Cd Transport Research Articles

Strategic Selenium Application Methods and Timing Enhance Grain Yield, Minimize Cadmium Bioaccumulation, and Optimize Selenium Fortification in Triticum aestivum L.

Based on previous research, we hypothesized that an SeVI and SeMet combined application at different growth stages could increase the yield and Se concentration and decrease Cd concentration in wheat grains. To verify this hypothesis, we designed a pot experiment throughout the wheat growth period and investigated the effects of SeIV, SeVI, and SeMet applied individually or in combination at different growth stages on yield traits; Cd absorption and transport; and Se content under Cd stress. The results indicated that grain yield was the highest under the SeVI individual application treatment and the SeVI (at the seedling, jointing, and heading stages) and SeMet (at the filling stage) combined application treatment (3 + 1 treatment), showing a more than 42% increase compared with the Cd-only control treatment (CK). Under the 5 mg/kg Cd stress, the grain Cd content in the 3 + 1 treatment was 34.1% lower than that in CK and over 14.1% lower than those in Se individual treatments. Furthermore, grain Se content was the highest under the 3 + 1 treatment, being 160.8%, 99.7%, and 39.5% higher than those in the SeIV, SeVI, and SeMet individual treatments under 5 mg/kg Cd stress. This may be attributed to early SeVI application in the 3 + 1 treatment, which enhanced yield traits and effectively promoted the retention of Cd in the middle and lower organs, reducing its transport to the grains. Furthermore, the SeMet application enhanced Se translocation to the grains, further reducing Cd content and increasing the Se concentration. In conclusion, the combined application of SeVI (at the seedling, jointing, and heading stages) and SeMet (at the filling stage) helped achieve the desired outcomes of high grain yield, low Cd content, and Se enrichment under Cd stress.

Cadmium translocation combined with metabolomics analysis revealed potential mechanisms of MT@MSN-CS and GSH@MSN-CS in reducing cadmium accumulation in rice (Oryza sativa L.) grains.

Applying nano-delivery systems for phytohormones via foliar application has proven effective in reducing grain cadmium (Cd) levels in crops. However, the mechanisms underlying this reduction remain inadequately understood. This study integrated the determination of leaf photosynthetic parameters, Cd translocation analysis, and metabolomics to elucidate the effects of reduced glutathione (GSH) and melatonin (MT), delivered with or without chitosan-encapsulated mesoporous silica nanoparticles (MSN-CS), on grain Cd levels in rice. Our findings revealed that the foliar application of MT@MSN-CS significantly outperformed MT alone in reducing grain Cd levels and enhancing leaf photosynthesis under Cd stress. Conversely, GSH@MSN-CS showed comparable effects to GSH alone. Foliar-applied GSH@MSN-CS and MT@MSN-CS both decreased the Cd transport coefficients from panicle nodes to brown rice by 26.2-53.3%, with MT@MSN-CS demonstrating superior efficiency in reducing Cd concentrations across roots, stems, leaves, panicle nodes, and grains. Metabolomic analysis revealed substantial shifts in rice metabolite profiles following GSH@MSN-CS and MT@MSN-CS treatments. Foliar application of MT@MSN-CS or GSH@MSN-CS may rapidly and effectively activate the primary antioxidant defense system and alleviate membrane lipid peroxidation in rice grown on low-to-moderately Cd-contaminated soils by upregulating amino acid metabolism. The secondary defense mechanism, phenylpropanoid biosynthesis, was reprogrammed to reduce energy expenditure and decrease Cd translocation.

Lower Cadmium Bioavailability and Toxicity in Japonica Rice than in Indica Rice: Mechanisms and Health Implications.

Cadmium (Cd) is efficiently transferred from soil to food crops, notably rice. Research indicates that indica rice grains may accumulate more Cd than japonica cultivars; however, differences in Cd bioavailability (the fraction of ingested rice Cd absorbed into the systemic circulation) and toxicity remain unexplored, thus hindering a comprehensive understanding of exposure and health risks. To address this, a mouse bioassay was conducted to evaluate the relative bioavailability (RBA) of Cd in 35 samples each of japonica and indica rice, determining which type exhibits lower Cd bioavailability. The results revealed a significantly lower mean Cd-RBA in japonica rice (49.6 ± 7.8%) compared to indica rice (65.6 ± 12.2%). This disparity is attributed primarily to the 1.25- and 1.37-fold higher concentrations of calcium (Ca) and iron (Fe) in japonica rice, which enhanced Ca and Fe absorption from the intestine and reduced duodenal expression of Ca and Fe transporters by 1.8-5.9 times in mice consuming japonica rice, thereby decreasing Cd transcellular transport. Additionally, japonica rice consumption promoted beneficial gut probiotics (Bifidobacterium pseudolongum and Lactobacillus reuteri) and metabolites, particularly short-chain fatty acids and peptides, potentially increasing mineral absorption and reducing Cd uptake. Moreover, mice fed japonica rice exhibited 1.35-1.47 times higher gene expression of intestinal tight junctions, enhancing intestinal barrier function and reducing extracellular Cd transport. Consequently, consuming Cd-containing japonica rice was associated with lower oxidative stress, inflammation, and cancer risks in mice compared to indica rice consumption. This study significantly enhances our understanding on the health risks associated with Cd in different rice subspecies.

Sustainable remediation of Cd-contaminated farmland through the rotation of rapeseed–rice varieties with different Cd accumulation potentials

In response to the safety risks posed by cadmium (Cd)-contaminated rice fields worldwide, a suitable production-and-restoration strategy is required for actual agricultural practices. To investigate the remediation effects of different accumulation varieties in rapeseed−rice cropping systems and their influence on Cd migration and transportation, field experiments were conducted based on different planting combinations (FWHR, conventional rice variety (HR) monoculture under fallow; FWLR, low Cd-accumulating rice variety (LR) monoculture under fallow; LOLR, LO (low Cd-accumulating rapeseed variety)-LR rotation; LOHR, LO-HR rotation; HOLR, HO (high Cd-accumulating rapeseed variety)-LR rotation; HOHR, HO-HR rotation). The study found that a rapeseed and rice rotation with appropriate varieties could reduce the rice grain Cd content, increase rice yield, and remove soil Cd without affecting agricultural production efficiency. Compared to the fallow-conventional rice pattern, various rapeseed−rice rotations reduced the Cd content of rice grains by 15%–38%, and significantly increased the available potassium (Ava-K) in the subsequent rice soil by 29.6–56.4mg/kg. The total economic benefits increased by $500–$1800 per hectare. A high accumulation variety of rapeseed and low accumulation variety of rice produced the most effective reduction in Cd levels, with a reduction rate of 38% in brown rice and an annual removal rate of 24.42g/hm2. This combination also resulted in a 29% increase in rice yield compared to the fallow-low accumulation variety rice pattern. Structural equation modeling revealed that with the combined action of crop rotation and variety selection the crop rotation directly reduced the soil available Cd or had an indirect effect by weakening the root-zone acidification effect and increasing soil Ava-P. The rotation of rapeseed and rice with carefully selected matching varieties is a feasible solution for the safe production and pollution remediation of Cd-contaminated paddy fields.

Effect of Phosphate Amendment on Cadmium Accumulation in Pepper (Capsicum annuum L.) Grown in Geogenic Cd-Rich Soil from the Karst Region

Soil in the karst region usually features high geogenic cadmium (Cd) and limited available phosphorus (P). Appropriate phosphate amendment is crucial for alleviating Cd accumulation in food crops and reducing health risks. However, the interaction of Cd and P in geogenic Cd-rich soil-plant systems is poorly understood. In this study, a pot experiment was conducted to investigate the translocation of Cd in the soil-pepper system under different amendment rates of Ca(H2PO4)2. The results showed that the biomass of pepper was not affected by the application rates of Ca(H2PO4)2, even up to 0.45 g/kg, but was affected by the application of nitrogen and potassium fertilizers. High contents of total Cd (6.19 mg/kg) and bioavailable Cd (2.72 mg/kg, 44%) in the studied soils resulted in elevated Cd content in pepper, and it decreased in the order of root (8.18 mg/kg) > stem (4.89 mg/kg) > fruit (3.88 mg/kg). This indicates that pepper planted in the studied soils may present potential health risks. Furthermore, phosphate amendment neither influences the bioavailable Cd in rhizosphere soil nor Cd uptake and transport in pepper plants. The findings of this study highlight that monocalcium phosphate is not a suitable choice for reducing the accumulation of Cd in pepper fruits in the studied soil and that other remediation strategies are needed.

Exogenous salicylic acid reduces cadmium content in spinach (Spinacia oleracea L.) shoots under cadmium stress

BackgroundConsumption of leafy vegetables is a primary route of cadmium (Cd) exposure in the human body. Salicylic acid (SA) is a major stress signaling molecule that alleviates Cd toxicity in various plants. Our study aimed to investigate the effects of different SA concentrations on spinach growth, cadmium accumulation, and stress resistance physiology under cadmium stress (50 µmol/L).ResultsCd stress significantly markedly decreased spinach growth and biomass, reduced its photosynthetic efficiency, increased activities of antioxidative enzymes, and upregulated the relative expression of several genes involved in cadmium absorption and transport compared to the control. The exogenous application of SA mitigated the harmful effects of Cd in spinach. 0.8 and 1.6 mmol/L SA significantly increased spinach root length, plant height, and biomass and decreased the Cd content in shoots by 30.03 and 17.35% compared to the Cd-treated group. Moreover, SA alleviated the yellowing of leaves caused by Cd stress. Exogenous SA ameliorated Cd toxicity in spinach by reducing reactive oxygen species, malondialdehyde, proline, and soluble protein levels. Exogenous SA application reduced Cd absorption in spinach leaves by downregulating the expression of genes involved in Cd transport, such as SoHMA4-like, SoNramp3.1-like, SoNramp6-like, and SoNramp7.2-like. Principal component analysis and correlation analysis showed that exogenous SA application under Cd stress was correlated with plant Cd content, photosynthetic pigment content, and relative expression of Cd absorption and transportation-related genes.ConclusionsTo summarize, these findings indicate that SA mitigates Cd toxicity in spinach by reversing the adverse effects of Cd stress on plant growth and reducing Cd accumulation in the shoots.

Promoters, Key Cis-Regulatory Elements, and Their Potential Applications in Regulation of Cadmium (Cd) in Rice.

Rice (Oryza sativa), a globally significant staple crop, is crucial for ensuring human food security due to its high yield and quality. However, the intensification of industrial activities has resulted in escalating cadmium (Cd) pollution in agricultural soils, posing a substantial threat to rice production. To address this challenge, this review comprehensively analyzes rice promoters, with a particular focus on identifying and characterizing key cis-regulatory elements (CREs) within them. By elucidating the roles of these CREs in regulating Cd stress response and accumulation in rice, we aim to establish a scientific foundation for developing rice varieties with reduced Cd accumulation and enhanced tolerance. Furthermore, based on the current understanding of plant promoters and their associated CREs, our study identifies several critical research directions. These include the exploration of tissue-specific and inducible promoters, as well as the discovery of novel CREs specifically involved in the mechanisms of Cd uptake, transport, and detoxification in rice. Our findings not only contribute to the existing knowledge base on genetic engineering strategies for mitigating Cd contamination in rice but pave the way for future research aimed at enhancing rice's resilience to Cd pollution, ultimately contributing to the safeguarding of global food security.

Screening High-Biomass Grasses for Cadmium Phytoremediation.

Investigating the ability of non-hyperaccumulator plants to grow in soils polluted by cadmium (Cd) and their potential for phytostabilization or phytoextraction is essential for assessing their use in phytomanagement efficiency. Therefore, we evaluated the tolerance of high-biomass grasses to Cd by measuring biomass production and element accumulation and valued them for their suitability for phytoextraction or phytostabilization purposes on moderately Cd-polluted land (total Cd concentration of 7.5 mg kg-1) by determining Cd accumulation in the plants and calculating the bioconcentration (Cd BCF) and translocation factors (Cd TF). Among the ten species under investigation, Panicum maximum cv. Massai and Pennisetum glaucum cv. Purpureum Schum showed lower root biomass due to Cd exposure. Cadmium exposure altered element accumulation in some grass species by reducing P, K, and Mg accumulation in P. glaucum cv. Purpureum Schum; K accumulation in P. maximum cv. Massai; Mg accumulation in P. maximum cv. Mombaça; Ca, Fe, and Zn accumulation in P. maximum cv. Aruana; and B accumulation in Brachiaria brizantha cv. Piatã. However, this was not correlated with lowered biomass production, except for K, which was associated with lowered root biomass allocation in P. maximum cv. Massai and P. glaucum cv. Purpureum Schum. Cadmium concentrations decreased from roots to shoots, indicating a clear limitation of upward Cd transport. Although some grasses exhibited a Cd BCF > 1, the Cd TF remained below 0.4 for all tested species. These results indicate that, under moderate Cd pollution, the evaluated grasses are more suitable for Cd phytostabilization than phytoextraction, except for P. maximum cv. Massai and P. glaucum cv. Purpureum Schum, which showed inhibited root growth and may not be efficient over time.

Interactional Effects of Soil and Foliar Application of Manganese Combined with Soil Stabilizer on Cd Accumulation in Rice

Crucial environmental and health problems occur in acidic paddy fields contaminated by cadmium （Cd）. Alkaline stabilizers and manganese （Mn） fertilizers are often applied to soil to reduce Cd pollution in rice. However, the bioavailability of Mn may also be affected by stabilizers and cannot effectively reduce Cd accumulation in rice. In this study, an alkaline compound stabilizer containing organic matter and silica-calcium was used in combination with a soil application of Mn antagonist （Mn-sulfate）, and organic Mn-fertilizer （EDTA-Mn, amino acid-bound Mn） was sprayed on the leaves of rice at the booting stage and filling stage to elucidate the effectiveness and mechanism of the combined treatments. In the pot experiment, three types of treatments were selected： soil application of stabilizer/Mn fertilizer （T）, Mn foliar spray （Y）, and the combination of soil stabilizer/Mn fertilizer and Mn foliar spray （T+Y）, and a total of 12 treatments were performed. In the field experiment, two types of treatments were selected： Mn foliar spray （Y） and the combination of soil stabilizer/Mn fertilizer and Mn foliar spray （T+Y）, and nine treatments were performed in total. The pot experiment results showed that, compared with that in CK, the Cd content in brown rice treated with the single applications of stabilizer, Mn-sulfate, and 1/2 stabilizer+1/2 Mn-sulfate were decreased by 19.1%, 44.0%, and 51.9%, respectively. On the basis of 1/2 stabilizer+1/2 Mn-sulfate, the Mn foliar spray of EDTA-Mn and amino acid-bound Mn further reduced Cd in brown rice by 46.1% and 46.9%, respectively. Field experiment results showed that, compared with that in CK, Mn foliar spray with EDTA-Mn and amino acid-bound Mn combined with 1/2 stabilizer + 1/2 Mn-sulfate reduced Cd in brown rice by 86.9% and 83.6%, respectively, and the Cd bioconcentration factor were both reduced to 0.05. A single application of stabilizer significantly increased the pH of the soil in the pot experiment and reduced the soil CaCl2-extractable Cd. However, the single application of stabilizer significantly inhibited the formation of Mn plaque on the root surface, while 1/2 stabilizer+1/2 Mn sulfate significantly increased the iron-Mn plaque on the root surface, and more Cd was also fixed on root surface. The addition of Mn foliar spray significantly reduced Cd transport from the stem and leaves to the rice grains. The field experiment showed that the 1/2 stabilizer+1/2 Mn-sulfate combined with Mn foliar spray significantly reduced Cd transport from the stem and leaves to the rice grains, though this combined treatment did not increase soil pH and reduce CaCl2-Cd as much as the treatment of the full dose of stabilizer. In summary, pot and field experiments consistently showed that 1/2 stabilizer+1/2 Mn-sulfate combined with organic Mn foliar spray could inhibit the uptake and transport of Cd in whole soil-plant systems and effectively reduce the Cd accumulation in brown rice.

Safe Utilization Effect of Passivator, Foliar Inhibitor, and Their Combined Application on Cadmium-contaminated Farmland

This study sought to explore the effects of passivator, leaf inhibitor, and their combination on cadmium （Cd）-contaminated farmland and to realize the safe utilization of cultivated land. Coconut shell biochar and calcium magnesium phosphate fertilizer were selected as soil passivators and foliar selenium fertilizer was used as a foliar inhibitor. The best combination was selected through a wheat-maize rotation experiment. The results showed that： ① The soil pH, organic matter content, and CEC could be improved to varying degrees by the application of passivators. ② The grain yield of wheat and maize increased to different degrees after the application of the passivator and foliar inhibitor. ③ The BFL treatment （biochar 1 800 kg·hm-2 +calcium magnesium phosphate 600 kg hm-2 + foliar inhibitor 3 L·hm-2） during the maize season had the best effect, which was significantly lower than that in CK by 13.85%. The effect of the BF treatment （biochar 1 800 kg·hm-2 + calcium magnesium phosphate 600 kg·hm-2） in wheat season was the best, which was significantly reduced by 11.21% compared with that in CK. ④ The BF treatment （biochar 1 800 kg·hm-2 + calcium magnesium phosphate 600 kg·hm-2） had the best effect on reducing Cd content in roots, straws, and grains of maize and wheat, reducing Cd content in roots of wheat and maize by 20.55% and 27.81%, Cd content in straws by 11.39% and 13.70%, and Cd content in grains by 48.97% and 50.44%, respectively. Therefore, considering all the indexes, the combination of passivators and foliar inhibitor, especially biochar combined with calcium magnesium phosphate fertilizer and biochar and calcium magnesium phosphate fertilizer combined with foliar inhibitor, could more effectively reduce the absorption, transport, and enrichment of Cd in maize and wheat and could be popularized and applied in safe use cultivated land.

Coupling a process-driven model with geographic information system enables the spatial predication of cadmium in paddy soils

Policies on the management of paddy fields are usually made at a broad scale and from a long-term perspective, while predicting the spatial extent of cadmium (Cd) contamination in paddy soils remains challenging. In this study, we developed a process-driven spatial model to quantify the transport of Cd in paddy soils and validated it against observed data from a 10-year regional investigation in southern China. Using a geographic information system and Monte Carlo simulation, the model was then applied to evaluate the effectiveness of different remediation strategies for contaminated paddy fields at field-to-regional scales in a 100-year period. In the last decade, atmospheric emissions have accounted for 43.5 % of the total Cd input in local paddy soils. However, the local clean air act failed to mitigate Cd contamination in 99.8 % of study area over the period of 2020–2120 because straw return became the dominant contributor to Cd inputs. Improving aerosol emission reductions by 3 % per year, stopping straw return to soil, and cleaning irrigation channels would take approximately 30 years (2020–2050) to protect 95 % of local rice production from causing an excessive human Cd kidney burden, especially in the paddy fields located in mining-affected areas.

Effect of coexisting nutrient divalent cations on cadmium transport in soil-herbal crop systems

Cadmium (Cd) pollution in Chinese herbal medicines poses a serious risk to medication safety. Regulating Cd uptake, transport, and accumulation in plants through ion-ion interactions offers a novel, environmentally sustainable, and practical approach to address this issue. However, the effects and underlying mechanisms of coexisting divalent cations zinc (Zn), magnesium (Mg), and manganese (Mn) on Cd uptake by Ligusticum sinense cv. Chuanxiong (L. chuanxiong) have not been comprehensively studied or well understood. In this study, the application of coexisting these cations (Zn, Mg, Mn) could significantly promote the growth of L. chuanxiong (21.11%–36.04%) and change the mobility of Cd in the soil-crop system. Specifically, adding Zn decreased Cd content in soil and plants by 18.23% and 20.62%, respectively, while Mg increased it by 10.99% and 62.27%. Mn addition, however, had no significant effect. Similar trends in soil enzyme activity were also observed with Zn, Mg, and Mn treatments. Simultaneously, the findings explore how coexisting divalent cations influence plant physiological responses, including photosynthesis and antioxidant capacities, enabling L. chuanxiong to better manage Cd stress. This study underscores the potential of ion-to-ion interactions as an effective approach to mitigate Cd accumulation, offering a practical and sustainable solution for enhancing the safety of Chinese herbal medicines. Additionally, the effects of mixed cation applications on Cd dynamics are complex, shaped by interactions between ion types, dosages, and their specific properties. These insights provide a foundation for developing more effective remediation strategies for Cd-contaminated soils, particularly in the cultivation of medicinal plants.

Effects of EDTANa2Fe on phytoavailability of cadmium and arsenic to rice (Oryza sativa L.)

Cadmium (Cd) and arsenic (As) that accumulate in rice grains can enter the human body via ingestion, posing a human health threat. Chelated iron (Fe) fertilizer application is an effective strategy for reducing Cd and As concentrations in grains; however, its mechanism of action is unknown. We investigated effects of ethylenediamine tetraacetic acid disodium ferrous (EDTA·Na2Fe) at Fe application rates of 0, 25, 50, and 75 mg kg−1 on Cd and As availability in soil and accumulation in rice grains. EDTA·Na2Fe significantly reduced soil CdAs availability and significantly decreased CaCl2Cd and KH2PO4As concentrations by 27.8–39.2 % and 17.7–28.4 %, respectively. EDTA·Na2Fe facilitated Fe plaque (IP) formation and increased Cd (CdIP) and As (AsIP) sequestration in IP; furthermore, FeIP, CdIP, and AsIP increased significantly by 70.7–125 %, 109–150 %, and 88.1–168 %, respectively. In roots, EDTA·Na2Fe reduced the Cd concentration (CdR) but increased the As concentration (AsR). EDTA·Na2Fe reduced the Cd (CdG) and As (AsG) concentrations in grains by 29.8–46.2 % and 18.5–33.3 %, respectively. The optimal simultaneous reduction effect of CdG and AsG was observed at an EDTA·Na2Fe application rate of 50 mg kg−1 Fe. The results indicated that CdG was mainly affected by Cd availability, translocation factor (TF) CdR/CdIP, and TF CdG/CdR, and AsG was mainly affected by TF AsG/AsR, followed by TF AsR/AsIP and AsIP. In summary, EDTA·Na2Fe reduced CdG and AsG by reducing Cd and As availability in soil, improving Cd and As sequestration in IP, and reducing Cd transport from IP to roots and As transport from roots to grain. Moderate application of EDTA·Na2Fe effectively reduced CdG and AsG in CdAs-contaminated paddy soil.

Arbuscular Mycorrhizal Fungi-Assisted Phytoremediation: A Promising Strategy for Cadmium-Contaminated Soils.

Arbuscular mycorrhizal fungi (AMF) have been shown to play a major role in regulating the accumulation, transport, and toxicity of cadmium (Cd) in plant tissues. This review aims to highlight the current understanding of the mechanisms by which AMF alleviate Cd toxicity in plants. Cd accumulation in agricultural soils has become an increasing global concern due to industrial activities and the use of phosphatic fertilizers. Cd toxicity disrupts various physiological processes in plants, adversely affecting growth, photosynthesis, oxidative stress responses, and secondary metabolism. AMF alleviate Cd stress in plants through multiple mechanisms, including reduced Cd transport into plant roots, improved plant nutritional status, modulation of organic acid and protein exudation, enhanced antioxidant capacity, and maintenance of ion homeostasis. AMF colonization also influences Cd speciation, bioavailability, and compartmentalization within plant tissues. The expression of metal transporter genes, as well as the synthesis of phytochelatins and metallothioneins, are modulated by AMF during Cd stress. However, the efficacy of AMF in mitigating Cd toxicity depends on several factors, such as soil properties, plant species, AMF taxa, and experimental duration. Further knowledge of the intricate plant-AMF-Cd interactions is crucial for optimizing AMF-assisted phytoremediation strategies and developing Cd-tolerant and high-yielding crop varieties for cultivation in contaminated soils.

Ethylene-mediated root endodermal barrier development in impeding Cd radial transport and accumulation in rice (Oryza sativa L.)

Ethylene plays crucial roles in the adaptation to cadmium (Cd) stress. Nevertheless, the impact of endogenous ethylene on radial transport of Cd in different rice cultivars are insufficiently understood. Herein, we investigated how ethylene involved in the formation of endodermal barriers in roots of Nipponbare with low-Cd accumulation and IR32307 with high-Cd accumulation ability and further assessed its influence on Cd radial transport. Our analysis indicated that both Cd stress and external ACC (1-aminocyclopropane-1-carboxylic acid, ethylene biosynthesis precursor) promoted the ethylene production. Intriguingly, the positive response of ethylene signal to Cd was more intensive in roots of Nipponbare than that of IR32307. The increased endogenous ethylene in rice roots promoted development of casparian strips (CSs) and suberin lamellae (SL). Specifically, external addition of ACC decreased the percentage of the DTIP-CS/DTIP-SL to root length by 44.4–79.6%/49.3–11.4% in Nipponbare and 18.7–19.9%/10.7–35.3% in IR32307, individually. The intrinsic molecular mechanism was mainly due to changes in the genes expression levels related to CSs/SL biosynthesis. Simultaneously, the analyses of apoplastic tracer (Propidium Iodide, PI) and cell-to-cell tracer (Fluorescein Diacetate, FDA) confirmed that the ethylene-mediated endodermal barriers were functional, which were in accordance with the increased/reduced Cd transport in roots. Eventually, the results of transcriptome analysis further shed a comprehensive insight that ethylene constructed the endodermal barrier through phenylpropanoid and cutin, suberine and wax biosynthesis to reduce Cd radial transport in rice, which are beneficial for the breeding of rice with low-Cd accumulating capacity in the future.

RsNRAMP5, a major metal transporter, promotes cadmium influx and ROS accumulation in radish (Raphanus sativus L.)

Arable soil contamination with heavy metals (HMs) poses a great potential threat to vegetable crops and human health. Radish (Raphanus sativus L.), an economical and popular root vegetable crop, is easily absorbed HMs by its taproot. Although the Natural Resistance-Associated Macrophage Proteins (NRAMPs) were participated in transporting a number of HMs in plants, whether and how the NRAMP genes involved in cadmium (Cd) uptake and transport remains elusive in radish. In this study, a total of nine RsNRAMP gene members were identified, which were classified into three subgroups and dispersed on six radish chromosomes. Three RsNRAMPs (RsNRAMP3, RsNRAMP4 and RsNRAMP5) displayed high expression in the vascular cambium, and they exhibited obviously Cd-induced expression, among which the expression of RsNRAMP4 and RsNRAMP5 reached to the highest level at 24h. Moreover, the RsNRAMP5 was localized to the plasma membrane and its promoter activity was dramatically induced by Cd exposure. Heterologous expression analysis indicated that over-expression of RsNRAMP5 significantly promoted the uptake of Cd, lead (Pb), iron (Fe) and manganese (Mn) in yeast cells. In addition, the transient over-expression of RsNRAMP5 promoted Cd uptake and enhanced ROS (reactive oxygen species) accumulation in radish cotyledons. These findings would expedite unraveling the molecular mechanism underlying RsNRAMP5-mediated Cd uptake and transport in radish.

OsAMT1.1 knockout-induced decrease in cadmium absorption and accumulation by rice related to cadmium absorption-related gene downregulation

Cadmium (Cd) is a hazardous heavy metal that poses a serious risk to human health through the food chain, with rice being a significant vector because of its tendency to accumulate Cd. Nitrogen (N), an essential element for plant growth, also affects the Cd absorption and accumulation in crops. This study investigated the effects of N application on Cd absorption and accumulation in Cd-contaminated soils. Potting experiment showed that increasing N concentrations significantly increased the plant biomass and Cd contents in rice tissues. Ammonium (NH4+) transporter gene OsAMT1.1 knockout led to a substantial reduction in Cd absorption and accumulation in all rice tissues compared to that in the wild-type plants. Specifically, osamt1.1 mutants increased the Cd content in culm tissues, whereas it was reduced in brown rice. In addition, OsAMT1.1 knockout reduced Cd2+ influx in roots under NH4+-N addition, although OsAMT1.1 lacked Cd transport ability when expressed in yeast. Gene expression analysis revealed that OsAMT1.1 knockout reduced Cd absorption-related genes (OsIRT1, OsNRAMP1, and OsNRAMP5) expression levels. These finding highlight the critical role of N supply and OsAMT1.1 in regulating the Cd content in rice, offering insights into the molecular mechanisms of Cd transportation in plants.

Regulatory effects and mechanism of different selenium species on cadmium accumulation in Triticum aestivum L. (microbial response, gene expression and element accumulation)

Decreasing cadmium (Cd) accumulation in wheat grain is significant for human health. This study compared the effect of soil applied different selenium (Se) species on Cd accumulation in wheat. In Cd–contaminated soil, the applications of inorganic Se species, organic Se species, and selenium nanoparticles (SeNPs) had little effect on physicochemical properties, available Cd content, and Cd fractions in soil, but changed the β diversity and relative abundance of bacteria at genus level. The application of different Se species reduced Cd concentration in wheat grain, and inorganic Se species had the best effect on reducing Cd, with a decrease of 24.1%–57.0%. Under Cd stress, selenite (Se(IV)) application inhibited the expression of Cd transport–related genes; selenate (Se(VI)) application mainly prompted the expression of gene associated with Cd sequestration in vacuole; and seleno–L–methionine (SeMet) and SeNPs application inhibited the expression of genes related to Cd uptake and transport. Further, Se application alleviated the toxicity of Cd stress on chloroplast. Correlation and stepwise regression equation indicated that different Se species had different crucial part affecting grain Cd accumulation. When Cd was absorbed by plant root, different Se species differentially affected the synergistic effect between nutrient elements and Cd. In all, Se application, especially Se(IV) can be effectively used to reduce grain Cd accumulation.

Application of a new TOPO-Cuprone type V deep eutectic solvent as a carrier of Zn(II), Cd(II), Pb(II) and Cu(II) ions in polymer inclusion membranes. Chemometric methods for new hydrogen bond acceptor (HBA) selection

A new hydrogen bond donor was selected as a deep eutectic solvent (DES) component using 0D, 1D, and 2D molecular descriptors and chemometric methods such as hierarchical cluster analysis (HCA) and principal component analysis (PCA). Compared to typical substances used as hydrogen bond donors (HBDs), namely carboxylic acids, it was found that α-benzoin oxime (Cuprone), despite of its different properties, is also a suitable DES constituent. A new DES was synthesized and applied as a carrier of Zn(II), Cd(II), Pb(II), and Cu(II) ions in polymer inclusion membranes (PIMs). Tri n-octyl phosphine oxide (TOPO) was applied as a hydrogen bond acceptor (HBA), and Cuprone was used as the HBD in the 2:1 M ratio, respectively. The transport properties of a new DES were compared with PIM systems in which TOPO:benzoic acid, TOPO:dodecanoic acid, and pure TOPO were applied. The selected physicochemical properties such as density, viscosity, and thermal stability of the new DES were examined. The transport and separation of Cd(II), Zn(II), Pb(II), and Cu(II) ions from chloride media using a new DES as a carrier were investigated. The influence of the carrier content and the presence of a plasticizer on transport efficiency during long-lasting experiments were also examined.It was found that the observed membrane transport selectivity order Zn(II) > Cd(II) > Pb(II)≫Cu(II) results from the presence of TOPO in the newly prepared DES. Moreover, systems with a plasticizer exhibit high operational stability during long–term experiments lasting over 500 h. Some drawbacks which appeared during the transport experiments were noticed and described in detail.

Synthetic communities derived from the core endophytic microbiome of hyperaccumulators and their role in cadmium phytoremediation

BackgroundAlthough numerous endophytic bacteria have been isolated and characterized from cadmium (Cd) hyperaccumulators, the contribution and potential application of the core endophytic microbiomes on facilitating phytoremediation were still lack of intensive recognition. Therefore, a 2-year field sampling in different location were firstly conducted to identify the unique core microbiome in Cd hyperaccumulators, among which the representative cultivable bacteria of different genera were then selected to construct synthetic communities (SynComs). Finally, the effects and mechanisms of the optimized SynCom in regulating Cd accumulation in different ecotypes of Sedum alfredii were studied to declare the potential application of the bacterial agents based on core microbiome.ResultsThrough an innovative network analysis workflow, 97 core bacterial taxa unique to hyperaccumulator Sedum was identified based on a 2-year field 16S rRNA sequencing data. A SynCom comprising 13 selected strains belonging to 6 different genera was then constructed. Under the combined selection pressure of the plant and Cd contamination, Alcaligenes sp. exhibited antagonistic relationships with other genera and plant Cd concentration. Five representative strains of the other five genera were further conducted genome resequencing and developed six SynComs, whose effects on Cd phytoremediation were compared with single strains by hydroponic experiments. The results showed that SynCom-NS comprising four strains (including Leifsonia shinshuensis, Novosphingobium lindaniclasticum, Ochrobactrum anthropi, and Pseudomonas izuensis) had the greatest potential to enhance Cd phytoremediation. After inoculation with SynCom-NS, genes related to Cd transport, antioxidative defense, and phytohormone signaling pathways were significantly upregulated in both ecotypes of S. alfredii, so as to promote plant growth, Cd uptake, and translocation.ConclusionIn this study, we designed an innovative network analysis workflow to identify the core endophytic microbiome in hyperaccumulator. Based on the cultivable core bacteria, an optimized SynCom-NS was constructed and verified to have great potential in enhancing phytoremediation. This work not only provided a framework for identifying core microbiomes associated with specific features but also paved the way for the construction of functional synthetic communities derived from core microbiomes to develop high efficient agricultural agents.5KavnCJpVp3k2g6uQCMsTeVideo