Carcharocles megalodon is considered a macropredatory shark that inhabited the seas around the world from middle Miocene to late Pliocene. In Argentina, it has only been formally recorded at two localities. Here, we report the first record for this taxon in the Buenos Aires Province. This occurrence is based on an isolated tooth recovered on the beach at the Punta Médanos locality, which lacks clear stratigraphic context. Based on the regional geology, the specimen probably came from Pliocene beds. Its size indicates that it probably belongs to a juvenile individual. Keywords: Carcharocles megalodon, macropredatory shark, fossil teeth, Mar de Ajó.