Resting energy expenditure (REE), adjusted for total lean mass (LM), is lower in African American (AA) than Caucasian American (CA) children. Some adult studies suggest that AA-CA differences in lean mass compartments explain this REE difference. Similar data are limited in children. To evaluate differences in compartment-specific lean mass between AA and CA children and examine the individual contributions of high-metabolic rate-at-rest trunk lean mass (TrLM) and low-metabolic-rate-at-rest appendicular lean mass (AppLM) for AA-CA differences in REE. We studied a convenience sample of 594 AA (n=281) and CA (n=313) children. REE was measured by using indirect calorimetry; dual-energy X-ray absorptiometry was used to assess body composition. ANCOVAs were performed to examine AA-CA differences in TrLM, AppLM and REE. After accounting for age, sex, height, pubertal development, bone mass and adiposity, REE was evaluated adjusting for total LM (model A) and separately adjusting for TrLM and AppLM (model B). African American children had greater adjusted AppLM (17.8±0.2 [SE] vs. 16.0±0.2kg, p<0.001) and lower TrLM (17.2±0.2 vs. 17.7±0.2kg, p=0.022) than CA children. REE adjusted for total LM was 77±16kcal/d lower in AA than CA (p<0.001). However, after accounting separately for AppLM and TrLM, the discrepancy in REE between the groups declined to 28±19kcal/d (p=0.14). In the adjusted model, both TrLM (p<0.001) and AppLM (p<0.027) were independently associated with REE. In children, AA-CA differences in REE appear mostly attributable to differences in body composition. Lower REE in AA children is likely due to lower TrLM and greater AppLM.