Caspian Roach Research Articles

ЧИСЛЕННОСТЬ СЕГОЛЕТОК ВОБЛЫ, ЛЕЩА И СУДАКА В ЗАПАДНОЙ ЧАСТИ СЕВЕРНОГО КАСПИЯ В 2012-2016 ГОДАХ

The article presents the data obtained in the result of scientific research expeditions aiming to determine abundance of roach ( Rutilus caspicus ), bream ( Abramis brama ) and pike-perch ( Sander lucioperca ) in the water area of the Western part of the Northern Caspian Sea in September-October 2012-2016. Roach and bream fingerlings, that are the largest part of anadromous fishes of the Volga-Caspian fishery basin, predominate in catches of minnow trawls. Theirs total share is 85.9-99.6%. Weight density of pike-perch is not so great - 0.2-2.0%. Level of yield of roach, bream, pike-perch fingerlings in the Northern Caspian Sea varies from year to year (roach 4.5-7 mln, bream 4.0-12.9 mln, pike-perch 0.030-0.163 mln specimens) and determined by abiotic and biotic complex of factors, the general of which is the water content of the Volga river in the flood-time period. The number of young fishes increases in the period with high level of flowing, in low flowing times it decreases. The most favorable conditions for fish reproduction were in high-water periods in 2013 and 2016, and least favorable - in extremely law water periods in 2015. The maximum number of bream fingerlings reached in 2013, roach - in 2012 and 2013, bream - in 2016. The minimum number of all three fish species was noted in 2015. By the results of researches of bream breed, years 2012, 2013 and 2016 are considered not so great yielding years; years 2014 and 2015 are low-yield years. The breed of roach and bream from 2012 to 2016 are estimated like low yield years, which was determined not only due to low water level of the Volga river, but because of a decrease of the population number of these fish species and breeding population on breeding grounds.

Sublethal effects of copper nanoparticles on the histology of gill, liver and kidney of the Caspian roach, Rutilus rutilus caspicus

The current study has determined the toxicity effects of copper nanoparticles on the some vital organs such as gill, liver and kidney of Caspian Roach; Rutillus rutillus caspicus. For this purpose, 120 fishes were used as experimental samples and exposed to 0.1, 0.2 and 0.5 mg/L of Cu nanoparticles for 21 days, and 30 fishes assumed as the experiment control. The mean water temperature of the aquaria was 22±2 oC, dissolved oxygen 5.2 mg/L, pH at 7±0.004 and the concentration of calcium carbonate was 270 ppm. On 7, 14 and 21 days after exposing the fishes to copper nanoparticles, three fishes were randomly selected from each aquaria, sacrificed and samples from their gill, liver and kidney were taken and fixed in cold 10 % buffered formalin. Then microscopic sections were prepared and examined by light microscope which showed histological alternations in the gill, liver and kidney tissues. Evaluation of these changes could be useful in estimating the harmful effects of copper nanoparticles. Histological alternation in gills included: hyperplasia, fusion and detachment of secondary lamellae, blood congestion in vascular axis of primary filaments, reduced secondary lamellae length and cellular degeneration. Histological changes in liver included blood congestion in the central veins, cytoplasmic vacuolation of the hepatocytes, cellular degeneration and congestion in the blood sinusoids and necrosis of the hepatocytes. Histological changes in kidneys included glomerular shrinkage, severe degeneration in the tubules cells, interstitial tissue and glomerulus, increase in interstitial tissue cells and macrophages aggregation. The degree of damages was more intensive at higher copper nanoparticles concentrations. The result of the study showed that copper nanoparticles could cause severe damages in the vital tissues of Caspian roach; Rutillus rutillus caspicus and have lethal effects for fish.

Dietary administration of Pontogammarus maeoticus extract affects immune responses, stress resistance, feed intake and growth performance of caspian roach (Rutilus caspicus) fingerlings

Dietary administration of immunostimulants showed promising results for elevation of immune responses and disease resistance. The aim of the present study was to investigate the effects of dietary Pontogammarus (Pontogammarus maeoticus) extract on innate immune response, stress resistance, feed intake and growth performance of the Caspian roach fingerling. Two levels of P. maeoticus extract dilution with distilled water 1:25 [T1] and 1:50 [T2] were prepared. Experimental diets were prepared by top-spraying the basal diet with equal amount (2%) of diluted extracts. One hundred and eighty Caspian roach fingerlings (4.30 ± 0.10 g) were supplied, stocked in nine 100-L tanks (three treatment repeated in triplicates) and fed on experimental diets for 8 weeks. At the end of the trial, serum innate immune parameters (Total Ig, ACH50, and lysozyme activity), resistance against salinity stress, feed intake and growth parameters were measured. The results revealed remarkable increase of innate immune parameters and resistance against salinity stress in roach fed P. maeoticus extracts (P < 0.05). Also, growth performance and food intake were notably improved in P. maeoticus extracts fed fish (P < 0.05). These results revealed beneficial effects of P. maeoticus extract on innate immune response, resistance, feed intake as well as growth performance of the Caspian roach.

Effects of dietary retinol acetate on growth performance, skin mucus immune responses and haematological parameters of Caspian roach (Rutilus caspicus)

Three retinol acetate (vitamin A)-supplemented diets containing 0, 5,000, 10,000 IU kg−1 diet (in dry weight) were fed twice daily to triplicate groups (50 fish/group) of Caspian roach (Rutilus caspicus) (1.91 ± 0.07 g) for 40 days. At the end of feeding trial, skin mucus antimicrobial activity against Streptococcus faecium, Serratia marcescens, Staphylococcus aureus and Escherichia coli as well as soluble protein level, growth performance and haematological parameters was evaluated. Growth performance including final weight, weight gain, specific growth rate and food conservation ratio as well as the survival rate showed no significant differences between vitamin A-supplemented diets and control groups (p > .05). The results revealed that the administration of 10,000 IU vitamin A kg−1 significantly increased skin mucus antibacterial activity (p .05), except white blood cell counts that were significantly higher in vitamin A-fed roach (p < .05). The results of the present study indicate that dietary vitamin A can modulate mucosal immune response of Caspian roach.

Ovarian development of Caspian roach, Rutilus caspicus, in southern Caspian Sea: A histological and ultrastructural study

The histology and ultrastructure of the ovarian maturation process in Caspian roach, Rutilus caspicus , was studied. A total 170 female specimens were collected from the Gharasoo River, Bandar Turkmen, the southern Caspian Sea to evaluate its maturation cycle. Based on the results, its ovarian follicle’s development could classified into six stages by distinct characteristics. Minimum and maximum diameter of oocytes were recorded in the chromatin-nucleolus and maturation stages as 56.34±3.74 and 918.83±14.82 µm, respectively. The zona radiata was observed from the cortical alveoli stage and its maximum diameter measured in the secondary vitellogenesis stage as 93.11±23.0 µm. Gonadosomatic index (GSI) reached to its peak in mid-March and its sharp drop in the late April showed its spawning period from late March or early April till the end of April. A positive correlation was found between the GSI and HSI in the vitellogenesis stage. The results also revealed Caspian roach as iteroparous synchronous spawner.

Environmental impact of zinc on hematological, biochemical and immunological parameters in Caspian Roach (Rutilus caspicus).

The aim of study was to investigate the toxicity effect of zinc at low concentration (10% LC50) and high doses (50%LC50) on roach (Rutilus caspicus). Roach with an average weight of 3.3 g and lengths of 4 cm were used in this work. Acclimatized fish were fed daily with a formulated feed.Caspian Roach were exposed to a low concentration of 10% LC50 and high concentration of 50% LC50 ofzinc for a period of control (0), 24, 48 and 96h static toxicity examination, operated within the tank of 400L. In 10% LC50 concentration zinc (96h) immune parameters neutrophil, monocyte and lymphocyte are not shown significant change toward control group (P>0.05) and in 50% LC50 concentration zinc (96h), immune factors lym showed significant decrease toward control but neu factor significantly increase toward control group (P 0.05). In low and high concentrations of zinc, results showed zinc can cause changes in hematological, biochemical and immunological factors in Caspian roach; therefore, assessment of these indicators, could be useful to detect pollution of aquatic ecosystems.

Influence of the hydrological regime in the lower reaches of the Volga delta on natural fish reproduction

This article considers the influence of the hydrological regime in the lower reaches of the Volga delta (based on the example of the Damchiksky site of the Astrakhan Reserve) during high water periods in the low-water year of 2012 and medium-water year of 2013 on the abundance of producers entering for spawning and the density of aggregations of juveniles of roach Rutilus caspicus, vulgaris rudd Scardinius erythrophthalmus, and river perch Perca fluviatilis. It is shown that the fish mass spawning in 2013 was more effective and occurred on floodplain spawning areas with the sequential succession of the investigated species, while the fish spawning was observed in eriks and was less effective in 2012. It was revealed that vulgaris rudd shows the highest adaptation to insufficient watering conditions.

RETRACTED: Detection of LC50 of crude oil in silver carp (Hypophthalmicthys molitrix), common carp (Cyprinus carpio) and Caspian roach (Rutilus rutilus caspicus)

RETRACTED: Detection of LC₅₀ of crude oil in silver carp (<i>Hypophthalmicthys molitrix</i>), common carp (<i>Cyprinus carpio</i>) and Caspian roach (<i>Rutilus rutilus caspicus</i>)

RETRACTED: Effects of nanometer-sized silver materials on survival response of Caspian roach (Rutilus rutilus caspicus)

The aim of this study was to investigate the acute effects of nanosilver as potentially dangerous additives to assess the mortality effects of this nanotechnology product on one of the valuable and important species of Caspian Sea. We comparedthetoxicity ofdifferent sizedsilver nanoparticles(AgNPs)whicharebeingwidelyusedwith Iranian consumersduetoitsuniqueantimicrobialactivity.EcotoxicassessmentsofAgNPsfrom twoIraniancompaniesof nanotechnology (Nanocid1: average particle size of 18 nm and Nanosil1: average particle size of < 100 nm) and silver salt (AgSO4) were conducted on the Caspian roach (Rutilus rutilus caspicus). Fish were exposed to technical dosesofNanocid (0,0.01,0.1, 0.5,1, 2.5 and 5 ppm),nominal doses ofNanosil(0,0.2, 2,20, 50, 100and 200ppm) and technical doses of silver sulfate (0, 0.001, 0.01, 0.1, 0.5, 1 and 2 ppm). The median lethal concentration (LC50) wasdeterminedwithprobiteanalysis.Theestimated96-hLC50values(95%confidencelimits)forNanocid,Nanosil andsilversaltwere0.028(0.017-0.046), 41.799(32.314-52.139)and0.003(0.001-0.006)ppm,respectively.LC50 ofsilverinsilversaltwaslowerthanotherproducts.Thepresentstudydemonstratesthatnano-Agwasseentobe less toxic than dissolved Ag þ toCaspianroach.Basedontheresultsofthisstudy,itissuggestedthatindustrial and commercial applications of AgNPs should be more carefully and thoroughly assessed as to their potential toxic effects to the aquatic environment and Caspian roach populations.

Effect of Lethal Concentration of Commercial Gasoline on Caspian Roach (Rutilus caspicus)

In this study, the mortality effects of commercial gasoline at different tested concentrations were evaluated on the common roach (Rutilus caspicus) and LC50 values for each time period (24, 48, 72, and 96 hours) have been determined. Roach with an average weight of 3.1 ± 0.45 g and lengths of 4 ± 0.25 cm were used in this study. After transferring the 200 fish to the laboratory, they were kept in tanks of 100 liters for one week to adapt them to the experimental conditions. After the adaptation period, 100 fish were selected randomly and divided into 14 treatments (0, 2, 5, 10, 20, 40, 60, 80, 100, 200, 400, 600, 800, and 100 ppm commercial gasoline). The treatments were completed in triplicate. The results of this study show that the 96-hour LC50 of commercial gasoline is 600.2 ± 0.44 ppm and the maximum allowable concentration (MAC) is 60.02 mg/L. The study demonstrates the deadly effects of commercial gasoline on the Caspian roach. Spillage of diesel and gasoline fuels from transport tankers can enter rivers and eventually the marine ecosystem, and reach nursery and spawning areas where it can become a serious threat to fish survival.

Determination of metals (As, Cu, Fe, and Zn) in two fish species from the Miankaleh wetland

Abstract The objective of this study was to determine the concentrations As, Cu, Fe, and Zn in the livers, gills, and muscles of vobla or Caspian roach, Rutilus caspicus (Yakovlev), and bighead goby, Neogobius gorlap (Iljin), from the Miankaleh wetland. The results showed that metal accumulation in the liver tissues was higher than in the gills and muscles for all metals, and Fe concentration was higher than Zn, Cu, and As in all the tissues studied. The concentrations of As, Cu, Fe, and Zn in the livers, gills, and muscles of bighead goby were higher than in vobla tissues, except for concentrations of As in the muscles. Fe, Cu, and Zn concentrations in the muscles were below the maximum permissible limit of the WHO and the FAO for both species, but the concentration of As in the muscles of both species exceeded the permissible limit proposed by the FAO; thus, human consumption should be limited. The levels of the other metals (Fe, Cu and Zn) were completely safe.

Acute toxicity bioassay of the mercury chloride and copper Sulphate in Rutilus caspicus and Rutilus kutum

The purpose of the present study was to determine the acute toxicity (LC 50 ) of HgCl 2 and CuSO 4 in Caspian roach ( Rutilus caspicus ) and CuSO 4 in Caspian kutum ( Rutilus kutum ). The Caspian roach LC 50 values for HgCl 2 at 24, 48, 72, and 96-hrs of exposure, were 0.64, 0.61, 0.42, and 0.28 mg L -1 , respectively, and for CuSO 4 were 11.55, 5.08, 2.49, and 1.47 mg L -1 , respectively. The Caspian roach LC 50 values for CuSO 4 at 24, 48, 72, and 96-hrs of exposure, were 5.31, 4.17, 3.20, and 2.25 mg L -1 , respectively. The results of this study showed that the toxicity of HgCl 2 is higher than that of CuSO 4 for the studied species. The mortality decreased with time, and most of the deaths were occurred during the first 24 hrs.

Distribution and abundance of fish in the southwest of Caspian Sea coastal waters

This study was carried out in southwestern Caspian Sea coastal area to elucidate demersal bony fishes distribution and abundance. Twenty two species were found in shore line, which belong to 6 families. In deeper waters down to 7 meters only 5 species of Gobiidae and 1 species of Syngnathidae were identified. Two species Neogobius caspius and N. pallasi were the main components of demersal fishes; however in some regions Rutilus caspicus, Rutilus kutum and Liza spp. were dominated. Most places were dominantly occupied by Atherina boyeri, which had the highest abundance among the pelagic fishes. Conversely, the Ponticola gorlap, N. melanostomus and Proterorhinus nasalis showed the lowest abundances (less than 1 ind./100 m2). Benthophilus stellatus and P. nasali had the lowest niche overlaps with other species. Both of these two species and P. gorlap are suggested to be classified as endangered or vulnerable species based on their low abundance. The distribution of Syngnathus abaster depends on algae, while algae grow up into the stony construction of the coastal line. A negative insignificant correlation was observed between pelagic and demersal fish abundances. The commercial fisheries data of 76 beach seines in our study area during 2002–2013 was compared with our results on fish abundance and species composition. More than 93% of total fish catch constituted Liza spp. and Rutilus kutum. The relationship between the abundance and distribution of the studied species, and the restocking activities performed by Iranian fisheries organization are discussed. The high abundance and distribution of small size fish in tidal zone could be related to the distribution of crustacean, which provide a good source of food for these fish species.

Toxicological Effects of Heavy Metal Cadmium on Two Aquatic Species: Rutilus rutilus and Hypophthalmichthys molitrix

Introduction: Cadmium (Cd) is toxic to fish at low doses and never beneficial to an organism. As Caspian roach ( Rutilus rutilus ) and silver carp ( Hypophthalmichthys molitrix ) are two key fish species, the aim of this study was to gather data on different sensitivities of few inland fish to Cd to use them in the ecotoxicity experiment studies. Subject and Methods: All samples were exposed to the different doses of cadmium chloride (0, 0.2, 1, 2, 6, 10, and 15 ppm). Mortality was recorded after 24, 48, 72, and 96 h and the median lethal concentration (LC 50 ) amount and its confidence limits (95%) were measured by Finney’s method of probit analysis. Results: Toxicity experimenting statistical endpoints indicated that lowest observed effect concentration in roach was higher than silver carp (2 and 1 ppm, respectively), which means that no observed effect concentration was also higher for roach than silver carp (6 and 2 ppm, respectively), and LC 50 was also different between species (5.26 and 6.58 ppm for roach and silver carp, respectively). Conclusion: Our results showed that Cd is toxic for these fish, especially roach; therefore, we suggest using this fish species for toxicity experiment of heavy metals as a suitable indicator of toxicological studies.

The effects of garlic-supplemented diets on skin mucosal immune responses, stress resistance and growth performance of the Caspian roach (Rutilus rutilus) fry.

This study was conducted to evaluate the effects of garlic supplementation on some skin mucus immune parameters, mucus antimicrobial activity and growth performance of the Caspian roach (Rutilus rutilus caspicus) fry. Fish (1±0.07g) were divided into four groups fed diets containing 0 (control), 5, 10 and 15gkg(-1) garlic for 8 weeks. The results showed that there was a significant increase in weight gain and specific growth rate in those fish fed garlic diets compared with the control (P<0.05). Condition factor was not significantly affected by garlic dosage. At the end of trial, the epidermal mucus protein level, alkaline phosphatase and antimicrobial activity against 2g-negative bacteria (Escherichia coli and Serratia marcescens) and gram-positive bacteria (Streptococcus faecium and Micrococcus luteus) were measured. Skin mucus alkaline phosphatase, protein levels and antimicrobial activity were increased following garlic administration, and the bacterial growth inhibition zones were significantly elevated in garlic-fed fish (P<0.05). In salinity stress experiment, no differences were observed for survival rate among the experimental diets. No mortality was recorded during the feeding trial. These results indicated that dietary garlic beneficially affects the skin mucus immune parameters and growth performance of the Caspian roach fry.

The life-history traits of widespread freshwater fish species: the case of Roach Rutilus rutilus (Linnaeus, 1758) (Pisces: Teleostei) in water bodies at the southern limits of distribution

The Roach Rutilus rutilus (Linnaeus, 1758) (Pisces: Teleostei) is a widespread Eurasian cyprinid freshwater fish. Although numerous studies have investigated the species’ life history traits across its main native range of distribution, little is known from water bodies located beyond the southern limits of the latter, especially where the species has been introduced. Between July 2010 and April 2011, a total of 949 R. rutilus specimens was collected from Porsuk Reservoir (NW Turkey), and life-history traits of age, growth, fecundity and egg size were studied and compared to six other R. rutilus populations (including Caspian Roach R. r. caspicus) from the southern limits of distribution. The Porsuk population was characterised by relatively shorter lifespan, slower growth and lower fecundity. These findings support previous studies emphasising the importance of local environmental factors in shaping the life-history traits of widespread fish species. These must be taken into consideration especially in view of the potential value of R. rutilus as an economic resource for in-land fisheries.

The effect of the water soluble fraction of crude oil on survival, physiology and behaviour of Caspian roach, Rutilus caspicus (Yakovlev, 1870)

The water soluble fraction (WSF) of crude oil is a complex and toxic mixture of hydrocarbons that aquatic organisms directly encounter in oil spills. WSF plays an important role in the toxicity of crude oil to aquatic organisms. In the present study, the effects of WSF on juvenile Caspian roach, Rutilus caspicus, at lethal and sub-lethal level was investigated. The lethality of WSF on R. caspicus was studied by conducting 96h LC50 tests with semi-static exposure methods with 6 and 24h solution renewals. The 96h LC50 of WSF was estimated at 62.5% and 35.9% WSF concentrations for 24h and 6h renewal methods, respectively. To investigate the sub-lethal effect of WSF on R. caspicus, fish were exposed to 62.5, 31.3, and 6.3% concentrations of WSF for 24h and changes in their respiration rate and swimming activity was monitored during the exposure. At the end of the exposure period, four hematologic parameters [O2 and CO2 pressures (pO2 and pCO2), hematocrit, and hemoglobin content] of the fish were measured. The result of the behavioural experiment revealed that all three studied concentrations of WSF elevated the respiration rate and reduced the swimming activity of R. caspicus. No significant changes were detected in the hematocrit and hemoglobin content of the fish blood, but the blood pO2 of the fish exposed to 62.5% WSF decreased while the blood pCO2 increased. The results of this study suggest that the egression of the volatile components in hydrocarbon mixtures during conventional semi-static toxicity tests may lead to underestimating the toxicity of the hydrocarbons. The results of the sub-lethal experiments propose that failure of the respiratory system that leads to asphyxia may be a major mechanism that results in lethal effect of WSF in high concentrations.

The effect of sublethal concentrations of the water-soluble fraction of crude oil on the chemosensory function of Caspian roach, Rutilus caspicus (YAKOVLEV, 1870).

The water-soluble fraction of crude oil is a complex and toxic mixture of hydrocarbons. Because aquatic organisms directly encounter it, the water-soluble fraction plays an important role in the toxicity of crude oil in aquatic environments. To determine whether fish are attracted to or avoid the water-soluble fraction, Caspian roaches (Rutilus caspicus) were exposed to different concentrations of the water-soluble fraction in a choice maze apparatus. The results showed that Caspian roaches can detect and avoid 2 mg/L of the water-soluble fraction. To study the effect of the water-soluble fraction on the olfactory function of fish, Caspian roaches were exposed to 3.2 mg/L and 16 mg/L of the water-soluble fraction for 96 h; afterward, exposed fish encountered food extract in a choice maze apparatus. The present study showed that the water-soluble fraction significantly impairs the olfactory function of roaches. To investigate the effect of olfactory system dysfunction on the feeding behavior of fish, Caspian roaches were exposed to 3.2 mg/L and 16 mg/L of the water-soluble fraction. After 4 d, 8 d, and 12 d of exposure, the feeding behavior toward the food extract was tested. The results showed that both 3.2 mg/L and 16 mg/L of the water-soluble fraction suppress the feeding behavior of Caspian roaches. The present study demonstrates that sublethal concentrations of crude oil's water-soluble fraction impair the olfactory function of fish and consequently suppress the feeding behavior.

English

Pesticides are used in agriculture to control pest and protect human health and animals. Excessive use of pesticides caused risk for human health and threated non-target organisms, polluted water, soil and air. Hinosan is a component of organophosphate pesticide which is used as a fungicide in agricultural fields. In the study, lethal concentration (LC 50 ) of Hinosan was calculated for Rutilus frisii kutum and Rutilus rutilus caspicus with a mean weight of 3 ± 1 g [mean ± SD]. The experiment were carried out in static condition and based on instructions of O.E.C.D in four days under controlled water physicochemical factors with pH = 7 to 8.5, dissolved oxygen = 200 mg L -1 (CaCO 3 ) and temperature = 20 ± 1°C. Fishes were acclimatized in 70x40x30 cm aquarium for 10 days. Five treated aquariums with concentration ranges 1, 2, 4, 8, 16 ppm of hinosan (Technical 95 Edifenphos) with one control group (no toxic concentration), were performed. Data were analysed using the probit analysis. LC 1 , LC 10 , LC 30 , LC 50 , LC 70 , LC 90 and LC 99 were calculated in 24, 48, 72 and 96 h. Our results indicate that LC 50 96 h hinosan for R. frisii Kutum and R. rutilus caspicus were obtained 3.61 and 2.88 ppm, respectively. These findings suggest that hinosan is medium toxicity for these two species. Clinical symptoms including irregular protrusion of the eyes and irregular swimming were observed. Key words : Organophosphore, hinosan, Rutilus frisii kutum, Rutilus rutilus caspicus, LC50, pollution.

Effects of Sangrovit-supplemented diet on growth performance, blood biochemical parameters, survival and stress resistance to salinity in the Caspian roach (Rutilus rutilus) fry

This study was carried out to show the effects of Sangrovit on growth, blood biochemical parameters, survival and resistance to salinity stress in the Caspian roach. Fish (1.24 ± 0.13 g) were divided into four groups fed diets containing Sangrovit at different levels: 0 (control), 0.05, 0.1 and 0.15% for 45 days. The results showed that there was a significant increase in final weight, weight gain and specific growth rate in those fish fed 0.15% Sangrovit diets compared with the control (P < 0.05). Food conversion rate of fish fed 0.05% Sangrovit diet was significantly (P < 0.05) lower than the control group. Condition factor was not significantly affected by Sangrovit dosage (P > 0.05). The blood glucose and cholesterol levels were significantly lower in treated groups compared with the control (P < 0.05). The highest total protein was obtained in the 0.15% group and it was significantly higher than in all other groups (P < 0.05). Challenges were carried out after 45 days of feeding, to determine the effect of Sangrovit on resistance to salinity stress. Blood samples were obtained four times after challenge (day 1, 3, 5 and 7) for evaluate haematocrit levels. On the first day, haematocrit increased significantly in all groups compared to day 5 and 7 (P < 0.05). The haematocrit levels decreased gradually in all groups from the third day. Before and after stress, no differences were observed for survival rate among the experimental diets (P > 0.05). These results indicate that the inclusion of 0.15% Sangrovit in the diet of the Caspian roach enhances feed efficiency, fish growth and blood biochemical parameters.