Carnation Varieties Research Articles

IN VITRO PRODUCTION OF VIRUS-FREE CARNATION (DIANTHUS CARIOPHYLLUS L.) PLANTING MATERIAL

The methods of culture of apical meristems and direct and indirect morphogenesis in vitro were used for production of virus-free planting material of carnation. A scheme for obtaining aseptic material has been developed, which consists of stepwise treatment of explants: Thimerosal - 2 min, 70% ethyl alcohol - 0.5 min and 0.08% AgNO 3 - 1 min, which reduces the level of contamination by fungal infection. Expounded the results of studies of callusogenesis and direct and indirect morphogenesis in the culture of in vitro explants of Dutch carnation, their dependence on the content of growth regulators in the nutrient medium. It was established that there were almost no significant differences in the course of callusogenesis processes within carnation varieties . At the same time, the frequency of callusogenesis was 100%. Under the conditions of indirect morphogenesis realization, it is necessary to take into account the age of callus tissues. The growth and intensive shoot formation of carnations was noted on the Murashige-Skoog nutrient medium supplemented with BAP at a concentration of 0.5 mg/l. The best medium for rooting was the MS medium with half the concentration of macro- and microsalts with the addition of 0.5 mg/l of NAA, which is recommended by us for rooting regenerating carnation plants of various varieties. Peat : perlite in a 1:1 ratio was used as a substrate for the adaptation of regenerating plants . Survival of carnation plants to conditions in vivo for the variety "Raffino Linde" was 90%, while for the variety "Tiya" - 83%, respectively.

Differences in ethylene sensitivity, expression of ethylene biosynthetic genes and vase life among carnation varieties

Differences in ethylene sensitivity, expression of ethylene biosynthetic genes and vase life among carnation varieties

Varietal Evaluation of Carnation (Dianthus caryophyllus) in Naturally Ventilated Polyhouse under Prayagraj Agro-climatic Condition

Carnation, scientifically known as Dianthus caryophyllus, is a popular and attractive flowering plant that belongs to the Caryophyllaceae family. Native to the Mediterranean region, carnations are now cultivated all over the world for their beauty and fragrance. Therefore, present investigation was carried out at the Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, Uttar Pradesh during the Winter-2022-23 with a view to determine the performance of different varieties of carnation for its growth and flowering under ventilated polyhouse. Under this experiment, overall, 6 varieties were used comprising of variety Cerventes, Master, Magno, Baltico, Realism and Kiro. It was concluded that variety Baltico (white) reported significantly better performance compared to other varieties, in terms of Growth parameters like plant height was seen in cervantes (25.19 cm), number of leaves were maximum in master (20.94), number of internodes was highest in realism (18.49), number of branches were seen to be maximum in baltico (4.47), Kiro was found to be less suited variety for Prayagraj agro climatic condition due to metabolic activity. Flowering parameters like days to first flower bud break (65.33 days), days taken for first flower bud opening (66.00 days) was seen in Master, Flower Stalk length was seen highest in Realism (40.80 cm), number of flowers per plant was seen in cervantes (5.17), number of days taken for flower senescence on plant was seen highest in kiro (15.40 days), the vase life in carnation was seen to be the highest in master (0.40 days).

Response of Carnation Varieties (Dianthus caryophyllus) to Mycorrhizal Fungi under Naturally Ventilated Polyhouse Conditions in Prayagraj, India

The present investigation entitled, Response of carnation varieties (Dianthus caryophyllus) to mycorrhizal fungi under naturally ventilated polyhouse conditions in Prayagraj was conducted at horticulture research field, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P, during the winter season (2022). The experiment was laid out in Factorial Randomized block design (FRBD) with 3 varieties and 5 VAM treatments in 15 treatments and each replicated thrice. From the present investigation, Variety-3 (Kiro-yellow) treated with 10g VAM per plant performed better for plant height (55.44cm), primary branches (7.22), bud diameter (21.72mm), and yield per plant (5.74). In terms of flower diameter (6.16cm) and vase life (13.33 days) Variety-1 (Cervantes) treated with 10g VAM performed better. In terms of first bud initiation Variety-2 (Baltico-White) treated with 5g VAM per plant showed better performance (59.11 days).

Variation in longevity of cut and in planta flowers of potted carnation varieties affected by their relationship with ethylene and water

Carnation (<italic>Dianthus caryophyllus</italic> L.) is a typical ethylene-sensitive cut flower, but a few differences in ethylene sensitivity have been reported for different potted carnation species. In this study, we investigated the relationship between vase life, ethylene sensitivity, ethylene biosynthesis gene expression, and flower volatile substance content of 17 different potted carnation varieties. It was found that under the same post-harvest environmental conditions, the vase life of different varieties ranged from 6.2−14.2 d. Among the 17 varieties, 'Cherry' and 'Grace Bay' were highly sensitive to ethylene, and qRT-PCR analysis showed that their ethylene biosynthesis genes <italic>DcACS1</italic> and <italic>DcACO1</italic> expression increased the most, while 'Pink and Purple', 'Bondi Beach', and 'Grane Beach' showed the opposite pattern. In addition, the lower ethylene release was important in leading to longer vase life of potted carnations, and that ethylene release from shorter vase life varieties was 2.5−4.5 times greater than that of longer vase life varieties. Varieties that are more sensitive to ethylene are more likely to have a shorter vase life due to early disruption of water relations, which is mainly the result of reduced stem hydraulic conductivity and transpiration water loss. Analysis of volatile substances showed that ethylene had no significant effect on the release of volatile substances from potted carnations. Therefore, a better understanding of petal senescence in potted carnations will help us to improve measures to extend flower longevity according to the ethylene sensitivity of different varieties.

Response of some carnation varieties to Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria

Carnation is one of the most crucial cut flowers in Turkey. Root-knot nematodes (RKNs) cause significant damage on carnation production and quality. Therefore, determination of response of carnation varieties to RKNs is required for management practices. In this study, four carnation cultivars, Turbo, Betsy, Nirvana and Glaciar, were inoculated with second stage juveniles (J2s) of Meloidogyne incognita, M. javanica and M. arenaria under controlled conditions. Meloidogyne incognita, M. javanica and M. arenaria did not reproduce on Turbo variety. Therefore, Turbo variety was found to be resistant to these nematodes. Nirvana variety was moderately resistant to M. incognita and M. javanica, but highly resistant to M. arenaria. Betsy and Glaciar varieties were susceptible to M. incognita, M. javanica and M. arenaria. Results showed that the response of carnations was different based on root knot nematodes. Determining resistant carnation varieties could be an advantage for the control of RKNs. Resistant carnations could be used to control the RKNs and develop new carnation varieties.

Performance of Carnation (Dianthus caryophyllus L) Varieties under Protected Condition

The present investigation was undertaken to elevate different carnation varieties for the performance of Growth, yield and post-harvest quality under protected condition. The experiment was conducted in Horticultural Research Station, Tamil Nadu Agricultural University, The Nilgiris, India . The experiment was laid out in a Randomized Block Design (RBD) with Eleven varieties of Carnation viz., Big one, Turbo, Solex, Hunza, Easy Golem, Express Golem, Red King, Golem, Gioele, Big mama and Happy Golem. observation were recorded for plant height (cm), days to first flower, flowering duration, length of flower stem (cm), diameter of flower (cm), Number of flowers per plant and Calyx splitting (High : 60% and above, Medium: 30 – 50%, Low : less than 30%), Strength of flower stem, vase life solutions viz., AOA (25ppm), AOA (50ppm), CoCl2 (10ppm) ,CoCl2 (20ppm) and plain water for 30 minutes. The cultivar Happy Golem recorded maximum plant height of 100.50cm and the minimum plant height of 77.0 cm was noticed in Golem. Maximum number of shoots per plant was recorded in the cultivar Gioele (7.98 ) followed by Hunza (7.50) and minimum number of shoots per plant was observed in the entry Golem (5.30).Maximum number of leaves (195.20) and Leaf length (13.55 cm) was recorded in the entry Gioele. The Days to first flower ranged between 130.01 to 139.20 days from planting. The cultivar Turbo (130.01) recorded early to initiate the flower bud followed by Golem (130.10) .The cultivar Red king recorded maximum flowering duration of 13.50 days followed by big mama (13.20 days)) and minimum flowering duration was observed in Happy Golem (10.47 days).The Cultivar Gioele recorded the highest stalk length of 93.00 cm and the lowest stalk length of 68.50cm was observed by Golem. With respect to yield parameters maximum number of flowers per plant was recorded in Gioele (7.50 nos) followed by Red king (7.17 nos) and Hunza (6.97 nos) and Minimum number of flowers per plant was recorded in Golem (5.11 nos). Maximum number of A and B grade flower stems are produced in the Variety Gioele. Among the cultivar for prolonging the vase life of Carnation, treatment comprising of AOA @50ppm recorded maximum vaselife of 19 days by cultivar, minimum flower diameter (5.3cm) and maximum total water absorbed (45.0 ml) and found to be the best to increase the shelf life of carnation.

Differential Response of Carnation Varieties (Dianthus caryophyllus L.) to Different Levels of Boron under Polytunnels

Carnation (Dianthus caryophyllus L.) is one of the most commercially important cut flowers of the world. It can be used for cut flowers, bedding, edging, boarders, pots and rock garden. Four Spanish carnation varieties were tested with three levels of boron under ultra-violet stabilized polytunnels from August 2017 to April 2018 at Godawari, Nepal. The objective of the research was to evaluate vegetative, floral, and vase life parameters in relations to growth, yield and quality of carnation. The research was conducted in a randomized complete block design with 3 replications. The variety Baltico had the maximum leaf length (19.3 cm), numbers of lateral branches (10.5), internodal length (5.1 cm), flower diameter (8.6 cm), and vase life (24.0 days). The numbers of nodes (5) and flower weight (25.4 g) were highest in the variety Vinco with the lowest cracked area on the stem (0.2 cm2/ crack). The numbers of stem cracks per plant significantly (p ≤0.01) reduced with an increasing level of boron though non-significant relation was observed on 60 ppm and 120 ppm. Variety Baltico was found as a better variety compared to the Vinco, Cervantes and Master varieties according to the vegetative, floral, yield and vase life parameters. Among the levels of boron tested, 60 ppm was found as the optimum dose. Thus, variety Baltico and 60 ppm boron is suggested to be used at the climatic condition of Lalitpur.

Effect of pinching on growth of carnation varieties grown under protected condition

An experiment was conducted to study the effect of pinching methods on carnation under protected condition. The results of present investigation revealed that variety Rendez-vous (V 4 ) recorded significantly the highest plant height (28.99cm, 39.81 cm and 35.33 cm) at all the stages. Similarly, maximum number of leaves per plant (214.07), number of internodes per stem (17.20) was observed in variety Rendez-vous (V 4 ). While, variety Gaudina (V 1 ) recorded significantly the highest internodal length (12.26cm).Variety Liberty (V 2 ) recorded significantly the maximum number of auxiliary branches per plant (5.35). Significantly the highest stem length (103.37 cm), were observed in variety Farida (V 3 ).

To study the effect of pinching on yield and quality of carnation varieties grown under protected condition

An experiment was conducted to study the effect of pinching methods on carnation under protected condition. The results of present investigation revealed that the variety Liberty (V 2 ) recorded significantly the highest number of flowers per plant (6.23), number of flowers per square meter (205.70) and flower yield per hectare (2077778 Numbers). As per quality point of view the variety Farida (V 3 ) recorded significantly the highest vase life (12.33 days) and longevity of flowers (16.98 days). The treatment P 3 (Double pinch) resulted in significantly the highest number of flowers per plant (6.75), number of flowers per square meter (222.75) and flower yield per hectare (2250000 Numbers). The treatment P 1 (Single pinch) recorded significantly the highest vase life (11.94 days) and longevity of flowers (15.61 days).

English

Carnation (Dianthus caryophyllus L.) is a cut flower with greater stipulation in the world cut flower market. In India, carnations are cultivated under polyhouses in Nilgiris and Kodaikanal districts in the state of Tamil Nadu. Carnations cultivation is impeded by various diseases, among them stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary was found to be predominant in all varieties. Survey among commercially cultivated varieties of carnation during 2013, revealed the occurrence of stem rot incited by Sclerotinia sclerotiorum, for the first time in India. The pathogen was identified as Sclerotinia sclerotiorum on the basis of phenotypic and genotypic characteristics. Carpogenic germination was induced artificially, and life cycle of the fungi was studied. Microscopic studies of the apothecium revealed the presence of spermatia, croziers, paraphyses as well as monomorphic ascospores specific to S. sclerotiorum. Key words: Apothecium, carnation, croziers, India, Sclerotinia, spermatia.

Identification of heat-responsive genes in carnation (Dianthus caryophyllus L.) by RNA-seq.

Carnation (Dianthus caryophyllus L.) is an important flower crop, having substantial commercial value as a cut-flower due to the long vase-life and wide array of flower colors and forms. Standard carnation varieties perform well under cool climates but are very susceptible to high temperatures which adversely affect the yield and the quality of the cut-flowers. Despite several studies of carnation contributing to the number of expressed sequence tags (ESTs), transcriptomic information of this species remains very limited, particularly regarding abiotic stress-related genes. Here, transcriptome analysis was performed to generate expression profiles of heat stress (HS)-responsive genes in carnation. We sequenced a cDNA library constructed with mixed RNA from carnation leaves subjected to 42°C HS (0, 0.5, 1, and 2 h) and 46°C HS (0.5, 1, and 2 h), and obtained 45,604,882 high quality paired-end reads. After de novo assembly and quantitative assessment 99,255 contigs were generated with an average length of 1053 bp. We then obtained functional annotations by aligning contigs with public protein databases including NR, SwissProt, KEGG, and COG. Using the above carnation transcriptome as the reference, we compared the effects of high temperature treatments (42°C: duration 0.5, 2, or 12 h) delivered to aseptic carnation seedlings, relative to untreated controls, using the FPKM metric. Overall, 11,471 genes were identified which showed a significant response to one or more of the three HS treatment times. In addition, based on GO and metabolic pathway enrichment analyses, a series of candidate genes involved in thermo-tolerance responses were selected and characterized. This study represents the first expression profiling analysis of D. caryophyllus under heat stress treatments. Numerous genes were found to be induced in response to HS, the study of which may advance our understanding of heat response of carnation.

Standardization of foliar nutrients (NPK) spray in carnation (Dianthus caryophyllus L.) varieties under protected condition

The present experiment entitled, standardization of foliar nutrients (NPK) spray in carnation (Dianthus caryophyllus L.) varieties under protected condition was carried out under polyhouse, at Department of Horticulture, College of Agriculture, Junagadh Agricultural University, Junagadh during 2013. The experiment consisted of three varieties with five levels of fertilizers and it was laid out in Factorial Completely Randomized Design Design with three replications. The results of present investigation on vegetative growth parameters revealed that variety Don Pedro Rapido with nutrient level 6000 N: 4000 P2O5: 2000 K2O ppm recorded highest plant height, fresh and dry weight of plant, internodes per stem and internodal length, harvesting span, fresh and dry weight of single cut flower, number of petals per flower, vase life of cut flower, in-situ longevity of flower, length of flower stalk, number of flowers per plant, m2 and hectare, minimum days taken to flower bud initiation, open of first flower. Whereas, only stalk thickness were observed in variety Madame Colette with 6000 N: 4000 P2O5: 2000 K2O ppm treatment.

Sequence Analysis of the Genome of Carnation (Dianthus caryophyllus L.)

The whole-genome sequence of carnation (Dianthus caryophyllus L.) cv. ‘Francesco’ was determined using a combination of different new-generation multiplex sequencing platforms. The total length of the non-redundant sequences was 568 887 315 bp, consisting of 45 088 scaffolds, which covered 91% of the 622 Mb carnation genome estimated by k-mer analysis. The N50 values of contigs and scaffolds were 16 644 bp and 60 737 bp, respectively, and the longest scaffold was 1 287 144 bp. The average GC content of the contig sequences was 36%. A total of 1050, 13, 92 and 143 genes for tRNAs, rRNAs, snoRNA and miRNA, respectively, were identified in the assembled genomic sequences. For protein-encoding genes, 43 266 complete and partial gene structures excluding those in transposable elements were deduced. Gene coverage was ∼98%, as deduced from the coverage of the core eukaryotic genes. Intensive characterization of the assigned carnation genes and comparison with those of other plant species revealed characteristic features of the carnation genome. The results of this study will serve as a valuable resource for fundamental and applied research of carnation, especially for breeding new carnation varieties. Further information on the genomic sequences is available at http://carnation.kazusa.or.jp.

Evaluation of carnation (Dianthus caryophyllus L.) varieties under naturally ventilated greenhouse in mid hills of Kumaon Himalaya

Among eight Carnation varieties viz. Diana, Aurturo, White Dona, Pink Dona, Soto, Red King, Tuareg and Dona., evaluated under naturally ventilated greenhouse and subjected to uniform treatment and cultural package of practices, the variety Red King was found best with respect to number of branches (8.0), number of flowers/plant (35.6), fresh weight of flower (8.38 g), dry weight of flower (2.66 g), flower diameter (7.83 cm) and vase life an important post harvest quality parameter was observed to be the superior in variety Red King (29.3 days) followed by cv Tuareg (24 days) and Pink Dona (21.3 days). On the basis of present experiment it was concluded that cultivar Red King were found to be promising with respect to yield as well as flower quality parameters and found suitable for commercial cultivation under naturally ventilated green house in mid hills of Kumaon Himalayas. Key words: Carnation, greenhouse, varieties, vase life.

Expression of Flavonoid 3′,5′-Hydroxylase and Acetolactate Synthase Genes in Transgenic Carnation: Assessing the Safety of a Nonfood Plant

For 16 years, genetically modified flowers of carnation ( Dianthus caryophyllus ) have been sold to the floristry industry. The transgenic carnation carries a herbicide tolerance gene (a mutant gene encoding acetolactate synthase (ALS)) and has been modified to produce delphinidin-based anthocyanins in flowers, which conventionally bred carnation cannot produce. The modified flower color has been achieved by introduction of a gene encoding flavonoid 3',5'-hydroxylase (F3'5'H). Transgenic carnation flowers are produced in South America and are primarily distributed to North America, Europe, and Japan. Although a nonfood crop, the release of the genetically modified carnation varieties required an environmental risk impact assessment and an assessment of the potential for any increased risk of harm to human or animal health compared to conventionally bred carnation. The results of the health safety assessment and the experimental studies that accompanied them are described in this review. The conclusion from the assessments has been that the release of genetically modified carnation varieties which express F3'5'H and ALS genes and which accumulate delphinidin-based anthocyanins do not pose an increased risk of harm to human or animal health.

Structure of the acyl-glucose-dependent anthocyanin 5-O-glucosyltransferase gene in carnations and its disruption by transposable elements in some varieties

The pink, red and crimson petal colors of carnations (Dianthus caryophyllus) are produced by anthocyanins. The anthocyanins, pelargonidin and cyanidin can be modified by two glucoses at the 3 and 5 positions, and by a single malic acid. Petal color variation can result from failure of such modification, for example, the lack of a glucose at the 5 position is responsible for the color variants of some commercial varieties. With respect to this variation, modification by 5-O-glucosyltransferase plays the most important role in glucosylation at the 5 position. Recently, we identified a novel acyl-glucose-dependent anthocyanin 5-O-glucosyltransferase (AA5GT), that uses acyl-glucoses, but not UDP-glucose, as the glucose donor. Although we showed that loss of AA5GT expression was responsible for loss of glucosylation at the 5 position of anthocyanin in some varieties, the cause of this repression of AA5GT expression could not be determined. Here, we have succeeded in isolating the AA5GT gene and found that it consists of 12 exons and 11 introns. In carnation varieties lacking a glucose at the 5 position, we identified the insertion of two different retrotransposons, Ty1dic1 and Retdic1, into AA5GT. Ty1dic1, which belongs to the class I long terminal repeat (LTR)-retrotransposons of Ty1/copia families, was inserted into exon 10. Retdic1, which includes a long interspersed nuclear element (LINE)-like sequence, was inserted into intron 5. Thus, insertion of either Ty1dic1 or Retdic1 can disrupt AA5GT and result in the lack of glucosylation at the 5 position in anthocyanins.

Validation of a Carnation-Specific Gene, ANS, Used as an Endogenous Reference Gene in Qualitative and Real-Time Quantitative PCR for Carnations

The validation of the anthocyanin synthase (ANS) gene as a carnation endogenous reference gene applicable both in classical and real-time PCR methods is a prerequisite for the development of PCR assays for genetically modified (GM) carnation detection. This is important due to the fact that GM carnation lines, developed by Florigene Pty Ltd, have been approved for commercialization. In this study, both methods were tested on 14 different carnation cultivars, and identical amplification products were obtained with all of them. No amplification products were observed with samples from 14 other plant species, which demonstrated that the system was specific to carnation. The results of Southern blot analysis confirmed that the ANS gene had a low copy number in the 10 tested carnation varieties. In qualitative and real-time PCR assays, the LOD values of 0.05 and 0.005 ng carnation DNA, respectively, were validated. Moreover, the real-time PCR system was validated with high PCR efficiency and linearity. Thus, the ANS gene had species specificity, low heterogeneity, and low copy number among the tested cultivars. These results provide evidence that the gene can be used as an endogenous reference gene of carnation, as well as in qualitative and quantitative PCR systems.

Genetic modification in floriculture

Micro-propagation, embryo rescue, mutagenesis via chemical or irradiation means and in vitro inter-specific hybridisation methods have been used by breeders in the floriculture industry for many years. In the past 20 years these enabling technologies have been supplemented by genetic modification methods. Though many genes of potential utility to the floricultural industry have been identified, and much has been learnt of the genetic factors and molecular mechanisms underlying phenotypes of great importance to the industry, there are only flower colour modified varieties of carnation and rose in the marketplace. To a large extent this is due to unique financial barriers to market entry for genetically modified varieties of flower crops, including use of technology fees and costs of regulatory approval.

IDENTIFICATION OF CARNATION VARIETIES USING MICROSATELLITE MARKERS

As in many ornamentals, also in carnation the number of varieties in common knowledge is large and identification throughout the chain from breeder to consumer using plant material from different stages and organs may be needed. Results in this study on the use of microsatellite markers from Dianthus caryophyllus L. for the characterization of carnation varieties as well as the construction and evaluation of a molecular database show that these markers show potential for identification purposes