The rapid manufacturing of functional capillary electrophoresis (CE) chips made of polymethylmethacrylate (PMMA) based on laser processing technologies including structuring, surface functionalization and packaging was investigated. CO 2 laser-assisted micro-patterning was demonstrated to have a great potential for the fabrication of micro-channel devices and the production capability of CO 2 laser processes has been extended to micro-channels less than 30 μm width. A high reproducibility of micro-channel geometry was attained. The average deviation between the fabricated cross-section areas and the desired cross-section areas of micro-channels was better than 3%. The packaging of these micro-structured transparent polymers was successfully established with a laser transmission welding technique using high-power diode laser radiation. CE-chips with channel widths of 150, 100 and 50μm were fabricated and electro-analytically characterized. For this purpose high frequency contactless conductivity detection (CCD) was established. Detection of small ions (Li +, Na +, and K +) with concentrations down to 0.1 × 10 −3 mol/l was performed. Analytical results compare well to those obtained by conventional hot embossing and thermal welding. Furthermore, UV-assisted surface modification can be used to influence the micro-analytical performance. The pattern qualities and analytical results show that laser-based technologies have a great potential for application in flexible and cost-efficient production of polymeric microfluidic devices.