhibition. Here, a mechanism of inhibition is highlighted by capturing p21-activated kinase 5 (PAK5) in an intermediate state of activation using an Affimer reagent that binds in the P+1 pocket. PAK5 was identified from a non-hypothesis-driven high-content imaging RNAi screen in urothelial cancer cells. Silencing of PAK5 resulted in reduced cell number, G1/S arrest, and enlargement of cells, suggesting it to be important in urothelial cancer cell line survival and proliferation. Affimer reagents were isolated to identify mechanisms of inhibition. The Affimer PAK5-Af17 recapitulated the phenotype seen with siRNA. Co-crystallization revealed that PAK5-Af17 bound in the P+1 pocket of PAK5, locking the kinase into a partial activation state. This mechanism of inhibition indicates that another class of kinase inhibitors is possible.

Therapeutic Potential of Enzymatically Extracted Eumelanin from Squid Ink in Type 2 Diabetes Mellitus (T2DM) ICR Mice: Multifaceted Intervention against Hyperglycemia, Oxidative Stress, and Depression.

Type 2 diabetes mellitus (T2DM) is a prevalent metabolic disease that poses significant health risks due to its numerous complications. However, the effects of eumelanin on oxidative stress, hyperglycemia, and depression in diabetic mice haven't been extensively studied. Our study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline-neutral-flavor protease (ANF) displayed superior inhibitory activity against α-glucosidase and α-amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance, and modulating total cholesterol (TC), low-density lipoprotein (LDL) levels, as well as antioxidant indices (SOD, GSH-Px, and MDA) at a dosage of 0.04 g·kg-1 . Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice. Overall, these findings underscore the significant therapeutic potential of ANF in the treatment of T2DM and its associated complications. By augmenting lipid and glucose metabolism, mitigating oxidative stress, and alleviating depression, ANF emerges as a promising candidate for multifaceted intervention. This article is protected by copyright. All rights reserved.

Atypical cystic hypersecretory lesions of the breast commonly harbour TP53 alterations.

Cystic hypersecretory lesions are rare and include atypical cystic hypersecretory hyperplasia (A-CHH) and cystic hypersecretory carcinoma in situ (CHC-IS). Despite detailed morphological descriptions, little is known about the genetic landscape of these lesions. We identified four A-CHH and three CHC-IS from 2010 to 2022. Patients ranged from 39 to 65 (median 49) years. All lesions showed characteristic cystically dilated ducts with colloid-like secretions lined by enlarged cells with hyperchromatic nuclei and at least moderate cytological atypia. CHC-IS was remarkable for a greater degree of intraductal proliferation, typically with a micropapillary pattern. Four patients had concurrent ipsilateral invasive carcinoma. Next-generation sequencing (104 cancer-associated genes) was successful in four, showing variants in TP53 (3), KEAP1 (1) and MDM2 (1). p53 immunohistochemistry was concordant with molecular results with mutant-pattern staining in three TP53-mutants and wild-type in one. In three cases where sequencing failed, one showed mutant p53 staining, one was wild-type and one had no remaining lesion. The combined molecular and immunohistochemical results demonstrated p53 alterations in one A-CHH and three CHC-IS. Based on this limited cohort, atypical cystic hypersecretory lesions appear to commonly harbour TP53 alterations. To our knowledge, this is the first study to characterise molecular alterations in this rare subset of breast lesions.

The Effect of Wharton's Jelly-Derived Conditioned Medium on the In Vitro Maturation of Immature Oocytes, Embryo Development, and Genes Expression Involved in Apoptosis.

Oocyte cytoplasmic maturation is a crucial process during in vitro maturation (IVM), and finding an appropriate IVM medium that promotes oocyte competence is very critical in assisted reproductive technology (ART). The aim of this study was to investigate the effects of umbilical cord Wharton's jelly (WJ-MSCs)-derived conditioned media on the maturation of immature oocytes and their developmental potential in humans after IVM, as well as apoptotic gene expression. A total of 392 germinal vesicle (GV) oocytes were collected from 207 women aged 25-35 years and divided into two IVM groups: (1) control group, which was cultured in CleavTM medium, and (2) experimental group, which was cultured in supernatants of umbilical cord Wharton's jelly as a conditioned medium (CM). First, WJ-MSCs were isolated, and their purity was analyzed. The immunophenotypes of WJ-MSCs were analyzed by flow cytometry. The quantitative expression of BCL2, BAX, and BAG1 in matured oocytes and embryos was evaluated through quantitative real-time polymerase chain reaction (qRT-PCR). Our findings showed that WJ-MSCs have a high proliferating capacity. The purity of the isolated cells was further validated by immunophenotyping, which revealed that their surface antigen expression had phenotypical properties similar to WJ-MSCs. When compared to CD34 and CD45 surface markers, the enlarged cells were positive for CD90, CD105, and CD44. There were significant differences in cytoplasmic maturation of oocytes and embryo quality between the two groups. The mRNA expression levels of BCL-2, BAG1, and BAX in matured oocytes and embryos were also significantly different between the two groups. Therefore, WJ-MSCs medium indicated potential efficacy in terms of ameliorating oocyte maturation and in promoting the development and genes expression of BAX, BCL-2, and BAG1.

Paired growth of cultivated and halophytic wild rice under salt stress induces bacterial endophytes and gene expression responses.

Utilizing salt-affected marginal lands in coastal regions can help meet the growing demand for rice. We explored a nature-based solution involving wild halophytic rice (O. coarctata, Oc) and commercial rice BRRI Dhan 67 (O. sativa, Os) grown in close proximity to each other under salt stress. This was to investigate whether a paired planting strategy could help complement rice growth and yield under stress. We also investigated the gene expression and endophytic bacterial profiles of both Os and Oc in unpaired and paired conditions without and with salt. Paired plants exhibited lower salt damage indicators such as smaller reduction in plant height, electrolyte leakage and chlorophyll loss, as well as higher K+/Na+ ratio under saline stress. Some of the 39 endophytic bacteria in the mutualism experiment were unique to Oc and transferred to Os when paired. Differentially expressed genes in leaves of paired Os versus unpaired Os were 1097 (994 up-regulated, 101 down-regulated) without salt and 893 (763 up-regulated, 130 down-regulated) under salt stress. The presence of Oc plants under salt stress influenced major biological processes in Os, including oxidative stress; chitinase activity; phenylalanine catabolic process and response to ABA. Protein binding and serine/threonine kinase activity were primarily affected in molecular function. The downregulated WRKY transcription factor 22 in paired conditions under salt stress played a role in the MAPK signaling pathway, reducing respiratory cell death. The upregulated auxin-responsive protein IAA18 gene, involved in hormone signaling and cell enlargement, was present only in paired plants. Our findings therefore, offer insights into developing more effective cultivation strategies for sustainable rice production.

Giant liposarcoma of esophagus: a rare case report

BackgroundLiposarcoma is a malignant mesenchymal tumor that most commonly involves the retroperitoneum and lower extremities. However, liposarcoma of esophagus has been rarely reported in the literature.Case presentationWe report a case of a 46-year-old man with complaint of intermittent dysphagia for 6 years, accompanied with paroxysmal vomiting of pedicled tumor to the mouth. Imaging studies showed a huge mixed density lesion in the middle esophageal lumen. Surgical resection of the tumor was performed through an external cervical approach. Microscopically, the tumor was composed of mature adipocytes in normal adipose tissue prominently intersected by sparsely cellular fibrous septa containing atypical, enlarged spindle cells with hyperchromatic nuclei. Immunohistochemically, the tumor cells were positive for Vimentin, S-100, CD34 and MDM2. Besides, fluorescence in situ hybridization (FISH) analysis indicated the presence of amplification involving MDM2 gene. The patient was diagnosed as having esophageal well-differentiated liposarcoma and recovered well after the operation.ConclusionsEsophageal liposarcoma is an extremely rare tumor. Due to the nonspecific clinical manifestation and lack of experience, it is challenging to make a clear diagnosis before operation. Definite diagnosis of esophageal liposarcoma depends on histopathology, immunohistochemistry and molecular analysis.