Captive Settings Research Articles

Addressing nontarget amplification in DNA metabarcoding studies of arthropod-feeding rodents.

High-throughput sequencing approaches have revolutionized how we study animal diets by enabling the detection of dietary components from the metabarcoding of DNA in excrement. Mitochondrial cytochrome oxidase C subunit I (mtCOI) DNA metabarcoding is commonly used to study the diets of arthropod-feeding animals; however, this approach is susceptible to nontarget amplification of the consumer species mtCOI locus. Nontarget amplification is often an unforeseen complication that can drastically reduce the quality and utility of the results generated by high-throughput amplicon sequencing. By interrogating the diets of new world rodents in the genus Neotoma (woodrats) in both natural and captive settings, we demonstrate that nontarget amplification can drastically reduce the total read abundance of detected arthropod taxa in fecal samples and inhibit downstream analyses of dietary diversity and composition metrics. Using the results from these investigations, we offer a guide on how to identify concerns for nontarget amplification when selecting degenerate primers for DNA metabarcoding studies and recommend several approaches that can reduce or eliminate nontarget amplification. Lastly, for the community interested in investigating the diets of arthropod-feeding rodents, we generated a database containing the degree of mismatch between publicly available Rodentia mtCOI sequences and four common universal mtCOI primer sets to be used as a resource for inferring the relative risk of nontarget amplification when designing arthropod metabarcoding studies in rodent systems. This guide will be especially useful for researchers working with consumer species that have not previously been studied.

Social tolerance and interactional opportunities as drivers of gestural redoings in orang-utans.

Communicative repair is a fundamental and universal element of interactive language use. It has been suggested that the persistence and elaboration after communicative breakdown in nonhuman primates constitute two evolutionary building blocks of this capacity, but the conditions favouring it are poorly understood. Because zoo-housed individuals of some species are more social and more terrestrial than in the wild, they should be more likely to show gestural redoings (i.e. both repetition and elaboration) after communicative failure in the coordination of their joint activities. Using a large comparative sample of wild and zoo-housed orang-utans of two different species, we could confirm this prediction for elaboration, the more flexible form of redoings. Specifically, results showed that gestural redoings in general were best predicted by the specific social action context (i.e. social play) and interaction dyad (i.e. beyond mother-offspring), although they were least frequent in captive Bornean orang-utans. For gestural elaboration, we found the expected differences between captive and wild research settings in Borneans, but not in Sumatrans (the more socially tolerant species). Moreover, we found that the effectiveness of elaboration in eliciting responses was higher in Sumatrans, especially the captive ones, whereas effectiveness of mere repetition was influenced by neither species nor setting. We conclude that the socio-ecological environment plays a central role in the emergence of communicative repair strategies in great apes. This article is part of the theme issue 'Revisiting the human 'interaction engine': comparative approaches to social action coordination'.

American alligator (Alligator mississippiensis) weight gain in captivity and implications for captive reptile body condition.

The body condition of an animal is an indicator of health status and is dependent upon many factors, some of which can vary between wild and captive settings. Despite this, there have not been many studies on how captivity affects body condition relative to wild animal populations. This study explores the body condition of captive and wild American alligators (Alligator mississippiensis) because reptiles are frequently overlooked in studies of captive animal health and because alligators are well-represented in captivity. We collected body condition data from 209 captive alligators and 935 wild alligators throughout Florida and southeastern Georgia and compared the relationships between body condition and body length for each group. We found that captive alligators exhibited significantly higher body condition values as they aged, and that this result was driven by the difference between captive and wild males. Body condition values for captive juveniles did not differ from wild juveniles, but they differed when comparing adults. Our results suggest that factors such as diet and movement rates play major roles in determining alligator body condition and that body condition may be an important metric for monitoring captive alligator health, especially for older adult males.

The Older the Better: Infanticide Is Age-Related for Both Victims and Perpetrators in Captive Long-Tailed Macaques.

Simple SummaryIn captive primates, new males have to be introduced regularly to prevent inbreeding. Sometimes, these males commit infanticide, i.e., the killing of young infants. More knowledge of the risk factors that are associated with infanticide may lower the incidence of infanticide during male introductions. We used explanations of infanticide from wild data and the anti-infanticidal strategies of females to predict these risk factors. Next, we tested these factors using demographic data collected on captive long-tailed macaques over a long period. The ages of both infants and new alpha males are important: infants under the age of 215 days are at risk of being killed, and typically young males (≤8 years of age) commit infanticide. Therefore, to lower the risk of infanticide during male introductions in captivity, we advise introducing only males in their prime age (≥9 years of age), preferably in periods with no infants younger than 215 days of age.In wild primates, infanticide is a risk that is especially prevalent when a new male takes over the alpha position. Insight into risk factors related to infanticide may decrease the incidence of infanticide in captivity during male introductions. We investigated several risk factors of infanticide derived from hypotheses explaining infanticide in the wild and tested this in captive long-tailed macaques (Macaca fascicularis) using demographic data spanning a 25.5-year period. Factors that are related to infanticide in the wild explained a large proportion, but not all incidences, of infanticide in captivity. Consistent with the wild data, infants young enough to decrease the interbirth interval (<215 days) were at risk of being killed. In contrast to studies from the wild, infanticidal males were more than 2.5 years younger than non-infanticidal males. This indicates that captive settings can lead to new risks since relatively young males may gain the alpha position, promoting infanticide. Therefore, we propose the adolescent male risk hypothesis as a captive risk factor in which subadult males pose a risk of infanticide. In conclusion, the ages of both males and infants are related to infanticide in captivity and have to be taken into account during male introductions.

Personality assessment of headstart Texas horned lizards (Phrynosoma cornutum) in human care prior to release

The study of animal personality is a growing field that has applications for welfare of animals living in captive settings. We measured personality traits (activity, exploration, and neophobia) in Texas horned lizards (Phrynosoma cornutum) living in human care before they were released to their natal habitat as part of a headstart program. We found evidence of consistent inter-individual differences in activity and exploration, but not neophobia. We also identified a positive correlation between activity and exploration, such that more active lizards were also more likely to explore a novel environment. These results suggest that Texas horned lizards have individual differences in response to their environment, which can inform husbandry decisions. Extensions of this work could also have implications for conservation of Texas horned lizards and for headstart programs focused on reptiles.

My path to primatology: some stories from the field.

In this paper, I summarize the major facets of my 50-year career as a primatologist. I briefly describe the aspects of my upbringing and early education that led me to the study of primate behavior, first in captive settings and, later, in the wild. My research on the Arashiyama West Japanese macaques and my interactions with Japanese primatologists was a formative stage in my career, and I present the background of this international project and how it led to my growing focus on female life history studies. After a couple of failed attempts to establish a long-term study of primates in their native habitats, I began the Santa Rosa Primate Project in Costa Rica in 1983, which focuses mainly on white-faced capuchins, and to some extent on howlers and spider monkeys. The Santa Rosa project has expanded over the past four decades and continues to this day, with the participation of a large team of colleagues, local field assistants and students. I present some of the major findings of our Santa Rosa monkey research in the areas of female reproduction, sexual conflict and conservation of primates in a regenerating tropical dry forest. I also briefly describe how and why I came to develop a sideline of research on gender and science.

Quantification of members of the Mycobacterium chelonae-abscessus complex in lesions of the endangered houston toad (Anaxyrus houstonensis)

Illumina-based 16S rRNA V3 amplicon sequencing of total DNA obtained from soft tissue lesions (joint granulomas) of the endangered Houston toad (Anaxyrus houstonensis) demonstrated that many reads represented members of the actinobacterial Mycobacterium chelonae-abscessus complex. In order to quantify members of this complex in those lesions, we designed three complex-specific primer set/probe combinations (sets I, II and III) targeting variable regions on the 23S rRNA gene for SybrGreen- and Taqman-based quantitative polymerase chain reaction (qPCR). Both SybrGreen- and Taqman-based analyses specifically detected members of the M. chelonae-abscessus complex in lesion samples, with numbers between 104 and 107 cells per 100-mg sample. Values within individual samples were generally comparable between SybrGreen- and Taqman-based detection methods and between all primer set/probe combinations, except for SybrGreen-based analyses of a few samples analyzed with primer set I that used a less specific forward primer. The development of highly specific detection and quantification methods for members of the M. chelonae-abscessus complex in lesion samples can enable group specific tracking of these organisms, particularly in captive or stewardship settings where source and transmission monitoring are valuable tools to husbandry and species conservation.

Personality matters: exploring the relationship between personality and stress physiology in captive African lions

BackgroundConsidering animals as individuals and not as species is becoming increasingly essential to animal welfare management in captive settings. Recent studies on big cat personalities and coping strategies suggest personality can help big cats cope in their surroundings. Yet a large portion of the published literature focuses on understanding either the personality or stress physiology of big cats. Our research shows how integrating an improved understanding of the personality of big cats with stress physiology may enhance welfare, especially for endangered species like African lions. By using a wild cat personality checklist, this study compared the key personality dimensions of 22 African lions with its faecal glucocorticoids and assessed factors influencing their personality and stress physiology.ResultsWe found two reliable personality dimensions for African lions (dominance and agreeableness) and identified key factors (sex, age and location) that may influence their personality. Further, on testing if these factors influenced the stress physiology through variations in glucocorticoid levels, there was no significant difference. However, there was a strong negative association between agreeableness and glucocorticoid levels. These results suggest that the behavioural traits loading positively and higher for agreeableness are associated with lower glucocorticoid stress levels, which may assist a lion to cope with stressors in its surroundings.ConclusionsOur findings highlight this integrated approach of linking personality and stress physiology of big cats can be beneficial for caretakers. For example, during stressful veterinary procedures or in reintroduction programs, recognizing the personality of lions can help in designing or providing them with resources that will alleviate stress. Thus, there is a need for more interdisciplinary approaches that will contribute towards enhancing the individual and overall welfare of big cats.

Postural behavior recognition of captive nocturnal animals based on deep learning: a case study of Bengal slow loris

The precise identification of postural behavior plays a crucial role in evaluation of animal welfare and captive management. Deep learning technology has been widely used in automatic behavior recognition of wild and domestic fauna species. The Asian slow loris is a group of small, nocturnal primates with a distinctive locomotion mode, and a large number of individuals were confiscated into captive settings due to illegal trade, making the species an ideal as a model for postural behavior monitoring. Captive animals may suffer from being housed in an inappropriate environment and may display abnormal behavior patterns. Traditional data collection methods are time-consuming and laborious, impeding efforts to improve lorises’ captive welfare and to develop effective reintroduction strategies. This study established the first human-labeled postural behavior dataset of slow lorises and used deep learning technology to recognize postural behavior based on object detection and semantic segmentation. The precision of the classification based on YOLOv5 reached 95.1%. The Dilated Residual Networks (DRN) feature extraction network showed the best performance in semantic segmentation, and the classification accuracy reached 95.2%. The results imply that computer automatic identification of postural behavior may offer advantages in assessing animal activity and can be applied to other nocturnal taxa.

Using Classical and Operant Conditioning to Train a Shifting Behavior in Juvenile False Water Cobras (Hydrodynastes gigas).

Simple SummaryPositive reinforcement training, a form of operant conditioning that rewards learners for specific behaviors to increase the frequency of that behavior, is widely used as a management tool in modern zoological facilities. This type of training offers several benefits, including improved safety, reduction in animal stress, increased choice and control, and many others. Snakes have been shown to learn using operant conditioning, yet the use of positive reinforcement training is not a widespread practice for these animals. A shaping plan, which describes the steps necessary to achieve the ultimate behavioral goal of following a target into a secondary container from their primary enclosure, was developed for false water cobras (Hydrodynastes gigas). Snakes were given food rewards for performing behaviors related to each goal detailed in the plan. Completion of this shaping plan resulted in a reduction of stress behaviors as the training progressed, as well as a decrease in time between the presentation and snakes interacting with the target.All animals have the capacity to learn through operant conditioning and other types of learning, and as a result, zoos and other animal care facilities have shifted towards the use of positive reinforcement training to shape the behavior of animals under their care. Training offers animals the choice to participate in their own husbandry routines and veterinary procedures, while also providing mental stimulation. By adopting these practices, the welfare of animals in human care has improved, but it has not been applied equally across taxa. Snakes are frequently overlooked in the discussion of choice and control in a captive setting, likely due to the historical misinterpretation of their intelligence and behavioral needs. In this study, a shaping plan was developed for 28 juvenile false water cobras (Hydrodynastes gigas), a rear-fanged venomous species, from four clutches. Snakes were rewarded with food when completing behaviors related to the ultimate goal of following a target into a shift container. The purpose of this study is to incorporate the trained behaviors in routine husbandry practices, while preventing unnecessary stress in the snakes and risk to the keeper.

Outcomes of Birds of Prey with Surgical or Traumatic Wing Amputation: A Retrospective Study from 1995 to 2017.

Surgical amputation of a limb is often required to treat raptor orthopedic injuries at rehabilitation centers. In some cases, amputation is an alternative to euthanasia if the bird's welfare is deemed appropriate under human care. The outcome for raptors maintained in a captive setting following wing amputation is poorly documented. A retrospective study was conducted in a Canadian raptor rehabilitation facility to assess the outcomes and complications observed in captive and free-living raptors with partial or complete, surgical or traumatic amputations of the wing. Data from raptors admitted to the rehabilitation center from 1995 to 2017 were reviewed. Overall, 32 records were included in this retrospective study from 11 species of raptors with surgical or traumatic wing amputations. Survival times of the 23 birds with an amputated wing (median: 1070 days, range: 68 days to 13 years and 1 month) were significantly (P = .02) longer than the survival times of the 404 non-amputated birds (median: 696 days, range 37 days to 27 years and 3 months). Complications occurred in 13 of 30 birds (43%) during the recovery period, with 9 of 30 (30%) birds developing life-threatening complications. Maladaptation to captivity was the leading cause of euthanasia during this period. After placement in captivity, 3/23 (13%) birds developed complications related to the amputation site. Based on this study, we conclude that some birds can tolerate partial or complete wing amputation, but the decision to place a bird in a captive setting should encompass the bird's ability to cope with human interaction and the availability of an adapted and safe enclosure for the animal.

Resource competition explains rare cannibalism in the wild in livebearing fishes.

Cannibalism, the act of preying on and consuming a conspecific, is taxonomically widespread, and putatively important in the wild, particularly in teleost fishes. Nonetheless, most studies of cannibalism in fishes have been performed in the laboratory. Here, we test four predictions for the evolution of cannibalism by conducting one of the largest assessments of cannibalism in the wild to date coupled with a mesocosm experiment. Focusing on mosquitofishes and guppies, we examined 17 species (11,946 individuals) across 189 populations in the wild, spanning both native and invasive ranges and including disparate types of habitats. We found cannibalism to be quite rare in the wild: most populations and species showed no evidence of cannibalism, and the prevalence of cannibalism was typically less than 5% within populations when it occurred. Most victims were juveniles (94%; only half of these appeared to have been newborn offspring), with the remaining 6% of victims being adult males. Females exhibited more cannibalism than males, but this was only partially explained by their larger body size, suggesting greater energetic requirements of reproduction likely play a role as well. We found no evidence that dispersal‐limited environments had a lower prevalence of cannibalism, but prevalence was greater in populations with higher conspecific densities, suggesting that more intense resource competition drives cannibalistic behavior. Supporting this conclusion, our mesocosm experiment revealed that cannibalism prevalence increased with higher conspecific density and lower resource levels but was not associated with juvenile density or strongly influenced by predation risk. We suggest that cannibalism in livebearing fishes is rare in the wild because preying on conspecifics is energetically costly and only becomes worth the effort when competition for other food is intense. Due to the artificially reduced cost of capturing conspecifics within confined spaces, cannibalism in captive settings can be much more frequent.

Nonhuman primate abnormal behavior: Etiology, assessment, and treatment.

Across captive settings, nonhuman primates may develop an array of abnormal behaviors including stereotypic and self-injurious behavior. Abnormal behavior can indicate a state of poor welfare, since it is often associated with a suboptimal environment. However, this may not always be the case as some behaviors can develop independently of any psychological distress, be triggered in environments known to promote welfare, and be part of an animal's coping mechanism. Furthermore, not all animals develop abnormal behavior, which has led researchers to assess risk factors that differentiate individuals in the display of these behaviors. Intrinsic risk factors that have been identified include the animal's species and genetics, age, sex, temperament, and clinical condition, while environmental risk factors include variables such as the animal's rearing, housing condition, husbandry procedures, and research experiences. To identify specific triggers and at-risk animals, the expression of abnormal behavior in captive nonhuman primates should be routinely addressed in a consistent manner by appropriately trained staff. Which behaviors to assess, what assessment methods to use, which primates to monitor, and the aims of data collection should all be identified before proceeding to an intervention and/or treatment. This article provides guidance for this process, by presenting an overview of known triggers and risk factors that should be considered, steps to design a comprehensive evaluation plan, and strategies that might be used for prevention or treatment. It also outlines the tools and processes for assessing and evaluating behavior in an appendix. This process will lead to a better understanding of abnormal behavior in captive primate colonies and ultimately to improved welfare.

Mercury concentrations, biomagnification and isotopic discrimination factors in two seabird species from the Humboldt Current ecosystem

Assessing mercury (Hg) biomagnification requires the description of prey-predator relationships, for each species and ecosystem, usually based on carbon and nitrogen isotope analyses. Here, we analyzed two seabirds from the Humboldt Current ecosystem, the Guanay cormorant (Phalacrocorax bougainvillii) and the Peruvian booby (Sula variegata), as well as their main prey, the Peruvian anchovy (Engraulis ringens). We reported Hg concentrations, Hg biomagnification (BMF) and isotopic discrimination factors (Δ13C and Δ15N) in seabird whole blood. BMFs and Δ13C in our study (on wild birds where diet was not controlled) were similar to other piscivorous seabirds previously studied in captive settings, but Δ15N were lower than most captive experiments. We observed lower Hg concentrations in Humboldt seabirds compared to other oligotrophic ecosystems, possibly due to Hg biodilution in the high biomass of the first trophic levels. This work calls for a better characterization of Hg trophic dynamics in productive upwelling ecosystems.

Effects of browsing enrichment associated with the temperature-humidity index and landscaping trees in giraffes (Giraffa camelopardalisreticulata).

Enclosure environments for captive giraffes can be improved by promoting species-specific behaviors and extending foraging behavior. To date, however, the effects of climatic (temperature-humidity index, THI) and environmental factors (landscaping trees) on the enrichment of captive settings have not been studied. Therefore, the present study explored the effects of browsing enrichment on the licking behavior of captive giraffes. From May 2019 to February 2020, three giraffes in the Kyoto City Zoo, Japan, were observed for 270h over two consecutive seasons (pre- and post-deciduous). Overall, branch foraging behavior and licking behavior were weakly and negatively correlated. In the pre-deciduous period, THI was significantly and negatively correlated with giraffe activity, and some individuals exhibited significantly reduced foraging behavior. Therefore, browsing enrichment under extreme heat may not improve giraffe rearing environments. Moreover, in the post-deciduous period, with decreased availability of leaves on landscaping trees, the non-branch foraging behavior of giraffes was significantly increased, with a corresponding significant increase in licking behavior. Therefore, landscaping trees affect the foraging behavior of captive giraffes.

A RETROSPECTIVE STUDY OF MORBIDITY AND MORTALITY IDENTIFIED AT POSTMORTEM EXAMINATION OF CAPTIVE LANGURS (TRACHYPITHECUS SPP) FROM SIX UNITED KINGDOM ZOOLOGICAL INSTITUTIONS: A 19-YEAR REVIEW.

Langurs are Asian primates belonging to the Colobinae subfamily. Langur populations are declining, with most species categorized as threatened by the International Union for Conservation of Nature. Investigation into the threats to population viability and sustainability would be beneficial but there is limited literature available on common diseases or causes of death in these species, either in captive or free-ranging settings. This study aimed to evaluate the most common causes of morbidity and mortality in Trachypithecus species submitted for postmortem examination by six United Kingdom zoological institutions between 2001 and 2020, to inform best practice husbandry guidelines. Necropsy and histopathology reports from 88 individuals of Trachypithecus species from six zoological organizations in the United Kingdom were analyzed. Species included Javan langurs (Trachypithecus auratus; n = 35), dusky langurs (Trachypithecus obscurus; n = 28), François' langurs (Trachypithecus francoisi; n = 16), purple-faced langurs (Trachypithecus vetulus; n = 4), silvered langurs (Trachypithecus cristatus; n = 4), and Phayre's langur (Trachypithecus phayrei; n = 1). Morbidities and causes of death were recorded. Gastrointestinal diseases and systemic infections were the leading causes of death (27.4% and 21.0% of cases where cause of death was known, respectively); linear foreign bodies were the most common cause of death. Interstitial pneumonia was frequently observed secondary to systemic infection. Heart abnormalities, anthracosis, and hemosiderosis were common but not directly associated with mortality. Further investigation is necessary to assess the importance of these conditions and whether they predispose to other diseases. This study provides a baseline for future research evaluating captive and free-ranging langur health and highlights husbandry practices that may decrease morbidity in these species.

WILDLIFE TOURISM: A SYNTHESIS OF PAST, PRESENT, AND FUTURE RESEARCH AGENDA

Wildlife tourism (WT) is an emerging sector of tourism, majorly meant to view and/or encounter wildlife in the wild, captive, and semi-captive settings. Because of the new emerging economies, there is an increased demand for wildlife destinations in both, developing and developed nations. However, a comprehensive study is lacking in WT. In this context, the present study seeks to bring together and discuss the key findings on WT from the present literature and propose new approaches to research using co-citation, co-authorship, and co-occurrence analyses. Further, the study also considers research on WT conducted so far like attitudes, bird-watching, conservation, economics, hunting, mammals, management, marine monitoring, negative impacts, positive impacts, captive wildlife, and guidelines. A data set is created that includes authors, article titles, citations, countries, co-authorship, institutions, publication years and sources, keywords, and abstracts by collecting the bibliographies from Scopus and Web of Science (WoS) indexed journals with keywords search “Wild Life, Jungle and Tourism.” The study collected 1,519 and used 1,259 published articles from 1990 to 2020, and analyzed employing VOS viewer software, which has enabled us to understand the relationship and structure of the literature.

Investigating factors of metabolic bone disease in baboons (Papio spp.) using museum collections.

ObjectivesTo assess manifestations of metabolic bone disease (MBD) and their potential environmental and phenotypic factors in captive and non‐captive baboon (Papio spp.) specimens.Materials and methodsOur sample consisted of 160 baboon specimens at the Smithsonian's National Museum of Natural History accessioned from 1890 to 1971. Combining cranial indicators of MBD and the museum's historical data, we examined factors contributing to likely instances of MBD. We used binomial‐family generalized linear models to assess differences in MBD frequency by environment (captive, non‐captive), specimen accession year, and skin color (light, medium, dark).ResultsIndicators of MBD were most frequently observed in captive baboons, with a decrease in MBD frequency over time. Fifteen non‐captive individuals showed indicators of MBD, which are the first published cases of MBD in non‐captive nonhuman primates (NHPs) to our knowledge. The most common MBD indicators were bone porosity (n = 35) and bone thickening/enlargement (n = 35). Fibrous osteodystrophy was observed frequently in our sample, likely relating to nutritional deficiencies. We found no association between exposed facial skin color variation and MBD.ConclusionsOur findings are consistent with historical accounts of MBD prevalence in captive facilities, especially earlier in the 20th century. A decrease in MBD prevalence later in the 20th century likely reflects improvements in housing, diet, and veterinary care in captive settings. Causes of MBD development in non‐captive baboons should be further explored, as understanding the potential health impacts that anthropogenic environments impose on NHPs is imperative as humans increasingly alter the natural world in the 21st century.

Using social network analysis to inform on the process of one-male-unit formation of hamadryas baboons (Papio hamadryas) in captivity.

Hamadryas baboons (Papio hamadryas) live in a complex multilevel social system with the one-male-unit (OMU) at the core. OMUs consist of an adult alpha male with one or several adult females, their dependent offspring and, sometimes a few follower males. Previous research has documented that OMUs form in four distinct ways in wild populations. In December 2015, Oakland Zoo introduced two juvenile males into the hamadryas baboon exhibit. At the time of this study, these males were approaching sexual maturity. The complex social structure of this species and the changing social dynamics that might result as they reach sexual maturity provide a unique opportunity to utilize social network analysis (SNA) methods to examine OMU formation in a captive setting with an eye towardpotential management strategies. SNA is a visualization method of looking at social data that allows researchers to understand sociality in terms of the importance of each individual, any subgroups, as well as the larger overall group dynamic. Behavioral and proximity data were collected over 6 months (July-December 2019). These data were then transformed into networks to analyze the two now subadult males' behavior over time. We found that one of the subadult males began the formation of his first OMU following one of the four pathways found in wild studies. Despite changing group dynamics, overall group cohesion remained unchanged. This study reveals hamadryas OMU formation patterns in captivity following the initial unit pathway as well as suggests potential welfare management issues that might arise.

Cetacean Acoustic Welfare in Wild and Managed-Care Settings: Gaps and Opportunities.

Simple SummaryWhales and dolphins in managed-care and wild settings are exposed to human-made, anthropogenic sounds of varying degrees. These sounds can lead to potential negative welfare outcomes if not managed correctly in zoos or in the open ocean. Current wild regulations are based on generally broad taxa-based hearing thresholds, but there is movement to take other contextual factors into account, partially informed by researchers familiar with work in zoological settings. In this spirit, we present more nuanced future directions for the evaluation of acoustic welfare in both wild and managed-care settings, with suggestions for how research in both domains can inform each other as a means to address the paucity of research available on this topic, especially in managed-care environments.Cetaceans are potentially at risk of poor welfare due to the animals’ natural reliance on sound and the persistent nature of anthropogenic noise, especially in the wild. Industrial, commercial, and recreational human activity has expanded across the seas, resulting in a propagation of sound with varying frequency characteristics. In many countries, current regulations are based on the potential to induce hearing loss; however, a more nuanced approach is needed when shaping regulations, due to other non-hearing loss effects including activation of the stress response, acoustic masking, frequency shifts, alterations in behavior, and decreased foraging. Cetaceans in managed-care settings share the same acoustic characteristics as their wild counterparts, but face different environmental parameters. There have been steps to integrate work on welfare in the wild and in managed-care contexts, and the domain of acoustics offers the opportunity to inform and connect information from both managed-care settings and the wild. Studies of subjects in managed-care give controls not available to wild studies, yet because of the conservation implications, wild studies on welfare impacts of the acoustic environment on cetaceans have largely been the focus, rather than those in captive settings. A deep integration of wild and managed-care-based acoustic welfare research can complement discovery in both domains, as captive studies can provide greater experimental control, while the more comprehensive domain of wild noise studies can help determine the gaps in managed-care based acoustic welfare science. We advocate for a new paradigm in anthropogenic noise research, recognizing the value that both wild and managed-care research plays in illustrating how noise pollution affects welfare including physiology, behavior, and cognition.