Background and Aims: Ascitic fluid infections (AFI) in cirrhosis, excluding the secondary causes, can be classified into Spontaneous bacterial peritonitis (SBP), Culture negative neutrophilic ascites (CNNA) & Non-neutrocytic monobacterial bacter ascites (BA). AFI accounts for 10%–30% of reported bacterial infections among cirrhotic patients. This study aims to analyse frequency and variants of SBP, causative organisms and their antibiotic sensitivity pattern in our setting. Methods: This is retrospective analysis of 202 ascitic samples of clinically suspected cases of AFI in cirrhosis. Samples were collected from April 2017 to April 2018. Ascitic fluid was collected by bedside inoculation in blood culture bottle aseptically. Culture and antibiotic sensitivity tests were carried out using automated incubator (BACT/ALERT 3D) and automated sensitivity technique (VITEK 2). Results: Of 202 clinically suspected cases, 100 (49.51%) had ascitic fluid infection; of which 89 (89%) were males & 11 (11%) females; mean age was 54.7 years & mean MELD score 18.3. 54 (54%) were CNNA & 46 (46%) were culture positive (41% SBP & 5% BA). From 46 culture positive cases, organisms isolated were E. coli 25 (54.34%), Klebsiella 5 (10.86%), Staphylococcus 5 (10.86%), Pseudomonas 4 (8.69%), Enterococcus 3 (6.52), Streptococcus 2 (4.35%) & Acinetobacter 2 (4.35%). Gram negative bacteria (78.26%) were most commonly seen, E. coli (54.34%) was the predominant organism. 77.8% of gram negative & 80% of gram positive bacteria were sensitive to 3rd generation cephalosporins (ceftriaxone, cefotaxim, cefoperazone). Of 25 E. coli, 21 were sensitive to 3rd generation cephalosporin, 2 were resistant to betalactamase inhibitors, and there was no resistance to quinolones and carbapenams. 80% of klebsiella, 75% of pseudomonas and 80% of staphylococcus were sensitive to 3rd generation cephalosporins, one methicillin resistant staphylococcus aureus (MRSA) was seen, which was sensitive to carbapenam. Acinetobacter were multidrug-resistant, sensitive only to imipenem and colistin. Conclusions: CNNA were more than culture positive cases. Gram-negative organisms dominated ascitic fluid cultures, highest was E. coli. 3rd generation cephalosporins may be continued as empirical 1st line treatment. The authors have none to declare.