In this study, a jet cavitation device aimed at producing two-dimensional nanosheets was designed. The effects of cavitation generator type and jet pressure on the cavitation inception and intensity were examined by monitoring the changes of sound pressure level (ΔSPL). As such, the optimized cavitation generator with the best cavitation capability under the same ambient condition was determined. Further, BN and MoS2, two kinds of layered materials, were exfoliated into individual flakes in aqueous solutions by this jet cavitation device. By investigating the morphology of these exfoliated flakes via scanning electron microscopy and transmission electron microscope, it was found that these pristine materials were mostly exfoliated into two-dimensional nanosheets, among which even monolayers were generally presented. This exfoliation process happened mainly due to the cavitation-induced intensive tensile stress acting on the layered materials. As graphene has been produced by this device successfully, it is anticipated that this jet cavitation device is suitable for producing other various two-dimensional nanosheets.