Seed Extract of Apium Graveolens Protects Against Cisplatin-Induced Acute Hepatotoxicity in Rats

Abstract

Original Article ​ ​ Year: 2023 I Volume: 5 I Issue: 3 I Pages I 1-12 ​ ​ Seed Extract of Apium Graveolens Protects Against Cisplatin-Induced Acute Hepatotoxicity in Rats ​ Naglaa Raafat AbdRaboh 1,2*, Amina Mahdy 3, Yosra Lozon 4 ​ 1 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. 2 Department of Biochemistry, Dubai Medical College, Dubai, United Arab Emirates. 3 Department of Medical Pharmacology, International School of Medicine, Istanbul Medipol University, Istanbul, Turkey. 4 Department of Clinical Pharmacy, Dubai Pharmacy College, Dubai, United Arab Emirates. ​ * Corresponding Author: ​ Naglaa Raafat AbdRaboh, MD, PhD Email address: naglaoda@hotmail.com ​ Source of funding: None Conflict of interest: None ​ Submission date: 12 September 2023 ​ Acceptance date: 26 September 2023 ​ Get permission for commercial use. ​ Key words: Cisplatin, Celery Seed Extract, Interleukins, Glutathione peroxidase, Hepatotoxicity, Prostaglandin. ​ ​​ Abstract: ​ Background: Cisplatin (CP) is extensively used in treating neoplasms; with serious side effects including hepatotoxicity. Apium graveolens (celery) has potential benefits in many disorders. Aim of the study: To investigate the possible protective role of celery seeds extract (CSE) against the damaging effects of CP on the liver. Materials and Methods: The study has included five groups of rats divided into, Group 1 as normal control, group 2 received a single dose (7mg/kg, i.p.) of CP, groups 3, and 4 pretreated daily with CSE in two different doses; group 3 (250mg/kg) and group 4 (500 mg/kg), and group 5 pretreated daily with the hepato-protective agent silymarin (100mg/kg) for 17 days followed by CP injection. Results: CP markedly affected the liver function. The glutathione peroxidase, and interleukin-6 levels were significantly decreased while the levels of heme oxygenase-1, interleukin-1 alpha were markedly elevated in the tissue homogenates of CP injected rats. The administration of CSE has ameliorated all the altered biochemical and histopathological changes of the liver; the antioxidant enzyme glutathione peroxidase, IL-6 level, and prostaglandin E2 were markedly increased while IL-1 alpha was reduced. Conclusion: CSE has a protective effect against acute hepatotoxicity induced by CP administration in rats that could be through inhibiting inflammation and increasing antioxidant activities.