Researchers are depending more than ever on remote sensing techniques to monitor and assess the agricultural water status, as well as to estimate crop water usage or crop actual evapotranspiration. In the current work, normal and stressed baselines for irrigated wheat genotypes were developed in an arid part of the Sohag governorate, Egypt, using infrared thermometry in conjunction with weather parameters. The experiment was carried out in a randomized complete block design in the normal and drought stress conditions based on three replicates using ten bread wheat genotypes (G1–G10), including five accessions, under drought stress. A standard Class-A-Pan in the experimental field provided the daily evaporation measurements (mm/day), which was multiplied by a pan factor of 0.8 and 0.4 for normal and stressed conditions, respectively. The relationship between the vapor pressure deficit (VPD) and canopy-air temperature differences (Tc − Ta) was plotted under upper (fully stressed) and lower baseline (normal) equations. Accordingly, the crop water stress indexes (CWSIs) for the stressed and normal baselines for wheat genotypes were developed. Additionally, the intercept (b) and the slope (a) of the lower baseline equation were computed for different genotypes. The results indicate that, before applying irrigation water, the CWSI values were high in both growing seasons and under all irrigation regimes. After that, the CWSI values declined. G10 underwent stress treatment, which produced the greatest CWSI (0.975). Conversely, the G6 condition that received well-watered irrigation yielded the lowest result (−0.007). When compared to a well-watered one, the CWSI values indicated a trend toward rising stress. There existed an inverse link between the CWSI and grain yield (GY); that is, a lower CWSI resulted in better plant water conditions and a higher GY. Under standard conditions, the wheat’s highest GY was recorded in G2, 8.36 Ton/ha and a WCSI of 0.481. In contrast, the CWSI result for the stress treatment was 0.883, indicating a minimum GY of 5.25 Ton/ha. The Water Use Efficiency (WUE) results demonstrated that the stress irrigation regime produced a greater WUE value than the usual one. This study makes a significant contribution by investigating the techniques that would allow CWSI to be used to estimate irrigation requirements, in addition to determining the irrigation time.