Carcinogenic Properties Research Articles

Influence of Some Heterocyclic, Cyclic, and Nitrogen-Containing Compounds on Oxidative Deamination of Polyamines in a Cell-Free Test System.

We studied the effects of some nitrogen-containing, heterocyclic, and cyclic compounds on the rate of oxidative deamination of polyamines and putrescine in tissues with a high proliferation rate. For this purpose, the specific activities of the main enzymes of polyamine oxidative degradation - spermine oxidase (SMO), polyamine oxidase (PAO), and diamine oxidase (DAO) were determined using a cell-free test system from regenerating rat liver. The compounds methyl 2-(5-formylfuran-2-yl)benzoate and 2,7-bis-[2-(diethylamino)ethoxy]-9H-fluoren-9-one (and in the form of dihydrochloride) showed mainly activating effect on oxidative degradation of putrescine, spermidine, and spermine, which indirectly indicates their antiproliferative effect. Nitrogen-free compounds inhibited this process, thus exhibiting potentially carcinogenic properties. Correlations were calculated for activity of DAO, PAO, and SMO with 5 topological indices: Wiener (W), Rouvray (R), Balaban (J) in the Trinaistich modification, detour (Ip), and electropy (Ie). The highest dependence was noted for DAO and the Balaban index (R=-0.55), for PAO and the detour index (R=0.78), and for SMO and the electropy index (R=0.53). The remaining dependencies showed insignificant correlation strength.

Preliminary quality/ price analysis for some Betula products

Species from Betula genus have been proven to have bioactive features, including anti-inflammatory, anti- carcinogenic and anti-rheumatic properties. They are either used for therapeutic or pharmacological purposes. There are a variety of types of products, from the well-known sap to tinctures or extracts, having different flavors, for a wider range of preferences. Thus, the purpose of this study was to compare the quality of liquid products produced from birch sap, from various companies and harvested naturally, in relation to the price of each product. This study required a thorough monitoring of prices over a period of several months, as well as qualitative and quantitative analyses. For the investigation of these indices, 6 types of products were used. The price rise research was conducted between November 2023 and January 2024, with 11 samples drawn from each type. All these tests reveal a link between the price of a product and its quality; thus, the more expensive products have a greater amount of sugar, which shows that the products have been preserved well.

IMPACT OF THE SPACE INDUSTRY ON CANCER INCIDENCE IN THE POPULATION OF KAZAKHSTAN: A LITERATURE REVIEW

Relevance: Resolving the impact of the space industry on cancer incidence comes down to assessing and preventing the environmental risks caused by the Earth’s surface contamination with space debris and rocket fuel combustion waste. Components of liquid rocket fuel, like unsymmetrical dimethylhydrazine (UDMH) and others, have proven general toxic, carcinogenic, teratogenic, and mutagenic properties. The research available in the world uses calculations of the individual inhalation and oral carcinogenic risk of the effect of UDMH and its derivative N-nitrosodimethylamine on public health. Cancer incidence is an indicator of the specific influence of carcinogenic and co-carcinogenic environmental factors on public health. The Ulytau region is particularly interesting since separating parts of launch vehicles usually fall there, and sub-route areas are located there. From 2020 to 2022, the Karaganda region, including districts and cities that later became part of the Ulytau region, ranked 5th in cancer incidence in Kazakhstan. The study aimed to analyze published data on cancer incidence among the population of the Ulytau region living near the areas where the separated parts of launch vehicles fell and determine the direction of further research. Methods: A literature search in the PubMed database and archives of Kazakhstani scientific editions revealed 35 literature sources that met the search criteria. The analysis included 29 scientific publications examining the health status of a population exposed to rocket and space activities, including reference materials. Data on morbidity and mortality from neoplasms in the population of several districts of the Karaganda region was analyzed. Results: Research data analysis showed conflicting conclusions regarding the influence of rocket fuel components on the health of the population living in the zones of influence of the rocket and space activities. Some researchers found increased morbidity and mortality in the population, but others did not reveal statistically significant differences with control groups. An analysis of data on the mortality of the population living near the regular and emergency areas of fall of separating parts of launch vehicles in the Karaganda region showed an increased mortality rate for some nosologies. Conclusion: No studies have compared the cancer incidence in the Ulytau region’s population living in areas adjacent to the fall areas of separating parts of launch vehicles with the control areas.

Experimental exploration and DFT analysis of the kinetics and mechanism of malachite green photodegradation catalyzed by polyaniline-copper oxide nanocomposite.

A nanocomposite photocatalyst consisting of polyaniline (PANI) and copper oxide (CuO) was successfully synthesized through an in-situ polymerization approach using aniline as the precursor. The synthesized nanocomposite was characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV-Vis), determination of the point of zero charge (pHPZC), and scanning electron microscopy (SEM). The photocatalytic efficiency of the PANI-CuO nanocomposite was evaluated in the context of photodegrading Malachite Green (MG) dye under visible light. Malachite Green, a synthetic dye commonly used in the textile and aquaculture industries, is a significant contaminant due to its toxic, mutagenic, and carcinogenic properties, making its removal from water resources crucial for environmental and human health. Distilled water artificially contaminated with MG dye was used as the medium for testing. The parameters influencing the photodegradation efficiency were comprehensively investigated. These parameters included catalyst dosage, reaction time, initial dye concentration, and pH. The results of this study indicate that the degradation efficiency of MG dye displayed an upward trend with time, catalyst dosage, and pH while exhibiting a converse relationship with the initial dye concentration. A degradation rate of 97% was achieved with an initial concentration of 20mgL-1, employing a catalyst dose of 1.6gL-1 at pH 6 for a reaction time of 180min. Furthermore, the reusability of the catalyst was assessed, revealing consistent performance over five consecutive cycles. Density functional theory (DFT) was employed to optimize the structures of PANI, PANI-CuO, and their respective complexes formed through dye interaction, employing Gaussian software. These calculations employed the B3LYP/6-311G + + (d,p) basis set in an aqueous environment with water serving as the solvent. The kinetics of Malachite Green degradation were analyzed using both first and second-order kinetic models.

Uncovering an effecient binary system as a chemosensor for visual and fluorescence detection of chromium (VI) in water samples

There is an urgent requirement for the development of sensitive and quick sensors to monitor chromium (VI) due to its substantial carcinogenic and mutagenic properties. A coexisting system of coumarin 334 and diphenylcarbazide (C334/DPC) was used in this study as a fluorescent chemosensor to detect Cr(VI) ions. Upon the addition of Cr(VI), a purple chelate complex (Cr(III)-diphenylcarbazone) was produced, which resulted from the quantitative reaction between Cr(VI) ions and diphenylcarbazide (DPC), whereas no interaction between Cr(VI) and coumarin 334 took place. More interestingly, the absorption spectra of purple (Cr(III)-diphenylcarbazone) complex (λmax = 540 nm) were overlapped with emission and excitation spectra of coumarin 334 (λex/em = 453/492), resulting in the efficient quenching of coumarin 334 (C334) via the inner filter effect. Furthermore, the semi-quantitative estimation of Cr(VI) ion concentration may be achieved by visually watching the progressive color transformation of the probe from yellow to red after the addition different concentration of Cr(VI). The calibration plot for determination of Cr(VI) by this method is ranging from 0.048 to 268 μM. DFT calculations were conducted to enrich our understanding about the mechanism of action. This approach demonstrates an excellent selectivity and sensitivity for Cr(VI) including a detection limit of 48 nM. The new sensor was successfully applied to water samples (tap, mineral, and waste waters). The accuracy was confirmed by the atomic absorption spectroscopy.

Long-term graft survival in a kidney transplant recipient with glioblastoma: Case report

Long-term immunosuppression after transplantation can increase the risk of cancer development in recipient patients. This case report describes the treatment approach for glioblastoma in a kidney transplant recipient after transplantation. The patient, a 61-year-old woman, received a living donor kidney transplant 24 years ago due to congenital nephrotic syndrome. The patient was on various immunosuppressive medications, including cyclosporine, prednisolone, and mycophenolate mofetil.After 16 years of follow-up, the patient presented with symptoms of brain tumor, leading to further tests. Subsequent examination revealed the presence of a tumor that had spread to frontal region within the brain.A surgical procedure was subsequently conducted to extract the tumor cells and alleviate the resulting pressure within the brain. Based on pathology results, it was determined that the patient had glioblastoma.Methylation of the O6-methylguanine-DNA methyltransferase (MGMT) promoter was detected, indicating the potential response to chemotherapy. Chemotherapy was initiated, along with radiation therapy.After the diagnosis and surgery, the patient's medications for the kidney transplant were modified. Rapamycin replaced the previous medications, and the dose of mycophenolate mofetil and prednisolone was decreased. After 7 years, the patient's kidney is functioning well, with a creatinine level of 1.5, and brain imaging showed no abnormalities. After kidney transplantation, there is an increased risk of various cancers.Overall, this case report demonstrates a successful treatment approach for glioblastoma after kidney transplantation, emphasizing the need for close monitoring and individualized management in transplant recipients at risk for cancer development.Considering the current stability of the patient's condition after a change in medication regimen, patients who have been using the drug Cyclosporine for a long time should be included in future evaluations due to its carcinogenic properties.

Polycyclic Aromatic Hydrocarbons' Impact on Crops and Occurrence, Sources, and Detection Methods in Food: A Review.

Polycyclic aromatic hydrocarbons (PAHs) represent a category of persistent organic pollutants that pose a global concern in the realm of food safety due to their recognized carcinogenic properties in humans. Food can be contaminated with PAHs that are present in water, air, or soil, or during food processing and cooking. The wide and varied sources of PAHs contribute to their persistent contamination of food, leading to their accumulation within these products. As a result, monitoring of the levels of PAHs in food is necessary to guarantee the safety of food products as well as the public health. This review paper attempts to give its readers an overview of the impact of PAHs on crops, their occurrence and sources, and the methodologies employed for the sample preparation and detection of PAHs in food. In addition, possible directions for future research are proposed. The objective is to provide references for the monitoring, prevention, and in-depth exploration of PAHs in food.

The effect of plant extracts on the microbiological parameters of grain

Currently, ensuring high quality and food safety is an important condition for a rational diet, maintaining health, mental and physical performance and supporting the body's defense systems. The current ecological situation is one of the factors determining the nutritional status of the population. Extracts of leaves and stems of rosehip, sea buckthorn and barberry were used as plant raw materials. The use of these plant raw materials is explained by the fact that their extracts of leaves and stems contain polyphenols with antioxidant, antimicrobial and carcinogenic properties. The effective amount of extracts of plant raw materials has been established. The obtained extracts were injected in an amount of 0.05% by weight of their solids. The optimal duration of hydration is 6 and 12 hours, but depending on the effectiveness of the time ‒ 6 hours. The grain is moistened at 23 ° C (room temperature). The effect of plant extracts on the microbiological sowing of grain during soaking and preparation of grain for production and prevention of microbial destruction of bakery products during storage is investigated. Extracts of rosehip leaves and sea buckthorn stems showed a pronounced antimicrobial effect against fungi of the genus Pénicillium. This eliminates the diseases of bread and improves its rheological properties.

A water-soluble red-emitting fluorescence probe for detecting hazardous hydrazine in environmental waters and biosystems

Hydrazine (N2H4), a crucial chemical raw material, enhances people's lives and fosters human progress. Hydrazine usage or leakage has caused environmental contamination, affecting water, soil, and living beings. Hydrazine simultaneously presents a possible risk to human health due to its carcinogenic properties. Thus, quick and precise detection of hydrazine is crucial in environmental studies and biological contexts. We prepared a red-emitting fluorescence turn-on probe (XT-HZ) to detect hydrazine specifically. The probe has a low detecting limit for hydrazine (63 nM) with excitation wavelength at 570 nm and emission wavelength at 625 nm. Besides, the probe XT-HZ had excellent water solubility, high selectivity, and good sensitivity for detecting hydrazine. Finally, probe XT-HZ was applied in the imaging of N2H4 in living cells, zebrafish and environmental water samples.

Fungal allergens and mold allergy diagnosis

Immunology is a branch of medicine that studies the immune system and its pathology. There are known scientific information, that in medical immunology specific tests performed in the laboratory for diagnosis. In addition, good to mention that allergies include medical diagnostic methods such as blood tests. Also, there are used for detection the presence and the levels of IgE antibodies. Currently within the present description aim to discuss some aspects referring to fungal allergen and mold allergy diagnosis, in a shortage following steps of our purposed description.Molds produce mycotoxins, unfortunately with carcinogenic, teratogenic and neurotoxic properties, with a possible negative impact on individual healthy. Allergies related to fungi together with comorbidities, mainly concern immunocompromised patients. The currently describing knowledges in the field of allergy between fungal allergens and human subjects, and also established the diagnosis and treatment, offer us a complex vision. For the future, hope to find into the research studies, proper knowledges in this field, with applicability in routine practice.

Phenethyl isothiocyanate inhibits the carcinogenic properties of hepatocellular carcinoma Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways.

Hepatocellular carcinoma (HCC) is an aggressive malignancy with limited effective treatment options. Phenethyl isothiocyanate (PEITC) is a bioactive substance present primarily in the cruciferous vegetables. PEITC has exhibited anti-cancer properties in various cancers, including lung, bile duct, and prostate cancers. It has been demonstrated that PEITC can inhibit the proliferation, invasion, and metastasis of SK-Hep1 cells, while effectively inducing apoptosis and cell cycle arrest in HepG2 cells. However, knowledge of its anti-carcinogenic effects on Huh7.5.1 cells and its underlying mechanism remains elusive. In the present study, we aim to evaluate the anti-carcinogenic effects of PEITC on human HCC Huh7.5.1 cells. MTT assay and colony formation assay was performed to investigate the anti-proliferative effects of PEITC against Huh7.5.1 cells. The pro-apoptosis effects of PEITC were determined by Annexin V-FITC/PI double staining assay by flow cytometry (FCM), mitochondrial transmembrane potential (MMP) measurement, and Caspase-3 activity detection. A DAPI staining and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was conducted to estimate the DNA damage in Huh7.5.1 cells induced by PEITC. Cell cycle progression was determined by FCM. Transwell invasion assay and wound healing migration assay were performed to investigate the impact of PEITC on the migration and invasion of Huh7.5.1 cells. In addition, transcriptome sequencing and gene set enrichment analysis (GSEA) were used to explore the potential molecular mechanisms of the inhibitory effects of PEITC on HCC. Quantitative real-time PCR (qRT-PCR) analysis was performed to verify the transcriptome data. MTT assay showed that treatment of Huh7.5.1 cells with PEITC resulted in a dose-dependent decrease in viability, and colony formation assay further confirmed its anti-proliferative effect. Furthermore, we found that PEITC could induce mitochondrial-related apoptotic responses, including a decrease of mitochondrial transmembrane potential, activation of Caspase-3 activity, and generation of intracellular reactive oxygen species. It was also observed that PEITC caused DNA damage and cell cycle arrest in the S-phase in Huh7.5.1 cells. In addition, the inhibitory effect of PEITC on the migration and invasion ability of Huh7.5.1 cells was assessed. Transcriptome sequencing analysis further suggested that PEITC could activate the typical MAPK, PI3K-Akt, and p53 signaling pathways, revealing the potential mechanism of PEITC in inhibiting the carcinogenic properties of Huh7.5.1 cells. PEITC exhibits anti-carcinogenic activities against human HCC Huh7.5.1 cells by activating MAPK/PI3K-Akt/p53 signaling pathways. Our results suggest that PEITC may be useful for the anti-HCC treatment.

Novel composite from chitosan and a metal-organic framework for removal of tartrazine dye from aqueous solutions; adsorption isotherm, kinetic, and optimization using Box-Benkhen design

Cosmetics, textiles, foodstuffs, and medicines frequently contain the yellow dye tartrazine. It has carcinogenic properties and can trigger allergies. In this study, a unique NH2-MIL-101(Cr)/chitosan composite (MIL/chitosan composite) was created using a hydrothermal process. The effectiveness of this composite in removing Tartrazine (TZ) from aqueous solutions was investigated. It was characterized via FT-IR, XPS, XRD, and BET analysis. The surface area of the MIL/chitosan nanoadsorbent sample was 1256.64 m2/g, where after five times recycling, it was reduced to 1068.14 m2/g. The study analyzed the impact of dye concentration, pH, temperature, and MIL/chitosan composite dosage. Experimental measurements were taken for the equilibrium isotherms of dye adsorption. The kinetic models and adsorption isotherm were used to analyze the results. The adsorption process was found to match Langmuir and pseudo-second-order kinetic models. Chemisorption was the mechanism of the adsorption process. Based on thermodynamic parameters, it was determined that the adsorption process was endothermic. The MIL/chitosan composite was recycled up to five cycles. Using the MIL/chitosan composite towards the adsorption of the tartrazine from the real sample has been checked. The interaction process between the MIL/chitosan nanoadsorbent and Tartrazine adsorbate has been investigated. The TZ electrical characteristics, reactivity, and shape were ascertained through the application of density functional theory (DFT). The placement of electrophilic and nucleophilic attack sites is in good agreement with the molecular orbitals (HOMO and LUMO) and MEP results, according to DFT. The optimization of adsorption results was accomplished using Box-Behnken design (BBD).

Promotion Strategy for OHS Management of Chemical Hazard Factors in Hospitals: Literature Review

Introduction: OHS or Occupational Safety and Health in hospitals is one of the activities carried out to maintain and protect human resources so that they can protect themselves and patients. Apart from that, human resources in the hospital sector are not only aimed at doctors, but also at patient companions, patients, employees, staff and all components involved in the hospital. Objective: Analyze OHS promotion management strategies regarding chemical hazard factors in hospitals. Method: The method used in the research uses a qualitative type. The approach used is a descriptive literature review. Result: There are various types of chemical hazards found in hospitals, for example found in laundry services where the ingredients contain quite high risks and dangers. For example, clax allegro has been proven to have corrosive and carcinogenic properties. Harmfull has the property of causing eye damage and burns. Anios has flammable vapor properties, sensitizer, and so on. Conclusion: One of the factors that needs to be considered in the management strategy is related to the management of medical waste which is related to chemical factors. The remaining chemicals used in hospitals or what is called waste must first go through several processes, for example sorting, storing, transporting and managing according to the characteristics of the chemicals found in the hospital. The management carried out has the aim of minimizing the dangers posed by the chemicals used in the hospital

An Review: On Nutritive Activity and Ayurvedic Property of Piper Betel(Paan)

Piper betel L.comes in family Piperaceae and called Paan leaves in india . Piper betel or Betel vine deep heart shaped .This betel leaf widely shown in Sri Lanka, India, Thailand, Taiwan and other Southeast Asian countries. The oil of paan may be used as an industrial raw material for manufacturing medicines, perfumes, mouth fresheners, tonics, food additives etc. The betel leaves are shows nutritive property as well as carcinogenic property which is very usefull for manufacturing of blood cancer drug which is greatest advantage of betel leaf. Their leaves have been used as a traditional medicine to treat various health conditions. This paan is highly abundant and inexpenxsive, therefore it promotes the further research and industrialization development, including in the food and pharmacuitical industries. P. betle leaves displayed high efficiency on Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria such as Staphylococcus aureus, and Candida albicans. Piper betel is mostly use to chew with sliced areca nut, slaked lime, coriander, aniseed, clove, cardamom, sweetener, coconut scrapings etc, but less used remedy. This paan can cure many diseases and reduce the oral cancer which actually happens due to sliced areca nut, slaked lime not because of betel leaves.It contains high amount of nutrient like water,protein , energy , fats , fibres ,calcium etc. y. It is cultivated in hotter and damper part incountry following the traditional methods in India on about 55,000 hectare with an annual production worth about Rs 9000 million. It shows antimicrobial activity, antidiabetic ,Gastroprotective , platelet inhibition activity ,Antifertility like other onens properties ,also they shows the antioxidant , antiallergic , anti coagulant , anti inflammatory , radioprotective , anticancer potential like activities .

Analısıs Kualıtatıf Rhodamın B pada Perona Mata (Eyeshadow) yang Beredar dı Toko Kosmetık Kota Tarakan

Both adults and teenagers often use eyeshadow as a common cosmetic. The paint and textile industry often uses a red synthetic dye known as rhodamine B. Apart from having carcinogenic properties, continued use of rhodamine B can cause liver problems and gastrointestinal irritation which has the potential to hurt health. This research aims to answer the problem, of whether or not eyeshadow preparations sold in Tarakan City beauty shops contain Rhodamine B. The method in this research uses a wool thread technique and a rhodamine B rapid test kit to carry out qualitative analysis using eleven samples. The results showed that of the eleven eyeshadow samples examined at the Tarakan City cosmetics shop, three samples experienced a color change from red to dark red or purplish. Two of the eyeshadows are not registered, while one is registered with BPOM. Based on these findings, it can be concluded that three of the eleven eyeshadow samples tested positive for rhodamine B.

High capacity adsorption of antimony by hollow Co-MnO2 and its consequential utilization in SbO2--based aqueous batteries

Toxic metal pollution is one of the environmental problems that seriously affect water resources and ecosystems. Antimony, recognized for its teratogenic and carcinogenic properties, poses significant health risks due to its widespread presence in natural water sources. In this study, cobalt-doped manganese oxide bimetallic composites were designed as an efficient adsorbent for the antimony removal from water. The adsorbent exhibits robust performance over a range of pH values, achieves a significant adsorption capacity of 591.1 mg/g, and exhibits adsorption equilibrium within 25 min. The effectiveness of the adsorption is attributed to the interaction between metal-O bonds and antimony, as well as the hydrogen bonding. In line with the concept of sustainable development, waste adsorbents are used as negative electrodes for SbO2--based aqueous alkaline batteries. It exhibits a high reversible specific capacity of 122.8 mAh·g−1. This research not only sheds light on innovative approaches to antimony removal but also opens up avenues for the sustainable reuse of waste materials, in line with the principles of sustainable development.

Fungal Isolation, Detection, and Quantification of Aflatoxins in Nuts Sold in the Lebanese Market

This study examines the prevalence of aflatoxin contamination in 160 nut samples, both shelled and unshelled (including pistachios, peanuts, and walnuts), from the Lebanese market, focusing on their fungal contamination and specific toxigenic strains. Aflatoxin B1 (AFB1), known for its potent carcinogenic and immunosuppressive properties, was detected in various samples. Moisture content analysis showed that unshelled nuts often exceeded maximum moisture limits more frequently than shelled nuts, with levels ranging from 1.9 to 9.5%. The predominant fungal genus identified through cultivation on potato dextrose agar (PDA) plates was Aspergillus. In total, 55% of samples were contaminated with A. flavus and 45% with A. niger. All toxigenic strains isolated were identified as Aspergillus flavus. The aflatoxins, particularly AFB1, were quantified using the enzyme-linked immunosorbent assay (ELISA) and reversed-phase high-performance liquid chromatography (HPLC), revealing contamination in 43.8% of the samples, with concentrations ranging from 0.4 to 25 µg/kg. Some samples notably exceeded the established maximum tolerable limits (MTLs) for AFB1, set between 2 and 8 µg/kg. Shelled pistachios showed the highest contamination rate at 52% and were the most frequent to surpass the MTL of 8 µg/kg for pistachios, whereas walnuts displayed the lowest contamination levels, with only 15.4% exceeding the MTL for aflatoxins.

Exploring novel insights into the molecular mechanisms underlying Bisphenol A-induced toxicity: A persistent threat to human health

Bisphenol A (BPA) is a ubiquitous industrial chemical used in the production of polycarbonate plastics and epoxy resins, found in numerous consumer products. Despite its widespread use, its potential adverse health effects have raised significant concerns. This review explores the molecular mechanisms and evidence-based literature underlying BPA-induced toxicities and its implications for human health. BPA is an endocrine-disrupting chemical (EDC) which exhibits carcinogenic properties by influencing various receptors, such as ER, AhR, PPARs, LXRs, and RARs. It induces oxidative stress and contributes to cellular dysfunction, inflammation, and DNA damage, ultimately leading to various toxicities including but not limited to reproductive, cardiotoxicity, neurotoxicity, and endocrine toxicity. Moreover, BPA can modify DNA methylation patterns, histone modifications, and non-coding RNA expression, leading to epigenetic changes and contribute to carcinogenesis. Overall, understanding molecular mechanisms of BPA-induced toxicity is crucial for developing effective strategies and policies to mitigate its adverse effects on human health.

Development and application of a protocol for extractables profiling from central venous catheters in neonates

Peripherally inserted central catheters (PICC-lines) used in neonatology are made of thermoplastic polyurethane (TPU) or silicone. These materials usually contain substances that may leach into drug vehicles or blood. In this extractables study, we determined the optimal extraction conditions using TPU films containing defined amounts of butylhydroxytoluene (BHT) and then applied them on unused and explanted PICC-lines. Maceration and sonication tests were carried out with hexane, acetone and water as the extraction solvents. The analyses were performed using gas and liquid chromatography coupled with mass spectrometry detectors, as well as inductive coupled plasma optical emission spectroscopy to detect a wide range of extractables. We selected a limited list of substances to be sought from the usual adjuvants and monomers, related to their carcinogenic, mutagenic or reprotoxic properties and/or existence in endocrine disruptors lists. The TPU-film experiments showed that acetone was slightly better than hexane, and maceration better than sonication. When applied to PICC-lines, the extraction methods were almost similar but acetone was clearly better than hexane for TPU. From the 48 peaks initially observed in GC-MS, we ended up with 37 peaks to follow in TPU PICC-lines, among which were those of BHT and 4,4′-Methylenebis(cyclohexyl isocyanate) isomers. For silicone PICC-lines, out of 41 peaks initially observed in GC-MS, we followed 20 peaks, most of them being identified as cyclosiloxanes. Barium was the main inorganic element extracted for both PICC-lines. For TPU PICC-lines, the inter-batch variability was higher than for intra-batch, but in silicone devices both were similar. When compared to new PICC-lines, explanted TPU PICC-lines extracted peaks had a lower area under the curve (AUC), while the AUCs of the peaks were higher for the majority of silicone PICC-lines extract compounds. No identified substances were detected above their toxicological threshold, but isocyanates and cyclosiloxanes toxicity was mostly studied for other exposition routes than intravenous. The methods defined in this study were efficient in producing extractable profiles from both PICC-lines.

A highly selective dual-signal response ratiometric fluorescence sensing strategy for malachite green in fish based on carbon dots/copper nanoclusters nanocomposite

Malachite green (MG), a widely used antiparasitic agent, poses health risks to human due to its genotoxic and carcinogenic properties. Herein, a stable dual-emission fluoroprobe of carbon dots/copper nanoclusters is prepared for highly selective detection of MG based on the inner filter effect. This probe exhibits characteristic emission bands at 435 and 625 nm when excited at 376 nm. After adding MG, the both emission signals were significantly quenched, and the ratio of fluorescence intensity (F435/F625) was linearly related to the concentration of MG in the range of 0.05–40 μmol L−1 with a limit of detection of 18.2 nmol L−1. Meanwhile, the two signals exhibit linear relationships with the concentration of MG, respectively, and the corresponding detection results were consistent. The fluoroprobe was successfully used for the detection of MG in fish samples with the recoveries ranging from 96.0% to 103.8% and a relative standard deviation of <3.3%.