Case Of Adenomatoid Odontogenic Tumor Research Articles

Clinical and demographic characteristics of adenomatoid odontogenic tumors: analysis of 116 new cases from a single center

IntroductionThe adenomatoid odontogenic tumor is a relatively uncommon odontogenic neoplasm representing about 4.7% of all odontogenic tumors. ObjectiveThe aim of this study was to determine the demographic and clinical profile of the adenomatoid odontogenic tumors in a Sri Lankan population. MethodsData gathered from the cases received for a period of 38 years from the Department of Oral Pathology, Faculty of Dental Sciences, University of Peradeniya. Request forms, biopsy reports and electronic data base of the department were used to obtain relevant information. Demographic data including age, gender and location of the tumor were included in the analysis. ResultsOut of 116 cases of adenomatoid odontogenic tumor, the mean age was 21.02 ± 11.24. It occurs more fre quently in the second decade of life, more prevalent in females, most often associated with the maxilla, predominantly affecting anterior jaw bones and presenting mostly in the right side of the jaw bone. The results from the present study showed the statistically significant relationship with site of occurrence (maxilla/mandible) and age (p < 0.005). Further, depending on whether it occurs in anterior/mid/posterior site also showed a significant relationship with age (p ≤ 0.001). However, side of occurrence, left or right or site of occurrence, showed no statistically significance with age (p > 0.05). ConclusionAdenomatoid odontogenic tumor occurs more frequently in the second decade of life with a significant female predominance and the commonest site is anterior maxilla. This study revealed few differences on demographic and clinical presentations of adenomatoid odontogenic tumor from some regions of the world.

FAK, paxillin, and PI3K in ameloblastoma and adenomatoid odontogenic tumor.

Integrins function to bind cells to extracellular matrix in tissues, which triggers downstream signaling cascades that are important in cell survival, proliferation, cytokine activation, and cytoskeleton reorganization. These processes also play significant roles in neoplasms. This work aimed to investigate the pattern of expression of FAK, paxillin, and PI3K in ameloblastoma and adenomatoid odontogenic tumor (AOT). Immunohistochemistry was used to study FAK, paxillin, and PI3K in 45 ameloblastomas (32 conventional, 12 unicystic, and 1 peripheral types), 7 AOTs, and two developing human teeth. Weak expression of FAK was seen in all AOT cases, while ameloblastoma had varying expression patterns, mostly strong to weak. The pattern of expression of paxillin and PI3K was relatively similar in both tumor types. In the dental germ, FAK and paxillin stained all the enamel organ components, while PI3K stained strongly the inner enamel epithelium. Stromal expression of FAK was not found to be useful in differentiating between tumors or tumor classes. The expression of the proteins in the enamel organ suggests that their signaling may be important in odontogenesis. While some ameloblastomas strongly expressed FAK, all cases of AOT had weak signals suggesting low presence and phosphorylating activity of FAK in the latter. A subset of FAK-positive ameloblastoma (as well as their malignant or metastasizing counterparts) which may have relatively aggressive behavior may be candidates for drug targeting of FAK as an additional management option.

An international collaborative study of 105 new cases of adenomatoid odontogenic tumors

The aim of this study was to report the clinicopathologic features of 105 new cases of adenomatoid odontogenic tumor (AOT) from Brazil, Guatemala, Mexico, and South Africa. Clinical and radiographic data were collected from records of 5 oral pathology laboratories, and all cases were microscopically reviewed. This series of cases included 36 (34.2%) from South Africa; 33 (31.4%) from Brazil; 23 (21.9%) from Mexico; and 13 (12.3%) from Guatemala. Seventy-two patients (68.5%) were females and 33 patients (31.4%) were males, with an average age of 19.2 years. The tumors predominantly affected the anterior maxilla (48.5%), followed by the anterior mandible (29.5%); posterior mandible (15.2%); and posterior maxilla (6.6%). Sixty-three tumors (63.6%) were follicular, 34 (34.3%) were extrafollicular, and 2 (2%) were peripheral. In most cases, the tumors caused considerable cortical expansion (average size 3.4 cm). Microscopically, most cases showed rosette-like and duct-like structures. This is one of the largest case series of AOTs reported in the literature and the first multicenter study involving populations from Latin America and South Africa. In a fifth of the present cases, the posterior regions of the gnathic bones were affected and the tumors demonstrated larger sizes compared with cases occurring in other populations.

Adenomatoid Odontogenic Tumor in the Posterior Mandible of a Young Child : A Case Report

Adenomatoid odontogenic tumor (AOT) is a rare benign odontogenic jaw lesion. It usually occurs in the anterior maxilla and is mostly related to impacted canines in teenagers.&lt;br/&gt;A 3-year-old girl was referred from a local dental clinic due to delayed eruption of the right primary mandibular 2nd molar. There was no history of pain or swelling. Radiography revealed a large radiolucency lesion with radiopacities around the unerupted right primary mandibular 2nd molar. Surgical enucleation with extraction of the right primary mandibular 2nd molar and surgical biopsy were performed. Based on the clinical and radiological findings, this lesion was defined as an ameloblastic fibro-odontoma which often develops in the mandible of adolescents. However, this lesion was diagnosed as AOT from the results of the histological examination.&lt;br/&gt;This report aimed to present a rare case of AOT in the posterior mandibular area in a very young patient.

Adenomatoid odontogenic tumor associated with impacted mandibular canine - A case report

Adenomatoid odontogenic tumor (AOT) is a relatively rare, benign, hamartomatous, and cystic odontogenic neoplasm that was first described more than a century ago. It accounts for 2-7% of all odontogenic tumors. The lesion still continues to intrigue experts with its varied histomorphology and controversies regarding its development. The present article describes a case of cystic AOT with an unusual histomorphology associated with an impacted 43 in a 15-year-old male.

Adenomatoid Odontogenic Tumor of Anterior Maxilla; A Case Report

Here, we report a case of adenomatoid odontogenic tumor in anterior maxilla in a young girl aged 14 years. Adenomatoid odontogenic tumor is a rare, benign, and distinct slow-growing odontogenic neoplasm which results in painless expansion of jaws. It usually occurs in the anterior maxilla followed by posterior maxilla and rarely in the mandible with an age range of 3 to 82 years and a relative frequency of 2.2% to 7.1% and showing a female predilection (1.9:1).

ADENOMATOID ODONTOGENIC TUMOR, THE REPORT OF THREE CASES

Adenomatoid odontogenic tumor (AOT) is a rare benign odontogenic tumor. AOT represents 3-7% of all odontogenic tumors. First described by Steensland in 1905 and later by Philipsen and Bin in 1969, AOT is an encapsulated tumor composed by odontogenic epithelium with duct-like structures. Radiographically, it commonly appears as a pericoronal unilocular radiolucency associated with an impacted tooth, more often the maxillary canine. Frequently, the lesion shows focal calcifications. Most cases are discovered in the second decade of life. Some authors have considered AOT as a hamartoma rather than a neoplastic process. A peripheral variant has been described. Here, we report three cases of AOT to illustrated the benign course of this tumor. Cases The three cases were found in females at the age of 15, 18 and 33 years. One case was discovered the mandible as periapical lesion and two cases associated with impacted maxillary canines. All cases show well-defined mixed radiolucent and radiopaque appearance. Root resorption was not observed in the mandibular case. Slow growing was reported in all cases. An excisional biopsy was done in the three cases. Microscopically, all cases revealed an encapsulated tumor compose of sheets of solid basaloid epithelium with duct-like spaces. Cystic and solid patterns with dystrophic calcification were also observed. No recurrence has been reported. Conclusion Although, the mandibular case was located in an uncommon location and showed an atypical appearance, all cases demonstrated an indolent behavior. These cases confirm the benign nature of this tumor.

Peripheral adenomatoid odontogenic tumor in a cloak of an epulis: report of a rare case

BackgroundAdenomatoid odontogenic tumor constitutes an uncommon benign odontogenic tumor which is well-known as “two-thirds tumor” (two-thirds of adenomatoid tumors occur in the maxilla, two-thirds occur in young females, two-thirds of the cases are associated with un-erupted teeth and two-thirds of the affected teeth are canines). Larger part of these present as intra - osseous tumors while peripheral counterparts are extremely rare. Peripheral adenomatoid odontogenic tumor is a unique entity which generally presents as a slow growing gingival swelling with minimal bone involvement. This often leads to its erroneous diagnosis as a simple gingival lesion and the real diagnosis is only revealed after its microscopic evaluation. It exhibits a marked predilection for maxillary gingiva of incisor region and most commonly affects the younger females.Case presentationWe report a case of 25 years old female patient of African ethnicity who presented with a 2 × 2 cm mass attached to the left mandibular gingiva in cuspid- bicuspid region which is an unusual site for AOT. It was accompanied by slight bone loss. With the differential diagnosis of gingival epulis and peripheral ossifying fibroma; surgical excision was performed. The light microscopic examination of the specimen aided the final diagnosis of Adenomatoid odontogenic tumor with the histopathological features identical to its intra osseous counterpart.ConclusionIn this case, the tumor is present on the mandibular gingiva in a 25 years old patient which is an atypical location and age for this tumor; also, the only individual case reported in an African patient. Only, a meager number of peripheral adenomatoid odontogenic tumor cases have been logged so far with majority of them occurring in maxillary gingiva. Furthermore, an ambiguity still prevails regarding its true origin and possible biological course. Hence, reporting of similar cases should be encouraged to facilitate the better understanding of its varied epidemiological details and clinical presentation.

Calretinin expression in odontogenic cysts and odontogenic tumors and the possible role of calretinin in pathogenesis of ameloblastoma.

Introduction:Histopathological diagnosis of odontogenic cysts and tumors is a task mostly accomplished with hematoxylin and eosin staining. However, the use of additional diagnostic modalities such as immunohistochemistry may be necessary in histologically similar lesions. The reports of studies which have used calretinin as an immunohistochemical marker for ameloblastoma have been conflicting.Aim:The aim of the study was to evaluate the use of calretinin as a specific diagnostic marker for ameloblastoma and observe its expression in odontogenic cysts and other odontogenic tumors.Materials and Methods:Formalin-fixed, paraffin-embedded sections were taken from the archives which included 15 cases each of dentigerous cyst, radicular cyst, odontogenic keratocyst and ameloblastoma five cases of adenomatoid odontogenic tumor and three cases of ameloblastic carcinoma. Immunohistochemistry was done with calretinin antibody.Results:All ameloblastomas were positive for calretinin, whereas no other tumor or cyst showed positivity. Differences in proportion of calretinin expression were statistically significant with P = 0.000.Conclusion:Calretinin can be considered as a specific marker for ameloblastomas.

A Case of Adenomatoid Odontogenic Tumor in the Maxillary Anterior Region of a Child

A Case of Adenomatoid Odontogenic Tumor in the Maxillary Anterior Region of a Child

Adenomatoid odontogenic tumor – A diagnostic imaging using cone bean computed tomography

Adenomatoid odontogenic tumor (AOT) is a rare noninvasive benign tumor of the jaw that originates in the second decade of the life from the odontogenic epithelium without mesenchyme. Radiological examination is an essential component for the diagnosis of benign tumors, the radiographic modality used to acquire the image was speculated to have an effect on the perception of radiopacities, particularly for the cases with minimal intralesional calcifications. Therefore, the capability of radiographic modality in detecting the intralesional calcification was critical for diagnosis of AOT. Cone beam computed tomography (CBCT) provides precise imaging of bony structures with minimal calcification without superimposition and distortion. In this case report, we are reporting a case of AOT of maxilla with characteristic CBCT appearance of AOT of maxilla.

Detection of MAPK/ERK pathway proteins and KRAS mutations in adenomatoid odontogenic tumors.

This study aimed to assess the frequency of KRAS mutation and its association with the presence of the MAPK/ERK signaling pathway proteins in adenomatoid odontogenic tumors. Paraffin-embedded tissue samples from nine cases of adenomatoid odontogenic tumor were used. Genomic DNA was extracted from each sample; in one case, genetic mutations in 50 cancer-associated genes were examined by next-generation sequencing. Hotspot mutations in the RAS family were analyzed by Luminex assay using the remaining eight cases. Subsequently, immunohistochemistry for KRAS, CRAF, BRAF, EGFR, ERK, MEK, and BRAFV600E was performed. A KRAS G12D missense mutation was detected in the DNA sequence of the tumor cells, but it was not detected in the stromal tissue. KRAS G12V and KRAS G12R mutations were detected in two and four cases, respectively. For immunohistochemistry, all the cases were EGFR, KRAS, BRAF, CRAF positive, one case was ERK negative,and one case was MEK and ERK negative, all the other remaining cases were MEK and ERK positive. KRAS mutation at codon 12 and the presence of MAPK/ERK pathway proteins were detected suggesting their association with tumorigenesis of adenomatoid odontogenic tumors.

Oct-4 and CD44 in epithelial stem cells like of benign odontogenic lesions

Benign epithelial odontogenic lesions are great clinical importance entities that develop in the jaws from the tissues that form teeth. It has been shown that benign and malignant tumors are present in a large number of tumor stem cells, which has great implications in the development of these lesions. Oct-4 and CD44 have been demonstrated as important markers for tumoral stem cells. The aim of this study was investigate the presence of stem cell markers Oct-4 and CD44 in benign epithelial odontogenic lesions. Twenty odontogenic keratocysts (OKC), 20 ameloblastomas (AMB) of the solid/multicystic type and 20 adenomatoid odontogenic tumors (AOT) were retrospectively analyzed for immunohistochemical detection of Oct-4 and CD44 in their epithelial component. All cases were positive for the two markers, with the majority exhibiting a high expression. Analysis of the expression of Oct-4 revealed no statistically significant differences (p = 0.406) between the lesions studied. Regarding CD44, there was a significant difference between the cases of AMB and AOT in relation with OKC, with the latter presenting a greater labelling (p = 0.034). No statistically significant correlation between Oct-4 and CD44 was observed in the lesions. In our findings, the presence of stem cell-like phenotype at various sites of the epithelial component of the odontogenic lesions was identified, suggesting its possible participation in histogenesis and differentiation without, however, exerting influence on the aggressiveness of the lesions.

A Case of Adenomatoid Odontogenic Tumor of the Maxillary Sinus

A Case of Adenomatoid Odontogenic Tumor of the Maxillary Sinus

A Unique case of Adenomatoid Odontogenic Tumour of Lateral Incisor: Pathogenetic Evaluation through Immunohistochemistry

A Unique case of Adenomatoid Odontogenic Tumour of Lateral Incisor: Pathogenetic Evaluation through Immunohistochemistry

A Unique case of Adenomatoid Odontogenic Tumour of Lateral Incisor: Pathogenetic Evaluation through Immunohistochemistry

A Unique case of Adenomatoid Odontogenic Tumour of Lateral Incisor: Pathogenetic Evaluation through Immunohistochemistry

Adenomatoid odontogenic tumour: An enigma.

Adenomatoid odontogenic tumour (AOT) is an uncommon, hamartomatous, benign epitheial lesion of odontogenic origin. The current World Health Organisation (WHO) classification of odontogenic tumors defines AOT as being composed of the odontogenic epithelium in a variety of histoarchitectural patterns, embedded in mature connective tissue stroma, and characterized by slow, but progressive growth. The aim of this paper is to present three rare cases of intraosseous AOTs with varied clinical and radiographic features imposing the fact that AOT should be included in differential diagnosis of routine odontogenic cysts and tumours.

Adenomatoid odontogenic tumor: Clinical and radiological diagnostic challenges

The adenomatoid odontogenic tumor (AOT) is a relatively uncommon odontogenic tumor with a relative frequency of 3–7%. The tumor is more common in females in their second decade of life. It exhibits a predilection for the maxillary anterior region. In this paper, we present a case of AOT in the mandible of a 27-year-old female. Histopathological examination of the tissue obtained from the initial incisional biopsy showed mostly an epithelial-lined cyst, which included a small nodule with the characteristic histological features of an AOT. Subsequent enucleation was done that confirmed the AOT diagnosis. The uncommon location of the lesion and the predominantly cystic configuration posed a clinical diagnostic challenge.

Adenomatoid odontogenic tumor: Case series of 14 with wide range of clinical presentation.

BackgroundTo present 14 cases of adenomatoid odontogenic tumor (AOT), highlighting their clinical, radiographic, and histologic characteristics.Material and MethodsFourteen cases of AOT were retrieved from the archives of the Oral Pathology Biopsy Service (OPBS). Clinical, radiologic, and histologic findings are described.ResultsFourteen AOT cases were reviewed, of which 12 were intraosseous and two were peripheral (gingiva). The cases came from eight females and six males with an age range of 11–30. Of the 12 intraosseous cases, nine were follicular (associated with impacted teeth), while three were extra-follicular (present between teeth). Six of the 12 cases were in the maxilla, and the other six were in the mandible. The two peripheral cases presented as nodules on the buccal gingiva of the anterior maxillary teeth. Radiographically, all 12 follicular and extra-follicular cases were unilocular radiolucencies; of those, only one had specks of radiopacity. Histologically, all specimens were similar in morphology, demonstrating a varied degree of duct-like structures, epithelial spheres, spindle-shaped epithelial cells, calcifications, and a thick capsule. The two peripheral cases had no capsule.ConclusionsAOT usually affects patients under 20 years of age, with a female to male ratio close to 2:1. Presentation in the anterior maxilla is almost twice as common as in the anterior mandible. Radiographically, AOT presents as a unilocular radiolucency more commonly associated with impacted teeth, simulating a dentigerous cyst. We present 14 new cases of AOT (nine follicular, three extra-follicular, and two peripheral) with discussions of their clinical, radiographic, and histological features. Key words:Adenomatoid, odontogenic, tumor.

COMPARATIVE STUDY OF CALRETININ EXPRESSION IN ODONTOGENIC CYSTS AND TUMORS

Calretinin is a calcium-binding protein with molecular weight of 29 kDa (KiloDalton). It is expressed in a variety of normal and tumorigenic tissues. Its expression in odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. This study was undertaken to evaluate the expression of Calretinin in a group of odontogenic cysts and tumors. The expression of Calretinin was evaluated immunohistochemically in 42 samples including 8 cases of periapical cyst, 7 cases of dentigerous cyst, 7 cases of keratocystic odontogenic tumor, 8 cases of solid (multicystic) ameloblastoma, 6 cases of unicystic ameloblastoma and 6 cases of adenomatoid odontogenic tumor. Positive immunohistochemical reaction was found in all cases of solid ameloblastoma (100%), in 4 cases of unicystic ameloblastoma (67%) and 4 cases of keratocystic odontogenic tumors (57%) whereas none of the other odontogenic cysts and adenomatoid odontogenic tumors included in this study showed reactivity. Intensity was higher in the ameloblastomas compared with the keratocystic odontogenic tumors. On the basis of these results, it is suggested that calretinin might be used as a specific immunohistochemical marker for the ameloblastomas and could play an important role in the differentiation of aggressiveness of different odontogenic tumors.