To report the clinical features and best corrected visual acuity (BCVA) outcomes in patients with acute and delayed-onset endophthalmitis after open globe injuries. A retrospective, comparative, consecutive case series of patients with endophthalmitis after open globe injury between January 2016 and October 2020 at the Bascom Palmer Eye Institute. Acute-onset endophthalmitis accounted for 16/20 (80%) and all cases were diagnosed at the initial examination. Delayed-onset endophthalmitis cases, occurring more than two weeks after injury, accounted for 4/20 (20%) and were due to zone 1 wound leaks and infections. Factors associated with endophthalmitis included: presence of a retained intraocular foreign body (IOFB )11/20 (55%) and delay of presentation > 24 hours 15/20 (75%) (p<0.001, 0.002 respectively). The mean presenting BCVA was logMAR 1.64 (20/800) and the mean BCVA at last follow-up was logMAR 1.22 (20/300). In patients with open globe injury related endophthalmitis, VA outcomes are generally poor. In spite of intravitreal antibiotics at primary closure, delayed-onset endophthalmitis cases may develop in the setting of compromised zone 1 wound integrity.