Carbon Dioxide Treatment Research Articles

Influence of Microwave-Assisted Supercritical Carbon Dioxide Treatment on the Pore Structure of Low-Rank Coal

Influence of Microwave-Assisted Supercritical Carbon Dioxide Treatment on the Pore Structure of Low-Rank Coal

Analysis of Corrosion Causes and Optimization of Corrosion Evaluation Methods for Water Injection System in Changqing JY Oilfield

Abstract Based on the corrosion characteristics of the water injection system in Changqing Oilfield, an experimental study was conducted on the corrosion of the water injection system caused by carbon dioxide, hydrogen sulfide, bacteria, and water treatment agents in the produced liquid. The main influencing factors of corrosion in the water injection system were analyzed, and it was clarified that the corrosion of the water injection system in Changqing Oilfield was mainly caused by oxygen corrosion, which also had an impact on the water quality of the injection. Combining static corrosion analysis with the introduction of pitting and dynamic corrosion rate testing methods, the average corrosion rate of JY oilfield water injection system has a positive correlation with pitting corrosion, while the average corrosion rate of clean water has a stronger correlation with pitting corrosion. The corrosion evaluation method has been optimized, providing technical basis for formulating effective anti-corrosion measures for the oilfield.

Novel proton-conducting hexagonal perovskites Ba7In6–xYxAl2O19 for solid oxide fuel cells

The solid solution Ba7In6–xYxAl2O19 (0≤x≤0.25) with a hexagonal perovskite-like structure was prepared by the solid-state route. The introduction of yttrium was accompanied by an increase in lattice parameters. The total conductivity as a function of pO2 and T was measured at different humidity. The partial conductivities were found by transport number measurements and were fitted based on the defect formation model. Oxygen-ion and proton conductivities were found to increase with Y3+ content as a result of an increase in cell volume and free volume, which was accompanied by a decrease in the activation energy of both oxygen-ion and proton conductivity. Oxygen-ion conductivity and proton conductivity dominated below 500 °C in dry (pH2O=3.5×10−5 atm) and in wet (pH2O=1.92×10−2 atm) conditions, respectively. Doping makes it possible to increase proton conductivity by an order of magnitude (500 °C) and significantly increase the proton transport numbers.The prepared phases exhibited excellent chemical stability during thermal treatment in carbon dioxide (pCO2=1 atm).

Gas encapsulation technology for large volume press

Abstract For samples in the gaseous state at room temperature and pressure, mature technology has been developed, which can be encapsulated in a diamond anvil cell (DAC). However, large volume press (LVP) can only treat samples with starting materials in solid or liquid form. We have achieved stable encapsulation and reaction treatment of carbon dioxide in centimeter sized sample chamber for a long time (over 10 minutes) under conditions of temperature higher than 1200 ℃ and pressure over 5 GPa through the use of integrated low-temperature freezing and rapid compression sealing method for LVP cell assemblies. This technology can also be applied to the packaging of other gaseous or liquid samples, such as ammonia, sulfur dioxide, water, etc., in LVP devices.

Effect of supercritical carbon dioxide on protein structure modification and antimicrobial peptides production of Mongolian cheese and its in vitro digestion

The aim of this research was to investigate the effects of supercritical carbon dioxide (SC-CO2) treatment on protein structure in Mongolian cheese. The peptides during the digestive process of the SC-CO2 treated cheese were also studied. SC-CO2 technology was utilized to treat Mongolian cheese at three temperatures (45, 55 and 65 °C) and three pressures (7.5, 12.5 and 17.5 MPa). The results of fluorescence, ultraviolet–visible, Fourier transform infrared spectroscopy and free sulfhydryl groups showed that SC-CO2, particularly at 65 °C and 17.5 MPa, modified the protein structure in Mongolian cheese effectively. The data of LC-MS/MS-based peptidomics showed that the content of antimicrobial peptides found in the SC-CO2 treated Mongolian cheese was 1.55 times that of the untreated Mongolian cheese; the content of unique antimicrobial peptides in the digested SC-CO2 treated Mongolian cheese was 1.46 times that of the digested untreated Mongolian cheese, which proved that SC-CO2 could help produce antimicrobial peptides in cheese not only during the process of SC-CO2 treatment but during subsequent simulated gastrointestinal digestion as well. In conclusion, SC-CO2 could be considered a promising method to develop cheese products with potential health benefits.

First Data on the (Poly)phenolic Profiling of Farmacista Honorati Persimmon Fruit (Diospyros kaki Thunb.) at Commercial Harvest and after Treatments for Astringency Removal.

This study aims to provide the first report on the soluble and polyphenolic profiles of "Farmacista Honorati" (FH) persimmons, which is a marketed cultivar with no existing data on its nutraceutical value. Total soluble tannins (TSTs) and major soluble (poly)phenols in FH fruits before and after post-harvest commercial treatments with carbon dioxide and ethylene were analyzed. Fruits at commercial harvest had a TST content of 1022 ± 286 mg GAL/100 g d.w. Whereas, after deastringency treatments, an 85% and 83% reduction were observed for carbon dioxide- and ethylene-treated fruits, respectively. Carbon dioxide treatment resulted in the insolubilization of tannins around comparable values in most fruit cultivars, despite the variable soluble tannin content in untreated fruit. By targeted metabolomic profiling, nineteen (poly)phenolic substances were quantified in the investigated untreated and treated fruits. Gallic acid (99 mg/100 g d.w.), (+)-catechin (1.8 mg/100 g d.w.), ellagic acid (1.2 mg/100 g d.w.), and (-)-epicatechin (1.1 mg/100 g d.w.) were the predominant compounds in the untreated FH samples. After the application of post-harvest treatments, a non-nutraceutical relevant decrease of 8-19% in the targeted (poly)phenolic content was generally observed. Ethylene induced the most significant reduction in the individual (poly)phenolic compounds in the FH fruits.

Hydrophobic Silk Fibroin-Agarose Composite Aerogel Fibers with Elasticity for Thermal Insulation Applications.

Aerogel fibers, characterized by their ultra-low density and ultra-low thermal conductivity, are an ideal candidate for personal thermal management as they hold the potential to effectively reduce the energy consumption of room heating and significantly contribute to energy conservation. However, most aerogel fibers have weak mechanical properties or require complex manufacturing processes. In this study, simple continuous silk fibroin-agarose composite aerogel fibers (SCAFs) were prepared by mixing agarose with silk fibroin through wet spinning and rapid gelation, followed by solvent replacement and supercritical carbon dioxide treatment. Among them, the rapid gelation of the SCAFs was achieved using agarose physical methods with heat-reversible gel properties, simplifying the preparation process. Hydrophobic silk fibroin-agarose composite aerogel fibers (HSCAFs) were prepared using a simple chemical vapor deposition (CVD) method. After CVD, the HSCAFs' gel skeletons were uniformly coated with a silica layer containing methyl groups, endowing them with outstanding radial elasticity. Moreover, the HSCAFs exhibited low density (≤0.153 g/cm3), a large specific surface area (≥254.0 m2/g), high porosity (91.1-94.7%), and excellent hydrophobicity (a water contact angle of 136.8°). More importantly, they showed excellent thermal insulation performance in low-temperature (-60 °C) or high-temperature (140 °C) environments. The designed HSCAFs may provide a new approach for the preparation of high-performance aerogel fibers for personal thermal management.

Widely targeted metabolomics analysis reveals the potential metabolic network of high-pressure carbon dioxide regulating fresh-cut Chinese water chestnut yellowing

In this study, we applied widely targeted metabolomics and physiological analysis to investigate the non-volatile metabolites changes and regulatory mechanisms of high-pressure carbon dioxide (HPCD) treatment on the yellowing of fresh-cut Chinese water chestnut (CWC). A total of 349 non-volatile metabolites were identified and subjected to principal component analysis (PCA), OPLS-DA and KEGG enrichment analysis. The results showed that the yellowing of fresh-cut CWC was related to membrane lipid metabolism and flavonoid biosynthesis. Damage stress induced membrane lipid metabolism and flavonoid biosynthesis in surface tissues, and the increase of phenylalanine ammonia-lyase (PAL) activity in flavonoid biosynthesis might be triggered by signaling molecules from membrane lipid metabolism. On the one hand, HPCD treatment could directly inhibit the activity of PAL, and reduce the biosynthesis of flavonoids in the damaged tissues. On the other hand, HPCD could indirectly inhibit the generation and accumulation of potential signaling molecules by regulating membrane lipid metabolism, thus preventing signaling molecules from triggering flavonoid biosynthesis. This study proposed a potential network of HPCD regulating the yellowing of fresh-cut CWC and established the foundation for further control of yellowing in fresh-cut CWC.

Insight into the allergenicity and structure changes of parvalbumin from Trachinotus ovatus induced by dense-phase carbon dioxide

Parvalbumin (PV) is a major allergen in fish, and traditional treatments cannot reduce its sensitization. The effects of dense-phase carbon dioxide (DPCD) treatment on the sensitization and spatial structure of PV in Trachinotus ovatus were evaluated in this study. Western blotting and indirect ELISA were used to determine the allergenicity changes and spatial conformations of PV treated by DPCD. Tris-tricine-SDS-PAGE, circular dichroism, surface hydrophobicity, endogenous fluorescence, UV spectrophotometry, free amino group, total sulfhydryl group and SEM analyses were applied to characterize PV structure. The results showed that DPCD treatment (15 MPa, 30 min, 50 °C) could reduce PV-induced allergic reactions by 39–41 %, which destroyed the normal conformational epitopes and reduced the risk of PV-induced allergy. The secondary structure changed from ordered to disordered with a decreased content of α-helical groups, while the internal hydrophobic groups were exposed. The total sulfhydryl group content decreased significantly (P < 0.05). The surface hydrophobicity and ultraviolet absorption spectrum were enhanced, and the endogenous fluorescence peak shifted to a long wavelength. Meanwhile, the content of free amino groups increased significantly (P < 0.05). This study could provide a theoretical basis and a promising technical approach for reduction of PV allergenicities.

Cooperative Operation of Distributed Energy Resources and Thermal Power Plant With a Carbon-Capture-Utilization-and-Storage System

Carbon-capture-utilization-and-storage (CCUS) system plays a critical role in the process of decarbonization. This paper proposes a cooperative operation model for a CCUS-based thermal power plant and distributed energy resources. The critical purpose is to achieve a higher profit and flexibility together with a lower carbon emission for the CCUS system under the electricity, carbon, ancillary services, and synthesis fuel markets. First, to enhance the operational flexibility in various markets, a critical focus of this paper is on elaborating feasible operation domain of the CCUS system through identifying the physicochemical processes associated with carbon dioxide (CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) treatment and multi-energy conversion. Second, a Nash-bargaining framework is developed to describe how the CCUS works with distributed energy resources cooperatively to consume cheap and clean renewables to treat CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> . Third, to solve the cooperative operation problem efficiently and accurately, an adaptive sequential cone programming (ASCP) method together with a progressive hedging variant of the Benders decomposition (PH-BD) algorithm are designed. Case studies indicate the total revenue of the thermal power plant under the cooperative mode is improved by up to 8.85% and 66.53% compared with the individual mode with and without the CCUS. Further, the solution quality and efficiency of proposed methods are verified.

ВЛИЯНИЕ ПРИМЕНЕНИЯ ДИОКСИДА УГЛЕРОДА НА КАЧЕСТВО ИКРЫ ЛОСОСЕВОЙ ЗЕРНИСТОЙ

In this paper, we present the results on the effect of carbon dioxide application in the technology of salmon caviar on the quality of finished product. The dependence of the mechanical strength of eggs on the aggre- gate state of carbon dioxide during caviar processing with gaseous CO2 and its solutions at different stages of the technological process is shown. The negative effect of carbon dioxide solutions on the condition of salmon egg’s membrane was concluded, since the membrane strength reduced, which was accompanied by an increase in the manifestation of defects in the burst eggs and, as a result, liquid sediment in the product. The rational duration of carbon dioxide treatment of salmon eggs was determined and it made 25–30 minutes. The directions of further research on caviar CO2 technologies were determined.

Recent progress on CO2 separation membranes.

Presently, excessive carbon dioxide emissions represent a critical environmental challenge. Thus, urgent efforts are required to develop environmentally friendly and low-energy technologies for carbon dioxide treatment. In this case, membrane separation technology stands out as a promising avenue for CO2 separation, with selective membrane materials of high permeability playing a pivotal role in this process. Herein, we categorize CO2 separation membranes into three groups: inorganic membranes, organic membranes, and emerging membranes. Moreover, representative high-performance membranes are introduced and their synthesis methods, gas separation performances, and applications are examined. Furthermore, a brief analysis of the challenges encountered by carbon dioxide separation membrane materials is provided together with a discussion on the future research direction. It is expected that this review will provide some potential insights and guidance for the future development of CO2 separation membranes, which can promote their development.

Quality properties and nutritional compounds of fresh-cut pumpkin treated with supercritical carbon dioxide

The work aimed to explore the effect of supercritical carbon dioxide (SCCD) treatment on the enzyme activity, color, carotenoid, sugar and phenolic profile, and antioxidant activity of fresh-cut pumpkins. The polyphenol oxidase (PPO) and peroxidase (POD) activities of SCCD-treated pumpkin cubes were reduced by 21% and 18%, respectively. The lightness value of SCCD-treated samples declined compared to the untreated samples, whereas the redness and yellowness values in treated samples were enhanced. The lutein, α-carotene, β-carotene and total carotenoids, glucose, sorbitol, and other polysaccharides in the SCCD-treated samples showed a fluctuation trend. Similarly, the treated sample's total phenolic contents (TPC), ABTS (2,2′- azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), and superoxide radical scavenging activities were increased and then reduced. An enhancement was noted in the coumaryloquinic acid I and II, caffeic acid glucoside, 4-hydroxybenzoic acid, and p-coumaric acid of SCCD-treated samples. The work demonstrated that the SCCD technique at moderate conditions could obtain higher retention of nutritional composition in fresh-cut pumpkins.

Carbon dioxide treatment modulates phosphatidic acid signaling and stress response to improve chilling tolerance and postharvest quality in paprika

IntroductionPaprika (Capsicum annuum L.) is prone to chilling injury (CI) during low-temperature storage. Although recent findings suggest that CO2 treatment may protect against CI, the effects of short-term CO2 treatment on CI and the underlying molecular mechanisms in paprika remain unknown. Therefore, this study aimed to examine the effect of short-term CO2 treatment on CI and postharvest quality in paprika during storage at cold storage and retail condition at physio-biochemical-molecular level.MethodsPaprika was treated with 20 and 30% CO2 for 3 h and stored at 4°C for 14 days, followed by additional storage for 2 days at 20°C (retail condition). Fruit quality parameters, including weight loss, firmness, color, and pitting were assessed, and the molecular mechanism of the treatment was elucidated using transcriptomic and metabolomic analyses.ResultsShort-term treatment with 20 and 30% CO2 effectively maintained paprika quality during cold storage and retailer conditions, with reduced surface pitting, a common symptom of CI. Additionally, transcriptomic and metabolomic analyses revealed that 20% CO2 treatment induced genes associated with biosynthesis of phosphatidic acid (PA), diacylglycerol, triacylglycerol, and stress response, metabolites associated with phasphatidyl inositol signaling, inositol phosphate metabolism, and starch and sucrose metabolism.ConclusionCO2 treatment activates PA biosynthesis through PLD and PLC-DGK pathways, and induces inositol phosphate, starch, and sucrose metabolism, thereby regulating chilling stress response via the ICE-CBF pathway. These findings suggest that short-term CO2 treatment enhances resistance to cold-induced injury and preserves postharvest quality in non-climacteric fruits, such as paprika, through activation of PA signaling, which improves membrane stability during cold storage and distribution.

Revealing petroleum-water-CO2 emulsion stability by NMR about oil recovery and carbon storage

CO2 injection can simultaneously enhance oil recovery and carbon storage, but the stability of petroleum-water-CO2 emulsion affcet the production process and carbon storage. Emulsion characteristics is typically assessed via Nuclear Magnetic Resonance (NMR). It is essential to develop a theory to unlock the mechanisms of experimentation for harnessing the full potential of NMR in assessing emulsion stability and comprehending complex multiphase systems. The theory integrates experiments and a “quasi-lattice wave” physical model to understand the microscale mechanisms in NMR measurements of crude oil emulsion properties, and uncover the “transfer-transition” mode of 1H quantum excited states, explaining T2 relaxation. This forms a theoretical basis for numerical simulation of CPMG (Carr-Purcell-Meiboom-Gill) experiments by random walk algorithms and the boundary annihilation hypothesis. A strong link between NMR T2 times and various character properties of Petroleum-Water-CO2 Emulsion samples is reflected, such as environmental scale, viscosity, CO2 adsorption, and molecular thermal motion. Additionally, parameter Θ was proposed to characterize emulsion stability, for penetrating the theory presented in this paper, and to provide a scientifically rigorous representation of crude oil emulsion stability. The experiments with CO2 treatment on crude oil emulsions were performed, demonstrating a highly effective theoretical correlation between interfacial tension in crude oil emulsions and CO2 pressure, with an exceptional fit to experimental data (R2 > 0.99). When the pressure of CO2 treatment exceeds a critical threshold (approximately 17 MPa in this study), there is a substantial reduction in viscosity. Furthermore, a comparative assessment of samples before and after CO2 treatment using the parameter Θ demonstrated its reliability as an indicator of emulsion stability. It revealed a significant reduction of over 50% in the linear fit residual variance of Θ before and after subjecting the sample to 10 MPa carbon dioxide treatment, highlighting improved fluctuation stability. The slope of the Θ linear regression function signifies temporal stability. In a sample with 20% water content, the time evolution of the water phase decreases by 3.04%, while the oil phase experiences a substantial 57.77% reduction. Simultaneously, the midpoint of the range of the Θ linear regression function represents the environmental scale. The water phase environment scale increases by 8.72%, while the oil phase environment scale decreases by 8.74%, highlighting spatial evolution patterns.

Enzymatic digestibility of lignocellulosic wood biomass: Effect of enzyme treatment in supercritical carbon dioxide and biomass pretreatment

Energy and resource intensive mechanical and chemical pretreatment along with the use of hazardous chemicals are major bottlenecks in widespread lignocellulosic biomass utilization. Herein, the study investigated different pretreatment methods on spruce wood namely supercritical CO2 (scCO2) pretreatment, ultrasound-assisted alkaline pretreatment, and acetosolv pulping-alkaline hydrogen peroxide bleaching, to enhance the enzymatic digestibility of wood using optimized enzyme cocktail. Also, the effect of scCO2 pretreatment on enzyme cocktail was investigated after optimizing the concentration and temperature of cellulolytic enzymes. The impact of scCO2 and ultrasound-assisted alkaline pretreatments of wood were insignificant for the enzymatic digestibility, and acetosolv pulping-alkaline hydrogen peroxide bleaching was the most effective pretreatment that showed the release of total reducing sugar yield (TRS) of ∼95.0 wt% of total hydrolyzable sugars (THS) in enzymatic hydrolysis. The optimized enzyme cocktail showed higher yield than individual enzymes with degree of synergism 1.34 among the enzymes, and scCO2 pretreatment of cocktail for 0.5–1.0 h at 10.0–22.0 MPa and 38.0–54.0 °C had insignificant effect on the enzyme's primary and global secondary structure of cocktail and its activity.

Effect of super critical carbon dioxide and alkali treatment on oxygen barrier properties of thermoplastic starch/poly(vinyl alcohol) films

Abstract Sustainable oxygen barrier thermoplastic starch (TPS)/polyvinyl alcohol (PVA) blown films were successfully prepared by blending proper PVA loads during NaOH-treating and supercritical carbon dioxide (scCO2) assisted processing. The NaOH-treated TPS or scCO2TPS films showed smaller free-volume-cavity characteristics (FVCC) and oxygen transmission rate (OTR) than those of TPS (or scCO2TPS) prepared without proper alkali-treatment. Smaller OTR and FVCC values were detected for NaOH-treated scCO2TPS films than those of NaOH-treated TPS films prepared without scCO2-assistance. All OTR and FVCC values detected for proper alkali-treated scCO2TPS y PVA z films diminished distinctly to a smallest value, when their PVA loads came near a solubility limit value of 27.5 wt%. An essential result is that the OTR of the optimal NaOH-treated scCO2TPS y PVA z film is merely 3.1 cm3/m2 day atm, which meets the requirement of high oxygen barrier plastics. Dynamic molecular relaxations and WAXD patterns detected for proper NaOH-treated scCO2TPS y PVA z films disclosed that PVA was compatible with TPS, as PVA loads were ≤ the solubility limit value. The distinctly reduced OTR and FVCC detected for optimal NaOH-treated scCO2TPS y PVA z films are partially attributed to the reinforced molecular interactions between hydroxyl groups of TPS and PVA, as they were blended with proper PVA loads during their alkali-treating and scCO2-aid processing.

Mangrove peat and algae leachates elicit rapid and contrasting molecular and microbial responses in coastal waters

As sea level rises, previously sequestered blue carbon can be exported offshore as particulate or dissolved organic matter where it may be re-mineralized or sequestered. The priming effect, or interactive effects of organic matter turnover with a mixed substrate, is well described in soils, but still debated in aquatic systems. Priming may contribute to enhanced blue carbon re-mineralization in coastal environments. Here we examined mangrove-derived dissolved organic matter turnover in a lab incubation, with leachates from mangrove peat, 13C-labeled algae, and peat+algae (primed). Particulate and dissolved organic matter were assessed; microbial metatranscriptomes were evaluated; and dissolved organic matter was characterized with high resolution mass spectrometry. Stable isotopes indicated rapid allocation of algal-derived dissolved organic matter into particulate organic matter. The algal treatment had the greatest increase in carbon dioxide, but primed and peat treatments had the greatest loss of dissolved organic carbon, greater RNA concentrations, and similar changes in total carbon dioxide. This suggests that, while total carbon dioxide did not increase under priming conditions, the addition of a peat substrate may promote microbial biomass production relative to carbon dioxide production. This work highlights that more targeted studies investigating the specific mechanisms of priming are necessary to address the molecular and microbial transformations associated with priming in aquatic systems.

Interlayer adhesion strength of 3D-printed cement-based materials exposed to varying curing conditions

This study explores the influences of the curing regime on the interlayer adhesion properties of 3D printed cement (3DPC) paste. Phase compositions, pore structure, and microstructure of typical specimens were obtained by XRD, TG, MIP, SEM and BSE analyses. The results show that the bond strength of the 3DPC sample is decreased by air curing (AC) and stream curing (SC), compared with constant standard curing (CSC) specimens. In contrast, CO2 curing (CC) results in a good enhancement on the interlayer adhesion strength of the 3DPC matrix. The high plastic viscosity behaves as an improvement on the adhesion properties of the printed specimens with the similar level fluidity. Either air curing or stream curing degraded the adhesion strength of the printed mixture owing to the influence of hydration and form a looser microstructure with high porosity in the micro interface of printed pastes. The decreased bond strength of printed samples induced by relatively low humidity can be alleviated by carbonation curing. Therefore, the sequestration of carbon dioxide treatment provides a potential method for pursuing higher bond strength and lower environmental impact to manufacture 3DPC cement.

Effect of Calcium Chloride (CaCl2) and Carbon Dioxide (CO2) on Post Harvest Quality of Apple Fruit (Malus domestica) cv. Gala

The study investigated the effects of calcium chloride (CaCl2) and carbon dioxide (CO2) on the post-harvest quality of Gala apple fruit (Malus domestica). The apples were stored under controlled refrigerated conditions for 90 days, and eight different treatment combinations were tested: 0.5% CaCl2, 1.0% CaCl2, 900ppm CO2, 1000ppm CO2, 0.5% CaCl2 + 900ppm CO2, 0.5% CaCl2 + 1000ppm CO2, 1.0% CaCl2 + 900ppm CO2, and 1.0% CaCl2 + 1000ppm CO2. Various quantitative and quality parameters were evaluated throughout the experiment, including fruit weight loss, firmness, total soluble solids (TSS), pH, titratable acidity (TA), ascorbic acid, and sensory evaluation at 30, 60, and 90 days of post-harvest storage. The results demonstrated that fruit weight loss was significantly in the treatments with calcium and carbon dioxide compared to the control group. Furthermore, the calcium and carbon dioxide treatments led to an increase in pH and total soluble solids, and causing a decrease in firmness, titratable acidity, and ascorbic acid during the 90-day cold storage at 0 to 2°C. The findings also indicated that the calcium and carbon dioxide treatments influenced the production of ethylene to the control group. Overall, the experiment revealed that post-harvest calcium treatments prevented fruit softening and minimized weight losses. The application of 1.0% CaCl2 + 1000ppm CO2 showed superior results compared to other treatments, effectively controlling weight loss, maintaining firmness, and minimizing fruit decay. In conclusion, storing Gala apples under controlled atmosphere conditions with the application of calcium chloride and carbon dioxide successfully inhibited fungal growth, maintained firmness, minimized weight loss, and extended the shelf life compared to the control group. These findings have significant implications for the apple industry, as they provide a means to improve fruit quality and increase the shelf life of apples. Future research may build upon these results to refine treatment conditions, gain a deeper understanding of the underlying mechanisms, and explore the application of these techniques to other apple varieties and different fruits. Ultimately, these experimental findings have the potential to contribute to the development of practical strategies for enhancing the shelf life of apple fruits, ensuring better quality and reduced post-harvest losses.