Celery Seeds Research Articles

Celery Seed and Related Extracts with Antiarthritic, Antiulcer, and Antimicrobial Activities.

Celery preparations have been used extensively for several millennia as natural therapies for acute and chronic painful or inflammatory conditions. This chapter reviews some of the biological and chemical properties of various celery preparations that have been used as natural remedies. Many of these have varying activities and product qualities. A fully standardized celery preparation has been prepared known as an alcoholic extract of the seeds of a plant source derived from northern India. This is termed, Celery Seed Extract (CSE) and has been found to be at least as effective as aspirin, ibuprofen, and naproxen in suppressing arthritis in a model of polyarthritis. CSE can also reduce existing inflammation in rats. CSE has also been shown to provide analgesia in two model systems. CSE, in addition to acting as an analgesic and inflammatory agent, has been shown to protect against and/or reduce gastric irritation caused by NSAIDs, as well as act synergistically with them to reduce inflammation. The CSE was fractionated by organic solvent extractions, then subjected to column chromatography followed by HPLC and was characterized by mass spectrometry. This yielded a purified component that had specific inhibitory effects on Helicobacter pylori but was not active against Campylobacter jejuni or Escherichia coli. Additionally, toxicology studies did not reveal any clear signs of toxicity at doses relevant to human use. Also, unlike many dietary supplements, the available data suggest that CSE does not significantly affect the p450 enzyme systems and thus is less likely to alter the metabolism of drugs the individual may be taking. CSE may be a prototype of a natural product that can be used therapeutically to treat arthritis and other inflammatory diseases.

Celery Seed Extract Blocks Peroxide Injury in Macrophages via Notch1/NF-κB Pathway.

Oxidized low-density lipoprotein (ox-LDL)-induced macrophage foam cell formation and injury is one of the major atherogenic factors. This study is aimed to investigate the protective effect of celery seed extract (CSE) on ox-LDL-induced injury of macrophages and the underlying signaling pathway. RAW264.7 macrophages were pre-incubated with CSE for 24 h, followed by stimulation with ox-LDL. Oil red O staining and enzymatic colorimetry indicated CSE significantly lessened lipid droplets and total cholesterol (TC) content in ox-LDL-injured macrophages. ELISA revealed that CSE decreased the secretion of inflammatory cytokine TNF-α and IL-6 by 12-27% and 5-15% respectively. MTT assay showed CSE promoted cell viability by 16-40%. Cell apoptosis was also analyzed by flow cytometry and laser scanning confocal microscope and the data indicated CSE inhibited ox-LDL-induced apoptosis of macrophages. Meanwhile, western blot analysis showed CSE suppressed NF-κBp65 and notch1 protein expressions stimulated by ox-LDL in macrophages. These results suggest that CSE inhibits ox-LDL-induced macrophages injury via notch1/NF-κB pathway.

Protective effect of 3-n-butylphthalide against hypertensive nephropathy in spontaneously hypertensive rats.

Previous studies have demonstrated that a natural product of celery seeds, 3‑n‑butylphthalide (NBP), has significant antihypertensive effects that are widely utilized in Chinese traditional medicine. The present study aimed to investigate the effects of NBP on hypertensive nephropathy, as well as the mechanisms underlying this disease in spontaneously hypertensive rats (SHRs). SHRs were treated orally with saline, NBP (15 or 30 mg/kg) or losartan (10 mg/kg) daily for 20 weeks, during which time blood pressure was measured every four weeks. At the end of the 20‑week treatment, blood and urine samples were collected for biochemical analysis, and kidney tissues were obtained for histopathological analysis and immunohistochemistry. Enzyme‑linked immunosorbent assays and western blotting were used to analyze the expression of transforming growth factor (TGF)‑β1 in blood and kidney tissues, respectively. The results showed that NBP effectively attenuated progression of hypertensive nephropathy by decreasing urinary albumin excretion and blood urea nitrogen levels. It significantly decreased blood pressure (although less markedly than losartan) and the incidence of glomerulosclerosis. In addition, it alleviated tubular impairment and significantly decreased oxidative stress, as well as the expression of pro‑inflammatory cytokines and TGF-‑β1 in kidney tissues. In conclusion, the results suggested that NBP may slow the progression of hypertensive nephropathy by a variety of mechanisms.

Larvicidal, Repellent, and Irritant Potential of the Seed-Derived Essential oil of Apium graveolens Against Dengue Vector, Aedes aegypti L. (Diptera: Culicidae).

Aedes aegypti L. is one of the primary disease vectors spreading various dreadful diseases throughout the world, specifically over tropics and subtropics. Keeping in view the adverse effects of chemical insecticides-based intervention measures, the eco-friendly and bio-degradable essential oil extracted from the seeds of celery, Apium graveolens were investigated for its efficacy against Ae. aegypti. Larvicidal bioassay carried out with the seed oil against early fourth instars of Ae. aegypti caused an LC50 and LC90 values of 16.10 and 29.08 ppm, respectively, after an exposure to 24 h. The cidal effect of the celery seed oil augmented by 1.2-fold; after an exposure to 48 h; revealing an LC50 value of 13.22 ppm. Interestingly, the seed oil did not cause immediate larval mortality, suggesting a delayed toxicity against the larval stage. Present investigations also revealed remarkable effective repellency of the oil leading to 100% protection till 165 min as compared to control that did not result in any repellency against adult Ae. aegypti. Interestingly, only one bite was recorded in the 165th-min after which only two bites were scored until 180 min of exposure of the adult mosquitoes to the oil. An exciting observation was that the knocked-down effect in adults exposed to 10% oil-impregnated papers. The contact irritancy assays with paper impregnated with 1% celery seed oil caused first flight only after 4 s resulting in an average of 63.66 flights during 15 min of exposure revealing the relative irritability of 26.97. The qualitative phytochemical analysis of the seed oil showed the presence of flavonoids, lactones, and terpenoids as the major constituents suggesting their probable role in the toxicity. Our results confirmed that celery seed essential oil can be used as an efficient larvicide and repellent against Ae. aegypti. The identification of the bioactive components, their mode of action, and studying effects on non-target organisms and the environment would help in devising mosquito-management strategies.

Effects of L-3-n-butylphthalide on cognitive dysfunction and NR2B expression in hippocampus of streptozotocin (STZ)-induced diabetic rats.

Diabetes mellitus is associated with rapid cognitive decline. Currently, there is no effective treatment for cognitive dysfunction induced by diabetes. L-3-n-Butylphthalide (L-NBP) is a nerve protective drug extracted from seeds of celery, which has been proved to improve learning and memory in vascular dementia animal models by improving microcirculation, protecting mitochondria and increasing long-term potentiation (LTP). NR2B, one of the subunits of N-methyl-D-aspartate receptor, has been proved to be an important factor for the formation of LTP. The study aimed to investigate the role of NR2B in cognitive dysfunction in the rats with type 1 diabetes and define the protective effects of L-NBP on cognition. A rat model of type 1 diabetes was established by a single intraperitoneal injection of streptozotocin at 60 mg/kg. Animals were randomly allocated to four groups: normal control (NC); diabetic control (DC); diabetic + low L-NBP (DL, administered L-NBP 60 mg/kg per day for 12 weeks); and diabetic + high L-NBP (DH, administered L-NBP 120 mg/kg per day, for 12 weeks). After 12 weeks, cognitive and memory changes were investigated in the Morris water maze. The expression of NR2B was assessed by real-time polymerase chain reaction, Western blotting, and immunohistochemistry. Our results indicated that the escape latency was significantly increased and the number of crossing platform was significantly decreased in DC group compared to NC group. Also, the expression of NR2B was significantly declined in DC group. However, compared to DC group, the expression of NR2B of the L-NBP-treated groups was significantly increased and the escape latency was shortened with the DH group being the most obvious. Therefore, L-NBP can improve the cognitive function by up-regulating the expression of NR2B in STZ-diabetic rats, which may provide the direction for future diabetic encephalopathy therapy.

Optimization of Microwave Assisted Process for Extraction of Celery Seed Essential Oil

Microwave assisted extraction (MAE) method was developed for extraction of essential oil from celery seeds. A domestic microwave oven was modified and Clevenger apparatus attached to it to make it an extraction unit. Effect of various parameters such assoaking time, temperature and power density during MAE was studied.Amultivariate study based on a Box-Behnken design was used to evaluate the influence of three major variables (soaking time, temperature and power density) affecting the performance of MAE on celery seed. Oil yield, time of extraction and energy consumption (MJ.kg-1 oil) by MAE were determined and compared with those obtained by the traditional hydro-distillation (HD). It was found that microwave assisted process gave approximately same oil yield (1.90%) in less time ( 93.5 min) and with low energy consumption (58191.78 MJ.kg-1 oil). Results revealed that the selected parameters had significant effect on the responses.

Ninety-day administration of dl-3-n-butylphthalide for acute ischemic stroke: a randomized, double-blind trial

Background Dl-3-n-butylphthalide (NBP), first isolated from the seeds of celery, showed efficacy in animal models of stroke. This study was a clinical trial to assess the efficacy and safety of NBP with a continuous dose regimen among patients with acute ischemic stroke. Methods A randomized, double-blind, double-dummy trial enrolled 573 patients within 48 hours of onset of ischemic stroke in China. Patients were randomly assigned to receive a 14-day infusion of NBP followed by an NBP capsule, a 14- day infusion of NBP followed by aspirin, or a 14-day infusion of ozagrel followed by aspirin. The efficacy measures were Barthel index score and the modified Rankin scale (mRS) at day 90. Differences among the three groups on mRS were compared using χ2 test of proportions (with two-sided α=0.05) and Logistic regression analysis was conducted to take the baseline National Institutes of Health Stroke Scale (NIHSS) score into consideration. Results Among the 535 subjects included in the efficacy analysis, 90-day treatment with NBP was associated with a significantly favorable outcome than 14-day treatment with ozagrel as measured by mRS (P <0.001). No significant difference was found among the three groups on Barthel index at day 90. The rate of adverse events was similar among the three groups. Conclusions The 90-day treatment with NBP could improve outcomes at the third month after stroke. The NBP treatment (both intravenous and oral) is safe (ChiCTR-TRC-09000483).

Enrichment of Bio-Active Phthalides in Celery Seed Oil

Objective: To develop an efficient process to obtain a phthalide-enriched nutraceutical fraction from celery seed oil.Methods: Three approaches viz., fractional distillation, column chromatography and solvent-solvent partition, have been used.Results: Fractional distillation of celery seed oil (13.7g) afforded i) a limonene-rich fraction (7.6g, 97% purity), ii) a fraction containing β-selinene (2.8 g, 90 % purity) and iii) one containing phthalides (2.9 g, 90 % purity). Solvent-solvent partition of celery seed oil gave limonene (87%) and a fraction containing phthalides (49%), which on further fractionation afforded a phthalide-enriched fraction (90%). By conventional silica gel column chromatography, a product rich in phthalides (53-74%) could be obtained.Conclusion: Fractional distillation is shown to be a viable method to process celery seed oil into a phthalide-enriched product with high nutraceutical potential.

Condiments before Claudius: new plant foods at the Late Iron Age oppidum at Silchester, UK

Our understanding of the introduction and adoption of new plant foods in Roman Britain is currently limited by a lack of data from a group of Late Iron Age settlements commonly referred to as oppida (large pre-Roman towns). This paper presents the first evidence of several imported plant foods from Late Iron Age Britain in the form of waterlogged plant remains from the oppidum at Silchester. These were recovered from the basal contexts of two wells, dated to the early first century a.d. One olive stone and several seeds of celery, coriander and dill were identified. The results are compared to archaeobotanical data from elsewhere in Britain and northwestern Europe, demonstrating that Silchester is part of the wider phenomenon of the adoption of new flavourings and fruits in Late Iron Age Europe.

Antihypertensive Effect of Celery Seed on Rat Blood Pressure in Chronic Administration

This study investigated the effects of different celery (Apium graveolens) seed extracts on blood pressure (BP) in normotensive and deoxycorticosterone acetate-induced hypertensive rats. The hexanic, methanolic, and aqueous-ethanolic extracts were administered intraperitoneally and their effects on BP and heart rate (HR) were evaluated in comparison with spirnolactone as a diuretic and positive control. Also, the amount of n-butylphthalide (NBP), as an antihypertensive constituent, in each extract was determined by HPLC. The results indicated that all extracts decreased BP and increased the HR in hypertensive rats, but had no effect on normotensive rats. The data showed that administration of 300 mg/kg of hexanic, methanolic, and aqueous-ethanolic (20/80, v/v) extracts of the celery seed caused 38, 24, and 23 mmHg reduction in BP and 60, 25, and 27 beats per minute increase in the HR, respectively. Also, the HPLC analysis data revealed that the content of NBP in the hexanic extract was 3.7 and 4 times greater than methanolic and aqueous-ethanolic extracts. It can be concluded that celery seed extracts have antihypertensive properties, which appears to be attributable to the actions of its active hydrophobic constitutes such as NBP and can be considered as an antihypertensive agent in chronic treatment of elevated BP.

Growth-disrupting, larvicidal and neurobehavioral toxicity effects of seed extract of Seseli diffusum against Aedes aegypti (L.) (Diptera: Culicidae)

Plant extracts that kill, deform the post-embryonic molting stages of mosquitoes could be a valuable approach in integrated vector management programmes to replace synthetic chemical pesticides, containing persistent toxic substances. Crude ethanolic seed extract was obtained by maceration of seeds of Indian celery, Seseli diffusum (Apiaceae). The ethanolic seed extract induced strong neurobehavioral toxicity against the 4th instar larvae of Aedes aegypti which resulted in a high level of larval knock-down with EC50 of 238.15ppm, after 4h of treatment. The extract at concentration of 200ppm exhibited a potent larvicidal effect against 4th instar A. aegypti, produced 88% and 96% mortality, after 24 and 48h of treatment with LC50 of 126.13ppm and LC50 of 112.53ppm, respectively. The ethanolic seed extract at higher concentration (>400ppm) produced 100% mortality, after 24h and 48h of treatment. At lower concentration of 100ppm, extract induced a high level of morphological deformities in larvae, after 48h of treatment. A high level of growth and/or molting disrupting effect of extract against larvae was also observed which resulting in the formation of larval–pupal intermediates, after 48h of treatment. At concentrations of 200–300ppm, ethanolic seed extract induced morphological deformities in dead pupae, after 72h of treatment. The results showed that S. diffusum is a promising candidate for the development of new botanical biopesticide having multiple potentials for controlling insect pest of medical and agricultural importance.

Phytopathogenic Fungal Inhibitors from Celery Seeds

Extract of celery (Apium graveolens L.) seeds was investigated against phytopathogenic fungi. The light petroleum extract showed promising inhibition activities in the tests against Rhizoctonia solani and Fusarium oxysporum f. sp. vasinfecum. Chromatographic separation of the extract gave 19 fractions, one of which, QCZ-4, possessed significant inhibitory rates of 64.6%, 88.4% and 54.7% at a concentration of 100 ppm against R. solani, F. oxysporium f. sp. vasinfecum and Alternaria alternata, respectively. Major components in the active fraction were identified by GC-MS as p-(2-aminoethyl)phenol (39.7%), 3-(3,4-dimethybenzoyl) propionic acid (32.6%) and p-heptylphenol (26.9%).

Nutraceutical potential and bioassay of Apium graveolens L. grown in Khyber Pakhtunkhwa-Pakistan

The present study investigates the nutritive and pharmacological potential of Apium graveolens commonly available as wild plant in Peshawar and suburbs of Khyber Pakhtunkhwa region of Pakistan. For this purpose, plants of A. graveolens L. was collected from its natural habitat at mature stage (seedling stage) from Palusi, near KPK Agricultural University, Peshawar Pakistan. Similarly, celery seeds were obtained from 3 different ecological zone markets of Khyber Pakhtunkhwa including Peshawar, Swat and Dera Ismail Khan Regions of KPK Pakistan. Analysis of the data revealed that all the examined plants contained appreciable amount of moisture, ash, fat, protein and fiber. The wild celery had good level of vitamin C and β-carotene. The phytochemical screening of methanolic extracts of celery showed the presence of various groups of compounds including tannins, steroids, phenolic acids, terpenoids, flavonoids, volatile oils and saponins, whereas cardiac glycosides was absent in all the samples. Among the antioxidant parameters, a significant amount of total phenols and tannins were observed. Likewise elements analyzed were in the order of Na> P> K> Ca> Mg> Fe> Cu> Mn> Zn> Ni> Pb> Pt> Cd> Se> Cr. The results further revealed that Mg, Ca, P, K and Na were present in fairly high amount. The methanolic extract of all the examined celery showed positive antibacterial activity against different strains tested. Similarly, antifungal potential of the celery was determined againstTrichphyton longifuss, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani and Candida glabrata in concentration 200 µg ml-1 of dimethyl sulfoxide(DMSO). The results revealed that all the organic solvents (hexane, chloroform, methanol) and water extracts showed no brine shrimp lethality and leishmanicidal activity. Key words: Bioassay, proximate, antibacterial, antifundal, Apium graveolens.

Effects of strawberry leaves and celery seeds extracts in terlipressin-induced chronic hyponatremia in rats

Effects of strawberry leaves and celery seeds extracts in terlipressin-induced chronic hyponatremia in rats

Chemical Composition and Ability of Scavenging DPPH Radical of Essential Oil and Residue from the Celery Seed

The chemical composition of essential oil (1.18% yield) from celery seeds by hydrodistillation extraction method was analyzed by GC-MS. A total of 23 constituents were detected, in which 18 compounds that account for 91.38% were identified. The main constituents were limonene (31.15%), β-selinene (22.28%) , p-cresyl isovalerate (14.94%), α-2-propenylbenzenemethanol (9.872% ), β-myrcene (4.32%) and α-selinene (4.32%). The abilities of scavenging DPPH radical of the essential oil and hydrodistillation residue were studied. The result indicated that the maximum scavenging rates was 98.1% when 0.20 mL of the oil was used. The extracts of residue by methanol, ethanol and acetone also has remarkable scavenging activity. The result showed that the scavenging rates of the methanol, ethanol and acetone extracts of the residue were 91.5%, 91.2% and 56.2% respectively, when 0.10 mL of the extract solutions was used.

The inhibitory effect of some medical plants on the growth of some bacterial isolates from respiratory infections

This study was carried out to evaluate seven types of herbs being: black pepper seeds (Piper nigrum), celery seeds (Apium graveolens), cubeb seeds (Piper cubeba), camphor seeds (Cinnamomuum camphora), myrrh (Commiphora mol mol), fenugreek seeds (Trigonella foenum- graecum) and seeds of Lepidium sativum. The antibacterial activity of alcoholic extracts of these plants was performed on gram- positive and gram- negative bacteria isolated from human respiratory tract infections including : S. aureus ,S. pneumoniae, M. antarcticus, Corynebacterium spp. and coagulase- negative Staphylococci (CNS). Gram –negative bacteria included: K. oxytoca, K. pneumoniae and N. meningitidis, P. aeruginosa and E. coli. We studied the bacterial sensitivity to 10 antibiotics: Tobramycin (TOB), Ceftazidime (CAZ), Vancomycin (VA), Ampicillin (AM), Ofloxacin (OFX), Cloxacillin (CX), Cefalothin (KF), Erythromycin (E), Oxytetracycline (T), Amoxicillin/Clavulanic acid (CTX). Cubeba essential oil was superior in inhibiting all the examined bacteria compared with other plants. K. oxytoca was most sensitive to Lepidium sativum seeds extract followed by myrrh and fenugreek, while K. pneumoniae was most sensitive to cubeba and camphora. Cubeba followed by myrrh pi spp. was resistant to 5 antibiotics and sensitive to 4 which were: TOB, VA, OFX and KF.

Extension de la pourriture molle du céleri branche en maraîchage périurbain de Yaoundé (Cameroun)*

Celery, Apium graveolens var. dulce, is the most important leafy vegetable crop in the Nkolondom inland valley, which supplies the majority of vegetables destined for sale in Yaoundé (Cameroon). Celery is a traditional leafy vegetable, but in recent years the crop has been threatened by the extension of soft decay which reduces the period during which it can be grown by one third. As the bacterium Pectobacterium carotovorum, formerly called Erwinia carotovora, was identified as the causal agent, the harvest technique, which involves the phased removal of individual leaves, was suspected of facilitating the spread of the disease through scars left on the stems. Local cultivation practices were compared with standard practices in temperate regions (where celery originated), which differ in sowing, planting, and harvesting techniques. The results showed, firstly, that the extension of the disease was not due to the local harvesting technique, which is well adapted to the local market, and secondly, that better yields are obtained using the local technique. The recent extension of the disease could be due to a change in the variety of celery, which was introduced at the same time as the extension of the disease became apparent. The new variety was planted because of difficulties in importing celery seeds, thus highlighting the inherent risk of any poorly organized innovation in a vegetable sector.

Abstract 1879: Chemoprevention of rat mammary carcinogenesis by Apiaceae spices: Potential mechanisms

Abstract Spices have been used for generations in cuisines, as well as in the treatment of various ailments as part of folklore medicine. Scientific evidence suggests that many herbs and spices have medicinal properties that alleviate symptoms or prevent disease. We have previously demonstrated that certain Apiaceae spices (fennel, cumin and ajowan) are protective against oxidative DNA adducts and estrogen-induced changes in the ACI rat mammary tumorigenesis model. Here we report on the chemopreventive potential of other Apiaceae spices with presumable distinct phytochemical profiles, namely, anise, caraway and celery seeds and their possible mechanisms, along with the previously studied spices. Groups of female ACI rats were given either control (AIN 93M) diet or diet supplemented with 7.5% (w/w) anise, caraway and celery seeds. Two weeks later, one half of the animals in each groups were treated with subcutaneous silastic implants of 17ß-estradiol (1.2 cm; 9 mg). Rats received water and diet ad libitum. Diet intake and body weight were recorded weekly and animals were euthanized after 3 and 12 weeks. No significant difference was found in the diet intake and body weight gain suggesting that test spices were well tolerated at given doses. Estrogen treatment showed significantly (2.1 and 3.4 fold) enhanced growth of pituitary gland at 3 and 12 weeks, respectively. However all test spices significantly offset the pituitary growth by 12 weeks, except celery which was effective as early as 3 weeks. All test spices also significantly reduced the plasma prolactin level by 3 weeks, and this protection was more pronounced by 3 months, with celery eliciting the highest protection. Immunohistochemical analysis of PCNA in mammary tissues from three weeks studies has shown significant reduction in estrogen-mediated mammary cell proliferation by fennel, ajowan and cumin, analyzed thus far. To determine potential molecular targets of the spices, in the genomic and non-genomic pathways of estrogen-mediated mammary tumorigenesis, we analyzed mammary tissues for selected proteins by Western blot analysis. Diets supplemented with fennel, ajowan, caraway and cumin significantly offset estrogen-mediated overexpression of both cyclin D1 and ERα. Likewise the expression of CYP1B1 by Western analysis and CYP1A1 by RT-PCR were also found to be inhibited by fennel, ajowan and cumin. These data suggest that Apiaceae spices offset estrogen-mediated increases in i) mammary cell proliferation; ii) ERα; iii) cyclin D1; and iv) CYP1A1 and CYP1B1. Noteworthy is the reduction of circulating prolactin levels by the spices, which is one of the key factors in mammary tumorigenesis in this model. (Supported from the USPSH grant CA-125152 and Agnes Brown Duggan Endowment). Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1879.

Chemical Synthesis of 9(Z)-Octadecenamide and Its Hypolipidemic Effect: A Bioactive Agent Found in the Essential Oil of Mountain Celery Seeds

The unusual hypolipidemic activity of the methanolic fractionate of the essential oil (EOM) obtained from the mountain celery seed was previously reported. The most enriched 9(Z)-octadecenamide (oleamide) was speculated to be responsible for the relevant bioactivity. Chemically syntheized oleamide (CSO) yielded 85.1% with a purity of 98.6% when identified by RP-HPLC, FTIR, HREIMS, (1)H NMR, and (13)C NMR. CSO was tested for its antioxidative and hypolipidemic bioactivities. Results indicated CSO was potently hypolipidemic with regard to serum TG, TC, LDL-C, LDL-C/HDL-C, and hepatic TG (p < 0.05), but not for serum HDL-C and hepatic TC. In addition, CSO exhibited only poor antioxidative activity, implicating the possibility that the hypolipidemic and antioxidative bioactivity of original EOM was due to another coexisting constituent, probably gamma-selinene. Conclusively, oleamide is a potent hypolipidemic agent as regarding its effects on decreasing serum TG, TC, LDL-C and hepatic TG.

Penelitian Fiksasi Rasa Dan Aroma Seledri

The research was aimed to study the quality changes of ;1avor fixative of celery leaves and celery seeds during storage and their acceptance by consumers. Two fixation formulas from two kinds of raw material were made i.e. (a) cellulose microcrystalline/sorbitol and (b) cellulose microcrystalline/sucrose. Shelf life of fixative products were tested at 0,30 and 60 days for their moisture contents, aroma stability, microbes and preference test on aroma and taste. The products of sorbitol from celery seed oleoresin and sucrose from celery seed were relatively better than these from celery leaves oleoresin because the moisture fixative of sorbitol and fixative of sucrosa 5,82 %, and 2,19 % respectively. The reduction of aroma of fixative of sorbitol was 3, 11 % and fixative of sucrose was 3, 79 % after 60 days of storage. The panel test from 12 panelists showed that products using sorhitol from the celery leaves 11ere preferred because they gave the result of 6 - 6,33.