Abstract

The work aimed to explore the effect of supercritical carbon dioxide (SCCD) treatment on the enzyme activity, color, carotenoid, sugar and phenolic profile, and antioxidant activity of fresh-cut pumpkins. The polyphenol oxidase (PPO) and peroxidase (POD) activities of SCCD-treated pumpkin cubes were reduced by 21% and 18%, respectively. The lightness value of SCCD-treated samples declined compared to the untreated samples, whereas the redness and yellowness values in treated samples were enhanced. The lutein, α-carotene, β-carotene and total carotenoids, glucose, sorbitol, and other polysaccharides in the SCCD-treated samples showed a fluctuation trend. Similarly, the treated sample's total phenolic contents (TPC), ABTS (2,2′- azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, DPPH (2,2-diphenyl-1-picrylhydrazyl), and superoxide radical scavenging activities were increased and then reduced. An enhancement was noted in the coumaryloquinic acid I and II, caffeic acid glucoside, 4-hydroxybenzoic acid, and p-coumaric acid of SCCD-treated samples. The work demonstrated that the SCCD technique at moderate conditions could obtain higher retention of nutritional composition in fresh-cut pumpkins.

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