Abstract Background: The rationale for failed peripheral venous catheter (PVC) care was found to be the nature of complication, lack of time, type and amount of fluid infused, drug administered, nurse: patient ratio, and lack of resources. Revision and reinforcement of policy, protocol, and guidelines on PVC care guidelines are mandatory for quality patient care. Aim: The present study aimed to assess the nurse’s perception of the peripheral venous catheter (PVC) care guidelines among nurses. Methodology: A cross-sectional study was conducted among 44 registered nurses in pediatric wards of a tertiary care hospital in Eastern India. The data collection tool includes nurses’ sociodemographic data, a Likert scale on the perception of nurses toward PVC care guidelines implementation, and a semi-structured interview guide to conduct focus group discussion on nurse’s opinions toward PVC care guidelines. Results: Majority (84.1%) of the nurses had a neutral perception, and less than one-fourth (9.1%) had a positive perception of PVC care guidelines. The focus group discussion found three themes including “Revision to PVC care guidelines,” “Problems in providing PVC care,” and “Merits of PVC care guidelines.” Conclusion: Frequent updating through the in-service session on PVC care guidelines helps nurses to understand the PVC care procedure better for quality patient care. However, other factors to be considered include the availability of resources, staff workload, and work environment for nurse’s better adherence.