Carboxylate-modified Polystyrene Research Articles

Ecotoxicological effects of polystyrene nanoplastics on common carp: Insights into blood parameters, DNA damage, and gene expression.

Plastics are ubiquitous in modern society due to their cost-effectiveness, lightweight nature, and versatility. However, their extensive use and inadequate recycling have led to a significant environmental challenge, with plastic waste accumulating rapidly and causing ecological and health problems, especially in aquatic environments. Nanoplastics, particles ranging from 1 to 100 nm, have emerged as a particularly concerning subset due to their ability to easily penetrate biological barriers and accumulate in tissues. In this study, we investigated the toxicity of carboxylate-modified polystyrene nanoplastics (PS-NPs) on common carp (Cyprinus carpio), a species often used in ecotoxicology research due to its ability to accumulate pollutants. The PS-NPs were characterized, and their effects on DNA damage gene expression related to oxidative stress and immunity were examined. PS-NPs with a diameter of 20-30 nm were found to possess a spherical shape and negatively charged surfaces. Exposure to PS-NPs led to significant DNA damage in the blood and brain cells of common carp, with higher concentrations resulting in more severe damage. Additionally, PS-NP exposure influenced the expression of genes related to antioxidative defense and stress response in the liver. Specifically, genes encoding superoxide dismutase (SOD), catalase (CAT), and heat shock protein 70 (Hsp70) showed upregulation, while glutathione peroxidase (GPx) and glutathione S-transferase (GST) exhibited downregulation at higher PS-NP concentrations. Furthermore, the immune-related genes interleukin-1ß (IL-1ß), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) displayed dose-dependent downregulation in the liver tissue. These findings suggest that exposure to PS-NPs induces oxidative stress, disrupts immune responses, and causes DNA damage in common carp. The results highlight the need for further research on the environmental impacts of PS-NPs and underscore the importance of proper waste management and recycling practices to mitigate plastic pollution.

Uptake and effect of carboxyl-modified polystyrene microplastics on cotton plants

Microplastics (MPs) have emerged as a significant global environmental concern, particularly within agricultural soil systems. The extensive use of plastic film mulching in cotton cultivation has led to the alarming presence of MP pollution in cotton fields. However, the uptake and effects of MPs on the growth of cotton plants are poorly understood. In this study, we conducted a comprehensive analysis of hydroponically cultured cotton seedlings at the phenotypic, transcriptional, and metabolic levels after exposure to carboxyl-modified polystyrene microplastics (PS-COOH). Treatment with three concentrations of PS-COOH (100, 300, and 500 mg/L) resulted in notable growth inhibition of treated plants and exhibited a dose-dependent effect. And, PS-COOH can invade cotton roots and be absorbed through the intercellular spaces via apoplastic uptake, with accumulation commensurate with treatment duration. Transcriptomic analysis showed significant up-regulation of genes associated with antioxidant activity in response to 300 mg/L PS-COOH treatment, suggesting the induction of oxidative stress. In addition, the PS-COOH treatment activated the phenylpropanoid biosynthesis pathway, leading to lignin and flavonoid accumulation, and altered sucrose catabolism. These findings illustrate the absorption and effects of MPs on cotton seedlings and offer valuable insights into the potential toxicity of MPs to plants in soil mulched with plastic film.

Enhancing the Sensitivity of Lateral Flow Assay with Europium Nanoparticles for Accurate Human IgG Quantification.

Accurate quantification of immunoglobulin G (IgG) levels is vital for understanding immune status and diagnosing various medical conditions. Lateral flow assays (LFAs) offer rapid and convenient diagnostic tools, but their sensitivity has been a limitation. Our research introduces a refined method incorporating europium nanoparticles, enhancing both sensitivity and accuracy of LFAs in human IgG measurement. Utilizing a unique sandwich format, carboxylate-modified polystyrene Eu (III) chelate microparticles (CM-EUs) acted as the primary reporters. The concentrations of both detection and capture antibodies on the strip were optimized to bolster the LFA's quantitative performance. The subsequent calibration curve between the IgG concentration and the measured intensity ratio (VR) established the linearity and analytical sensitivity of our method with a high correlation coefficient (r = 0.99) and an impressively low limit of detection (LoD = 0.04 ng/mL). Our precision assessment, segmented into intra-assay and inter-assay evaluations, showcases the method's consistency and reproducibility. The LFA assay's stability was established by demonstrating its resistance to degradation and affirming its potential for extended storage without a dip in performance. The study's findings underscore the potential of this method to contribute to diagnostic medicine and improve patient care.

A novel reagent for the screening of haptoglobin-deficient blood donors.

In Japan, the prevalence of haptoglobin deficiency is approximately 1 in 4000. Haptoglobin-deficient individuals may produce anti-haptoglobin from allo-immunization, leading to serious transfusion reactions. Therefore, implementation of a consistent supply of haptoglobin-deficient fresh frozen plasma is crucial. We developed a novel reagent to facilitate large-scale identification of haptoglobin-deficient individuals as potential donors of plasma products. We established mouse monoclonal anti-haptoglobin-producing cell lines (three clones) using the hybridoma method by immunizing mice with the haptoglobin protein. Purified antibodies were conjugated with carboxylate-modified polystyrene latex beads and used for haptoglobin measurements by the latex agglutination method using an automatic analyser (LABOSPECT008). Samples with low protein concentrations were re-examined by enzyme-linked immunosorbent assay to confirm the results. Additionally, the haptoglobin gene was amplified by polymerase chain reaction to confirm the haptoglobin deletion allele (Hpdel ). From February to October 2022, 7476 blood donor samples were screened. Two haptoglobin-deficient and 21 low-haptoglobin-expressing individuals were identified. Two haptoglobin-deficient donors were found homozygous for Hpdel , and 19 (90%) of the 21 low-haptoglobin-expressing individuals were heterozygous for Hpdel , which includes the first reported case of heterozygous Hpdel /HpJohnson . We developed a new reagent for the detection of haptoglobin deficiency, which is automatable and inexpensive and appears useful for large-scale screening of blood donors.

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

Human respiratory mucus is a biological hydrogel that forms a protective barrier for the underlying epithelium. Modulation of the mucus layer has been employed as a strategy to enhance transmucosal drug carrier transport. However, a drawback of this strategy is a potential reduction of the mucus barrier properties, in particular in situations with an increased exposure to particles. In this study, we investigated the impact of mucus modulation on its protective role. In vitro mucus was produced by Calu-3 cells, cultivated at the air-liquid interface for 21 days and used for further testing as formed on top of the cells. Analysis of confocal 3D imaging data revealed that after 21 days Calu-3 cells secrete a mucus layer with a thickness of 24 ± 6 μm. Mucus appeared to restrict penetration of 500 nm carboxyl-modified polystyrene particles to the upper 5–10 μm of the layer. Furthermore, a mucus modulation protocol using aerosolized N-acetylcysteine (NAC) was developed. This treatment enhanced the penetration of particles through the mucus down to deeper layers by means of the mucolytic action of NAC. These findings were supported by cytotoxicity data, indicating that intact mucus protects the underlying epithelium from particle-induced effects on membrane integrity. The impact of NAC treatment on the protective properties of mucus was probed by using 50 and 100 nm amine-modified and 50 nm carboxyl-modified polystyrene nanoparticles, respectively. Cytotoxicity was only induced by the amine-modified particles in combination with NAC treatment, implying a reduced protective function of modulated mucus. Overall, our data emphasize the importance of integrating an assessment of the protective function of mucus into the development of therapy approaches involving mucus modulation.

Deposition behaviors of carboxyl-modified polystyrene nanoplastics with goethite in aquatic environment: Effects of solution chemistry and organic macromolecules

The ubiquitous nanoplastics (NPs) in the environment are emerging contaminants due to their risks to human health and ecosystems. The interaction between NPs and minerals determines the environmental and ecological risks of NPs. In this study, the deposition behaviors of carboxyl modified polystyrene nanoplastics (COOH-PSNPs) with goethite (α-FeOOH) were systematically investigated under various solution chemistry and organic macromolecules (OMs) conditions (i.e., pH, ionic type, humic acid (HA), sodium alginate (SA), and bovine serum albumin (BSA)). The study found that electrostatic interactions dominated the interaction between COOH-PSNPs and goethite. The deposition rates of COOH-PSNPs decreased with an increase in solution pH, due to the enhanced electrostatic repulsion by higher pH. Introducing cations or anions could compress the electrostatic double layers and compete for interaction sites on COOH-PSNPs and goethite, thereby reducing the deposition rates of COOH-PSNPs. The stabilization effects, which were positive with ions valence, followed the orders of NaCl ≈ KCl < CaCl2, NaNO3 ≈ NaCl < Na2SO4 < Na3PO4. Specific adsorption of SO42− or H2PO4− caused a potential reversal of goethite from positive to negative, leading to the electrostatic forces between COOH-PSNPs and goethite changed from attraction to repulsion, and thus significantly decreasing deposition of COOH-PSNPs. Organic macromolecules could markedly inhibit the deposition of COOH-PSNPs with goethite because of enhanced electrostatic repulsion, steric hindrance, and competition of surface binding sites. The ability for inhibiting the deposition of COOH-PSNPs followed the sequence of SA > HA > BSA, which was related to their structure (SA: linear, semi-flexible, HA: globular, semi-rigid, BSA: globular, with protein tertiary structure) and surface charge density (SA > HA > BSA). The results of this study highlight the complexity of the interactions between NPs and minerals under different environments and provide valuable insights in understanding transport mechanisms and environmental fate of nanoplastics in aquatic environments.

Cationic nanoplastic causes mitochondrial dysfunction in neural progenitor cells and impairs hippocampal neurogenesis

Nanoplastics (NPs) exposure to humans can occur through various routes, including the food chain, drinking water, skin contact, and respiration. NPs are plastics with a diameter of less than 100 nm and have the potential to accumulate in tissues, leading to toxic effects. This study aimed to investigate the neurotoxicity of polystyrene NPs on neural progenitor cells (NPCs) and hippocampal neurogenesis in a rodent model. Toxicity screening of polystyrene NPs based on their charge revealed that cationic amine-modified polystyrene (PS–NH3+) exhibited cytotoxicity, while anionic carboxylate-modified polystyrene (PS–COO-) and neutral NPs (PS) did not. NPCs treated with PS-NH3+ showed a significant reduction in growth rate due to G1 cell cycle arrest. PS-NH3+ increased the expression of cell cycle arrest markers p21 and p27, while decreasing cyclin D expression in NPCs. Interestingly, PS-NH3+ accumulated in mitochondria, leading to mitochondrial dysfunction and energy depletion, which caused G1 cell cycle arrest. Prolonged exposure to PS-NH3+ in C17.2 NPCs increased the expression of p16 and senescence-associated secretory phenotype factors, indicating cellular senescence. In vivo studies using C57BL/6 mice demonstrated impaired hippocampal neurogenesis and memory retention after 10 days of PS-NH3+ administration. This study suggests that NPs could deplete neural stem cell pools in the brain by mitochondrial dysfunction, thereby adversely affecting hippocampal neurogenesis and neurocognitive functions.

Co-transport behavior and Trojan-horse effect of colloidal microplastics with different functional groups and heavy metals in porous media

The emerging global problems of microplastics pollution and their co-occurrence with other pollutants have presented major new challenges for environmental health and protection. This study used column experiments to investigate the co-transport behavior and Trojan-horse effect of colloidal microplastics (non-functional polystyrene microspheres (MS), carboxyl-modified polystyrene microspheres (CMS) and sulfonate-modified polystyrene microspheres (SMS)) and lead (Pb) in porous media. Results showed that a Trojan-horse effect occurred during the co-transport of colloidal microplastics and Pb. In the process of co-transport, colloidal microplastics and Pb mutually inhibited each other’s transport at an ionic strength of 1 mM, which may be due to Pb absorption by microplastics, resulting in the destabilization of agglomerates and a reduction in the electronegativity of microplastics. At an ionic strength of 100 mM, colloidal microplastics and Pb promoted each other’s transport, potentially due to their competition for adsorption in porous media. The functional groups present on colloidal microplastics inhibited the transport of Pb at low ionic strengths, while at high ionic strengths Pb transport was promoted. Furthermore, deposition experiments verified that quartz crystal microbalance with dissipation (QCM-D) monitoring could effectively account for and predict the transport and deposition behavior of microplastics in the presence or absence of Pb.

Combined effects of microplastics and excess boron on Microcystis aeruginosa

Both microplastics (MPs) and excess boron (B) have adverse effects on microalgae. However, the combined toxic effects of MPs and excess B on microalgae have not been studied. The aim of this study was to investigate the combined effects of excess B and three types of surface-modified microplastics, including plain polystyrene (PS-Plain), amino-modified polystyrene (PS-NH2), and carboxyl-modified polystyrene (PS-COOH), on chlorophyll a content, oxidative damage, photosynthetic activity and microcystin (MC) production in Microcystis aeruginosa. The results showed that PS-NH2 inhibited the growth of M. aeruginosa, with the maximum inhibition rate of 18.84 %, while PS-COOH and PS-Plain showed stimulatory effects with the maximum inhibition rates of −2.56 % and − 8.03 % respectively. PS-NH2 aggravated the inhibition effects of B, while PS-COOH and PS-Plain alleviated the inhibition effects. Furthermore, the combined exposure of PS-NH2 and excess B had a much greater effect on oxidative damage, cell structure, and production of MCs in algal cells than the combined effects of PS-COOH and PS-Plain. The charges on microplastics affected both B adsorption on microplastics and the aggregation of microplastics with algal cells, indicating that the charge on microplastics is a dominant factor influencing the combined effects of microplastics and excess B on microalgae. Our findings can provide direct evidence for the combined effects of microplastics and B on freshwater algae and improve the understanding of the potential risks of microplastics in aquatic ecosystems.

Transfer of Polystyrene Microplastics with Different Functional Groups in the Aquatic Food Chain

Microplastic pollution has become an increasingly concerning environmental problem because of its adverse impact on aquatic ecosystem security. Microplastics in the aquatic environment are poisonous to aquatic life, and they can not only be consumed by aquatic life but also pass down the food chain, endangering human health in the process. To investigate the accumulation and transfer of three different functional microplastics, including polystyrene (PS), carboxylate-modified polystyrene (PS-COOH), and amine-modified polystyrene (PS-NH2), in the aquatic food chain between Chlorella pyrenoidosa,Daphnia magna, and zebrafish, this study focused on three different functional microplastics. The maximum concentration of the three microplastics in Daphnia magna occurred at 8h, while that in zebrafish appeared at 24h. Three microplastics accumulated in the following order: PS-COOH > PS-NH2 > PS. The BMF of zebrafish intestine/Daphnia magna > 1, suggesting that biomagnification occurred along the food chain transfer and mainly accumulated in the intestine of zebrafish. This study confirmed the biomagnification of three polystyrene microplastics during food chain transfer, providing a theoretical basis for the transfer of microplastics in aquatic food chains.

Solution composition dependent Soret coefficient using commercial MicroScale Thermophoresis instrument.

Thermal diffusion of particles in dilute aqueous suspensions is driven by the interactions between the dispersing medium and the particle, which are largely influenced by the properties of the medium. Using a commercial instrument to generate thermophoresis, we developed a method to quantify the migration of colloids in a temperature gradient and further studied how it varies based on the composition and pH of the dispersing medium and with an anionic surfactant, at different salt concentrations. Thermophoretic migration of aqueous suspensions of carboxylate-modified polystyrene particles with different compositions is measured as MicroScale Thermophoresis (MST) traces and a mathematical model is developed to extract the Soret coefficient (ST). Soret coefficient measurements obtained using the developed method are in-line with previous theories and scientific findings from other literature, indicating a dependence of the ST on the Debye length and surface charge density of the suspended particles, both of which are controlled by the composition of the dispersing medium. The thermophobic/thermophilic behavior of particles is also found to be strongly influenced by the thermoelectric effect of the buffer ions. In this paper, a new analytical model is introduced and applied to complex systems to understand their thermophoretic behavior as a function of solvent properties.

Removal of nanoplastics from aqueous solution by aggregation using reusable magnetic biochar modified with cetyltrimethylammonium bromide

Nanoplastics (NPs) pollution has become an emerging threat to the aquatic environment and its organisms. The removal of NPs from contaminated water is a global challenge. In this study, an efficient and reusable composite was prepared from cetyltrimethylammonium bromide (CTAB) modified magnetic biochar. The performances of CTAB modified magnetic biochar (CMB) to remove polystyrene (PS) and carboxylate-modified polystyrene (CPS) nanoparticles from water were systematically evaluated. The results showed that the PS and CPS removal performance of magnetic biochar was improved by CTAB modification. These increases were assigned to the increase in the surface hydrophobicity of CMB. Due to the strong electrostatic repulsion between the nanoparticles, PS and CPS maintained high stability in alkaline conditions, resulting in a significant decrease in removal efficiency. The removal efficiency was decreased to 67.4% for PS and to 40.7% for CPS at pH 11. The inhibition effects of NaCl on the PS and CPS removal efficiencies were decreased gradually with the increase of NaCl concentration. Among the anions studied, H2PO4− had the biggest impact on the removal performance of CMB. Besides, CMB could be used to remove PS and CPS in real surface water, and the removal efficiencies of PS and CPS were 95.3% and 97.8%, respectively. Particularly, the removal efficiencies of PS and CPS were 90.2% for PS and 94.8% for CPS when CMB was recycled five times. According to the characterization results of XRD, TGA, SEM, FTIR and XPS, PS and CPS nanoparticles were removed by CMB from water mainly through aggregation instead of adsorption. The efficient removal of PS and CPS by CMB via aggregation process offers new insight into the removal of NPs from aquatic environment.

Carboxyl-modified polystyrene microplastics induces neurotoxicity by affecting dopamine, glutamate, serotonin, and GABA neurotransmission in Caenorhabditis elegans

Microplastics (MPs) are ubiquitous in various environmental media and have potential toxicity. However, the neurotoxicity of carboxyl-modified polystyrene microplastics (PS-COOH) and their mechanisms remain unclear. In this study, Caenorhabditis elegans was used as a model to examine the neurotoxicity of polystyrene microplastic (PS) and PS-COOH concentrations ranging from 0.1 to 100 μg/L. Locomotion behavior, neuron development, neurotransmitter level, and neurotransmitter-related gene expression were selected as assessment endpoints. Exposure to low concentrations (1 μg/L) of PS-COOH caused more severe neurotoxicity than exposure to pristine PS. In transgenic nematodes, exposure to PS-COOH at 10–100 μg/L significantly increased the fluorescence intensity of dopaminergic, glutamatergic, serotonergic, and aminobutyric acid (GABA)ergic neurons compared to that of the control. Further studies showed that exposure to 100 μg/L PS-COOH can significantly affect the levels of glutamate, serotonin, dopamine, and GABA in nematodes. Likewise, in the present study, the expression of genes involved in neurotransmission was altered in worms. These results suggest that PS-COOH exerts neurotoxicity by affecting neurotransmission of dopamine, glutamate, serotonin, and GABA. This study provides new insights into the underlying mechanisms and potential risks associated with PS-COOH.

Enhanced removal of aged and differently functionalized polystyrene nanoplastics using ball-milled magnetic pinewood biochars

In this study, simple and environmentally friendly magnetic biochars were successfully prepared by ball-milling biochar with Fe3O4 nanoparticles to remove NPs from water. The magnetic biochars synthesized at various pyrolysis temperatures of 300 °C (MBC300), 500 °C (MBC500), and 700 °C (MBC700) were used to eliminate the unmodified (PS), aged under UV radiation (UVPS), amine-modified (PS–NH2) and carboxylate-modified (PS–COOH) polystyrene NPs of 100 nm in size. Results showed that the removal efficiency of MBC300, MBC500, and MBC700 for PS were 43.67, 82.73 and 57.02%, which were 3.01, 5.76, and 3.10 times greater than that of corresponding pristine biochars at the same temperatures, respectively, and the strongest removal efficiency of MBC500 was 95.2% since it has the largest specific surface area and abundant oxygen-containing functional groups. The surface properties of the NPs affected their removal, and the PS-NH2 had the highest removal rate using magnetic biochars. Compared to pristine biochars, the magnetic biochars displayed faster adsorption kinetics. The Langmuir maximum adsorption capacity of magnetic biochars for NPs were 107.7181–229.5772 mg/g, much greater than those of the pristine biochars (55.4602–80.3096 mg/g). Mechanism analysis revealed that the hydrophobicity, electrostatic attraction, H-bonding formation and π-π conjunction between the NPs and MBCs contributed to the adsorption process. This work highlights the promising potential of ball milling to be used as a simple technique for the preparation of magnetic biochar to remove NPs, especially NPs with various surface groups.

Classification and characterization of immune haemocytes in the larvae of the Indian fritillary, Papilio hyperbius (Lepidopetra: Nymphalidae)

In this study, haemocytes present in Papilio hyperbius Linnaeus were identified and characterized. Six different types of haemocyte were recorded in the haemocoel of this species of insect: prohaemocytes, plasmatocytes, granulocytes, spherulocytes, adipohaemocytes and oenocytoids. Of these the granulocytes were found to be responsible for cell-mediated immune responses such as phagocytosis. Granulocytes that were exposed to immunity inducers (carboxylate-modified polystyrene latex beads [CLBs] and Escherichia coli) had fan-like or pod-like structures on their cell membranes. The lysosomes in granulocytes were activated 2 h after injection with E. coli and after 12 h, all granulocytes exhibited highly activated lysosomes. After 24 and 48 h, the lysosome activity in granulocytes decreased. Transmission electron microscopy revealed that phagocytosis, which was mediated by granulocytes in the early hours of the E. coli infection, led to the formation of one phagosome for one E. coli within the cytosol. Moreover, as time passed, endosomes or lysosomes of different size developed. Subsequently, the phagosomes and lysosomes fused and E. coli were eliminated. After this series of immune responses, the nuclei of the granulocytes were indistinct and their cellular activity decreased. Hence, as old immune cells were replaced by new ones, active and healthy immune haemocytes were presumed to be maintained in the hemocoel.

Insights into the interaction mechanism of ofloxacin and functionalized nano-polystyrene

Nano-plastics (NPs), an emerging contaminant in the environment, have a larger specific surface area and can act as a carrier of other contaminants. Thus, insights into the interaction mechanisms between NPs and other pollutants are crucial for the assessment of environmental impacts of NPs in the ecosystems. In this study, the interaction mechanism between NPs and ofloxacin (OFL) were investigated via kinetics, fluorescence quenching, and two-dimensional correlation spectroscopy (2DCOS). The adsorption kinetics of OFL on carboxyl-modified polystyrene (PS-COOH) and amine modified polystyrene (PS-NH2) closely fitted the pseudo-second-order kinetics model (R2 = 0.99). Adsorption kinetics indicated that chemical adsorption is dominant mechanism, and the Fourier Transform Infrared Spectrometer (FT-IR) and X-ray photoelectron spectroscopy (XPS) results showed that the electronic interaction, π-π, and H-binding were also involved in the adsorption process. OFL showed strong fluorescence quenching in the presence of NPs. Stern-Volmer quenching was negatively related with the temperature, which was dominated by the static type of quenching. 2DCOS indicated that the π-π conjugation was dominant in the interaction process, and the interaction process was dependent on the solution pH and salinity. Overall, this work provides new insights into the interaction mechanism of NPs and antibiotics in the aquatic ecosystems.

Aqueous aggregation and deposition kinetics of fresh and carboxyl-modified nanoplastics in the presence of divalent heavy metals

The presence of heavy metals alters the colloidal stability and deposition of nanoplastics (NPs) in urban waters. Such processes are important to assess the mobility and fate of NPs and their associated heavy metals. Up to date, few studies have reported the impact of heavy metals on the colloidal behaviors of NPs and the involved mechanisms. In the study, time-resolved dynamic light scattering (DLS) and quartz crystal microbalance with dissipation (QCM-D) methods were used to assess the aggregation and deposition kinetics of polystyrene nanospheres with divalent heavy metals. For comparison, carboxyl-modified polystyrene nanospheres were used. Results reveal that heavy metals destabilized NPs more significantly than calcium ions. Spectroscopy and transmission electron microscopy analysis propose that heavy metals destabilized NPs via inner-sphere coordination with carboxyl groups and cation-π interactions, further leading to the formation of different dimensional aggregates. QCM-D results suggest that the deposition rate, irreversibility, and film compactness of NPs on silica surfaces first increased but further decreased as heavy metal concentration increased. Such deposition behaviors depended on the bridging effects between NPs and silica and aggregation-induced diffusion limitation. In that case, the destabilization and retention ability of heavy metals for NPs were related to their electronegativity and hydration shell thickness. In urban waters, the presence of natural organic matter (NOM) decreased the destabilization and retention ability of heavy metals, whereas heavy metals with environmentally relevant concentrations still enhanced the aggregation and deposition of NPs compared with other environmental cations. This study highlights the impact of heavy metal property on the colloidal behaviors of NPs, thus deepening our understanding of the mobility and fate of NPs associated with heavy metals in urban waters.

P-257 Nanosphere-based assessment of zona pellucida permeability in humans and mammalian models

Abstract Study question What is the maximum particle size that can traverse through human zona pellucida (ZP) and that of other mammals? Summary answer Human oocytes ZP is impermeable to 40-100 nm nanospheres. ZP of other mammals is impermeable to 100 nm particles, and permeability changes along preimplantation development. What is known already ZP is a porous structure that protects oocytes and early embryos, being permeable to nourishing and signaling substances while blocking the entrance of immune cells and viruses into the perivitelline space. Size-dependent ZP permeability is poorly known and may differ between species, as in humans and rabbits ZP it is composed by 4 proteins (ZP1-4), while bovine and murine ZPs contain only 3 proteins (ZP2-4 in bovine, ZP1-3 in mice). Understanding permeability is crucial to determine embryo susceptibility to viral infections or large biological particles such as exosomes, and to assess possible damages inferred by artificial reproductive techniques. Study design, size, duration The objective has been to determine ZP permeability in mice, bovine, rabbit (wild-type or lacking ZP4) or human oocytes and/or embryos based on particle size. The assay was performed in 3 replicates of 3 samples per stage: mouse samples (oocytes, zygotes, 2-cell embryos and blastocysts) collected in vivo, bovine samples (oocytes, zygotes, 4-8 cell embryos and blastocysts) produced in vitro, rabbit samples (zygotes) collected in vivo and immature human oocytes discarded for clinical use. Participants/materials, setting, methods Permeability test consisted of incubating oocytes and embryos in 0.005 % solutions of carboxylate-modified polystyrene nanospheres (20, 40 and 100 nm) loaded with a fluorophore emitting at a 505/515 nm for 30 min. Following incubation and washing, samples were observed under a structured illumination fluorescence microscope to determine if particles are present inside ZP (i.e., ZP is permeable to that size of particle) or fluorescence cannot be detected (i.e., particles unable to traverse ZP). Main results and the role of chance ZPs of all species and stages blocked efficiently the pass of 100 nm-sized nanospheres. 40 mm nanospheres were blocked by the ZP of oocytes and zygotes in all species with the exception of rabbit zygotes lacking ZP4. ZP permeability changed during early development, as ZP from murine and bovine cleaved embryos and blastocyst were permeable to 40 nm-sized particles. Finally, with the exception or WT rabbit zygotes, ZP from all oocytes and embryos analyzed were permeable to 20 nm nanospheres. As a fluorescence control, murine and bovine oocytes without ZP were exposed to nanospheres of all sizes, which were clearly visualized attached to the oolema or into the ooplasm. Limitations, reasons for caution Experiments on cleavage and blastocysts stages were performed on animal models, so whether human ZP permeability changes following fertilization remains unknown. Wider implications of the findings Mammalian ZP acts as a selective barrier during early embryo development. Understanding particle size-dependent permeability is crucial to determine embryo susceptibility to viral infection or to signaling mediated by large particles such as extracellular vesicles and exosomes and to assess the impact of oocyte manipulation techniques. Trial registration number Not applicable

Effects of polystyrene nanoplastics with different functional groups on rice (Oryza sativa L.) seedlings: Combined transcriptome, enzymology, and physiology

Plastic particles originate from the daily use of plastics and have become a new form of pollutant. However, the effects of nanoplastics (NPs) on higher plants remain largely unclear, thus requiring further research. In this study, rice plants were exposed to polystyrene (PS) NPs with different functional groups to determine their toxicity. The presence of NPs reduced the biomass and photosynthetic capacity of rice. Compared with control (CK), the heights of rice plants exposed to no-modified PS, carboxyl-modified PS (PS-COOH) and amino-modified PS (PS-NH2) groups decreased by 13.59%, 26.61%, and 42.71%, while the dry shoot weight decreased by 47.46%, 50.09%, and 71.04%, respectively. All treatments activated the antioxidant levels of rice and reduced photosynthesis. Transcriptome analysis showed that NPs induced the expression of genes related to antioxidant enzyme activity in rice roots. Rice could partially reduce the xenobiotic toxicity caused by external sources by regulating phenylpropane biosynthesis and the processes involved in cell detoxification. PS mainly affected the process of RNA metabolism, while PS-COOH mainly affected ion transport, and PS-NH2 mainly affected the synthesis of macromolecular protein, which had different effects on rice growth.

ROS-mediated photoaging pathways of nano- and micro-plastic particles under UV irradiation

Reactive oxygen species (ROS) generation is considered as an important photoaging mechanism of microplastics (MPs) and nanoplastics (NPs). To elucidate the ROS-induced MP/NP aging processes in water under UV365 irradiation, we examined the effects of surface coatings, polymer types and grain sizes on ROS generation and photoaging intermediates. Bare polystyrene (PS) NPs generated hydroxyl radicals (•OH) and singlet oxygen (1O2), while coated PS NPs (carboxyl-modified PS (PS-COOH), amino-modified PS (PS-NH2)) and PS MPs generated fewer ROS due to coating scavenging or size effects. Polypropylene, polyethylene, polyvinyl chloride, polyethylene terephthalate and polycarbonate MPs only generated •OH. For aromatic polymers, •OH addition preferentially occurred at benzene rings to form monohydroxy polymers. Excess •OH resulted in H abstraction, CC scission and phenyl ring opening to generate aliphatic ketones, esters, aldehydes, and aromatic ketones. For coated PS NPs, •OH preferentially attacked the surface coatings to result in decarboxylation and deamination reactions. For aliphatic polymers, •OH attack resulted in the formation of carbonyl groups from peracid, aldehyde or ketone via H abstraction and CC scission. Moreover, 1O2 might participate in phenyl ring opening for PS NPs and coating degradation for coated PS NPs. This study facilitates understanding the ROS-induced weathering process of NPs/MPs in water under UV irradiation.